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11. 发明授权

US07578915B2 Multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis 失效
标题翻译：用于高通量制备分离的多通道微量系统，具有全面的收集和分析
公开(公告)号：US07578915B2
公开(公告)日：2009-08-25
申请号：US10652773
申请日：2003-08-29
申请人： Barry L. Karger , Lev Kotler , Frantisek Foret , Marek Minarik , Karel Kleparnik
发明人： Barry L. Karger , Lev Kotler , Frantisek Foret , Marek Minarik , Karel Kleparnik
IPC分类号： G01N27/447 , B01D57/02
CPC分类号： G01N30/80 , B01D15/08 , G01N27/44717 , G01N27/44782 , G01N27/44791 , G01N30/466 , G01N30/82
摘要： A modular multiple lane or capillary electrophoresis (chromatography) system that permits automated parallel separation and comprehensive collection of all fractions from samples in all lanes or columns, with the option of further on-line automated sample fraction analysis, is disclosed. Preferably, fractions are collected in a multi-well fraction collection unit, or plate. The multi-well collection plate is preferably made of a solvent permeable gel, most preferably a hydrophilic, polymeric gel such as agarose or cross-linked polyacrylamide.
摘要翻译：公开了一种模块化多通道或毛细管电泳（色谱）系统，其允许在所有泳道或色谱柱中的样品自动平行分离和全面收集所有级分，并进一步在线自动化样品分数分析。优选地，将馏分收集在多孔馏分收集单元或板中。多孔收集板优选由溶剂可渗透的凝胶制成，最优选由亲水性聚合物凝胶如琼脂糖或交联聚丙烯酰胺制成。

12. 发明授权

US06674070B2 On-line and off-line deposition of liquid samples for matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectroscopy 失效
标题翻译：用于基质辅助激光解吸电离 - 飞行时间（MALDI-TOF）质谱的液体样品的在线和离线沉积
公开(公告)号：US06674070B2
公开(公告)日：2004-01-06
申请号：US09757079
申请日：2001-01-09
申请人： Barry L. Karger , Frantisek Foret , Jan Preisler
发明人： Barry L. Karger , Frantisek Foret , Jan Preisler
IPC分类号： H01J4900
CPC分类号： H01J49/0431 , G01N1/2214 , H01J49/0418
摘要： A universal interface for continuous on-line liquid sample introduction directly to the time-of-flight mass spectrometer, which can further promote throughput and utility of MALDI-TOF MS, is disclosed. Preferably, the liquid sample includes a matrix, either solid or liquid, for use in matrix-assisted-laser-desorption-ionization, most particularly in a time-of-flight mass spectrometer which can further promote throughput and utility of MALDI-TOF MS. In the method of the invention, the same samples and matrices, both solid and liquid, can be used as in conventional MALDI. In practice of the method of the invention, a solution of sample containing, e.g., peptide and matrix is infused directly into the source chamber of a mass spectrometer at subatmospheric pressure, deposited on a moving sample holder, such as a rotating quartz wheel, and desorbed by, e.g., a nitrogen laser. The method of the invention is particularly amenable to multiplexing, the parallel deposition of multiple samples, e.g., from a capillary array or microchip channels, with subsequent sequential desorption with a scanning laser. This format is particularly useful for high throughput MS analysis. Also disclosed is an off-line deposition chamber and a general method of preparing a sample for analysis that results in the homogeneous deposition of small quantities of sample at improved reproducibility. This format of sample preparation is particularly useful with existing commercial mass spectrometers.
摘要翻译：公开了用于连续在线液体样品直接引入飞行时间质谱仪的通用界面，其可进一步促进MALDI-TOF MS的产量和效用。优选地，液体样品包括用于基质辅助激光解吸电离的固体或液体基质，最特别是在飞行时间质谱仪中，其可以进一步促进MALDI-TOF MS的通过量和效用。在本发明的方法中，可以像常规的MALDI一样使用固体和液体两种相同的样品和基质。在本发明方法的实践中，将含有例如肽和基质的样品溶液直接注入质谱仪的源室中，沉积在移动的样品架上，例如旋转的石英砂轮，以及由氮激光器解吸。本发明的方法特别适合多路复用，例如来自毛细管阵列或微芯片通道的多个样品的平行沉积，随后用扫描激光随后的顺序解吸。此格式对于高通量MS分析特别有用。还公开了离线沉积室和制备用于分析的样品的一般方法，其导致少量样品以改进的再现性均匀沉积。样品制备的这种格式对于现有的商业质谱仪是特别有用的。

13. 发明授权

US6068749A Subatmospheric, variable pressure sample delivery chamber for electrospray ionization/mass spectrometry and other applications 失效
标题翻译：低气压，可变压力样品输送室，用于电喷雾离子化/质谱和其他应用
公开(公告)号：US6068749A
公开(公告)日：2000-05-30
申请号：US784400
申请日：1997-01-17
申请人： Barry L. Karger , Frantisek Foret , Daniel P. Kirby
发明人： Barry L. Karger , Frantisek Foret , Daniel P. Kirby
IPC分类号： G01N27/447 , G01N30/28 , G01N30/72 , H01J49/04 , G01N27/26
CPC分类号： H01J49/045 , G01N27/44717 , H01J49/165 , G01N2030/285 , G01N30/7266
摘要： A subatmospheric, variable pressure sample delivery chamber, useful as an electrospray ionization device for introducing a sample into a mass spectrometer or as a sample delivery device for delivery of a sample to a collection device at subatmospheric pressures, is disclosed. The sample delivery chamber is configured to maintain an operating pressure between 1 and 750 Torr, preferably between 50 and 700 Torr. The chamber has an inlet port for introduction of a gas and an exit port capable of being coupled either to a sampling orifice for an analytical device, usually a mass spectrometer, or directly to a pump for removal of gas. A sample delivery device extends from outside the chamber into the interior of the chamber. The inlet end of the sample delivery device is capable of receiving a sample and the exit end of the sample delivery device is aligned with the exit port of the chamber, if the sample is to be delivered to an external analytical device, or aligned with the collection device, for direct collection of the sample.
摘要翻译：公开了一种低于大气压的可变压力样品输送室，其用作将样品引入质谱仪或用于将样品递送至低于大气压的收集装置的样品输送装置的电喷射离子化装置。样品输送室被配置为将操作压力保持在1至750托之间，优选在50至700托之间。腔室具有用于引入气体和出口的入口端口，该出口能够耦合到用于分析装置（通常是质谱仪）的采样孔，或者直接连接到用于去除气体的泵。样品输送装置从腔室外部延伸到腔室的内部。样品输送装置的入口端能够接收样品，并且样品输送装置的出口端与腔室的出口对准，如果样品要被输送到外部分析装置，或者与收集装置，用于直接收集样品。

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