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1. 发明授权

US09234194B2 Modified FRT recombination site libraries and methods of use 有权
标题翻译：修改的FRT重组位点文库和使用方法
公开(公告)号：US09234194B2
公开(公告)日：2016-01-12
申请号：US12706489
申请日：2010-02-16
申请人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
发明人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
IPC分类号： C40B40/08 , C12N15/10 , C12N15/82
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。还提供了包含FRT位点的文库群的试剂盒，以及制备修饰的FRT重组位点的文库的方法。重组生成修饰的FRT重组位点可用于多种目标多重核苷酸重组方法。

2. 发明授权

US08586361B2 FRT recombination sites and methods of use 有权
标题翻译： FRT重组位点和使用方法
公开(公告)号：US08586361B2
公开(公告)日：2013-11-19
申请号：US12893418
申请日：2010-09-29
申请人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
发明人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
IPC分类号： C12N15/87 , C12N15/82
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

3. 发明申请

US20110047655A1 NOVEL FRT RECOMBINATION SITES AND METHODS OF USE 有权
标题翻译：新型FRT再现站点和使用方法
公开(公告)号：US20110047655A1
公开(公告)日：2011-02-24
申请号：US12893418
申请日：2010-09-29
申请人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
发明人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
IPC分类号： A01H5/00 , C12N5/10 , A01H5/10 , C12N15/82
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

4. 发明授权

US08318493B2 FRT recombination sites and methods of use 有权
标题翻译： FRT重组位点和使用方法
公开(公告)号：US08318493B2
公开(公告)日：2012-11-27
申请号：US12756211
申请日：2010-04-08
申请人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
发明人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
IPC分类号： C12N15/87 , C12N15/82
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

5. 发明申请

US20100192263A1 Novel FRT Recombination Sites and Methods of Use 有权
标题翻译：新型FRT重组站点和使用方法
公开(公告)号：US20100192263A1
公开(公告)日：2010-07-29
申请号：US12756211
申请日：2010-04-08
申请人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
发明人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
IPC分类号： A01H5/00 , C07H21/04 , C12N5/10 , C12N5/04
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

6. 发明授权

US07736897B2 FRT recombination sites and methods of use 有权
标题翻译： FRT重组位点和使用方法
公开(公告)号：US07736897B2
公开(公告)日：2010-06-15
申请号：US11487300
申请日：2006-07-14
申请人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
发明人： Yumin Tao , Dennis L. Bidney , William J. Gordon-Kamm , Leszek A. Lyznik
IPC分类号： C12N15/87 , C12N15/82
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译：提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

7. 发明申请

US20140338070A1 METHODS AND COMPOSITIONS FOR TARGETED INTEGRATION IN A PLANT 有权
标题翻译：方法和组合在工厂中进行集中整合
公开(公告)号：US20140338070A1
公开(公告)日：2014-11-13
申请号：US14236597
申请日：2012-07-18
申请人： Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Michael W. Lassner , Zhongsen Li , Zhan-Bin Liu , Leszek A. Lyznik , David J. Peterson , Christopher Jay Scelonge
发明人： Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Michael W. Lassner , Zhongsen Li , Zhan-Bin Liu , Leszek A. Lyznik , David J. Peterson , Christopher Jay Scelonge
IPC分类号： C12N15/82
CPC分类号： C12N15/8213
摘要： Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.
摘要翻译：提供组合物和方法用于将目的多核苷酸序列靶向整合到植物或植物细胞的基因组中。方法和组合物使用核酸内切酶和内切核酸酶的识别位点与位点特异性重组位点/重组酶组合，以提供用于在植物，植物细胞或种子的基因组内建立靶位点的有效系统。一旦建立了这样的靶位点，就可以采用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。

8. 发明授权

US6110736A Site-directed recombination in plants 有权
标题翻译：植物中的定点重组
公开(公告)号：US6110736A
公开(公告)日：2000-08-29
申请号：US211408
申请日：1998-12-15
申请人： Thomas K. Hodges , Leszek A. Lyznik
发明人： Thomas K. Hodges , Leszek A. Lyznik
IPC分类号： C12N15/82 , C12N15/90 , C12N15/63 , C12N5/04 , C07H21/04
CPC分类号： C12N15/8213 , C12N15/902
摘要： DNA constructs and methods for conducting site-specific recombination in plants is described. The method utilizes FLP recombinase to excise a region of DNA flanked by a pair of directly repeated site-specific recombination sequences which are capable of interacting with a FLP recombinase.
摘要翻译：描述了用于在植物中进行位点特异性重组的DNA构建体和方法。该方法利用FLP重组酶来切除DNA区域，其侧翼是能够与FLP重组酶相互作用的一对直接重复的位点特异性重组序列。

9. 发明授权

US08017829B1 Selective expression of genes in plants 有权
标题翻译：植物中基因的选择性表达
公开(公告)号：US08017829B1
公开(公告)日：2011-09-13
申请号：US09486904
申请日：1998-09-03
申请人： John R. Snyder , Thomas K. Hodges , Leszek A. Lyznik
发明人： John R. Snyder , Thomas K. Hodges , Leszek A. Lyznik
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8217
摘要： This invention relates to a method of biocatalytically producing compounds that are commercially valuable but are detrimental to the host cells' development. The transgenic plants produced in accordance with the present invention comprise a novel controlled expression system comprising a promoter (2), a blocking sequence (a), and a structural gene (6), wherein the blocking sequence (a) is flanked by a pair of directly repeated site-specific recombination sequences (4). The blocking sequence (a) prevents expression of the structural gene (6) until removal of the blocking sequence (a) by site-specific recombinase activity encoded by a site-specific recombinase gene (12).
摘要翻译：本发明涉及生物催化生产具有商业价值但不利于宿主细胞发育的化合物的方法。根据本发明生产的转基因植物包含新颖的受控表达系统，其包含启动子（2），阻断序列（a）和结构基因（6），其中所述阻断序列（a）侧面是一对的直接重复的位点特异性重组序列（4）。阻断序列（a）通过由位点特异性重组酶基因（12）编码的位点特异性重组酶活性来阻止结构基因（6）的表达，直到去除阻断序列（a）。

10. 发明授权

US06365728B1 Regulatory element for expressing genes in plants 失效
标题翻译：在植物中表达基因的调节元件
公开(公告)号：US06365728B1
公开(公告)日：2002-04-02
申请号：US09402328
申请日：1999-11-05
申请人： Thomas K. Hodges , Leszek A. Lyznik
发明人： Thomas K. Hodges , Leszek A. Lyznik
IPC分类号： C12N1582
CPC分类号： C12N15/8216 , C07K14/415
摘要： The present invention is directed to substantially purified nucleic acids having a novel promoter sequence that expresses genes constitutively at a high level in Arabidopsis cells. The present invention is also directed to expression vectors comprising that promoter sequence and plants produced from the in vitro introduction of such expression vectors.
摘要翻译：本发明涉及具有在拟南芥细胞中以高水平组成型表达基因的新型启动子序列的基本纯化的核酸。本发明还涉及包含该启动子序列的表达载体和从体外引入这些表达载体产生的植物。

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