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11. 发明申请

US20100015695A1 METHOD AND APPARATUS FOR AMPLIFYING NUCLEIC ACIDS 有权
公开(公告)号：US20100015695A1
公开(公告)日：2010-01-21
申请号：US12504213
申请日：2009-07-16
申请人： Yoon-kyoung CHO , Joon-ho KIM , Kak NAMKOONG , Geun-bae LIM , Jun-hong Min
发明人： Yoon-kyoung CHO , Joon-ho KIM , Kak NAMKOONG , Geun-bae LIM , Jun-hong Min
IPC分类号： C12M1/00
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

12. 发明申请

US20130156658A1 MICROFLUIDIC DEVICE AND METHOD OF MANUFACTURING THE SAME 审中-公开
标题翻译：微流体装置及其制造方法
公开(公告)号：US20130156658A1
公开(公告)日：2013-06-20
申请号：US13686625
申请日：2012-11-27
申请人： Joon-sub SHIM , Chin-sung PARK , Won-jong JUNG , Joon-ho KIM , Kak NAMKOONG
发明人： Joon-sub SHIM , Chin-sung PARK , Won-jong JUNG , Joon-ho KIM , Kak NAMKOONG
IPC分类号： B01L3/00 , B32B37/00
CPC分类号： B01L3/5027 , B01L3/502707 , B01L3/502738 , B01L2200/027 , B01L2200/0689 , B01L2200/12 , B01L2300/0806 , B01L2300/087 , B01L2300/123 , B01L2400/0409 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B32B37/00 , F16K99/0015 , F16K99/0059 , F16K2099/008 , F16K2099/0084
摘要： Embodiments of the disclosure describe a microfluidic device and a method of manufacturing the same. An embodiment of the microfluidic device includes a first substrate in which a micro-flow path and a valve seat protruding toward the micro-flow path are formed; a second substrate disposed to face the first substrate and in which a cavity corresponding to the valve seat is formed; and a polymer film disposed between the first substrate and the second substrate and comprising a bonding unit bonded to the first and second substrates and a variable unit having a variable shape according to pneumatic pressure of the cavity, wherein the variable unit has a curvature and is spaced apart from the valve seat when pneumatic pressure is not provided to the variable unit.
摘要翻译：本发明的实施例描述了一种微流体装置及其制造方法。微流体装置的一个实施例包括：第一基板，其中形成微流路和朝向微流路径突出的阀座; 第二基板，设置成面对第一基板，并且其中形成有与阀座对应的空腔; 以及聚合物膜，设置在所述第一基板和所述第二基板之间，并且包括结合到所述第一和第二基板的接合单元和根据所述空腔的气动压力具有可变形状的可变单元，其中所述可变单元具有曲率并且是当气动压力未提供给可变单元时，与阀座间隔开。

13. 发明申请

US20120085928A1 FLUORESCENCE DETECTION OPTICAL SYSTEM AND MULTI-CHANNEL FLUORESCENCE DETECTION SYSTEM INCLUDING THE SAME 审中-公开
标题翻译：荧光检测光学系统和包括其中的多通道荧光检测系统
公开(公告)号：US20120085928A1
公开(公告)日：2012-04-12
申请号：US13160668
申请日：2011-06-15
申请人： Wong-jong JUNG , Kak NAMKOONG , Joon-ho KIM , Won-seok CHUNG , Hyung-sok YEO
发明人： Wong-jong JUNG , Kak NAMKOONG , Joon-ho KIM , Won-seok CHUNG , Hyung-sok YEO
IPC分类号： G01N21/64
CPC分类号： G01N21/6452 , B01L3/502715 , G01N21/6428 , G01N2021/6441 , G01N2021/6478 , G01N2201/062
摘要： A fluorescence detection optical system comprises a light source which emits excitation light, a collimating lens which condenses the excitation light emitted from the light source into substantially parallel light, an objective lens which focuses the excitation light on a microchamber of a microfluidic device, an optical detector which measures an intensity of a fluorescence signal generated in the microchamber by the excitation light, a beam splitter which transmits or reflects the excitation light emitted from the light source toward the objective lens, and reflects or transmits the fluorescence signal generated in the microchamber toward the optical detector, and a beam shaping lens which is disposed between the beam splitter and the objective lens and expands an optical spot of the excitation light in one direction in accordance with a shape of the microchamber.
摘要翻译：荧光检测光学系统包括发射激发光的光源，将从光源发射的激发光聚光到基本上平行的光的准直透镜，将激发光聚焦在微流体装置的微室上的物镜，光学检测器，其通过激发光测量在微室中产生的荧光信号的强度;分束器，其将从光源发射的激发光朝向物镜透射或反射，并将微孔室中产生的荧光信号反射或透射，光学检测器和光束整形透镜，其设置在分束器和物镜之间，并根据微型仓的形状在一个方向上扩展激发光的光点。

14. 发明申请

US20100329931A1 SENSING SWITCH AND DETECTING METHOD USING THE SAME 有权
标题翻译：感应开关及其检测方法
公开(公告)号：US20100329931A1
公开(公告)日：2010-12-30
申请号：US12833095
申请日：2010-07-09
申请人： Kyu-tae YOO , Joon-ho KIM , Jun-hong MIN , Sung-ouk JUNG , Ji-na NAMGOONG , Kui-hyun KIM , Jeo-young SHIM , Kak NAMKOONG
发明人： Kyu-tae YOO , Joon-ho KIM , Jun-hong MIN , Sung-ouk JUNG , Ji-na NAMGOONG , Kui-hyun KIM , Jeo-young SHIM , Kak NAMKOONG
IPC分类号： G01N27/00
CPC分类号： G01N33/54373
摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.
摘要翻译：提供了一种感测开关和使用该感测开关的感测方法。感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

15. 发明申请

US20090170211A1 SENSING SWITCH AND DETECTING METHOD USING THE SAME 有权
标题翻译：感应开关及其检测方法
公开(公告)号：US20090170211A1
公开(公告)日：2009-07-02
申请号：US12272183
申请日：2008-11-17
申请人： Kyu-tae YOO , Joon-ho KIM , Jun-hong MIN , Sung-ouk JUNG , Ji-na NAMGOONG , Kui-hyun KIM , Jeo-young SHIM , Kak NAMKOONG
发明人： Kyu-tae YOO , Joon-ho KIM , Jun-hong MIN , Sung-ouk JUNG , Ji-na NAMGOONG , Kui-hyun KIM , Jeo-young SHIM , Kak NAMKOONG
IPC分类号： G01N27/00
CPC分类号： G01N33/54373
摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.
摘要翻译：提供了一种感测开关和使用该感测开关的感测方法。感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

16. 发明申请

US20090122828A1 METHOD FOR IDENTIFYING A BIOMOLECULE 有权
标题翻译：识别生物分子的方法
公开(公告)号：US20090122828A1
公开(公告)日：2009-05-14
申请号：US12108686
申请日：2008-04-24
申请人： Jung Im HAN , Jeo Young SHIM , Won Seok CHUNG , Kak NAMKOONG
发明人： Jung Im HAN , Jeo Young SHIM , Won Seok CHUNG , Kak NAMKOONG
IPC分类号： G01N25/02
CPC分类号： G01N27/4145
摘要： A method for identifying a biomolecule using a biomolecule detector having a field effect transistor (FET) is provided. The method comprises the steps of (a) heating a sample containing a biomolecule loaded in the detector to thereby elevate the temperature of the sample; (b) measuring electric current flowing through a channel formed between a source region and a drain region in the FET while raising the temperature in the step (a); (c) obtaining a transition temperature that is the temperature at maximum point of current variation from data measured in the step (b); and (d) identifying the biomolecule using the transition temperature obtained in the step (c).
摘要翻译：提供了使用具有场效应晶体管（FET）的生物分子检测器来鉴定生物分子的方法。该方法包括以下步骤：（a）加热装载在检测器中的生物分子的样品，从而提高样品的温度; （b）测量在步骤（a）中提高温度的同时流过形成在FET中的源极区域和漏极区域之间的沟道的电流; （c）获得作为在步骤（b）中测量的数据的电流变化的最大点处的温度的转变温度; 和（d）使用在步骤（c）中获得的转变温度鉴定生物分子。

17. 发明申请

US20090074623A1 MICROFLUIDIC DEVICE 审中-公开
标题翻译：微流体装置
公开(公告)号：US20090074623A1
公开(公告)日：2009-03-19
申请号：US12056311
申请日：2008-03-27
申请人： Chin-sung PARK , Kak NAMKOONG , Young-sun LEE , Joo-won RHEE
发明人： Chin-sung PARK , Kak NAMKOONG , Young-sun LEE , Joo-won RHEE
IPC分类号： B01L3/00
CPC分类号： B01L3/502707 , B01L7/52 , B01L2200/12 , B01L2300/0851 , B01L2300/16 , B01L2300/1827
摘要： Provided is a microfluidic device including a substrate; a chamber formed by a groove in a bottom surface of the substrate, whereby a fluid can be accommodated in the chamber; and an adhesive tape adhered to the bottom surface of the substrate, wherein the adhesive tape includes a polymer film and a silicone adhesive agent coated on the polymer film.
摘要翻译：提供了一种包括基板的微流体装置; 由衬底的底面中的凹槽形成的腔室，由此可将流体容纳在腔室中; 以及粘附到基材的底表面的粘合带，其中胶带包括聚合物膜和涂覆在聚合物膜上的硅氧烷粘合剂。

18. 发明申请

US20090057599A1 ELASTIC VALVE AND MICROFLUIDIC DEVICE INCLUDING THE SAME 有权
标题翻译：弹性阀和微流体装置，包括它们
公开(公告)号：US20090057599A1
公开(公告)日：2009-03-05
申请号：US12023197
申请日：2008-01-31
申请人： Kak NAMKOONG , Chin-sung PARK , Jin-tae KIM , Joo-won RHEE
发明人： Kak NAMKOONG , Chin-sung PARK , Jin-tae KIM , Joo-won RHEE
IPC分类号： F16K31/00 , F16K7/00
CPC分类号： F16K99/0001 , Y10T137/87434 , Y10T137/877
摘要： An elastic valve and a microfluidic device including the same are provided. The elastic valve includes a channel dented by external pressure so as to be elastically restituted; and a plurality of channel closing protrusions, which comprise elastomer as a material, which are protruded from at least one inner side surface of the channel toward an opposite inner side surface, and which are separated from each other so as not to interrupt a flow of fluids, wherein, when external pressure is applied in order to dent the channel, the plurality of channel closing protrusions are deformed so as to be elastically restituted, thereby closing the channel.
摘要翻译：提供弹性阀和包括该弹性阀的微流体装置。弹性阀包括通过外部压力凹陷以便被弹性恢复的通道; 以及多个通道闭合突起，其包括弹性体作为材料，其从所述通道的至少一个内侧表面突出到相对的内侧表面，并且彼此分离，以便不会中断流动流体，其中当施加外部压力以凹陷通道时，多个通道关闭突起变形以便被弹性地恢复，从而关闭通道。

19. 发明申请

US20110033919A1 METHOD OF REDUCING TEMPERATURE DIFFERENCE BETWEEN A PAIR OF SUBSTRATES AND FLUID REACTION DEVICE USING THE SAME 失效
标题翻译：降低基板对流与使用其的流体反应装置之间的温度差异的方法
公开(公告)号：US20110033919A1
公开(公告)日：2011-02-10
申请号：US12904557
申请日：2010-10-14
申请人： Kak NAMKOONG , Su-hyeon KIM , Jin-tae KIM , Chin-sung PARK , Young-sun LEE
发明人： Kak NAMKOONG , Su-hyeon KIM , Jin-tae KIM , Chin-sung PARK , Young-sun LEE
IPC分类号： C12M1/40
CPC分类号： B81B3/0081 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00873 , B01L3/5027 , B01L7/5255 , B01L2200/14 , B01L2200/147 , B01L2300/1805 , B01L2300/1827 , B81B2201/051 , F28F21/02 , F28F2013/006
摘要： A method of reducing a temperature difference between a high-temperature and a low-temperature substrate includes interposing a heat transfer facilitating layer which has a higher thermal conductivity than air and can hold particles between the substrates, and maintaining close contact between the high-temperature substrate, the heat transfer facilitating layer, and the low-temperature substrate, wherein formation of an air layer can be at least substantially prevented between the high-temperature substrate and the heat transfer facilitating layer, and between the low-temperature substrate and the heat transfer facilitating layer. A fluid reaction device includes a microfluidic reaction chip which accommodates a fluid, a heater, and a heat transfer facilitating layer which is interposed between the microfluidic reaction chip and the heater, the heat transfer facilitating layer has a higher thermal conductivity than air and can hold particles, wherein formation of an air layer can be prevented.
摘要翻译：降低高温和低温基板之间的温度差的方法包括：介于热传导率高于空气的传热促进层，并且可以在基板之间保持颗粒，并且保持高温基板，传热促进层和低温基板，其中至少可以在高温基板和传热促进层之间以及在低温基板和热量之间基本上防止空气层的形成转移促进层。流体反应装置包括容纳流体的微流体反应芯片，加热器和介于微流体反应芯片和加热器之间的传热促进层，传热促进层具有比空气更高的导热性并且可以保持颗粒，其中可以防止空气层的形成。

20. 发明申请

US20090098612A1 METHOD OF AMPLIFYING A TARGET NUCLEIC ACID BY ROLLING CIRCLE AMPLIFICATION 有权
标题翻译：通过旋转放大放大目标核酸的方法
公开(公告)号：US20090098612A1
公开(公告)日：2009-04-16
申请号：US12247433
申请日：2008-10-08
申请人： Joo-won RHEE , Young-sun LEE , Kak NAMKOONG , Chin-sung PARK
发明人： Joo-won RHEE , Young-sun LEE , Kak NAMKOONG , Chin-sung PARK
IPC分类号： C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6855 , C12Q2525/301 , C12Q2531/125 , C12Q2537/101 , C12Q2521/301 , C12Q2521/501
摘要： Provided is kit for and a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase,(d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
摘要翻译：提供了使用滚动循环扩增（RCA）扩增核酸的试剂盒和使用RCA形成单链环状DNA模板的核酸的方法，所述反应溶液包括：（a）两个发夹寡核苷酸，（b）靶核酸，（c）DNA连接酶，（d）内切核酸酶，（e）DNA聚合酶和（f）引物。

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