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11. 发明授权

US5756993A Mass spectrometer 失效
标题翻译：质谱仪
公开(公告)号：US5756993A
公开(公告)日：1998-05-26
申请号：US754356
申请日：1996-11-22
申请人： Kiyomi Yoshinari , Yoichi Ose , Katsuhiro Nakagawa , Yoshiaki Kato
发明人： Kiyomi Yoshinari , Yoichi Ose , Katsuhiro Nakagawa , Yoshiaki Kato
IPC分类号： G01N27/62 , G01N30/72 , H01J49/02 , H01J49/06 , H01J49/22 , H01J49/42 , H01J49/26
CPC分类号： H01J49/025 , G01N30/72
摘要： A sample separated through a pre-processor part having a gas chromatography (GC) or a liquid chromatography (LC) and a moving bed eliminating part is ionized by an ion source and mass-analyzed by a mass-analyzing part. The mass-analyzed ions are deflected and focused by a deflecting portion and a focusing portion in a deflecting and focusing part, and is detected by an ion detecting part. The result of detection is processed by a data processing part.
摘要翻译：通过具有气相色谱（GC）或液相色谱（LC）和移动床消除部分的预处理器部分分离的样品通过离子源离子化并通过质量分析部分进行质量分析。质量分析的离子被偏转部分和聚焦部分偏转和聚焦在偏转和聚焦部分中，并由离子检测部分检测。检测结果由数据处理部分处理。

12. 发明授权

US07544930B2 Tandem type mass analysis system and method 有权
标题翻译：串联式质量分析系统及方法
公开(公告)号：US07544930B2
公开(公告)日：2009-06-09
申请号：US11624248
申请日：2007-01-18
申请人： Kiyomi Yoshinari , Yasushi Terui , Toshiyuki Yokosuka , Kinya Kobayashi , Atsumu Hirabayashi
发明人： Kiyomi Yoshinari , Yasushi Terui , Toshiyuki Yokosuka , Kinya Kobayashi , Atsumu Hirabayashi
IPC分类号： H01J49/00
CPC分类号： H01J49/02 , H01J49/004
摘要： The present invention provides a tandem type mass analysis system capable of carrying out the differential analysis with high efficiency by the tandem type mass analysis. A predetermined number of m/z regions are set up for carrying out the mass analysis with the all ions included therein being dissociated collectively for each m/z region so as to obtain measurement MS2 data. By comparing the measurement MS2 data with reference MS2 data stored in a reference data base, a difference thereof is detected. For the m/z region with a differential component detected, the mass analysis is carried out collectively without dissociation for the all ions included therein so as to obtain measurement MS1 data. By comparing the measurement MS1 data with the reference MS1 data, a difference thereof is detected. From the difference thereof, a parent ion considered to be the differential component factor is presumed for carrying out the mass analysis with the same being dissociated.
摘要翻译：本发明提供一种能够通过串联型质量分析以高效率进行差示分析的串联式质量分析系统。建立预定数量的m / z区域以进行质量分析，其中包含的全部离子为每个m / z区域共同解离，以获得测量MS2数据。通过将测量MS2数据与参考数据库中存储的参考MS2数据进行比较，检测其差异。对于检测到差分成分的m / z区域，对于其中包含的所有离子，不分离地进行质量分析，以获得测量MS1数据。通过将测量MS1数据与参考MS1数据进行比较，检测其差异。从它们的差异来看，推测认为是差分成分因子的母离子用于进行相同解离的质量分析。

13. 发明申请

US20060043281A1 Mass spectrometric method and mass spectrometric system 有权
标题翻译：质谱法和质谱系统
公开(公告)号：US20060043281A1
公开(公告)日：2006-03-02
申请号：US11210965
申请日：2005-08-25
申请人： Kiyomi Yoshinari , Toshiyuki Yokosuka , Atsushi Ootake , Kinya Kobayashi , Yuichiro Hashimoto
发明人： Kiyomi Yoshinari , Toshiyuki Yokosuka , Atsushi Ootake , Kinya Kobayashi , Yuichiro Hashimoto
IPC分类号： B01D59/44 , H01J49/00
CPC分类号： H01J49/04 , G01N30/24 , G01N30/467 , G01N30/7206 , G01N30/724 , G01N30/8658 , H01J49/0027
摘要： In a method for mass spectrometry, a plurality of juxtaposed chromatography apparatus connected to a mass spectrometer start eluting at a predetermined time difference and the following mass spectrometer conducts mass spectrometry. A chromatogram in a preceding chromatography apparatus is analyzed on real time base and results of the analysis are used on real time base to change an elusion condition of a succeeding chromatography apparatus. A mass spectrometric system suitable for carrying out the method is also provided.
摘要翻译：在质谱法中，与质谱仪连接的多个并置色谱装置开始以预定的时间差洗脱，随后的质谱仪进行质谱分析。在实时基础上分析先前色谱装置中的色谱图，并将实时分析结果用于改变后续色谱装置的脱色条件。还提供了适用于实施该方法的质谱系统。

14. 发明授权

US07932486B2 Mass spectrometer system 有权
标题翻译：质谱仪系统
公开(公告)号：US07932486B2
公开(公告)日：2011-04-26
申请号：US12330374
申请日：2008-12-08
申请人： Akihiro Sano , Atsumu Hirabayashi , Yasushi Terui , Kinya Kobayashi , Kiyomi Yoshinari , Kenko Uchida , Toshiyuki Yokosuka
发明人： Akihiro Sano , Atsumu Hirabayashi , Yasushi Terui , Kinya Kobayashi , Kiyomi Yoshinari , Kenko Uchida , Toshiyuki Yokosuka
IPC分类号： H01J49/26
CPC分类号： G01N33/6851 , G01N33/6848 , H01J49/0031 , H01J49/0036 , H01J49/004
摘要： During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.
摘要翻译：在通过串联质谱法对蛋白质或肽进行结构分析期间，作为串联质谱目标，可以避免衍生自已经测量并且大量表达的蛋白质的肽离子。迄今为止难以分析的，从微量蛋白质衍生的肽可以在实时测定中作为串联质谱图目标自动确定。关于已经测量的蛋白质和来自蛋白质的肽的数据被自动存储在内部数据库中。存储的数据与测量数据高精度对照以确定同位素峰。以这种方式，可以在测量的实际时间内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。包含在MSn谱中的信息在涉及多级解离和质谱（MSn）的MSn的每个步骤中被有效地利用，使得用于确定下一个分析内容的流程和为母体离子的选择例如，MSn + 1分析可以在实时测量和高效率和精确度下进行优化。因此，用户关注的目标可以进行串联质谱，无需浪费测量。

15. 发明申请

US20050139761A1 Mass spectrometric data analyzing method, mass spectrometric data analyzing apparatus, mass spectrometric data analyzing program, and solution offering system 有权
公开(公告)号：US20050139761A1
公开(公告)日：2005-06-30
申请号：US10993495
申请日：2004-11-22
申请人： Kiyomi Yoshinari , Kinya Kobayashi , Lee Chahn
发明人： Kiyomi Yoshinari , Kinya Kobayashi , Lee Chahn
IPC分类号： G01N27/62 , G01N31/00 , H01J49/00 , H01J49/04 , H01J49/26
CPC分类号： H01J49/0036 , G06F19/16 , G06F19/703
摘要： A main object is to cope with an unknown structure substance thereby to identify the structure of a parent ion highly precisely and to derive a supposed structure. A method for analyzing mass spectrometric data is disclosed, which: acquires mass spectrometric data on an ionized sample and dissociated ions dissociated from the sample as a parent ion; derives dissociated ion candidates by analyzing the molecular orbits on the candidates of the structures of the parent ion; and displays the analytical results of the parent ion candidates and the dissociated ion candidates and compares the data of the dissociated ion candidates and the data of dissociated ions actually measured, to evaluate the structures of the parent ion candidates.

16. 发明授权

US06852972B2 Mass spectrometer 失效
标题翻译：质谱仪
公开(公告)号：US06852972B2
公开(公告)日：2005-02-08
申请号：US10446667
申请日：2003-05-29
申请人： Takashi Baba , Yuichiro Hashimoto , Kiyomi Yoshinari
发明人： Takashi Baba , Yuichiro Hashimoto , Kiyomi Yoshinari
IPC分类号： G01N27/62 , H01J49/40 , H01J49/42
CPC分类号： H01J49/424 , H01J49/004 , H01J49/401 , H01J49/427
摘要： Heavy ions are ejected earlier than light ions sequentially at almost zero energy and they are accelerated at a fixed voltage so as to be guided to a pusher of a TOF spectrometer. After ions are ejected in a procedure of giving an electric field gradient to an ion trap and linearly decreasing its RF voltage, a condition of spatially focusing ions having all mass numbers of a single point on the pusher is found. The focused ions are vertically accelerated using the pusher to perform the TOF mass spectrometry.
摘要翻译：重离子比轻离子顺序地以几乎零的能量排出，并且它们以固定电压被加速，以被引导到TOF光谱仪的推动器。在向离子阱提供电场梯度并线性地降低其RF电压的过程中排出离子之后，发现在推动器上具有单个质点数的空间聚焦的条件。使用推动器垂直加速聚焦的离子进行TOF质谱。

17. 发明申请

US20110101215A1 QUANTITATIVE ANALYSIS METHOD USING MASS SPECTROMETER 审中-公开
标题翻译：使用质谱仪的量化分析方法
公开(公告)号：US20110101215A1
公开(公告)日：2011-05-05
申请号：US12921087
申请日：2009-03-27
申请人： Atsumu Hirabayashi , Masako Ishimaru , Kiyomi Yoshinari , Naomi Manri
发明人： Atsumu Hirabayashi , Masako Ishimaru , Kiyomi Yoshinari , Naomi Manri
IPC分类号： H01J49/00
CPC分类号： G01N30/8675 , G01N30/7233 , H01J49/0009
摘要： In quantitation without using the isotope labeling technique, there is no means to detect the presence/absence and the time region of the occurrence of quantitative analysis-inhibitory factors in data for the analysis, and the reliability of the data for the analysis cannot be evaluated. Also, the error of the data due to the occurrence of the quantitative analysis-inhibitory factors cannot be evaluated. In order to solve the problems, first, an internal standard to be detected simultaneously with a component for the analysis is mixed in a mobile phase or an eluate of a liquid chromatograph; under the condition where no quantitative analysis-inhibitory factors occur, a blank sample is analyzed to acquire a mass chromatogram of ions originated from the internal standard; and the result is stored in a data storage unit. Then, a sample for the analysis is mixed to acquire data for the analysis of the sample; and the intensity of ions originated from the internal standard is compared with that of the blank sample in the analysis real time in a data analysis unit. At this time, if an inconsistency exceeding a predetermined threshold is detected, the occurrence of the quantitative analysis-inhibitory factors can be detected. Further, based on the inconsistency, the error range of the data can be given to a data set and the like.
摘要翻译：在不使用同位素标记技术的定量中，无法检测分析数据中定量分析抑制因子的发生的存在/不存在和时间区域，并且不能评估用于分析的数据的可靠性。此外，由于定量分析抑制因子的发生导致的数据误差不能被评估。为了解决上述问题，首先，将与分析用成分同时检测的内标与液相色谱仪的流动相或洗脱液混合，在没有定量分析抑制因子发生的条件下，分析空白样品以获得源自内标的离子的质谱图; 结果存储在数据存储单元中。然后，将用于分析的样本混合以获取用于样品分析的数据; 在数据分析单元中实时分析从内部标准产生的离子强度与空白样本的强度进行比较。此时，如果检测到超过预定阈值的不一致，则可以检测定量分析抑制因子的发生。此外，基于不一致性，可以向数据集等提供数据的误差范围。

18. 发明申请

US20070221836A1 Mass analysis system 审中-公开
标题翻译：质量分析系统
公开(公告)号：US20070221836A1
公开(公告)日：2007-09-27
申请号：US11698105
申请日：2007-01-26
申请人： Kinya Kobayashi , Kiyomi Yoshinari , Toshiyuki Yokosuka , Atsumu Hirabayashi
发明人： Kinya Kobayashi , Kiyomi Yoshinari , Toshiyuki Yokosuka , Atsumu Hirabayashi
IPC分类号： B01D59/44
CPC分类号： H01J49/02 , H01J49/0027
摘要： An object of the present invention is to evaluate quantitatively a peptide derived from a protein, whose analysis has been difficult so far, by analyzing a peptide ion derived from a protein already measured but having a different total ion amount as the tandem mass analysis target at the time of quantitatively evaluating a fluctuating component between different kinds of specimens by the tandem mass analysis of a protein or a peptide. In the present invention, in order to achieve the above-mentioned object, data of a derived peptide obtained by a first time measurement are stored automatically in an internal database and collated with second time measurement data highly accurately. The processing for selecting the peak of the already measured peptide with the relative amount fluctuation as the next tandem analysis target is implemented within the real time of the measurement for avoiding the analysis of a peptide without the relative amount fluctuation.
摘要翻译：本发明的目的是通过分析来自已经测量但已经具有不同总离子量的蛋白质的肽离子作为串联质量分析目标，定量评估来自蛋白质的肽，其分析迄今难以分析通过蛋白质或肽的串联质量分析定量评估不同种类的标本之间波动成分的时间。在本发明中，为了实现上述目的，将通过第一次测定得到的衍生肽的数据自动存储在内部数据库中，并与第二次测定数据进行高度准确的对照。选择具有相对量波动的已测量肽的峰作为下一串联分析目标的处理在测量的实时内实现，以避免没有相对量波动的肽的分析。

19. 发明申请

US20070187588A1 TANDEM TYPE MASS ANALYSIS SYSTEM AND METHOD 有权
标题翻译： TANDEM类型质量分析系统和方法
公开(公告)号：US20070187588A1
公开(公告)日：2007-08-16
申请号：US11624248
申请日：2007-01-18
申请人： Kiyomi Yoshinari , Yasushi Terui , Toshiyuki Yokosuka , Kinya Kobayashi , Atsumu Hirabayashi
发明人： Kiyomi Yoshinari , Yasushi Terui , Toshiyuki Yokosuka , Kinya Kobayashi , Atsumu Hirabayashi
IPC分类号： H01J49/00
CPC分类号： H01J49/02 , H01J49/004
摘要： The present invention provides a tandem type mass analysis system capable of carrying out the differential analysis with high efficiency by the tandem type mass analysis. A predetermined number of m/z regions are set up for carrying out the mass analysis with the all ions included therein being dissociated collectively for each m/z region so as to obtain measurement MS2 data. By comparing the measurement MS2 data with reference MS2 data stored in a reference data base, a difference thereof is detected. For the m/z region with a differential component detected, the mass analysis is carried out collectively without dissociation for the all ions included therein so as to obtain measurement MS1 data. By comparing the measurement MS1 data with the reference MS1 data, a difference thereof is detected. From the difference thereof, a parent ion considered to be the differential component factor is presumed for carrying out the mass analysis with the same being dissociated.
摘要翻译：本发明提供一种能够通过串联型质量分析以高效率进行差示分析的串联式质量分析系统。设定预定数量的m / z区域，用于进行质量分析，其中包含的全部离子为每个m / z区域共同解离，以获得测量MS 2的数据。通过将测量MS 2 SUP数据与存储在参考数据库中的参考MS 2 SUP数据进行比较，检测其差异。对于检测到差分成分的m / z区域，对于包含在其中的全部离子而不解离地进行质量分析，以获得测量MS 1的数据。通过将测量MS＆lt; 1＆gt;数据与参考MS＆lt; 1＆gt;数据进行比较，检测其差异。从它们的差异来看，推测认为是差分成分因子的母离子用于进行相同解离的质量分析。

20. 发明授权

US07126113B2 Mass spectrometry system 有权
标题翻译：质谱系统
公开(公告)号：US07126113B2
公开(公告)日：2006-10-24
申请号：US11041864
申请日：2005-01-25
申请人： Toshiyuki Yokosuka , Atsumu Hirabayashi , Yasushi Terui , Kinya Kobayashi , Kiyomi Yoshinari
发明人： Toshiyuki Yokosuka , Atsumu Hirabayashi , Yasushi Terui , Kinya Kobayashi , Kiyomi Yoshinari
IPC分类号： B01D59/44 , H01J49/00
CPC分类号： H01J49/0036 , H01J49/004
摘要： According to the existing mass spectrometric system, whether or not the informations are sufficient for analyzing substances (particularly proteins, sugars, etc.) cannot be judged in the process of measurement. Further, it is difficult to find out isomers having just the same mass number or compounds very close in mass only from the MS data. According to this invention, whether or not the retention time in the LC (or GC) of peptide formed at the time of enzymatic decomposition of protein coincides with the predicted retention time assumed from the amino acid sequence predicted from MS2 mass spectrometry data is judged within the actual time period of measurement, and thereby the quality of MS2 mass spectrometry data (quantity of information) is judged.
摘要翻译：根据现有的质谱系统，不能在测量过程中判断信息是否足以分析物质（特别是蛋白质，糖等）。此外，难以从MS数据中发现质量数量相同或质量非常接近的异构体。根据本发明，在蛋白质酶促分解时形成的肽的LC（或GC）中的保留时间是否与从MS2预测的氨基酸序列假定的预测保留时间一致， SUP>质谱数据在实际测量时间段内进行判断，从而判断MS质谱质谱数据（信息量）的质量。

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