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11. 发明申请

WO2009065778A1 SUCCINIC ACID PRODUCTION IN A EUKARYOTIC CELL 审中-公开
标题翻译：在一个欧洲细胞中生产酸中毒
公开(公告)号：WO2009065778A1
公开(公告)日：2009-05-28
申请号：PCT/EP2008/065583
申请日：2008-11-14
申请人： DSM IP ASSETS B.V. , VERWAAL, René , WU, Liang , DAMVELD, Robbertus, Antonius , SAGT, Cornelis, Maria, Jacobus
发明人： VERWAAL, René , WU, Liang , DAMVELD, Robbertus, Antonius , SAGT, Cornelis, Maria, Jacobus
IPC分类号： C12N15/53 , C12N9/02 , C12P7/46
CPC分类号： C12P7/46 , C12N9/001 , C12N9/88 , C12N15/81 , C12N15/815 , Y02P20/52
摘要： The present invention relates to a recombinant eukaryotic cell selected from a yeast of a filamentous fungus comprising a nucleotide sequence encoding a NAD(H)-dependent fumarate reductase that catalyses the conversion of fumaric acid to succinic acid. The invention further relates to a process for the production of succinic acid wherein the eukaryotic cell according to the present invention is used.
摘要翻译：本发明涉及选自丝状真菌酵母的重组真核细胞，其包含编码NAD（H） - 依赖性富马酸酯还原酶的核苷酸序列，其催化富马酸转化为琥珀酸。本发明还涉及生产琥珀酸的方法，其中使用本发明的真核细胞。

12. 发明申请

WO2009065777A1 DICARBOXYLIC ACID PRODUCTION IN A FILAMENTOUS FUNGUS 审中-公开
标题翻译：真菌真菌中的二羧酸生产
公开(公告)号：WO2009065777A1
公开(公告)日：2009-05-28
申请号：PCT/EP2008/065582
申请日：2008-11-14
申请人： DSM IP ASSETS B.V. , VERWAAL, René , WU, Liang , DAMVELD, Robbertus, Antonius , SAGT, Cornelis, Maria, Jacobus
发明人： VERWAAL, René , WU, Liang , DAMVELD, Robbertus, Antonius , SAGT, Cornelis, Maria, Jacobus
IPC分类号： C12N15/60 , C12N9/88 , C12P7/46
CPC分类号： C12P7/46 , C12N9/88
摘要： The present invention relates to a recombinant fungus comprising an enzyme which catalyses the conversion of malic acid to fumaric acid in the cytosol. The invention further relates to a process for the production of a dicarboxylic acid such as fumaric acid and succinic acid, wherein the recombinant fungus is used.
摘要翻译：本发明涉及一种重组真菌，其包含催化苹果酸在胞质溶胶中转化为富马酸的酶。本发明还涉及生产二羧酸如富马酸和琥珀酸的方法，其中使用重组真菌。

13. 发明申请

WO2015181310A2 ITACONIC ACID AND ITACONATE METHYLESTER AND DIMETHYLESTER PRODUCTION 审中-公开
标题翻译：异柠檬酸和甲基丙烯酸甲酯和二甲醚生产
公开(公告)号：WO2015181310A2
公开(公告)日：2015-12-03
申请号：PCT/EP2015/061882
申请日：2015-05-28
申请人： DSM IP ASSETS B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes Andries
IPC分类号： C12N15/63
CPC分类号： C12P7/44 , C07K14/395 , C12N9/0008 , C12N9/1025 , C12N9/1029 , C12N9/1217 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/62 , C12Y203/03001 , C12Y401/01006 , C12Y401/01031 , C12Y401/01032 , C12Y604/01001
摘要： The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: - a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and - a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.
摘要翻译：本发明涉及能够产生衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯中的一种或多种的重组酵母细胞。本发明还涉及能够产生衣康酸并且过表达的重组酵母细胞： - 编码具有顺 - 乌头酸脱羧酶活性的多肽的核酸; 以及 - 编码催化向乙酰辅酶A反应的多肽的核酸。这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯的方法。

14. 发明申请

WO2014202616A2 RASAMSONIA GENE AND USE THEREOF 审中-公开
标题翻译： RASAMSONIA基因及其用途
公开(公告)号：WO2014202616A2
公开(公告)日：2014-12-24
申请号：PCT/EP2014/062737
申请日：2014-06-17
申请人： DSM IP ASSETS B.V.
发明人： WU, Liang
IPC分类号： C07K14/37
CPC分类号： C07K14/37
摘要： The invention relates to apolypeptide which comprises (a) an amino acid sequence set out in SEQ ID NO: 20501 to 32240; (b) an amino acid sequence encoded by the nucleic acid sequence of SEQ ID NO: 1 to 20500; (c) or a variant polypeptide, wherein the variant polypeptide (i) has at least 70% sequence identity with the sequence set out in SEQ ID NO: 20501 to 32240, or (ii) has an amino acid sequence that differs in, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12 amino acids from the amino acid sequence of SEQ ID NO: 20501 to 32240. The invention also relates to methods for using the polypeptide in industrial processes. 1 Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
摘要翻译：本发明涉及多肽，其包含（a）SEQ ID NO：20501至32240所示的氨基酸序列; （b）由SEQ ID NO：1至20500的核酸序列编码的氨基酸序列; （c）或变体多肽，其中所述变体多肽（i）与SEQ ID NO：20501至32240所示的序列具有至少70％的序列同一性，或（ii）具有不同于2的氨基酸序列，3,4,5,6,7,8,9,10,11或12个氨基酸。本发明还涉及在工业过程中使用该多肽的方法。本发明还包括用适于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。

15. 发明申请

WO2013110673A1 DITERPENE PRODUCTION 审中-公开
标题翻译：地面生产
公开(公告)号：WO2013110673A1
公开(公告)日：2013-08-01
申请号：PCT/EP2013/051262
申请日：2013-01-23
申请人： DSM IP ASSETS B.V.
发明人： LEHMANN, Martin , TRUEHEART, Joshua , ZWARTJENS, Priscilla , WU, Liang , BOER, Viktor , SAGT, Cornelis, Maria, Jacobus , KUMAR, Manoj , MEIJRINK, Bernard
IPC分类号： A23L1/236 , C12N9/02 , C12N9/88 , C12N9/90 , C12P5/00
CPC分类号： C12P19/56 , A23K20/10 , A23K20/121 , A23K20/163 , A23L2/60 , A23L27/36 , A23V2002/00 , C07H15/256 , C12N9/0073 , C12N9/1051 , C12N9/88 , C12N9/90 , C12N15/52 , C12P5/007 , C12Y114/13078 , C12Y204/01035 , C12Y402/03019 , C12Y505/01013
摘要： The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.
摘要翻译：本发明涉及包含一个或多个核苷酸序列的重组微生物，所述核苷酸序列编码：具有入 - copalyl焦磷酸合成酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有山梨酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力。重组微生物还可能能够表达一种或多种UDP-葡糖基转移酶，使得微生物能够产生一种或多种甜菊糖苷。

16. 发明申请

WO2015181312A2 CELLS FOR ITACONIC ACID PRODUCTION 审中-公开
标题翻译：用于生产酸的细胞
公开(公告)号：WO2015181312A2
公开(公告)日：2015-12-03
申请号：PCT/EP2015/061884
申请日：2015-05-28
申请人： DSM IP ASSETS B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes Andries , GELDER, VAN, Marina
IPC分类号： C12N15/52 , C12P7/46 , C12P7/62
CPC分类号： C12P7/62 , C12N15/52 , C12P7/46
摘要： The present invention relates to a recombinant cell which is capable of producing one or more of itaconic acid,4-methyl itaconate or 1-methyl itaconate, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. A recombinant yeast cell which is capable of producing itaconic acid and which over expresses: -a nucleic 1 acid encoding a polypeptide having cis-aconitate decarboxylase activity; and-one or more nucleic acids encoding polypeptides which separately or together catalyze a reaction towards acetyl CoA,wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans- aconitate methyltransferase. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
摘要翻译：本发明涉及能够产生衣康酸，衣康酸4-甲酯或衣康酸1-甲酯中的一种或多种的重组细胞，其中所述重组微生物已经在其基因组中被修饰，从而导致其中的缺陷产生反式乌头碱甲基转移酶。能够产生衣康酸并且过表达的重组酵母细胞： - 编码具有顺 - 乌头酸脱羧酶活性的多肽的核酸1酸; 一个或多个编码多肽的核酸，其单独或一起催化向乙酰CoA的反应，其中所述重组微生物已经在其基因组中被修饰，从而导致转基因甲基转移酶的产生缺陷。这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯或衣康酸1-甲酯的方法。

17. 发明申请

WO2015181311A1 PROCESS FOR PRODUCING ITACONIC ACID AND ITACONIC ACID ESTERS UNDER ANAEROBIC CONDITIONS 审中-公开
标题翻译：在厌氧条件下生产异柠檬酸和酸性酯的方法
公开(公告)号：WO2015181311A1
公开(公告)日：2015-12-03
申请号：PCT/EP2015/061883
申请日：2015-05-28
申请人： DSM IP ASSETS B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes Andries , BROERS, Nicolette Jasmijn , HARTMANS, Sybe
IPC分类号： C12P7/46 , C12P7/62
CPC分类号： C12P7/62 , C12P7/46
摘要： The present invention relates to a method for the production of itaconic acid or an ester of itaconic acid, which method comprises fermenting a recombinant cell capable of producing itaconic acid or an ester of itaconic acid in a suitable fermentation medium under anaerobic conditions, wherein the itaconic acid or ester of itaconic acid is produced.
摘要翻译：本发明涉及一种生产衣康酸或衣康酸酯的方法，该方法包括在厌氧条件下，在合适的发酵培养基中发酵能够生产衣康酸或衣康酸酯的重组细胞，其中衣康酸产生酸或衣康酸的酯。

18. 发明申请

WO2014080024A2 ITACONIC ACID AND ITACONATE METHYLESTER PRODUCTION 审中-公开
标题翻译：意大利酸和意大利甲醇生产
公开(公告)号：WO2014080024A2
公开(公告)日：2014-05-30
申请号：PCT/EP2013/074658
申请日：2013-11-25
申请人： DSM IP ASSETS B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes, Andries
IPC分类号： C12N15/10 , C12N15/52 , C40B40/06 , C40B40/08 , C12P7/46
CPC分类号： C12P7/46 , C12N9/88 , C12N15/81 , C12P7/62 , C12Y401/01006
摘要： The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate or 1-methyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
摘要翻译：本发明涉及能够产生衣康酸4-甲酯或衣康酸1-甲酯中的一种或多种的重组酵母细胞。本发明还涉及能够产生衣康酸并且过表达编码具有顺乌头酸脱羧酶活性的多肽的核酸的重组酵母细胞; 和编码催化对乙酰辅酶A的反应的多肽的核酸。这些重组酵母细胞可以用于生产衣康酸，衣康酸4-甲酯或衣康酸1-甲酯的方法中。

19. 发明授权

EP2806754B1 DITERPENE PRODUCTION 无效
公开(公告)号：EP2806754B1
公开(公告)日：2018-10-24
申请号：EP13700929.6
申请日：2013-01-23
申请人： DSM IP Assets B.V.
发明人： KUMAR, Manoj , VAN DEN BERG, Marco Alexander , BOER, Viktor Marius , LAWRENCE, Adam G. , LEHMANN, Martin , MEIJRINK, Bernard , SAGT, Cornelis Maria Jacobus , TRUEHART, Joshua , WU, Liang , ZWARTJENS, Priscilla
IPC分类号： C12N9/02 , C12N9/10 , C12N9/90 , A23L2/60 , C12P5/00 , C07H15/256 , C12P19/56 , C12N15/52 , A23K20/121 , A23K20/163 , A23K20/10 , A23L27/30 , C12N9/88
CPC分类号： C12P19/56 , A23K20/10 , A23K20/121 , A23K20/163 , A23L2/60 , A23L27/36 , A23V2002/00 , C07H15/256 , C12N9/0073 , C12N9/1051 , C12N9/88 , C12N9/90 , C12N15/52 , C12P5/007 , C12Y114/13078 , C12Y204/01035 , C12Y402/03019 , C12Y505/01013
摘要： The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.

20. 发明授权

EP2173881B1 ACETYL-COA PRODUCING ENZYMES IN YEAST 有权转让
标题翻译：乙酰CoA生产酶在酵母
公开(公告)号：EP2173881B1
公开(公告)日：2016-09-28
申请号：EP08775033.7
申请日：2008-07-11
申请人： DSM IP Assets B.V.
发明人： MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine , WINKLER, Aaron Adriaan
IPC分类号： C12P7/16 , C12Q1/32 , C07K14/33 , C12N9/02
CPC分类号： C12Q1/32 , C07K14/33 , C12N9/001 , C12P7/16 , Y02E50/10

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