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11. 发明申请

WO2019149789A1 YEAST CELL CAPABLE OF SIMULTANEOUSLY FERMENTING HEXOSE AND PENTOSE SUGARS 审中-公开
公开(公告)号：WO2019149789A1
公开(公告)日：2019-08-08
申请号：PCT/EP2019/052307
申请日：2019-01-31
申请人： DSM IP ASSETS B.V.
发明人： VAN MARIS, Antonius Jeroen Adriaan , PRONK, Jacobus Thomas , VERHOEVEN, Maarten Dirk , PAPAPETRIDIS, Ioannis
IPC分类号： C12N1/18 , C12N9/12 , C12P7/08 , C12N9/90
CPC分类号： C12N1/18 , C12N9/12 , C12N9/90 , C12P7/08
摘要： The invention relates to a method for preparing a yeast which is capable of simultaneously fermenting a pentose and a hexose sugar, said method comprising: (a) providing a yeast which comprises: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway; a disruption of a gene encoding a ribulose-phosphate 3-epimerase and a disruption of a gene encoding a glucose-6-phosphate isomerase; and one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and optionally: a heterologous gene encoding an NADH- dependent 6-phosphogluconate dehydrogenase; and a disruption of one or more genes encoding an NADPH dependent 6-phosphogluconate dehydrogenase, (b) subjecting said yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate when grown on a media comprising a hexose and at least one pentose sugar, so as to obtain an evolved yeast; and optionally (c) isolating a single cell isolate from said evolved yeast; which method subsequently comprises either (d) restoring, in the (optionally isolated) evolved yeast, one or more of the disrupted genes, or: (d') identifying genetic permutations in at least part of the genome of the optionally isolated evolved yeast by genome sequencing; and (e) constructing an improved pentose and hexose-fermenting yeast comprising one or more of said genetic permutations. The invention also relates to a recombinant yeast comprising one or more heterologous genes of a pentose metabolic pathway, which yeast further comprises a gene encoding a variant of a parent (endogenous) polypeptide, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence set out in SEQ ID NO: 6, comprises at least one mutation. Such yeast can be used in ethanol fermentation with shorter fermentation times.

12. 发明申请

WO2018073107A1 EUKARYOTIC CELL COMPRISING XYLOSE ISOMERASE 审中-公开
标题翻译：包含木糖异构酶的真核细胞
公开(公告)号：WO2018073107A1
公开(公告)日：2018-04-26
申请号：PCT/EP2017/076148
申请日：2017-10-13
申请人： DSM IP ASSETS B.V. , RIJKSUNIVERSITEIT GRONINGEN
发明人： VERHOEVEN, Maarten D , LEE, Misun , VAN DEN BROEK, Marel , JANSSEN, Dirk Barend , DARAN, Jean-Marc Georges , VAN MARIS, Antonius Jeroen Adriaan , PRONK, Jacobus Thomas , BRACHER, Jasmine Melanie , DE WAAL, Paulus Petrus , DE BRUIJN, Hans Marinus Charles Johannes
IPC分类号： C07K14/395 , C12N9/92 , C12P7/06
CPC分类号： C07K14/395 , C12N1/18 , C12N1/22 , C12N1/38 , C12N9/14 , C12N9/92 , C12P7/06 , C12P7/10 , C12Y306/03008 , C12Y503/01005 , Y02E50/16 , Y02E50/17
摘要： Eukaryotic cell comprising a heterologous xylose isomerase, wherein the xylose isomerase is loaded with Mn and wherein the Mn metal loading is at least 0.10 mol Mn (mol XylA subunit) -1 , preferably about 0.30 mol Mn (mol XylA subunit) -1 .
摘要翻译：包含异源木糖异构酶的真核细胞，其中所述木糖异构酶负载有Mn，并且其中所述Mn金属负载量为至少0.10mol Mn（mol XylA亚基）-1，，优选约0.30摩尔Mn（摩尔XylA亚基）-1 。

13. 发明申请

WO2017216136A1 RECOMBINANT YEAST CELL 审中-公开
标题翻译：重组酵母细胞
公开(公告)号：WO2017216136A1
公开(公告)日：2017-12-21
申请号：PCT/EP2017/064353
申请日：2017-06-13
申请人： DSM IP ASSETS B.V.
发明人： PAPAPETRIDIS, Ioannis , VAN MARIS, Antonius Jeroen Adriaan , PRONK, Jacobus Thomas , DE WAAL, Paulus Petrus , LAURET, Niels , VERHOEVEN, Maarten D , SCHMITZ, Jozef Petrus Johannes
IPC分类号： C12N1/19 , C12N9/12 , C12N9/88 , C12N15/81 , C12P7/06
CPC分类号： C12P7/06 , C12N1/16 , C12N9/1205 , C12N9/88 , C12N15/81 , C12R1/645 , C12Y207/01019 , C12Y401/01039 , Y02E50/17
摘要： The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the "PRK promoter") that enables higher expression under anaerobic conditions than under aerobic conditions.
摘要翻译：本发明描述了功能性表达一种或多种编码核酮糖-1,5-磷酸羧化酶/加氧酶（EC4.1.1.39; Rubisco）的异源核酸序列的重组酵母细胞，以及任选的一种或更多的分子伴侣，以及一种或多种磷酸核糖激酶（EC2.7.1.19; PRK），其中磷酸核糖激酶在启动子控制下（“PRK启动子”），其在厌氧条件下比在有氧条件下

14. 发明申请

WO2009011974A1 ORGANIC ACID PRODUCTION BY FUNGAL CELLS 审中-公开
标题翻译：有机酸生产真菌
公开(公告)号：WO2009011974A1
公开(公告)日：2009-01-22
申请号：PCT/US2008/064103
申请日：2008-05-19
申请人： MICROBIA PRECISION ENGINEERING, INC. , TATE & LYLE INGREDIENTS AMERICAS, INC. , WINKLER, Aaron Adriaan , DE HULSTER, Abraham Frederik , VAN DIJKEN, Johannes Pieter , PRONK, Jacobus Thomas , TRUEHEART, Joshua , MADDEN, Kevin T. , GANCEDO, Carlos , FLORES, Carmen-lisset , VAN MARIS, Antonius Jeroen Adriaan , HARRISON, Jacob C.
发明人： WINKLER, Aaron Adriaan , DE HULSTER, Abraham Frederik , VAN DIJKEN, Johannes Pieter , PRONK, Jacobus Thomas , TRUEHEART, Joshua , MADDEN, Kevin T. , GANCEDO, Carlos , FLORES, Carmen-lisset , VAN MARIS, Antonius Jeroen Adriaan , HARRISON, Jacob C.
IPC分类号： C12P7/46 , C12P7/40
CPC分类号： C12N15/52 , A23L5/00 , A23L31/00 , A23V2002/00 , C07K14/37 , C12P7/46
摘要： Improved systems for the biological production of organic acids are described.
摘要翻译：描述了用于生物制备有机酸的改进的系统。

15. 发明申请

WO2011010923A1 FERMENTATIVE GLYCEROL-FREE ETHANOL PRODUCTION 审中-公开
标题翻译：发酵无果糖乙醇生产
公开(公告)号：WO2011010923A1
公开(公告)日：2011-01-27
申请号：PCT/NL2010/050475
申请日：2010-07-23
申请人： TECHNISCHE UNIVERSITEIT DELFT , PRONK, Jacobus Thomas , VAN MARIS, Antonius Jeroen Adriaan , GUADALUPE MEDINA, Victor Gabriel
发明人： PRONK, Jacobus Thomas , VAN MARIS, Antonius Jeroen Adriaan , GUADALUPE MEDINA, Victor Gabriel
IPC分类号： C12N1/18 , C12N9/02 , C12P7/06 , C12P7/10 , C12N15/53
CPC分类号： C12N15/815 , C12N1/18 , C12N9/0006 , C12N9/0008 , C12N9/93 , C12P7/06 , C12P7/10 , C12Y101/01001 , C12Y101/01008 , C12Y102/0101 , C12Y602/01001 , Y02E50/16 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH- dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+- dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
摘要翻译：本发明涉及酵母细胞，特别是重组酵母细胞，NADH依赖性甘油合成所需的缺乏酶活性的细胞或相对于NADH-依赖性甘油合成具有降低的酶活性的细胞与其相应的野生型酵母细胞，该细胞包含编码NAD +依赖性乙酰化乙醛脱氢酶（EC 1.2.1.10）活性的一种或多种异源核酸序列。本发明还涉及根据本发明的细胞在制备乙醇中的用途。

16. 发明申请

WO2008144626A1 MALIC ACID PRODUCTION IN RECOMBINANT YEAST 审中-公开
标题翻译：重组酵母中的恶臭酸生产
公开(公告)号：WO2008144626A1
公开(公告)日：2008-11-27
申请号：PCT/US2008/064078
申请日：2008-05-19
申请人： MICROBIA PRECISION ENGINEERING, INC. , TATE & LYLE INGREDIENTS AMERICAS, INC. , WINKLER, Aaron Adriaan , DE HULSTER, Abraham Frederik , VAN DIJKEN, Johannes Pieter , PRONK, Jacobus Thomas , TRUEHEART, Joshua , MADDEN, Kevin T. , HARRISON, Jacob C.
发明人： WINKLER, Aaron Adriaan , DE HULSTER, Abraham Frederik , VAN DIJKEN, Johannes Pieter , PRONK, Jacobus Thomas , TRUEHEART, Joshua , MADDEN, Kevin T. , HARRISON, Jacob C.
IPC分类号： C12P7/46 , C12N1/16
CPC分类号： C12P7/46
摘要： The present disclosure relates to modified yeast, wherein the yeast has reduced pyruvate decarboxylase polypeptide (PDC) activity and methods of using such yeast to produce malic and/or succinic acid.
摘要翻译：本公开涉及修饰的酵母，其中酵母具有还原的丙酮酸脱羧酶多肽（PDC）活性和使用这种酵母产生苹果酸和/或琥珀酸的方法。

17. 发明公开

EP3620516A1 EUKARYOTIC CELL WITH INCREASED PRODUCTION OF FERMENTATION PRODUCT 无效
公开(公告)号：EP3620516A1
公开(公告)日：2020-03-11
申请号：EP19198880.7
申请日：2016-10-03
申请人： DSM IP Assets B.V.
发明人： PAPAPETRIDIS, Ioannis , PRONK, Jacobus Thomas , VAN MARIS, Antonius Jeroen Adriaan , KLAASSEN, Paul
IPC分类号： C12N9/04 , C12N9/02 , C12N9/12 , C12N9/18 , C12N9/00 , C12P7/06 , C12P7/10
摘要： The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding:
a) D-glucose-6-phosphate dehydrogenase and/or
b) 6-phosphogluconate dehydrogenase; and/or
c) glucose dehydrogenase, gluconolactonase and gluconate kinase,
wherein a), b) and glucose dehydrogenase in c) are NAD + dependent.

18. 发明公开

EP3559246A1 IMPROVED YEAST STRAINS FOR ETHANOL PRODUCTION 有权转让
公开(公告)号：EP3559246A1
公开(公告)日：2019-10-30
申请号：EP17829177.9
申请日：2017-12-18
申请人： DSM IP Assets B.V.
发明人： PAPAPETRIDIS, Ioannis , VAN MARIS, Antonius Jeroen Adriaan , PRONK, Jacobus Thomas
IPC分类号： C12P7/06 , C12N1/19

19. 发明授权

EP2456851B1 FERMENTATIVE GLYCEROL-FREE ETHANOL PRODUCTION 有权转让
公开(公告)号：EP2456851B1
公开(公告)日：2018-10-17
申请号：EP10740412.1
申请日：2010-07-23
申请人： DSM IP Assets B.V.
发明人： PRONK, Jacobus Thomas , VAN MARIS, Antonius Jeroen Adriaan , GUADALUPE MEDINA, Victor Gabriel
IPC分类号： C12N1/18 , C12N9/02 , C12N9/04 , C12P7/06 , C12P7/10 , C12N15/53
CPC分类号： C12N15/815 , C12N1/18 , C12N9/0006 , C12N9/0008 , C12N9/93 , C12P7/06 , C12P7/10 , C12Y101/01001 , C12Y101/01008 , C12Y102/0101 , C12Y602/01001 , Y02E50/16 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD + -dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.

20. 发明公开

EP3710469A1 POLYPEPTIDES WITH IMPROVED ARABINOSE TRANSPORT SPECIFICITY 有权
公开(公告)号：EP3710469A1
公开(公告)日：2020-09-23
申请号：EP18803387.2
申请日：2018-11-12
申请人： DSM IP Assets B.V.
发明人： VAN MARIS, Antonius Jeroen Adriaan , PRONK, Jacobus Thomas , VERHOEVEN, Maarten Dirk
IPC分类号： C07K14/435 , C12N15/81

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