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11. 发明申请

US20120042412A1 Methods And Compositions For Targeting Sequences Of Interest To The Chloroplast 有权
标题翻译：用于靶向叶绿体感兴趣的方法和组合物
公开(公告)号：US20120042412A1
公开(公告)日：2012-02-16
申请号：US13208960
申请日：2011-08-12
申请人： Henrik Albert , Linda A. Castle , Matthew Heckert , Jian Lu , Daniel L. Siehl , Yumin Tao
发明人： Henrik Albert , Linda A. Castle , Matthew Heckert , Jian Lu , Daniel L. Siehl , Yumin Tao
IPC分类号： A01H1/06 , C12N15/82 , C12N5/10 , C12N9/96 , A01H5/10 , C07K19/00 , C12N9/02 , C12N15/62 , A01H5/00
CPC分类号： C12N15/8221 , C12N9/0069 , C12N15/82 , C12N15/8274 , C12Y113/11027
摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.
摘要翻译：提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸，其中叶绿体转运肽包含具有如SEQ ID NO：1所示的叶绿体转运肽序列的氨基酸序列或生物学上活性变体或其片段，或其中叶绿体转运肽包含SEQ ID NO：58所示的序列或其活性变体或片段。还提供了编码其的嵌合多肽，以及细胞，植物细胞，植物和种子，其包含嵌合多核苷酸。组合物还包括编码与SEQ ID NO：57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。这样的序列包含如SEQ ID NO：58所示的叶绿体转运肽或其活性变体或片段。进一步提供包含这些序列的细胞，植物细胞，植物和种子。还提供了使用各种序列的方法。

12. 发明申请

US20110258741A1 WUSCHEL(WUS) GENE HOMOLOGS 有权
标题翻译： WUSCHEL（WUS）基因同源物
公开(公告)号：US20110258741A1
公开(公告)日：2011-10-20
申请号：US13168133
申请日：2011-06-24
申请人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
发明人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/29
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
摘要翻译：本发明涉及编码WUS多肽的分离的多核苷酸。本发明还提供了分离的WUS多肽。本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法包含多核苷酸的细胞。本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。

13. 发明申请

US20110047655A1 NOVEL FRT RECOMBINATION SITES AND METHODS OF USE 有权
标题翻译：新型FRT再现站点和使用方法
公开(公告)号：US20110047655A1
公开(公告)日：2011-02-24
申请号：US12893418
申请日：2010-09-29
申请人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
发明人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
IPC分类号： A01H5/00 , C12N5/10 , A01H5/10 , C12N15/82
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

14. 发明授权

US07223597B2 Anti-apoptosis genes and methods of use thereof 失效
标题翻译：抗凋亡基因及其使用方法
公开(公告)号：US07223597B2
公开(公告)日：2007-05-29
申请号：US10167015
申请日：2002-06-11
申请人： Carl R. Simmons , William J. Gordon-Kamm , Gurmukh S. Johal , Pedro A. Navarro Acevedo , Yumin Tao
发明人： Carl R. Simmons , William J. Gordon-Kamm , Gurmukh S. Johal , Pedro A. Navarro Acevedo , Yumin Tao
IPC分类号： C12N15/82 , C12N15/29
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8263 , Y02A40/146
摘要： The invention provides isolated nucleic acids and their encoded proteins that act as cell death inhibitors and methods of use thereof. The invention further provides expression cassettes, transformed host cells, transgenic plants and plant parts, and antibody compositions.
摘要翻译：本发明提供了作为细胞死亡抑制剂的分离的核酸及其编码的蛋白质及其使用方法。本发明进一步提供表达盒，转化的宿主细胞，转基因植物和植物部分以及抗体组合物。

15. 发明授权

US06800791B1 Method for delivery of proteins to plant cells 失效
标题翻译：将蛋白质递送到植物细胞的方法
公开(公告)号：US06800791B1
公开(公告)日：2004-10-05
申请号：US09570319
申请日：2000-05-12
申请人： Matthew A. Bailey , Stanton B. Gelvin , Saikat Bhattacharjee , William J. Gordon-Kamm , Keith S. Lowe , Yumin Tao
发明人： Matthew A. Bailey , Stanton B. Gelvin , Saikat Bhattacharjee , William J. Gordon-Kamm , Keith S. Lowe , Yumin Tao
IPC分类号： A01H500
CPC分类号： C12N15/62 , C07H21/04 , C07K2319/09 , C07K2319/10 , C07K2319/61 , C07K2319/80 , C12N15/8205
摘要： The invention relates to the use of a modified Agrobacterium to deliver proteins directly to plant cells. Proteins of interest are delivered to the plant host in the form of a fusion protein with the Agrobacterium virulence protein VirF. Nucleotide sequences encoding such fusion proteins of VirF and a protein of interest are provided. Also provided are bacteria modified to comprise such fusion proteins of VirF and a protein of interest. Methods of introducing such fusion proteins into a plant host are provided. The invention finds use in facilitating plant transformation and particularly in the bio-engineering of desirable traits into crop plants.
摘要翻译：本发明涉及使用改良的农杆菌将蛋白质直接递送至植物细胞。感兴趣的蛋白质以与农杆菌毒力蛋白VirF融合蛋白的形式递送至植物宿主。提供编码VirF和感兴趣蛋白质的融合蛋白的核苷酸序列。还提供了细菌修饰以包含VirF和感兴趣的蛋白质的这种融合蛋白。提供将这种融合蛋白引入植物宿主的方法。本发明用于促进植物转化，特别是在对作物植物期望的性状的生物工程中。

16. 发明授权

US09187762B2 Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity 有权
标题翻译：包含具有羟基苯基丙酮酸双加氧酶（HPPD）活性的序列的组合物和方法
公开(公告)号：US09187762B2
公开(公告)日：2015-11-17
申请号：US13208966
申请日：2011-08-12
申请人： Henrik Albert , Ericka R. Bermudez , Linda A. Castle , Yuxia Dong , Matthew J. Heckert , Jingtong Hou , Zhenglin Hou , Jian Lu , Daniel L. Siehl , Yumin Tao
发明人： Henrik Albert , Ericka R. Bermudez , Linda A. Castle , Yuxia Dong , Matthew J. Heckert , Jingtong Hou , Zhenglin Hou , Jian Lu , Daniel L. Siehl , Yumin Tao
IPC分类号： C12N15/00 , C12N5/10 , A01H5/00 , C12N15/82 , C12N9/02
CPC分类号： C12N15/8221 , C12N9/0069 , C12N15/82 , C12N15/8274 , C12Y113/11027
摘要： Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.
摘要翻译：提供了包含具有4-羟基苯基丙酮酸双加氧酶（HPPD）活性并且对HPPD抑制剂不敏感的多核苷酸和多肽的组合物和方法。还提供了具有HPPD序列的核酸构建体，植物，植物细胞，外植体，种子和谷粒。提供了使用HPPD序列的各种方法。这样的方法包括例如用于产生HPPD抑制剂耐受植物，植物细胞，外植体或种子的方法以及在含有本文公开的植物和/或种子的作物的田地中控制杂草的方法。还提供了用于鉴定另外的HPPD变体的方法。进一步提供了允许各种HPPD多肽及其变体和片段在叶绿体中表达或转运至叶绿体的各种方法和组合物。

17. 发明授权

US09139842B2 Methods and compositions for targeting sequences of interest to the chloroplast 有权
标题翻译：靶向叶绿体感兴趣序列的方法和组合物
公开(公告)号：US09139842B2
公开(公告)日：2015-09-22
申请号：US13208960
申请日：2011-08-12
申请人： Henrik Albert , Linda A. Castle , Matthew Heckert , Jian Lu , Daniel L. Siehl , Yumin Tao
发明人： Henrik Albert , Linda A. Castle , Matthew Heckert , Jian Lu , Daniel L. Siehl , Yumin Tao
IPC分类号： C12N15/82 , C12N9/02
CPC分类号： C12N15/8221 , C12N9/0069 , C12N15/82 , C12N15/8274 , C12Y113/11027
摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.
摘要翻译：提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸，其中叶绿体转运肽包含具有如SEQ ID NO：1所示的叶绿体转运肽序列的氨基酸序列或生物学上活性变体或其片段，或其中叶绿体转运肽包含SEQ ID NO：58所示的序列或其活性变体或片段。还提供了编码其的嵌合多肽，以及细胞，植物细胞，植物和种子，其包含嵌合多核苷酸。组合物还包括编码与SEQ ID NO：57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。这样的序列包含如SEQ ID NO：58所示的叶绿体转运肽或其活性变体或片段。进一步提供包含这些序列的细胞，植物细胞，植物和种子。还提供了使用各种序列的方法。

18. 发明授权

US08912392B2 Methods for altering the genome of a monocot plant cell 有权
标题翻译：改变单子叶植物细胞基因组的方法
公开(公告)号：US08912392B2
公开(公告)日：2014-12-16
申请号：US12147834
申请日：2008-06-27
申请人： L. Aleksander Lyznik , Yumin Tao , Huirong Gao
发明人： L. Aleksander Lyznik , Yumin Tao , Huirong Gao
IPC分类号： C12N15/87 , C12N15/82 , C12N9/22
CPC分类号： C12N15/8201 , A01H1/02 , C12N9/22 , C12N15/8213 , C12N15/8241 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： Methods and compositions for altering the genome of a monocot plant cell, and a monocot plant are disclosed. The methods and compositions use a double-strand break inducing agent to alter a monocot plant or plant cell genomic sequence comprising a recognition sequence for the double-strand break inducing agent.
摘要翻译：公开了用于改变单子叶植物细胞和单子叶植物的基因组的方法和组合物。所述方法和组合物使用双链断裂诱导剂来改变包含双链断裂诱导剂的识别序列的单子叶植物或植物细胞基因组序列。

19. 发明授权

US08318493B2 FRT recombination sites and methods of use 有权
标题翻译： FRT重组位点和使用方法
公开(公告)号：US08318493B2
公开(公告)日：2012-11-27
申请号：US12756211
申请日：2010-04-08
申请人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
发明人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
IPC分类号： C12N15/87 , C12N15/82
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

20. 发明授权

US07994391B2 Wuschel (WUS) gene homologs 有权
标题翻译： Wuschel（WUS）基因同系物
公开(公告)号：US07994391B2
公开(公告)日：2011-08-09
申请号：US12018292
申请日：2008-01-23
申请人： Rebecca E. Cahoon , William James Gordon-Kamm , Yumin Tao , Keith S. Lowe , Christopher Jay Scelonge
发明人： Rebecca E. Cahoon , William James Gordon-Kamm , Yumin Tao , Keith S. Lowe , Christopher Jay Scelonge
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
摘要翻译：本发明涉及编码WUS多肽的分离的多核苷酸。本发明还提供了分离的WUS多肽。本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法包含多核苷酸的细胞。本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。

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