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1. 发明专利

JP2009112320A Production of carotenoid in oleaginous yeast and fungi 审中-公开
标题翻译：在伊利诺伊州和真菌生产卡培菌
公开(公告)号：JP2009112320A
公开(公告)日：2009-05-28
申请号：JP2009054243
申请日：2009-03-06
申请人： Microbia Precision Engineering Inc , マイクロビアプレシジョンエンジニアリング, インコーポレイテッド
发明人： BAILEY RICHARD , MADDEN KEVIN T , TRUEHEART JOSHUA
IPC分类号： C12N1/15 , C12N15/09 , C12P23/00
CPC分类号： C12N15/80 , A23L31/00 , C12P23/00
摘要： PROBLEM TO BE SOLVED: To provide a recombinant fungus. SOLUTION: The recombinant fungus is characterized by: a. the fungus is oleaginous in that it can accumulate lipid to at least about 20% of its dry cell weight; and b. the fungus produces at least one carotenoid, and can accumulate the produced carotenoid to at least about 1% of its dry cell weight; wherein the fungus comprises at least one modification selected from the group consisting of carotenogenic modifications, oleaginic modifications, and combinations thereof, and wherein the at least one modification alters oleaginicity of the fungus, confers to the fungus oleaginy, confers to the fungus the ability to produce the at least one carotenoid to a level at least about 1% of its dry cell weight, or confers to the fungus the ability to produce at least one carotenoid which the fungus does not naturally produce. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供重组真菌。解决方案：重组真菌的特征在于：a。真菌是油性的，因为它可以将脂质积聚至其干细胞重量的至少约20％; 和b。真菌产生至少一种类胡萝卜素，并且可以将生成的类胡萝卜素积累至其干细胞重量的至少约1％; 其中所述真菌包含至少一种选自以下的修饰：胡萝卜素变性，油性变体及其组合，并且其中所述至少一种修饰改变真菌的油性，赋予真菌含油，赋予真菌赋予真菌的能力，产生至少一种类胡萝卜素至其干细胞重量的至少约1％的水平，或赋予真菌产生至少一种类似物的能力，该类胡萝卜素不是天然产生的。版权所有（C）2009，JPO＆INPIT

2. 发明申请

WO2008144626A1 MALIC ACID PRODUCTION IN RECOMBINANT YEAST 审中-公开
标题翻译：重组酵母中的恶臭酸生产
公开(公告)号：WO2008144626A1
公开(公告)日：2008-11-27
申请号：PCT/US2008/064078
申请日：2008-05-19
申请人： MICROBIA PRECISION ENGINEERING, INC. , TATE & LYLE INGREDIENTS AMERICAS, INC. , WINKLER, Aaron Adriaan , DE HULSTER, Abraham Frederik , VAN DIJKEN, Johannes Pieter , PRONK, Jacobus Thomas , TRUEHEART, Joshua , MADDEN, Kevin T. , HARRISON, Jacob C.
发明人： WINKLER, Aaron Adriaan , DE HULSTER, Abraham Frederik , VAN DIJKEN, Johannes Pieter , PRONK, Jacobus Thomas , TRUEHEART, Joshua , MADDEN, Kevin T. , HARRISON, Jacob C.
IPC分类号： C12P7/46 , C12N1/16
CPC分类号： C12P7/46
摘要： The present disclosure relates to modified yeast, wherein the yeast has reduced pyruvate decarboxylase polypeptide (PDC) activity and methods of using such yeast to produce malic and/or succinic acid.
摘要翻译：本公开涉及修饰的酵母，其中酵母具有还原的丙酮酸脱羧酶多肽（PDC）活性和使用这种酵母产生苹果酸和/或琥珀酸的方法。

3. 发明申请

WO2009032987A1 ISOLATION OF PELLET-FORMING MICROORGANISMS 审中-公开
标题翻译：颗粒形成微生物的分离
公开(公告)号：WO2009032987A1
公开(公告)日：2009-03-12
申请号：PCT/US2008/075352
申请日：2008-09-05
申请人： MICROBIA PRECISION ENGINEERING, INC. , TRUEHEART, Joshua , LIEVENSE, Jefferson, Clay , IVERSON, Sara
发明人： TRUEHEART, Joshua , LIEVENSE, Jefferson, Clay , IVERSON, Sara
IPC分类号： C12N15/01 , C12N1/00
CPC分类号： G01N21/6486 , C12N1/14 , C12N1/20 , C12N15/01 , C12P7/40 , C12Q1/04
摘要： The present disclosure provides technologies for identifying, characterizing and/or sorting pellet-forming microorganisms such as bacteria and/or fungi (e.g., yeast) in liquid culture, and particularly under fermentation conditions. In some embodiments, the pellet- forming microorganisms produce one or more commercial products. For example, in some embodiments the pellet-forming microorganisms produce one or more organic acids, carotenoid compounds, essential fatty acids, industrial enzymes, active pharmaceuticals, extracellular carbohydrates, and insecticidal compounds, etc. In many embodiments, the organisms are sorted while in their pellet form.
摘要翻译：本公开提供用于在液体培养物中，特别是在发酵条件下鉴定，表征和/或分选颗粒形成微生物如细菌和/或真菌（例如酵母）的技术。在一些实施方案中，颗粒形成微生物产生一种或多种商业产品。例如，在一些实施方案中，颗粒形成微生物产生一种或多种有机酸，类胡萝卜素化合物，必需脂肪酸，工业酶，活性药物，细胞外碳水化合物和杀虫化合物等。在许多实施方案中，生物体在他们的颗粒形式。

4. 发明申请

WO2009011974A1 ORGANIC ACID PRODUCTION BY FUNGAL CELLS 审中-公开
标题翻译：有机酸生产真菌
公开(公告)号：WO2009011974A1
公开(公告)日：2009-01-22
申请号：PCT/US2008/064103
申请日：2008-05-19
申请人： MICROBIA PRECISION ENGINEERING, INC. , TATE & LYLE INGREDIENTS AMERICAS, INC. , WINKLER, Aaron Adriaan , DE HULSTER, Abraham Frederik , VAN DIJKEN, Johannes Pieter , PRONK, Jacobus Thomas , TRUEHEART, Joshua , MADDEN, Kevin T. , GANCEDO, Carlos , FLORES, Carmen-lisset , VAN MARIS, Antonius Jeroen Adriaan , HARRISON, Jacob C.
发明人： WINKLER, Aaron Adriaan , DE HULSTER, Abraham Frederik , VAN DIJKEN, Johannes Pieter , PRONK, Jacobus Thomas , TRUEHEART, Joshua , MADDEN, Kevin T. , GANCEDO, Carlos , FLORES, Carmen-lisset , VAN MARIS, Antonius Jeroen Adriaan , HARRISON, Jacob C.
IPC分类号： C12P7/46 , C12P7/40
CPC分类号： C12N15/52 , A23L5/00 , A23L31/00 , A23V2002/00 , C07K14/37 , C12P7/46
摘要： Improved systems for the biological production of organic acids are described.
摘要翻译：描述了用于生物制备有机酸的改进的系统。

5. 发明公开

EP2155885A1 MALIC ACID PRODUCTION IN RECOMBINANT YEAST 审中-公开
标题翻译：重组酵母中的苹果酸生产
公开(公告)号：EP2155885A1
公开(公告)日：2010-02-24
申请号：EP08780672.5
申请日：2008-05-19
申请人： Microbia Precision Engineering, Inc. , Tate & Lyle Ingredients Americas, Inc.
发明人： WINKLER, Aaron Adriaan , DE HULSTER, Abraham Frederik , VAN DIJKEN, Johannes Pieter , PRONK, Jacobus Thomas , TRUEHEART, Joshua , MADDEN, Kevin T. , HARRISON, Jacob C. , FLORES, Carmen-Lisset , GANCEDO, Carlos
IPC分类号： C12P7/46 , C12N1/16
CPC分类号： C12P7/46
摘要： The present disclosure relates to modified yeast, wherein the yeast has reduced pyruvate decarboxylase polypeptide (PDC) activity and methods of using such yeast to produce malic and/or succinic acid.
摘要翻译：本公开涉及修饰的酵母，其中所述酵母具有降低的丙酮酸脱羧酶多肽（PDC）活性和使用这种酵母来产生苹果酸和/或琥珀酸的方法。

6. 发明公开

EP2158323A1 ORGANIC ACID PRODUCTION BY FUNGAL CELLS 审中-公开
标题翻译：有机酸BY蘑菇细胞产生
公开(公告)号：EP2158323A1
公开(公告)日：2010-03-03
申请号：EP08826428.8
申请日：2008-05-19
申请人： Microbia Precision Engineering, Inc. , Tate & Lyle Ingredients Americas, Inc.
发明人： WINKLER, Aaron Adriaan , DE HULSTER, Abraham Frederik , VAN DIJKEN, Johannes Pieter , PRONK, Jacobus Thomas , TRUEHEART, Joshua , MADDEN, Kevin T. , GANCEDO, Carlos , FLORES, Carmen-lisset , VAN MARIS, Antonius Jeroen Adriaan , HARRISON, Jacob C.
IPC分类号： C12P7/46 , C12P7/40
CPC分类号： C12N15/52 , A23L5/00 , A23L31/00 , A23V2002/00 , C07K14/37 , C12P7/46
摘要： Improved systems for the biological production of organic acids are described.

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