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1. 发明授权

US06175112B1 On-line liquid sample deposition interface for matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectroscopy 失效
标题翻译：在线液体样品沉积界面，用于基质辅助激光解吸电离 - 飞行时间（MALDI-TOF）质谱
公开(公告)号：US06175112B1
公开(公告)日：2001-01-16
申请号：US09083815
申请日：1998-05-22
申请人： Barry L. Karger , Frantisek Foret , Jan Preisler
发明人： Barry L. Karger , Frantisek Foret , Jan Preisler
IPC分类号： H01J4900
CPC分类号： H01J49/0431 , H01J49/0418
摘要： A universal interface for continuous on-line liquid sample introduction directly to the time-of-flight mass spectrometer, which can further promote throughput and utility of MALDI-TOF MS, is disclosed. Preferably, the liquid sample includes a matrix, either solid or liquid, for use in matrix-assisted-laser-desorption-ionization, most particularly in a time-of-flight mass spectrometer which can further promote throughput and utility of MALDI-TOF MS. In the method of the invention, the same samples and matrices, both solid and liquid, can be used as in conventional MALDI. In practice of the method of the invention, a solution of sample containing, e.g., peptide and matrix is infused directly into the source chamber of a mass spectrometer at subatmospheric pressure, deposited on a moving sample holder, such as a rotating quartz wheel, and desorbed by, e.g., a nitrogen laser. The method of the invention is particularly amenable to multiplexing, the parallel deposition of multiple samples, e.g., from a capillary array or microchip channels, with subsequent sequential desorption with a scanning laser. This format is particularly useful for high throughput MS analysis.
摘要翻译：公开了用于连续在线液体样品直接引入飞行时间质谱仪的通用界面，其可进一步促进MALDI-TOF MS的产量和效用。优选地，液体样品包括用于基质辅助激光解吸电离的固体或液体基质，最特别是在飞行时间质谱仪中，其可以进一步促进MALDI-TOF MS的通过量和效用。在本发明的方法中，可以像常规的MALDI一样使用固体和液体两种相同的样品和基质。在本发明方法的实践中，将含有例如肽和基质的样品溶液直接注入质谱仪的源室中，沉积在移动的样品架上，例如旋转的石英砂轮，以及由氮激光器解吸。本发明的方法特别适合多路复用，例如来自毛细管阵列或微芯片通道的多个样品的平行沉积，随后用扫描激光随后的顺序解吸。此格式对于高通量MS分析特别有用。

2. 发明授权

US06825463B2 On-line and off-line deposition of liquid samples for matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectroscopy 失效
标题翻译：用于基质辅助激光解吸电离 - 飞行时间（MALDI-TOF）质谱的液体样品的在线和离线沉积
公开(公告)号：US06825463B2
公开(公告)日：2004-11-30
申请号：US10132064
申请日：2002-04-25
申请人： Barry L. Karger , Frantisek Foret , Jan Preisler
发明人： Barry L. Karger , Frantisek Foret , Jan Preisler
IPC分类号： H01J4900
CPC分类号： H01J49/0418 , G01N1/2214 , H01J49/0431
摘要： A universal interface for continuous on-line liquid sample introduction directly to the time-of-flight mass spectrometer, which can further promote throughput and utility of MALDI-TOF MS, is disclosed. Preferably, the liquid sample includes a matrix, either solid or liquid, for use in matrix-assisted-laser-desorption-ionization, most particularly in a time-of-flight mass spectrometer which can further promote throughput and utility of MALDI-TOF MS. In the method of the invention, the same samples and matrices, both solid and liquid, can be used as in conventional MALDI. In practice of the method of the invention, a solution of sample containing, e.g., peptide and matrix is infused directly into the source chamber of a mass spectrometer at subatmospheric pressure, deposited on a moving sample holder, such as a rotating quartz wheel, and desorbed by, e.g., a nitrogen laser. The method of the invention is particularly amenable to multiplexing, the parallel deposition of multiple samples, e.g., from a capillary array or microchip channels, with subsequent sequential desorption with a scanning laser. This format is particularly useful for high throughput MS analysis. Also disclosed is an off-line deposition chamber and a general method of preparing a sample for analysis that results in the homogeneous deposition of small quantities of sample at improved reproducibility on any appropriately configured sample receptor. This format of sample preparation is particularly useful with existing commercial mass spectrometers.
摘要翻译：公开了用于连续在线液体样品直接引入飞行时间质谱仪的通用界面，其可进一步促进MALDI-TOF MS的产量和效用。优选地，液体样品包括用于基质辅助激光解吸电离的固体或液体基质，最特别是在飞行时间质谱仪中，其可以进一步促进MALDI-TOF MS的通过量和效用。在本发明的方法中，可以像常规的MALDI一样使用固体和液体两种相同的样品和基质。在本发明方法的实践中，将含有例如肽和基质的样品溶液直接注入质谱仪的源室中，沉积在移动的样品架上，例如旋转的石英砂轮，以及由氮激光器解吸。本发明的方法特别适合多路复用，例如来自毛细管阵列或微芯片通道的多个样品的平行沉积，随后用扫描激光随后的顺序解吸。此格式对于高通量MS分析特别有用。还公开了离线沉积室和制备用于分析的样品的一般方法，其导致在任何适当配置的样品受体上以改善的再现性均匀沉积少量样品。样品制备的这种格式对于现有的商业质谱仪是特别有用的。

3. 发明授权

US06674070B2 On-line and off-line deposition of liquid samples for matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectroscopy 失效
标题翻译：用于基质辅助激光解吸电离 - 飞行时间（MALDI-TOF）质谱的液体样品的在线和离线沉积
公开(公告)号：US06674070B2
公开(公告)日：2004-01-06
申请号：US09757079
申请日：2001-01-09
申请人： Barry L. Karger , Frantisek Foret , Jan Preisler
发明人： Barry L. Karger , Frantisek Foret , Jan Preisler
IPC分类号： H01J4900
CPC分类号： H01J49/0431 , G01N1/2214 , H01J49/0418
摘要： A universal interface for continuous on-line liquid sample introduction directly to the time-of-flight mass spectrometer, which can further promote throughput and utility of MALDI-TOF MS, is disclosed. Preferably, the liquid sample includes a matrix, either solid or liquid, for use in matrix-assisted-laser-desorption-ionization, most particularly in a time-of-flight mass spectrometer which can further promote throughput and utility of MALDI-TOF MS. In the method of the invention, the same samples and matrices, both solid and liquid, can be used as in conventional MALDI. In practice of the method of the invention, a solution of sample containing, e.g., peptide and matrix is infused directly into the source chamber of a mass spectrometer at subatmospheric pressure, deposited on a moving sample holder, such as a rotating quartz wheel, and desorbed by, e.g., a nitrogen laser. The method of the invention is particularly amenable to multiplexing, the parallel deposition of multiple samples, e.g., from a capillary array or microchip channels, with subsequent sequential desorption with a scanning laser. This format is particularly useful for high throughput MS analysis. Also disclosed is an off-line deposition chamber and a general method of preparing a sample for analysis that results in the homogeneous deposition of small quantities of sample at improved reproducibility. This format of sample preparation is particularly useful with existing commercial mass spectrometers.
摘要翻译：公开了用于连续在线液体样品直接引入飞行时间质谱仪的通用界面，其可进一步促进MALDI-TOF MS的产量和效用。优选地，液体样品包括用于基质辅助激光解吸电离的固体或液体基质，最特别是在飞行时间质谱仪中，其可以进一步促进MALDI-TOF MS的通过量和效用。在本发明的方法中，可以像常规的MALDI一样使用固体和液体两种相同的样品和基质。在本发明方法的实践中，将含有例如肽和基质的样品溶液直接注入质谱仪的源室中，沉积在移动的样品架上，例如旋转的石英砂轮，以及由氮激光器解吸。本发明的方法特别适合多路复用，例如来自毛细管阵列或微芯片通道的多个样品的平行沉积，随后用扫描激光随后的顺序解吸。此格式对于高通量MS分析特别有用。还公开了离线沉积室和制备用于分析的样品的一般方法，其导致少量样品以改进的再现性均匀沉积。样品制备的这种格式对于现有的商业质谱仪是特别有用的。

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