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11. 发明申请

WO2009136676A1 METHOD FOR SPECIFIC COVALENT COUPLING OF ANTIBODY USING A PHOTOACTIVABLE PROTEIN G VARIANT 审中-公开
标题翻译：使用光化学蛋白G变体进行抗体的特异性共价连接的方法
公开(公告)号：WO2009136676A1
公开(公告)日：2009-11-12
申请号：PCT/KR2008/002956
申请日：2008-05-27
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , JUNG, Yong won , LEE, Jeong Min
发明人： CHUNG, Bong Hyun , JUNG, Yong won , LEE, Jeong Min
IPC分类号： C07K14/315
CPC分类号： C07K14/315 , G01N33/531 , G01N33/533
摘要： The present invention relates to a protein G variant comprising a mutated Fc binding domain, which is prepared by substituting cysteine for specific residues of the Fc-binding domain of protein G, and a method for preparing the same. Further, the present invention relates to a protein G variant comprising a cysteine mutated Fc binding domain that is site-selectively modified with a UV cross-linker. Further, the present invention relates to a method for UV cross-linking the protein G variant with antibody. The present invention relates to a protein G variant-antibody conjugate that is prepared by the above method. Further, the present invention provides a method for screening or analyzing antigens using the conjugate. Furthermore, the present invention provides a biochip or biosensor fabricated by linking the protein G variant to the surface of a solid support, and a method for fabricating the same. In addition, the present invention provides a method for immobilizing antibodies and analyzing antigens using the biochip or biosensor.
摘要翻译：本发明涉及包含通过用半胱氨酸代替蛋白G的Fc结合结构域的特异性残基制备的突变Fc结合结构域的蛋白G变体及其制备方法。此外，本发明涉及包含用UV交联剂位点选择性修饰的半胱氨酸突变的Fc结合结构域的蛋白G变体。此外，本发明涉及用抗体将蛋白G变体UV交联的方法。本发明涉及通过上述方法制备的蛋白G变体 - 抗体缀合物。此外，本发明提供了使用该缀合物筛选或分析抗原的方法。此外，本发明提供通过将蛋白G变体与固体支持物的表面连接而制造的生物芯片或生物传感器及其制造方法。此外，本发明提供了使用生物芯片或生物传感器固定抗体和分析抗原的方法。

12. 发明申请

WO2010027124A1 METAL NANOPARTICLES OF VARIOUS SIZES AND SHAPES HAVING FUNCTIONAL GROUPS AND METHOD FOR PREPARING THE SAME 审中-公开
标题翻译：具有功能组的各种尺寸和形状的金属纳米颗粒及其制备方法
公开(公告)号：WO2010027124A1
公开(公告)日：2010-03-11
申请号：PCT/KR2008/006851
申请日：2008-11-20
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , CHUNG, Im Sik , SONG, Hyun Joon , SEO, Dae Ha , YOO, Choong Il
发明人： CHUNG, Bong Hyun , CHUNG, Im Sik , SONG, Hyun Joon , SEO, Dae Ha , YOO, Choong Il
IPC分类号： B82B3/00
CPC分类号： B22F1/0059 , B22F1/0018 , B22F9/24 , B22F2001/0037 , B82Y30/00
摘要： The present invention relates to metal nanoparticles of various sizes and shapes having functional groups on the surface thereof and a method for preparing the same, and more particularly, to metal nanoparticles coated with a poly(vinylpyrrolidone) copolymer having functional groups and a method for preparing the same. According to the present invention, it is possible to readily prepare metal nanoparticles of regular hexahedral, regular octahedral, spherical shapes and the like, having a particle size of 10 to 500 nm, which comprises functional groups such as hydroxyl group, amine group, carboxyl group and the like on the surface thereof so as to be able to connect various organic molecules to biomolecules, without any post treatment of the metal nanoparticles such as surface treatment, substitution and the like.
摘要翻译：本发明涉及具有表面上具有官能团的各种尺寸和形状的金属纳米颗粒及其制备方法，更具体地涉及涂覆有具有官能团的聚（乙烯基吡咯烷酮）共聚物的金属纳米颗粒和制备方法一样。根据本发明，可以容易地制备粒径为10〜500nm的规则的六面体，规则的八面体，球形等金属纳米粒子，其包括羟基，胺基，羧基等官能团组等，以便能够将各种有机分子连接到生物分子，而不对金属纳米颗粒进行任何后处理，例如表面处理，取代等。

13. 发明申请

WO2009025465A1 SURFACE PLASMON RESONANCE SENSOR USING ROTATING MIRROR 审中-公开
标题翻译：使用旋转镜的表面等离子体共振传感器
公开(公告)号：WO2009025465A1
公开(公告)日：2009-02-26
申请号：PCT/KR2008/004701
申请日：2008-08-13
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , SHIN, Yong Beom
发明人： CHUNG, Bong Hyun , SHIN, Yong Beom
IPC分类号： G01N21/25
CPC分类号： G01N21/553 , G01N21/7743 , G01N2201/0636 , G01N2201/105 , G02B5/008
摘要： Disclosed is a surface plasmon resonance sensor having a rotating mirror, including (a) an incident light source unit for oscillating a laser as incident light; (b) a polarizer for polarizing the incident light; (c) a rotating mirror having cylindrical or planar shapes for reflecting the polarized incident light to radiate disk- shaped light; (d) a light shielding film for allowing part of the disk-shaped light to pass therethrough and located adjacent to the central axis of the rotating mirror; (e) a cylinder lens for focusing the light passed through the light shielding film thereon; (f) a metal thin film for receiving the light focused on the cylinder lens to generate surface plasmon resonance; (g) a dielectric medium provided under the metal thin film; and (h) a detector for detecting the light reflected from the metal thin film. This surface plasmon resonance sensor can solve conventional interference problems of a laser being used as a light source to obtain an image for measurement of angle and two-dimensional reflected light intensity, including the deterioration of the quality of the image and the variation in the intensity of light source depending on the position of incidence.
摘要翻译：公开了一种具有旋转镜的表面等离子体共振传感器，包括：（a）用于使激光振荡的入射光源单元作为入射光; （b）用于偏振入射光的偏振器; （c）具有用于反射偏振的入射光以辐射盘形光的圆柱形或平面形状的旋转镜; （d）遮光膜，用于允许所述盘状光的一部分通过并位于所述旋转镜的中心轴线附近; （e）用于将通过遮光膜的光聚焦在其上的柱面透镜; （f）用于接收聚焦在圆柱透镜上的光以产生表面等离子体共振的金属薄膜; （g）设置在金属薄膜下方的电介质; 和（h）用于检测从金属薄膜反射的光的检测器。该表面等离子体共振传感器可以解决用作光源的激光的常规干扰问题，以获得用于测量角度和二维反射光强度的图像，包括图像质量的劣化和强度变化的光源取决于发生的位置。

14. 发明申请

WO2008056837A1 METHOD FOR FABRICATING A BIOMATERIAL ARRAY USING PHOTOREACTION 审中-公开
标题翻译：使用光电制作生物体阵列的方法
公开(公告)号：WO2008056837A1
公开(公告)日：2008-05-15
申请号：PCT/KR2006/004646
申请日：2006-11-07
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , KIM, Min-Gon , SHIN, Yong-Beom , CHUNG, Bong Hyun , AHN, Jun Hyoung , JOUNG, Hyou-Arm
发明人： KIM, Min-Gon , SHIN, Yong-Beom , CHUNG, Bong Hyun , AHN, Jun Hyoung , JOUNG, Hyou-Arm
IPC分类号： G01N33/53
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00432 , B01J2219/00596 , B01J2219/00605 , B01J2219/00659 , B01J2219/00711 , B01J2219/0072 , B01J2219/00722
摘要： The present invention relates to a method for fabricating a biomaterial array using photoreaction. The method comprises modifying a substrate surface with psoralen (PSO) to prepare a substrate having a PSO surface formed thereon, and immobilizing biomaterials ( e.g ., DNA, proteins, etc.) on the substrate having the PSO surface using photoreaction. According to the present invention, a biomaterial can be immobilized by photoreaction without separate modification of the biomaterial, thus making it possible to fabricate an array in a very simple and cost-effective manner. Also, when a biomaterial is immobilized using focused light, it is possible to fabricate an array having a very small spot size, which is impossible with the prior microarray.
摘要翻译：本发明涉及使用光反应制造生物材料阵列的方法。该方法包括用补骨脂素（PSO）修饰底物表面以制备其上形成有PSO表面的底物，并使用光反应将生物材料（例如DNA，蛋白质等）固定在具有PSO表面的基底上。根据本发明，通过光反应可以固定生物材料，而不需要对生物材料进行单独的修饰，从而可以以非常简单和成本有效的方式制备阵列。此外，当使用聚焦光固定生物材料时，可以制造具有非常小的斑点尺寸的阵列，这对于现有的微阵列是不可能的。

15. 发明申请

WO2007132998A1 LINKER MOLECULES FOR SUBSTRATE SURFACE TREATMENT AND SPECIFIC PROTEIN IMMOBILIZATION, AND METHOD FOR PREPARING THE SAME 审中-公开
标题翻译：用于基板表面处理和特定蛋白质固定的连接分子及其制备方法
公开(公告)号：WO2007132998A1
公开(公告)日：2007-11-22
申请号：PCT/KR2007/002250
申请日：2007-05-08
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , HA, Tai Hwan , JUNG, Sun Ok , CHUNG, Sang Jeon , LEE, Suh Hyun , KIM, Jung-Won
发明人： CHUNG, Bong Hyun , HA, Tai Hwan , JUNG, Sun Ok , CHUNG, Sang Jeon , LEE, Suh Hyun , KIM, Jung-Won
IPC分类号： C07K19/00
CPC分类号： C07K19/00
摘要： The present invention relates to a linker molecule for immobilizing a protein on a substrate surface and a preparation method thereof. More specifically, relates to a linker molecule, which has a functional group binding to a substrate, and a functional group binding to a protein, at both ends of a protein-immobilizing ligand, respectively, as well as a preparation method thereof and a method for immobilizing a protein through the linker molecule. The disclosed linker molecule can form a self-assembled monolayer on a substrate selected from the group consisting of gold, silver, semiconductor, glass, silicon and polymer, so as to minimize non-specific binding in a process of immobilizing a protein on the substrate. Accordingly, the linker molecule will be useful for biochips, biosensors and other supports for protein immobilization.
摘要翻译：本发明涉及用于将蛋白质固定在基材表面上的连接分子及其制备方法。更具体地，涉及分别具有与底物结合的官能团的接头分子和与蛋白质固定化配体两端的蛋白质结合的官能团，以及其制备方法和方法用于通过连接分子固定蛋白质。所公开的接头分子可以在选自金，银，半导体，玻璃，硅和聚合物的基底上形成自组装单层，以便在将蛋白质固定在基底上的过程中使非特异性结合最小化。因此，连接分子可用于生物芯片，生物传感器和用于蛋白质固定的其它载体。

16. 发明申请

WO2009054608A1 WATER SOLUBLE PHOTOCHROMIC COMPOUNDS FOR LABELING OF BIOMOLECULES AND METHOD FOR DETECTING BIOMOLECULES USING THE SAME 审中-公开
标题翻译：用于生物分子标记的水溶性光致变色化合物和使用该分析物检测生物分子的方法
公开(公告)号：WO2009054608A1
公开(公告)日：2009-04-30
申请号：PCT/KR2008/004882
申请日：2008-08-21
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , CHUNG, Sang Jeon , LEE, Suh Hyun , CHUNG, Im Sik , PARK, Hyun Kyu , LEE, Chang Soo
发明人： CHUNG, Bong Hyun , CHUNG, Sang Jeon , LEE, Suh Hyun , CHUNG, Im Sik , PARK, Hyun Kyu , LEE, Chang Soo
IPC分类号： A61K49/00
CPC分类号： G01N33/533 , A61K49/0021 , A61K49/006 , C09B11/24
摘要： The present invention relates to water-soluble photochromic compounds for labeling biomolecules and a method of detecting biomolecules using the same. More specifically, relates to water-soluble photochromic compounds for labeling biomolecules, in which a functional group rendering photochromic molecules water-soluble is linked to a functional group capable of binding to biomolecules, and a method of detecting biomolecules using the same. Because the disclosed water-soluble photochromic compounds exhibit the color corresponding to the wavelength of visible light range, they allow signals to be easily detected not only with an UV-VIS spectrophotometer but also visually. Accordingly, the photochromic compounds can be advantageously used in sensors for diagnosing diseases.
摘要翻译：本发明涉及用于标记生物分子的水溶性光致变色化合物和使用其的生物分子的检测方法。更具体地，涉及用于标记生物分子的水溶性光致变色化合物，其中使可溶于水的光致变色分子的官能团与能够结合生物分子的官能团连接，以及使用该生物分子检测生物分子的方法。由于所公开的水溶性光致变色化合物表现出与可见光范围波长相对应的颜色，所以它们不仅可以使用UV-VIS分光光度计而且可视地容易地检测信号。因此，光致变色化合物可有利地用于诊断疾病的传感器中。

17. 发明申请

WO2009044949A1 METHOD FOR PREPARING ANTIBODY MONOLAYERS WHICH HAVE CONTROLLED ORIENTATION USING PEPTIDE HYBRID 审中-公开
标题翻译：使用肽混合物制备具有控制方向的抗体单体的方法
公开(公告)号：WO2009044949A1
公开(公告)日：2009-04-09
申请号：PCT/KR2007/005106
申请日：2007-10-18
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Sang Jeon , CHUNG, Bong Hyun , JUNG, Yongwon , KANG, Hyo Jin , LEE, Jeong Min
发明人： CHUNG, Sang Jeon , CHUNG, Bong Hyun , JUNG, Yongwon , KANG, Hyo Jin , LEE, Jeong Min
IPC分类号： C07K17/00
CPC分类号： C07K17/00 , C07K7/02 , C07K7/08 , G01N33/54353 , G01N33/54393
摘要： The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinitiy. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.
摘要翻译：本发明涉及一种使用肽杂交体制备蛋白质单层的方法，更准确地说，用于蛋白质固定的肽杂交体，其通过将PEG接头和适当的反应基团引入具有对所选择的特异性亲和力的寡肽而具有改善的溶解性蛋白质类型被设计为在固体底物和固定化蛋白之间提供足够的空间，由此通过混合物处理的各种固体底物有效捕获特异性蛋白质。用于本发明的蛋白质固定化的肽杂合物有助于控制抗体在各种固体表面上的取向并固定不同来源的各种抗体或具有不同亲和力的不同同种型。因此，使用本发明的肽杂交体的表面处理技术可以有效地用于生产各种免疫传感器和免疫芯片。

18. 发明申请

WO2008016221A1 CYSTEINE-TAGGED STAPHYLOCOCCAL PROTEIN G VARIANT 审中-公开
公开(公告)号：WO2008016221A1
公开(公告)日：2008-02-07
申请号：PCT/KR2007/002996
申请日：2007-06-20
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , LEE, Jeong Min , JUNG, Sun-Ok , JUNG, Yongwon
发明人： CHUNG, Bong Hyun , LEE, Jeong Min , JUNG, Sun-Ok , JUNG, Yongwon
IPC分类号： C07K14/00 , C12N15/62
CPC分类号： C07K14/31 , C07K17/00 , C07K2319/00 , C07K2319/20 , G01N33/54366 , G01N33/54393 , G01N33/56938 , G01N33/6854 , G01N2333/31 , G01N2333/315
摘要： The present invention relates to an N-terminal cysteine-tagged Streptococcal protein G variant. Since the variant binds in a directional manner to a surface of a biochip or a biosensor, the variant provides a biochip and a biosensor having an improved antibody-immobilizing capabiltiy, compared with an untagged protein G variant.
摘要翻译：本发明涉及N-末端半胱氨酸标记的链球菌蛋白G变体。由于变体以定向方式结合生物芯片或生物传感器的表面，所以与未标记的蛋白G变体相比，该变体提供具有改进的抗体固定化能力的生物芯片和生物传感器。

19. 发明申请

WO2007142402A1 A LIVER FUNCTION TESTER AND LIVER FUNCTION TESTING SYSTEM 审中-公开
标题翻译：肝功能测试仪和肝功能测试系统
公开(公告)号：WO2007142402A1
公开(公告)日：2007-12-13
申请号：PCT/KR2007/001442
申请日：2007-03-23
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , LEE, Joong Jin , KIM, Sang Kyu , PARK, Hye Jung
发明人： CHUNG, Bong Hyun , LEE, Joong Jin , KIM, Sang Kyu , PARK, Hye Jung
IPC分类号： G01N33/49
CPC分类号： G01N27/3273
摘要： Disclosed are a liver function tester and a liver function testing system. The tester comprises a sensor strip for converting density of GPT and GOP in blood into current values, a current detection/amplification unit for detecting the current flowing in the sensor strip, and a control unit for converting the current into a digital signal and outputting the level of GPT and GOP from the digital signal. The sensor strip comprises a reaction unit, in which amino acids, reacting with GPT and GOT in the blood, and enzymes, involved in the generation of electrons from the amino acids, are fixed, and an electrode unit consisting of a plurality of working electrodes and a reference electrode, one end of each of said electrodes being connected to the reaction unit.
摘要翻译：公开了肝功能检测仪和肝功能检测系统。测试仪包括用于将血液中GPT和GOP的浓度转换为电流值的传感器条，用于检测在传感器条中流动的电流的电流检测/放大单元，以及用于将电流转换为数字信号并输出来自数字信号的GPT和GOP的电平。传感器条包括反应单元，其中与血液中的GPT和GOT反应的氨基酸和涉及从氨基酸产生电子的酶被固定，并且由多个工作电极组成的电极单元和参考电极，每个所述电极的一端连接到反应单元。

20. 发明申请

WO2009104836A1 A GLUCOSE SENSOR COMPRISING GLUCOSE OXIDASE VARIANT 审中-公开
标题翻译：包含葡萄糖氧化酶变体的葡萄糖传感器
公开(公告)号：WO2009104836A1
公开(公告)日：2009-08-27
申请号：PCT/KR2008/002213
申请日：2008-04-18
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , LEE, Chang Soo , PARK, Hye Jung , LEE, Jeong Min
发明人： CHUNG, Bong Hyun , LEE, Chang Soo , PARK, Hye Jung , LEE, Jeong Min
IPC分类号： G01N33/49 , G01N33/53 , G01N33/48 , G01N27/26
CPC分类号： C12Q1/006
摘要： The present invention relates to a glucose sensor comprising an enzyme variant that uses glucose as a substrate, in particular, a blood glucose-measuring strip sensor having a high sensitivity with a trace amount of enzyme and a high detection rate, in which the glucose oxidase commonly used in glucose sensors is genetically engineered to produce an enzyme variant tagged with a positively or negatively charged peptide or protein, and then the enzyme variant is linked to an oppositely charged self-assembled monolayer that is formed on a gold, silver or copper thin layer, and a method for manufacturing the same.
摘要翻译：本发明涉及一种葡萄糖传感器，其包含使用葡萄糖作为底物的酶变体，特别是具有微量酶和高检测率的高灵敏度的血糖测定条状传感器，其中葡萄糖氧化酶通常用于葡萄糖传感器的基因工程是为了产生用带正电荷或带负电荷的肽或蛋白质标记的酶变体，然后将酶变体连接到形成在金，银或铜薄片上的相反电荷的自组装单层层及其制造方法。

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