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    • 16. 发明申请
    • Recombinant escherichia coli and overproduction method of polyhydroxybutyrate using the strain
    • 重组大肠杆菌和使用该菌株的多羟基丁酸盐的过量产生方法
    • US20050227340A1
    • 2005-10-13
    • US10998473
    • 2004-11-29
    • In KimIl JungHyo ParkKug KimByung Choi
    • In KimIl JungHyo ParkKug KimByung Choi
    • H04N7/24C12N1/21C12N9/16C12N15/09C12P7/62C12R1/19
    • C12P7/62
    • The present invention relates to a recombinant Escherichia coli having phbCAB originated from Alcaligenes eutrophus and an overproduction method of polyhydroxyalkanoate using the same, more precisely, a recombinant Escherichia coli ‘MG1655/pTZ18U-PHB’ or ‘JIL938/pTZ18U-PHB’ having phbCAB originated from Alcaligenes eutrophus and an overproduction method of polyhydroxyalkanoate comprising the steps of preparing transformed Escherichia coli having phbCAB originated from Alcaligenes eutrophus, inoculating and culturing the cells (growth phase), inducing the production of polyhydroxyalkanoate in the recomibnant Escherichia coli (stationary phase and producing phase), and inducing the extracellular secretion of the polyhydroxyalkanoate from the recombinant Escherichia coli. The method of the present invention facilitates not only overproduction of polyhydroxyalkanoate such as polyhydroxybutyrate by a simple batch culture but also industrial use of biodegradable polyhydroxyalkanoate replacing conventional non-biodegradable plastics by its simple and easy fermentation, separation and purification process.
    • 本发明涉及具有源自嗜碱性杆菌的phbCAB的重组大肠杆菌,以及使用该重组大肠杆菌的具有phbCAB的重组大肠杆菌MG1655 / pTZ18U-PHB'或'JIL938 / pTZ18U-PHB'的多羟基链烷酸酯的过量产生方法 来自产碱杆菌属的富含嗜碱性细菌的产生方法和多羟基链烷酸酯的过量产生方法,包括以下步骤:制备具有源自产碱杆菌属细菌的phbCAB的转化大肠杆菌,接种和培养细胞(生长期),诱导在大肠杆菌(稳定期和生产阶段)中产生聚羟基链烷酸酯 ),并诱导来自重组大肠杆菌的聚羟基链烷酸酯的细胞外分泌。 本发明的方法不仅通过简单的分批培养而不仅过度生产聚羟基链烷酸酯如聚羟基丁酸酯,而且还通过其简单且容易的发酵,分离和纯化方法替代常规的不可生物降解的塑料来生产可降解的聚羟基链烷酸酯的工业应用。
    • 18. 发明申请
    • Three-dimensional printing prototyping system
    • 三维打印原型系统
    • US20060099287A1
    • 2006-05-11
    • US11093424
    • 2005-03-30
    • Dong KimTaik LeeWon LeeHyun LimByung Choi
    • Dong KimTaik LeeWon LeeHyun LimByung Choi
    • B29C35/08
    • B29C64/165B33Y30/00B33Y50/02
    • The present invention relates to a three-dimensional printing prototyping system which includes a prototyping chamber having a prototyping table capable of moving up and down by a predetermined range, a material containing chamber containing a powder material and having a material supply table capable of moving up and down by a predetermined range, and a material supplying means for supplying the powder material contained in the material containing chamber to the prototyping table as much as a sectional thickness corresponding to divided section data of a three-dimensional prototype to be prototyped. The system of the present invention comprises a printing head for injecting a photocuring adhesive material to a region, corresponding to the sectional data, on the powder material supplied to the prototyping table; a curing device for photocuring at least the region on which the adhesive material is injected; and a control computer for controlling the printing head and the curing device such that the adhesive material is injected and cured, respectively, on the region corresponding to the divided section data of the three-dimensional prototype. According to the present invention, a period of time required in curing a three-dimensional prototype can be remarkably shortened and the three-dimensional prototype with its superior solidity can be obtained.
    • 本发明涉及一种三维打印原型系统,其包括具有能够上下移动预定范围的原型台的原型制造室,包含粉末材料的材料容纳室,并且具有能够向上移动的材料供给台 并且下降预定范围,以及材料供应装置,用于将包含在材料容纳室中的粉末材料提供给原型台,多达与要制成原型的三维原型的分割部分数据相对应的截面厚度。 本发明的系统包括一个用于将光固化粘合剂材料注射到提供给原型台的粉末材料上的与分段数据对应的区域的打印头; 用于至少在其上注入粘合剂材料的区域光固化的固化装置; 以及用于控制打印头和固化装置的控制计算机,使得粘合剂材料分别在对应于三维原型的分割部分数据的区域上被注入和固化。 根据本发明,可以显着缩短三维原型的固化所需的时间,并且可以获得其具有优异的立体性的三维原型。