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11. 发明授权

US5221521A Sample liquid dilution system for analytical measurements 失效
标题翻译：用于分析测量的样品液体稀释系统
公开(公告)号：US5221521A
公开(公告)日：1993-06-22
申请号：US735911
申请日：1991-07-25
申请人： Yoshio Hashizume , Akio Kariyone , Ryuzo Hayashi
发明人： Yoshio Hashizume , Akio Kariyone , Ryuzo Hayashi
IPC分类号： G01N1/38 , G01N35/08
CPC分类号： G01N1/38 , G01N35/085 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： A liquid dilution system which dilutes a sample to be analyzed with a carrier to supply a diluted sample-containing liquid to an analytical measurement apparatus of flow type. The inventive dilution system features simplicity and versatility as compared to conventional automatic apparatus with robot concept, and the present system comprises: a first carrier pump for feeding a first carrier; a sample injection unit for injecting the sample into the first carrier; a main passage for flowing a liquid from the sample injection unit to a detector unit of an analytical measurement apparatus; a branching device located downstream of the sample injection unit for forming a branched passage to remove a liquid mass partially from the main passage; a confluence unit located downstream of the branching unit for confluencing a second carrier; and a second carrier pump for feeding the second carrier to the confluence unit. Dilution and mixing of the sample with a carrier is done twice, once at the sample injection and then again at confluence.

12. 发明授权

US5190872A Immobilized alcohol utidase for use in an alcohol measuring apparatus 失效
标题翻译：用于酒精测量仪器的稳定的酒精使用
公开(公告)号：US5190872A
公开(公告)日：1993-03-02
申请号：US573066
申请日：1990-08-27
申请人： Yoshio Hashizume , Akio Kariyone , Ryuzo Hayashi , Minako Oka
发明人： Yoshio Hashizume , Akio Kariyone , Ryuzo Hayashi , Minako Oka
IPC分类号： G01N27/327 , C12N11/00 , C12N11/06 , C12N11/14 , C12Q1/00 , G01N27/416
CPC分类号： C12N11/06 , C12Q1/003 , Y10S435/817
摘要： Alcohol oxidase is immobilized by bonding an aminosilane coupling agent to a carrier, bonding a multifunctional aldehyde such as glutaraldehyde to an amino group of the aminosilane, washing the carrier to remove free multifunctional aldehyde and bonding alcohol oxidase to the multifunctional aldehyde bonded to the aminosilane. Washing to remove free multifunctional aldehyde enables immobilizing a one molecule thickness of alcohol oxidase on the carrier since free multifunctional aldehyde is not present to cross-link alcohol oxidase molecules together. A thin layer of alcohol oxidase is advantageous when assaying for alcohol since a thin layer does not retain hydrogen peroxide that can deactivate alcohol oxidase. The carrier preferably contains hydroxyl groups and is porous, and can be a silicate-containing carrier such as diatomaceous earth or fire brick. The immobilized alcohol oxidase is used in a column as part of an apparatus for measuring alcohol or as part of an apparatus containing immobilized glucose oxidase for measuring glucose and alcohol.

13. 发明授权

US5081015A Enzyme electrode and method for determination of alcohol content using the same 失效
标题翻译：酶的电极和使用该醇的醇的测定方法
公开(公告)号：US5081015A
公开(公告)日：1992-01-14
申请号：US401132
申请日：1989-08-30
申请人： Ryuzo Hayashi , Akio Kariyone , Yoshio Hashizume
发明人： Ryuzo Hayashi , Akio Kariyone , Yoshio Hashizume
IPC分类号： C12Q1/00
CPC分类号： C12Q1/005 , Y10S435/817 , Y10S435/921 , Y10S435/938
摘要： An enzyme electrode possessing an immobilized enzyme membrane or an immobilized enzyme layer composed by applying a solution containing alcohol oxidase and a crosslinking agent, in which the solution further contains reduced glutathione.According to the invention it is possible to immobilize alcohol oxidase stably, and an excellent immobilized enzyme electrode for alcohol measurement is obtained. Furthermore, by this invention, a highly sensitive and stable measuring method is conducted quite easily.

14. 发明授权

US4523205A Heat-sensitive recording materials 失效
标题翻译：热敏记录材料
公开(公告)号：US4523205A
公开(公告)日：1985-06-11
申请号：US552919
申请日：1983-11-18
申请人： Toshitake Suzuki , Ryuzo Hayashi , Naoto Arai , Yoshitaka Oeda
发明人： Toshitake Suzuki , Ryuzo Hayashi , Naoto Arai , Yoshitaka Oeda
IPC分类号： B41M5/30 , B41M5/327 , B41M5/337 , B41M5/18
CPC分类号： B41M5/3375 , B41M5/327 , B41M5/3275
摘要： A heat-sensitive material comprising a support having formed thereon a heat-sensitive recording layer, which has a high whiteness and is suitable for use in high-speed recording, is disclosed. The heat-sensitive recording layer comprises a colorless or pale-colored basic dye and a color developing material that, when heated, reacts with the basic dye to form a color, wherein the heat-sensitive recording layer further comprises at least one heat-fusible material having a melting point in the range of 70.degree. C. to 140.degree. C. and selected from the compounds represented by following formulae (I) to (VI): ##STR1## wherein R.sub.1, R.sub.2, R.sub.5, R.sub.6, and R.sub.7 independently represent a phenyl group, a benzyl group, or a tolyl group, each of which groups may be substituted with a halogen atom; and R.sub.3 and R.sub.4 independently represent an aryl group or an aralkyl group, each of which groups may be substituted with an alkyl group, an aralkyl group, a halogen atom, a hydroxy group, an alkoxy group, an aryloxy group, an alkylcarbonyloxy group, an arylcarbonyloxy group, an alkoxycarbonyl group, or an aryloxycarbonyl group.
摘要翻译：公开了一种热敏材料，其包括其上形成有具有高白度并且适合用于高速记录的热敏记录层的支撑体。热敏记录层包括无色或浅色碱性染料和彩色显影材料，当被加热时，它们与碱性染料反应形成一种颜色，其中热敏记录层还包括至少一种热熔记录层（I）至（VI）表示的化合物选自熔点在70℃至140℃范围内的材料。（I）图像（I）（VI）其中R 1，R 2，R 5，R 6和R 7独立地表示苯基，苄基或甲苯基，其中每个基团可以被卤素原子取代; R3和R4独立地表示芳基或芳烷基，其中的每一个可以被烷基，芳烷基，卤素原子，羟基，烷氧基，芳氧基，烷基羰基氧基，芳基羰基氧基，烷氧基羰基或芳氧基羰基。

15. 发明授权

US5306413A Assay apparatus and assay method 失效
标题翻译：测定仪器和测定方法
公开(公告)号：US5306413A
公开(公告)日：1994-04-26
申请号：US37421
申请日：1993-03-26
申请人： Ryuzo Hayashi , Yukie Inoue , Akio Kariyone
发明人： Ryuzo Hayashi , Yukie Inoue , Akio Kariyone
IPC分类号： C12Q1/00 , G01N27/26
CPC分类号： C12Q1/005 , Y10S435/817
摘要： The present invention relates to a simple and yet multifunctional system for assay employing immobilized enzymes.
摘要翻译：本发明涉及使用固定化酶的简单而又多功能的测定系统。

16. 发明申请

US20120252318A1 TOOTH-PROFILE MANAGEMENT SYSTEM FOR SHAVING-CUTTER GRINDING MACHINE 有权
标题翻译：切割机研磨机的牙齿型材管理系统
公开(公告)号：US20120252318A1
公开(公告)日：2012-10-04
申请号：US13502752
申请日：2010-10-06
申请人： Yoshikoto Yanase , Ryuzo Hayashi
发明人： Yoshikoto Yanase , Ryuzo Hayashi
IPC分类号： B24B3/12
CPC分类号： B23F19/06 , B23F21/28 , B24B49/02 , B24B53/085
摘要： A personal computer has correction coefficients (α) for tooth-profile error correction and correction coefficients (β) for meshing position correction which are set for each number of times a shaving cutter is sharpened, and for each cutter feature of the shaving cutter. Target tooth-profile data (Do), tooth-profile error data (ΔD) which is the difference between the target tooth-profile data (Do) and measured tooth-profile data (Dm), and the correction coefficient (α) and the correction coefficient (β) captured in correspondence with the number of times sharpening is performed, and the cutter features are applied to an equation Dcc=Do+α·ΔD+β to find aimed tooth-profile data (Dcc). Shaving-cutter tooth-profile data (ds) is found from the aimed tooth-profile data (Dcc). Thus, the shaving cutter can be sharpened appropriately even when the outer diameter and tooth thickness of the shaving cutter are reduced by sharpening the shaving cutter.
摘要翻译：个人计算机具有用于齿廓修正的校正系数（α）和针对剃须刀被削尖的每个次数设定的啮合位置校正的校正系数（＆bgr），以及剃须刀的每个切割器特征。目标齿廓数据（Do），作为目标齿廓数据（Do）与测量齿廓数据（Dm）之间的差异的齿廓误差数据（＆Dgr; D）和校正系数（α）并且与执行锐化次数相对应地捕获的校正系数（＆bgr），并且切割器特征被应用于等式Dcc = Do +α·＆Dgr; D +＆bgr; 找到目标牙齿轮廓数据（Dcc）。从目标牙齿轮廓数据（Dcc）中可以看到剃刀齿廓数据（ds）。因此，即使通过磨削剃须刀来减少剃须刀的外径和齿厚度，也可以适当地削尖剃须刀。

17. 发明授权

US5248597A Method and apparatus for analyzing starch and related carbohydrates 失效
标题翻译：分析淀粉和相关碳水化合物的方法和装置
公开(公告)号：US5248597A
公开(公告)日：1993-09-28
申请号：US682953
申请日：1991-04-10
申请人： Ryuzo Hayashi , Yoshio Hasizume , Akio Kariyone
发明人： Ryuzo Hayashi , Yoshio Hasizume , Akio Kariyone
IPC分类号： C12M1/40 , C12Q1/00 , C12Q1/26 , C12Q1/40 , C12Q1/54 , G01N33/66
CPC分类号： G01N33/66 , C12Q1/005
摘要： A method and apparatus for the determination of the average molecular weight, average chain length or dextrose equivalent of starch and related carbohydrates containing glucose units. The starch is analyzed by determining the free glucose amount (Gf) contained in starch or related carbohydrate sample, determining a gross glucose amount (Gt) rendered by hydrolysis of starch or related carbohydrates, and further determining a glucose amount (Gr) rendered by hydrolysis of a reduced product which is obtained by reduction of reducing terminals contained in starch or related carbohydrate.The average molecular weight, average chain length or dextrose equivalent of the starch is determined using the formulas stated below from the measured amounts of the free glucose amount (Gf), and the glucose amount (Gr). The apparatus preferably utilized immobilized enzymes to conduct the hydrolysis of the starch sample, contains a device for recognizing and storing the measured values and computed data, and contains further a device for computing average molecular weight (Ma), average chain length (Dp) or dextrose equivalent value (DE) according to the following expressions: Dp=2.multidot.Gt/(Gt-Gr+Gf), Ma=18(19.multidot.Gt+Gf-Gr)/(Gt-Gr+Gf), DE=1000(Gt-Gr+Gf)/(19.multidot.Gt-Gr+Gf), or DE=1000/(9.multidot.Dp+1).

18. 发明授权

US5242793A Selective permeable membrane and electrode using the same 失效
标题翻译：选择性渗透膜和使用其的电极
公开(公告)号：US5242793A
公开(公告)日：1993-09-07
申请号：US490709
申请日：1990-03-07
申请人： Akio Kariyone , Ryuzo Hayashi , Yoshio Hashizume
发明人： Akio Kariyone , Ryuzo Hayashi , Yoshio Hashizume
IPC分类号： B01D69/14 , C12N11/02 , C12N11/10 , C12Q1/00 , G01N27/40
CPC分类号： C12Q1/003 , B01D69/141 , C12N11/02 , C12N11/10 , C12Q1/002 , G01N27/3271 , Y10S435/817
摘要： An electrode comprising an electrically conductive base, and a selectively permeable membrane which is produced by forming a membrane from a mixed solution comprising (a) albumin, (b) at least one type of crosslinking agent and (c) chitosan. The selectively permeable membrane is useful, e.g., in conjuction with an electrode. An enzyme electrode is also provided having the selectively permeable membrane disposed on the electrode and an immobilized enzyme membrane disposed on the selectively permeable membrane. The immobilized enzyme membrane is prepared by incorporating a carboxylate into the solution containing the enzyme and an aldehyde as the crosslinking agent. The crosslinking is effected by heating or drying.

19. 发明授权

US5081037A Enzyme electrode for measuring malto-oligosaccharide 失效
标题翻译：用于测量MALTO-OLIGOSACCHARIDE的酶电极
公开(公告)号：US5081037A
公开(公告)日：1992-01-14
申请号：US321165
申请日：1989-03-09
申请人： Akio Kariyone , Yoshio Hashizume , Ryuzo Hayashi
发明人： Akio Kariyone , Yoshio Hashizume , Ryuzo Hayashi
IPC分类号： G01N27/327 , C12M1/40 , C12Q1/00 , G01N27/30 , G01N27/416
CPC分类号： C12Q1/005 , Y10S435/817 , Y10S435/913 , Y10S435/939
摘要： An enzyme electrode for measuring malto-oligosaccharides having a co-immobilized enzyme membrane containing glucoamylase and a glucose oxidizing enzyme such as glucose oxidase or pyranose oxidase.The glucoamylase and glucose oxidizing enzyme are immobilized at a ratio of ##EQU1## where Va is the maximum reaction rate of glucoamylase as expressed in the glucose formation rate from maltose, and Vo is the maximum reaction rate of glucose oxidizing enzyme as expressed in the hydrogen peroxide formation rate from glucose.In such enzyme electrode, malto-oligosaccharides are detected at the response value proportional to their degree of polymerization. Therefore, the malto-oligosaccharides can be measured at the glucose converted concentration.

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