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11. 发明申请

WO2006065137A3 NOVEL EFFICIENT PRODUCTION PROCESS FOR CAPSULAR POLYSACCHARIDES OF PATHOGENIC GRAMPOSITIVE BACTERIA BY HETEROLOGOUS EXPRESSION AND SECRETION OF COMPLEX POLYSACCHARIDES IN NON-PATHOGENIC, NON-INVASIVE GRAM- POSITIVE BACTERIA 审中-公开
标题翻译：通过异源表达和复合多糖在非致病性非侵入性阳性细菌中的分泌的病原性细菌性细菌的多糖的高效生产方法
公开(公告)号：WO2006065137A3
公开(公告)日：2006-10-12
申请号：PCT/NL2005050079
申请日：2005-12-16
申请人： SCHAPPEN STICHTING TECHNOLOGIS , NIEROP GROOT MASJA NATHALIE , DE VOS WILLEM MEINDERT , KLEEREBEZEM MICHIEL
发明人： NIEROP GROOT MASJA NATHALIE , DE VOS WILLEM MEINDERT , KLEEREBEZEM MICHIEL
IPC分类号： C07K14/31 , A61K39/02 , A61K39/09 , C12N1/21 , C12N15/52 , C12N15/74
CPC分类号： C07K14/3156 , A61K39/00 , A61K39/02 , A61K39/07 , A61K39/085 , A61K39/09 , A61K39/092 , A61K2039/523
摘要： The current invention provides methods and means for heterologous expression, production and/or secretion of complex capsular polysaccharides in non- pathogenic, non-invasive Gram-positive bacteria. The invention in particular provides non- pathogenic, non- invasive Gram-positive bacteria capable of expression and/or secretion of heterologous, complex polysaccharides from a pathogenic bacterial species. Such bacteria and polysaccharides produced therein may be applied according to the invention to provide compositions for vaccination for the treatment and prevention of infectious bacterial diseases.
摘要翻译：本发明提供在非致病性，非侵入性革兰氏阳性细菌中异源表达，产生和/或分泌复合荚膜多糖的方法和手段。本发明特别提供能够从病原菌物种表达和/或分泌异源复合多糖的非致病性，非侵入性革兰氏阳性细菌。根据本发明可以应用这样的细菌和多糖，以提供用于疫苗接种的组合物，用于治疗和预防感染性细菌性疾病。

12. 发明申请

WO2005090396A1 NOVEL MANNOSE-SPECIFIC ADHESINS AND THEIR USE 审中-公开
标题翻译：新型特殊粘合剂及其使用
公开(公告)号：WO2005090396A1
公开(公告)日：2005-09-29
申请号：PCT/NL2005/000216
申请日：2005-03-22
申请人： WAGENINGEN CENTRE FOR FOOD SCIENCES , PRETZER, Gabriele , SNEL, Johannes , BRON, Peter, Allard , DE VOS, Willem, Meindert , KLEEREBEZEM, Michiel
发明人： PRETZER, Gabriele , SNEL, Johannes , BRON, Peter, Allard , DE VOS, Willem, Meindert , KLEEREBEZEM, Michiel
IPC分类号： C07K14/335
CPC分类号： C07K14/335 , A61K38/00
摘要： The present invention relates to novel mannose-specific adhesins, and variants thereof, as well as nucleic acid sequences encoding these. The invention also relates to host cells expressing nucleic acids encoding the mannose-specific adhesins or variants thereof, as well as to pharmaceutical or nutraceutical compositions comprising the mannose-specific adhesins and/or host cells expressing the adhesins, and their use in treating or preventing bacterial infection. Further, screening methods suitable for identifying bacteria strains with probiotic properties are provided.
摘要翻译：本发明涉及新型甘露糖特异性粘附素及其变体，以及编码这些粘附素的核酸序列。本发明还涉及表达编码甘露糖特异性粘附素或其变体的核酸的宿主细胞，以及包含表达粘附素的甘露糖特异性粘附素和/或宿主细胞的药物或营养组合物及其在治疗或预防中的用途细菌感染。此外，提供了适用于鉴定具有益生菌特性的细菌菌株的筛选方法。

13. 发明申请

WO2003076463A3 LISTERIA MONOCYTOGENES CTSR MUTANTS PRESENTING LOW VIRULENCE AND STRESS-RESISTANCE 审中-公开
公开(公告)号：WO2003076463A3
公开(公告)日：2003-09-18
申请号：PCT/NL2003/000178
申请日：2003-03-11
申请人： WAGENINGEN CENTRE FOR FOOD SCIENCES , KARATZAS, Kimon, Andreas , BENNIK, Maria, Henrica, Jacoba , ABEE, Tjakko , KLEEREBEZEM, Michiel , DE VOS, Willem, Meindert
发明人： KARATZAS, Kimon, Andreas , BENNIK, Maria, Henrica, Jacoba , ABEE, Tjakko , KLEEREBEZEM, Michiel , DE VOS, Willem, Meindert
IPC分类号： C12N15/31
摘要： The present invention relates to Gram positive bacteria with increased stress tolerance, reduced virulence, and/or reduced mobility, as a result of an altered CtsR protein. The alteration of the CtsR protein in particular concerns an alteration of the conserved glycine-rich region in the CtsR protein. The invention further relates to nucleic acid encoding the altered CtsR proteins. The altered Gram positive bacteria may be used in fermentation processes and may be used as delivery vehicles for site specific delivery of therapeutic proteins such as vaccines.

14. 发明申请

WO2002097063A1 PRODUCTION OF BIOAVAILABLE FOLIC ACID 审中-公开
标题翻译：生物可利用的氟酸
公开(公告)号：WO2002097063A1
公开(公告)日：2002-12-05
申请号：PCT/NL2002/000340
申请日：2002-05-28
申请人： STICHTING TOP-INSTITUUT VOEDSELWETENSCHAPPEN , SYBESMA, Wilbert, Feike, Henricus , HUGENHOLTZ, Jeroen , MIERAU, Igor , STARRENBURG, Maria, Johanna, Catharina , KLEEREBEZEM, Michiel , DE VOS, Willem, Meindert
发明人： SYBESMA, Wilbert, Feike, Henricus , HUGENHOLTZ, Jeroen , MIERAU, Igor , STARRENBURG, Maria, Johanna, Catharina , KLEEREBEZEM, Michiel , DE VOS, Willem, Meindert
IPC分类号： C12N1/00
CPC分类号： A61K38/45 , A23C2220/202 , A23L29/065 , A23L33/15 , A23V2002/00 , A61K38/50 , C12N9/1235 , C12N9/48 , C12N9/78 , C12N15/52 , C12P17/182 , A23V2300/21
摘要： The invention provides a process of producing bio-available folate, i.e. folic acid having an increased proportion of monoglutamyl folate and a decreased proportion of polyglutamyl folate, by culturing food-grade micro-organisms containing an active heterologous or homologous polyglutamyl hydrolase activity or containing increased activities of folate biosynthesis enzymes. The genes encoding the polyglutamyl hydrolase and the folate biosynthesis enzymes may be of various origin, e.g. from rodents or other mammals including man. Also provided is a foodstuff, especially a dairy product containing such monoglutamyl folate-producing micro-organisms.
摘要翻译：本发明提供一种通过培养含有活性异源或同源的聚谷氨酰水解酶活性的食品级微生物或含有增加的叶酸的方法，即生产叶酸的叶酸，其具有比例高的叶酸单谷氨酸和降低的聚谷氨酸叶酸的比例叶酸生物合成酶的活性。编码聚谷氨酸水解酶和叶酸生物合成酶的基因可以是各种来源的，例如。从啮齿动物或其他哺乳动物，包括人。还提供了一种食品，特别是含有这种产生叶酸单甲酯的微生物的乳制品。

15. 发明申请

WO2011059332A3 IMPROVED IMMUNOMODULATION BY PROBIOTICS 审中-公开
标题翻译：改进免疫细胞免疫调节
公开(公告)号：WO2011059332A3
公开(公告)日：2011-07-07
申请号：PCT/NL2010050760
申请日：2010-11-16
申请人： STICHTING TOP INST FOOD AND NUTRITION , VAN HEMERT SASKIA , MEIJERINK MARJOLEIN , BRON PETER ALLARD , MARCO MARIA LOUISE , KLEEREBEZEM MICHIEL , DE VOS PAULUS , WELS MICHIEL WOUTER WILHELMUS , WELLS JEREMY MARK
发明人： VAN HEMERT SASKIA , MEIJERINK MARJOLEIN , BRON PETER ALLARD , MARCO MARIA LOUISE , KLEEREBEZEM MICHIEL , DE VOS PAULUS , WELS MICHIEL WOUTER WILHELMUS , WELLS JEREMY MARK
IPC分类号： C12N15/74
CPC分类号： C12N1/36 , A23V2002/00 , A61K35/74 , C07K14/335 , C12N1/20 , C12N9/12 , A23V2200/324
摘要： The invention relates to a method for preparing a bacterium capable of immunomodulation, having either anti-inflammatory or pro -inflammatory capacities, by incorporating or knocking-out one or more genes. The invention also relates to modulating expression of these one or more genes by adapting fermentation conditions. The invention further provides for a method for identifying a bacterium capable of immunomodulation, a recombinant bacterium obtainable by any of such methods, compositions comprising such recombinant bacterium, and their use as a medicament. Also, the use of protein and nucleic acid sequences as a medicament is described.
摘要翻译：本发明涉及通过掺入或敲除一种或多种基因来制备能够进行免疫调节的具有抗炎或促炎能力的细菌的方法。本发明还涉及通过调节发酵条件来调节这些一种或多种基因的表达。本发明进一步提供了用于鉴定能够免疫调节的细菌的方法，可通过任何这些方法获得的重组细菌，包含这种重组细菌的组合物及其作为药物的用途。另外，描述了使用蛋白质和核酸序列作为药物。

16. 发明申请

WO2011059332A2 IMPROVED IMMUNOMODULATION BY PROBIOTICS 审中-公开
标题翻译：通过益生菌改进免疫调节
公开(公告)号：WO2011059332A2
公开(公告)日：2011-05-19
申请号：PCT/NL2010/050760
申请日：2010-11-16
申请人： STICHTING TOP INSTITUTE FOOD AND NUTRITION , VAN HEMERT, Saskia , MEIJERINK, Marjolein , BRON, Peter Allard , MARCO, Maria Louise , KLEEREBEZEM, Michiel , DE VOS, Paulus , WELS, Michiel Wouter Wilhelmus , WELLS, Jeremy Mark
发明人： VAN HEMERT, Saskia , MEIJERINK, Marjolein , BRON, Peter Allard , MARCO, Maria Louise , KLEEREBEZEM, Michiel , DE VOS, Paulus , WELS, Michiel Wouter Wilhelmus , WELLS, Jeremy Mark
IPC分类号： C12N15/74
CPC分类号： C12N1/36 , A23V2002/00 , A61K35/74 , C07K14/335 , C12N1/20 , C12N9/12 , A23V2200/324
摘要： The invention relates to a method for preparing a bacterium capable of immunomodulation, having either anti-inflammatory or pro -inflammatory capacities, by incorporating or knocking-out one or more genes. The invention also relates to modulating expression of these one or more genes by adapting fermentation conditions. The invention further provides for a method for identifying a bacterium capable of immunomodulation, a recombinant bacterium obtainable by any of such methods, compositions comprising such recombinant bacterium, and their use as a medicament. Also, the use of protein and nucleic acid sequences as a medicament is described.
摘要翻译：本发明涉及通过掺入或敲除一个或多个基因来制备具有抗炎或促炎症能力的免疫调节细菌的方法。本发明还涉及通过调整发酵条件来调节这些一个或多个基因的表达。本发明进一步提供了用于鉴定能够免疫调节的细菌的方法，通过任何这样的方法可获得的重组细菌，包含所述重组细菌的组合物，以及它们作为药物的用途。另外，描述了蛋白质和核酸序列作为药物的用途。

17. 发明申请

WO2009093900A1 MID-LOG PHASE LACTIC ACID BACTERIA FOR INDUCING IMMUNE TOLERANCE IN A SUBJECT 审中-公开
标题翻译：用于诱导受试者免疫耐受的中间相酸乳杆菌
公开(公告)号：WO2009093900A1
公开(公告)日：2009-07-30
申请号：PCT/NL2009/050028
申请日：2009-01-23
申请人： STICHTING TOP INSTITUTE FOOD AND NUTRITION , VAN BAARLEN, Peter , VAN DER MEER-KRAAIJ, Cindy Mathilda Jacoba , VAN HERMERT, Saskia , TROOST, Frederik Jan , SIKKEMA, Jan , BRUMMER, Robert-Jan , KLEEREBEZEM, Michiel
发明人： VAN BAARLEN, Peter , VAN DER MEER-KRAAIJ, Cindy Mathilda Jacoba , VAN HERMERT, Saskia , TROOST, Frederik Jan , SIKKEMA, Jan , BRUMMER, Robert-Jan , KLEEREBEZEM, Michiel
IPC分类号： A61K35/74 , A61P37/02
CPC分类号： A61K35/74 , A61K35/744
摘要： The invention relates to a composition comprising lactic acid bacteria, wherein a substantial amount thereof is in the mid-log phase, to its use for inducing immune tolerance and to a device for administering it.
摘要翻译：本发明涉及一种包含乳酸菌的组合物，其中大部分为对数中期，其用于诱导免疫耐受性的用途以及用于施用它的装置。

18. 发明申请

WO2008075949A1 MODULATION OF HUMAN MICROSOMAL TRIGLYCERIDE TRANSFER PROTEIN (MTP OR MTTP) GENE EXPRESSION BY FOOD-GRADE/INGESTED DIETARY MICROORGANISMS 审中-公开
标题翻译：人类微量TRIGLYCERIDE转移蛋白（MTP或MTTP）基因表达调控食品级食物摄入微生物
公开(公告)号：WO2008075949A1
公开(公告)日：2008-06-26
申请号：PCT/NL2007/050667
申请日：2007-12-18
申请人： STICHTING TOP INSTITUTE FOOD AND NUTRITION , TROOST, Frederik, Jan , KLEEREBEZEM, Michiel , BRUMMER, Robert-Jan , DE VOS, Willem, Meindert
发明人： TROOST, Frederik, Jan , KLEEREBEZEM, Michiel , BRUMMER, Robert-Jan , DE VOS, Willem, Meindert
IPC分类号： A61P1/00 , A61P3/04 , A61P9/00 , A61P9/10 , A61P3/08 , A61P3/10 , A61P1/14 , A61P37/00 , A61K35/74
CPC分类号： G01N33/92 , A61K35/747 , G01N33/5088 , G01N2333/335 , G01N2800/04
摘要： The present invention relates to the field of using microorganisms, especially food grade bacteria, to modulate intestinal MTP expression levels in order to treat and/or prevent weight gain, obesity, atherosclerosis, hyperglyceridaemia, hyper- cholesterolaemia, diabetes, dyslipidaemia and/or disorders associated with impaired intestinal immune response to antigens.
摘要翻译：本发明涉及使用微生物，特别是食品级细菌调节肠MTP表达水平以治疗和/或预防体重增加，肥胖，动脉粥样硬化，高甘油三酯血症，高胆固醇血症，糖尿病，血脂异常和/或病症的领域与抗原的肠道免疫应答受损。

19. 发明申请

WO2003012142A1 DETECTION OF NUCLEIC ACIDS BY REAL-TIME PCR USING CHIMERIC RNA-DNA PRIMERS 审中-公开
标题翻译：通过使用CHIMERIC RNA-DNA PRIMERS实时PCR检测核酸
公开(公告)号：WO2003012142A1
公开(公告)日：2003-02-13
申请号：PCT/NL2002/000519
申请日：2002-07-31
申请人： NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK , WAGENINGEN UNIVERSITY , SYBESMA, Wilbert, Feike, Henricus , KLEEREBEZEM, Michiel
发明人： SYBESMA, Wilbert, Feike, Henricus , KLEEREBEZEM, Michiel
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q2561/113 , C12Q2525/161
摘要： The present invention relates to methods for detection and quantification of target sequences in nucleic acid sample using real-time PCR. The method advantageously employs a chimeric RNA:DNA primer that introduces a unique (universal) detection sequence during a first gene-specific primer-extension reaction. The introduction of this unique detection sequence allows to use a single (universal) fluorescently labelled detection probe for detection and quantification of multiple different target sequence specific amplification products in real-time polymerase chain reactions. The method may be applied for detection and quantification of both DNA and RNA target sequences and is preferably used for quantification of specific mRNA's. By introducing a primer ligation step the method is adapted for genome wide transcriptome analysis. The method can be adapted for differential gene expression analysis in a single PCR reaction, allowing simultaneous and comparative analysis of specific gene-expression levels in samples obtained from cells grown under different conditions.
摘要翻译：本发明涉及使用实时PCR检测和定量核酸样品中靶序列的方法。该方法有利地采用在第一基因特异性引物延伸反应期间引入独特（通用）检测序列的嵌合RNA：DNA引物。引入这种独特的检测序列允许使用单一（通用）荧光标记的检测探针，用于在实时聚合酶链反应中检测和定量多种不同的靶序列特异性扩增产物。该方法可用于DNA和RNA靶序列的检测和定量，并且优选用于定量特异性mRNA。通过引入引物连接步骤，该方法适用于全基因组转录组学分析。该方法可以在单个PCR反应中适应差异基因表达分析，从而在从不同条件下生长的细胞获得的样品中同时和比较分析特异性基因表达水平。

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