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    • 20. 发明公开
    • 스트렙토마이세스 알부스 용균 추출물을 포함하는 백신 보좌제
    • 免疫抑制剂包括ALBUS溶液提取物
    • KR1020090013599A
    • 2009-02-05
    • KR1020070077854
    • 2007-08-02
    • 대한민국(농림축산식품부 농림축산검역본부장)
    • 김종만김재명이희수장금찬박신영조윤상이명헌윤소미박중원지순옥정석찬
    • A61K39/02A61P37/02A61K39/085
    • A61K39/05C12R1/47Y10S435/887Y10S514/885
    • An immunostimulant containing the lystic extract of Streptomyces albus is provided to reinforce the functions of various immune cells having the major role in the disease defense, thereby increasing the specific and nonspecific resistance to the disease infection of animals. The immunostimulant contains the lystic extract of Streptomyces albus which is prepared by cultivating Streptomyces albus in yeast malt extract agar broth at 26 deg. C for 72 hours, centrifuging the cultured fluid at 3000Xg for 30 minutes and collecting it, rinsing the collected pellet with phosphate buffered saline(PBS) of pH 7.2 and suspending the rinsed pellet in PBS, adding 5% of TNT solution containing 50mM of Tris-HCl, 0.15M of NaCl and 4% Triton x-114 as solubilizing agent into the Streptomyces albus suspension, stirring the mixture at room temperature for 2-3 days to solubilize the Streptomyces albus, and regulating pH of the solution to 6.5-7.2, and drying the solution in the thermostatic chamber.
    • 提供含有链霉菌(Streptomyces albus)的提取物的免疫刺激剂,以增强在疾病防御中具有主要作用的各种免疫细胞的功能,从而增加对动物疾病感染的特异性和非特异性抗性。 免疫刺激剂含有通过在26度的酵母麦芽提取物琼脂培养基中培养链霉菌(Streptomyces albus)而制备的链霉菌链霉菌的提取物。 培养72小时,将培养液以3000Xg离心30分钟并收集,用pH7.2的磷酸盐缓冲盐水(PBS)冲洗收集的沉淀物,并将漂洗的沉淀悬浮于PBS中,加入5%含有50mM Tris -HCl,0.15M NaCl和4%Triton x-114作为增溶剂加入到Streptomyces albus悬浮液中,在室温下搅拌混合物2-3天以溶解链霉菌,并将溶液的pH调节至6.5-7.2 ,并干燥恒温室中的溶液。