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1. 发明公开

KR1020140000810A 분변 중 요네균 신속분리를 위한 자석 나노입자를 이용한 검출방법 有权
标题翻译：免疫测定方法检测MYCOBACTERIUM AVIUM SUBSP。 FECAL样品中的PARATUBERCULOSIS
公开(公告)号：KR1020140000810A
公开(公告)日：2014-01-06
申请号：KR1020120068128
申请日：2012-06-25
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 김재명 , 구복경 , 강환구 , 김나래 , 이햇님 , 이혁미 , 정석찬
IPC分类号： C12Q1/68 , G01N33/569 , C12Q1/04
CPC分类号： G01N33/5695 , C12Q1/04 , C12Q1/686
摘要： The present invention relates to a detection method using magnetic nanoparticles for rapidly isolating Mycobacterium avium subsp. paratuberculosis from fecal samples and, more specifically, to a method for detecting the Mycobacterium avium subsp. paratuberculosis which isolates the Mycobacterium avium subsp. paratuberculosis with high sensitivity which can be existed in animal fecal samples using the magnetic nanoparticles to which Mycobacterium avium subsp. paratuberculosis specific antibodies are combined and rapidly and simply checks infection of the Mycobacterium avium subsp. paratuberculosis by performing polymerase chain reaction using primers which are specifically combined to DNA of the Mycobacterium avium subsp. paratuberculosis. According to the present invention, the detection method using the magnetic nanoparticles for rapidly isolating the Mycobacterium avium subsp. paratuberculosis from fecal samples can easily detect the Mycobacterium avium subsp. paratuberculosis even if small number of the Mycobacterium avium subsp. paratuberculosis is existed in biological samples such as feces and prevent contagion to different entities by isolating the infected entities from an allied army by performing the method simply and rapidly.
摘要翻译：本发明涉及使用磁性纳米颗粒快速分离鸟分枝杆菌亚种的检测方法。来自粪便样品的副结核病，更具体地，涉及用于检测鸟分枝杆菌亚种的方法。分离鸟分枝杆菌亚种的副结核病具有高灵敏度的副结核病，其可以存在于动物粪便样品中，使用鸟分枝杆菌亚种的磁性纳米颗粒。组合结核分枝杆菌特异性抗体并快速简单地检查鸟分枝杆菌感染。使用与鸟分枝杆菌亚种DNA的特异性组合的引物进行聚合酶链反应，来治疗副结核病。副结核病。根据本发明，使用磁性纳米颗粒快速分离鸟分枝杆菌亚种的检测方法。来自粪便样本的副结核病可以很容易地检测到鸟分枝杆菌。副结核病即使少数分枝杆菌分枝杆菌。副结核病存在于粪便等生物样本中，通过简单，快速地进行方法，将受感染的实体与盟军分离，防止对不同实体的传染。

2. 发明公开

KR1020090117448A 연교추출물을 이용한 사료 첨가용 항산화제 无效
标题翻译：抗氧化剂，用于加入牛肉提取物
公开(公告)号：KR1020090117448A
公开(公告)日：2009-11-12
申请号：KR1020080043496
申请日：2008-05-09
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 이희수 , 임숙경 , 박신영 , 김재명 , 김종만 , 임춘태 , 정석찬 , 장금찬 , 시앙슈피아오
IPC分类号： A23K1/17 , A23K1/14 , A23K1/16 , A23K1/00
CPC分类号： A23K10/30 , A23K20/195
摘要： PURPOSE: An antioxidant for supplementing feed using Forsythiae fructus extract and a preparation method for the Forsythiae Fructus extract are provided to show improved daily gain, feed intake, and feed efficiency compared to a control group. CONSTITUTION: An antioxidant for supplementing feed contains Forsythiae fructus extract. A method for preparing Forsythiae fructus extract comprises the following steps of: washing 100g Forsythiae fructus with water to remove dust; placing the washed Forsythiae fructus in a white bag; adding 1,000ml distilled water into the white bag to soak for 20-30 minutes; boiling down the soaked Forsythiae fructus for 150 minutes and obtaining an extract; adding another 800ml distilled water and boiling down for 150 minutes to obtain the extract; adding another 700ml distilled water and boiling down for 150 minutes to obtain the extract; collecting the extracts and concentrating until the volume becomes 100ml; and freeze-drying the concentrate.
摘要翻译：目的：提供使用连翘果提取物补充饲料的抗氧化剂和连翘果提取物的制备方法，以显示与对照组相比改善的日增重，进食量和饲料效率。构成：补充饲料的抗氧化剂含有连翘果提取物。连翘果提取物的制备方法为：用水洗涤连翘100份，除去灰尘; 将洗涤的连翘属植物置于白色袋中; 在白色袋中加入1000ml蒸馏水浸泡20-30分钟; 煮熟连翘150分钟，得到提取物; 加入800ml蒸馏水，煮沸150分钟，得到提取物; 再加入700ml蒸馏水，煮沸150分钟，得到提取物; 收集提取物并浓缩直到体积变成100ml; 并冷冻干燥浓缩物。

3. 发明公开

KR1020090093292A 비특이 면역증강 효능이 있는 싸카로마이세스 체바리에리용균 추출물 无效
标题翻译：用于改善非特异性免疫球蛋白的细菌提取物
公开(公告)号：KR1020090093292A
公开(公告)日：2009-09-02
申请号：KR1020080018738
申请日：2008-02-29
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 김종만 , 김재명 , 이희수 , 장금찬 , 남향미 , 조윤상 , 이명헌 , 윤소미 , 박중원 , 정석찬
IPC分类号： A61K36/06 , A23K1/16
CPC分类号： A61K36/06
摘要： A bacteriolysis extract of Saccharomyces chevalieri is provided to increase the activation of neutrophil and enhance non-specific defense activity against the pathogenic bacteria. An immune enhancer, vaccine adjuvant, adjuvant treating agent or feed additive comprises bacteriolysis extract of Saccharomyces chevalieri as an active ingredient. A method for manufacturing a bacteriolysis extract of Saccharomyces chevalieri comprises: a step of culturing Saccharomyces chevalieri in media at 26°C for 72 hours; a step of centrifuging media and suspending in PBS; a step of adding dissolution agent and treating at room temperature for 2-3 days; a step of confirming the dissolution status with a microscope; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a refrigerator.
摘要翻译：提供雪花酵母的溶菌提取物以增加嗜中性粒细胞的活化并增强针对致病菌的非特异性防御活性。免疫增强剂，疫苗佐剂，佐剂处理剂或饲料添加剂包括作为活性成分的雪花酵母的溶菌提取物。酿酒酵母的溶菌提取物的制备方法包括：在培养基中在26℃下培养雪花酵母（Saccharomyces chevalieri）72小时的步骤; 离心培养基并悬浮于PBS中的步骤; 加入溶解剂并在室温下处理2-3天的步骤; 用显微镜确认溶出状态的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在冰箱中干燥和保持的步骤。

4. 发明公开

KR1020090091884A 비특이 면역증강 효능이 있는 자이고사카로마이세스 바일리용균추출물 有权
标题翻译：具有改善非特异性免疫应答效果的ZYGOSACCHAROMYCES BAILII的LYSATE
公开(公告)号：KR1020090091884A
公开(公告)日：2009-08-31
申请号：KR1020080017089
申请日：2008-02-26
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 김종만 , 김재명 , 이희수 , 장금찬 , 남향미 , 조윤상 , 이명헌 , 윤소미 , 박중원 , 정석찬
IPC分类号： A61K36/06 , A23K1/16
CPC分类号： A61K36/06
摘要： An immune enhancer and vaccine adjuvant additive for animal, which contains Zygosaccharomyces bailii lysates and an auxiliary therapeutic agent are provided to enhance the activation of neutrophil and non-specific protection effect to attack inoculation. An immune enhancer, vaccine adjuvant additive, auxiliary therapeutic agent and feed additive comprises a Zygosaccharomyces bailii lysates. A method for manufacturing the Zygosaccharomyces bailii lysate comprises: a step of culturing the Zygosaccharomyces bailii in the media at 26‹C for 72 hours; a step of centrifuging the cultured media and suspending in phosphate buffered saline (PBS); a step of adding dissolution agent and stirring at room temperature for two to three days; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a thermostatic chamber.
摘要翻译：提供含有接合酵母发酵酵母裂解物和辅助治疗剂的动物免疫增强剂和疫苗佐剂添加剂，以增强中性粒细胞的活化和非特异性保护作用来攻击接种。免疫增强剂，疫苗佐剂添加剂，辅助治疗剂和饲料添加剂包括接合酵母（Zygosaccharomyces bailii lysates）。一种制造接合酵母发酵酵母裂解物的方法，包括：在培养基中培养接合酵母属酵母在26℃72小时的步骤; 将培养基离心并悬浮于磷酸盐缓冲盐水（PBS）中的步骤; 在室温下加入溶解剂并搅拌2〜3天的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在恒温室中干燥和保持的步骤。

5. 发明公开

KR1020090021611A 동물의 면역능을 증강시키는 노란 민들레 열탕 추출물, 그제법 및 용도 无效
标题翻译：用于改善动物免疫力的UM UM AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME
公开(公告)号：KR1020090021611A
公开(公告)日：2009-03-04
申请号：KR1020070086251
申请日：2007-08-27
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 김종만 , 김재명 , 이희수 , 장금찬 , 박신영 , 조윤상 , 이명헌 , 윤소미 , 박중원 , 지순옥 , 정석찬
IPC分类号： A61K36/288 , A61P37/02
摘要： Provided is an immunostimulant using dandelion extract which improves a function of a nonspecific defense against pathogen and increases activity of animal immunocytes such as neutrophil and macrophages. An immunostimulant using dandelion extract comprises dandelion extract obtained by a hot water process as an active ingredient. A method for preparing the immunostimulant comprises a steps of extracting the dandelion extract at a temperature of 90~100°C by hot distilled water. The dandelion extract can be used independently or with additives which are generally used.
摘要翻译：提供使用蒲公英提取物的免疫刺激剂，其改善对病原体的非特异性防御的功能并增加动物免疫细胞如嗜中性粒细胞和巨噬细胞的活性。使用蒲公英提取物的免疫刺激剂包含通过热水法获得的蒲公英提取物作为活性成分。制备免疫刺激剂的方法包括以下步骤：通过热蒸馏水在90〜100℃的温度下提取蒲公英提取物。蒲公英提取物可以单独使用或与通常使用的添加剂一起使用。

6. 发明公开

KR1020080081499A 동물의 면역능을 증강시키는 싸카로마이세스 속 분비다당체및 그 제조방법 无效
标题翻译：多糖提取动物疫苗的免疫力及其制备方法
公开(公告)号：KR1020080081499A
公开(公告)日：2008-09-10
申请号：KR1020070021570
申请日：2007-03-05
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 김종만 , 김재명 , 장금찬 , 정석찬
IPC分类号： A61K47/36 , A61P37/02
CPC分类号： A61K36/064 , C12N1/18
摘要： An exo-polysaccharide derived from a Saccharomyces spp. strain is provided to enhance various activities of neutrophil, macrophage and lymphocyte, thereby being widely applied to an animal immunity-enhancing agent, a vaccine supplemental additive and an adjuvant therapeutic agent. An animal immunity enhancing agent comprises exo-polysaccharides extracted from a culture material of a Saccharomyces spp. strain. A method for preparing the exo-polysaccharides derived from the Saccharomyces spp. strain comprises the steps of: (a) after suspending pure colonies obtained by culturing the Saccharomyces spp. strain in sterile saline solution, inoculating 1 ml of the suspension into 1,000 ml of yeast malt broth and culturing it at a temperature of 28 deg.C for 72 hours; (b) after adding two times amount of distilled water into a culture material obtained from the step(a) and mixing it, boiling the mixture at a temperature of 100 deg.C for 1 hour and centrifuging the boiled mixture under 5,000xg for 30 minutes to collect supernatant therefrom; and (c) after adding two times amount of ethyl alcohol to the supernatant and mixing it, leaving a mixture in a cool chamber at a temperature of 4 deg.C for 2 days and collecting precipitated polysaccharides therefrom.
摘要翻译：衍生自酵母属（Saccharomyces spp。）的外多糖。提供菌株以增强嗜中性粒细胞，巨噬细胞和淋巴细胞的各种活性，从而广泛应用于动物免疫增强剂，疫苗补充添加剂和辅助治疗剂。动物免疫增强剂包括从酵母属的培养物质中提取的外多糖。应变。一种制备衍生自酵母属（Saccharomyces spp）的外多糖的方法。菌株包括以下步骤：（a）悬浮通过培养酵母属（Saccharomyces spp）获得的纯菌落后。在无菌生理盐水溶液中，将1ml悬浮液接种到1,000ml酵母麦芽培养液中，并在28℃的温度下培养72小时; （b）在向步骤（a）获得的培养物中加入两倍量的蒸馏水并混合后，在100℃的温度下将混合物煮沸1小时，并将煮沸的混合物在5,000xg下离心30分钟分钟收集上清液; 和（c）在向上清液中加入两倍量的乙醇并混合后，在4℃的温度下在冷室中留下混合物2天，并从中收集沉淀的多糖。

7. 发明公开

KR1020080068361A 평판응집반응기를 이용한 면역반응 측정방법 有权
标题翻译：新型平板电泳测试仪及其使用方法确定了AGAGUTINATION反应的强度
公开(公告)号：KR1020080068361A
公开(公告)日：2008-07-23
申请号：KR1020070006055
申请日：2007-01-19
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 조동희 , 김종완 , 남향미 , 허은정 , 조윤상 , 황인영 , 김재명 , 김종만 , 정석찬
IPC分类号： G01N33/53 , G01N33/50 , G01N33/48
摘要： A plate agglutination tester and a method for measuring an immuno-reaction using the same are provided to decrease error of the result analysis in accordance with proficiency of a person performing diagnosis, show the degree of various immuno-reactions in an objective value and perform various statistical analyses, thereby being very useful for measuring the immuno-reaction. A plate agglutination tester consists of a transparent film and is characterized in that a section, which consists of a plurality of circles and where an antigen-antibody agglutination reaction occurs, is arranged and formed in horizontal and vertical directions on an upper side of the film. A method for measuring an immuno-reaction comprises the steps of: (a) mixing an antigen and an antibody specifically bound to the antigen with the plate agglutination tester to induce an agglutination reaction; (b) scanning the agglutination reaction induced plate agglutination tester and converting a scanning result into an image file; and (c) analyzing the obtained image file using a computer program capable of detecting fine agglutination reactants to numberize the intensity of the agglutination reaction. Further, the transparent film is an over head project film.
摘要翻译：提供平板凝集试验机和使用其的免疫反应测定方法，根据进行诊断的人员的熟练程度来减少结果分析的误差，以目标值显示各种免疫反应的程度，并进行各种统计分析，因此对测量免疫反应非常有用。板凝集试验仪由透明膜构成，其特征在于，在膜的上侧，在水平和垂直方向上布置并形成由多个圆组成并且发生抗原 - 抗体凝集反应的部分。测定免疫反应的方法包括以下步骤：（a）将抗原与抗原特异性结合的抗体与平板凝集试验仪混合以诱导凝集反应; （b）扫描凝集反应诱导的板凝集测试仪并将扫描结果转换为图像文件; 和（c）使用能够检测细微凝集反应物的计算机程序来分析所获得的图像文件，以对凝集反应的强度进行编号。此外，透明膜是顶部投影膜。

8. 发明授权

KR101687156B1 마이코박테리움 보비스 감염 진단용 조성물 및 이를 이용한 진단방법 有权
标题翻译：用于诊断牛分枝杆菌的组合物及其诊断方法
公开(公告)号：KR101687156B1
公开(公告)日：2016-12-20
申请号：KR1020140100499
申请日：2014-08-05
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 김재명 , 장윤호 , 김나래 , 정석찬
IPC分类号： C12Q1/68
摘要： 본발명은마이코박테리움보비스감염진단용조성물, 진단용키트및 진단방법에관한것이다. 본발명에따른마이코박테리움보비스감염진단용프라이머세트를이용시, 상기마이코박테리움보비스균에대해높은특이도및 민감도로검출하는효과가있어, 마이코박테리움보비스균의신속한검출및 진단에유용하게이용될수 있다.
摘要翻译：本发明涉及用于诊断牛分枝杆菌感染的组合物，诊断试剂盒和诊断方法。根据本发明，通过使用用于诊断牛分枝杆菌感染的引物组，可以高度特异性和灵敏度地检测牛分枝杆菌，从而可以快速检测和诊断牛分枝杆菌。

9. 发明授权

KR101201420B1 신규 락토바실러스 존슨니 및 이를 포함하는 사료첨가제 조성물 有权
标题翻译：含有新型乳酸乳杆菌的饲料添加剂
公开(公告)号：KR101201420B1
公开(公告)日：2012-11-15
申请号：KR1020100070004
申请日：2010-07-20
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 김재명 , 소병재 , 최정업 , 정갑수
IPC分类号： A23K1/16 , C12N1/20
摘要： 본 발명은 내산성, 내담즙성, 장내부착능, 담즙산염가수분해능이 우수하고, 항생제에 대한 감수성이 있으며, 동물이 섭취했을 때 증체량, 병원성 세균 방어효과 등의 우수한 효과를 나타내는 신규한 락토바실러스 존슨니에 관한 것이다.
본 발명의 신규한 락토바실러스 존슨니를 동물용 사료 또는 의약품으로 사용할 경우, 동물의 장내 병원성 세균 억제 및 정장효과를 지속적으로 나타낼 수 있어 축산 농가의 가축질병으로 인한 경제적 피해를 크게 감소시킬 수 있고, 뿐만 아니라 이를 활용하여 수입 동물용 의약품을 대체할 수 있다.

10. 发明公开

KR1020110009638A 신규한 락토바실러스속 유산균 복합 균주를 포함하는 사료첨가제 有权
标题翻译：饲料添加剂新鲜乳酸杆菌SPP。复杂
公开(公告)号：KR1020110009638A
公开(公告)日：2011-01-28
申请号：KR1020100070745
申请日：2010-07-22
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 김재명 , 김종만 , 남향미 , 장금찬 , 이희수 , 정석찬
IPC分类号： A23K1/16 , C12N1/20
CPC分类号： A23K10/18 , A23K20/195 , C12N1/20 , C12R1/225
摘要： PURPOSE: A feed additive composition including novel lactobacillus species lactobacillus mixed bacterial strain is provided to offer the excellent antibacterial activity and starch resolution function for various pathogenic bacteria. CONSTITUTION: A feed additive composition contains a lactobacillus mixed bacterial strain formed with lactobacillus johnsonii G22-2, lactobacillus reuteri G8-5, and lactobacillus salivarius G1-1. The lactobacillus mixed bacterial strain has the antimicrobial activity against pathogenic bacteria, the starch degradation capability, the acid resistance, the bile tolerance, and the intestine adhesion capability.
摘要翻译：目的：提供包含新型乳酸杆菌种乳杆菌混合菌株的饲料添加剂组合物，为各种病原菌提供优异的抗菌活性和淀粉分解功能。构成：饲料添加剂组合物含有由约翰逊乳杆菌G22-2，乳酸杆菌G8-5和唾液乳杆菌G1-1形成的乳杆菌混合菌株。乳杆菌混合菌株对病原菌具有抗菌活性，淀粉降解能力，耐酸性，胆汁耐受性和肠粘连能力。

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