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11. 发明专利

DK0934406T3 未知
公开(公告)号：DK0934406T3
公开(公告)日：2009-01-26
申请号：DK97936613
申请日：1997-08-25
申请人： JENSEN PETER RUHDAL
发明人： JENSEN PETER RUHDAL , HAMMER KARIN
IPC分类号： C12N15/09 , C12N15/11 , C12N15/10 , C12N15/63
摘要： An artificial promoter library for a selected organism or group of organisms is constructed as a mixture of double stranded DNA fragments, the sense strands of which comprise at least two consensus sequences of efficient promoters from said organism or group of organism, or parts thereof comprising at least half of each, and surrounding or intermediate nucleotide sequences (spacers) of variable length in which at least 7 nucleotides are selected randomly among the nucleobases A, T, C and G. The sense strands of the double stranded DNA fragments may also include a regulatory DNA sequence imparting a specific regulatory feature, such as activation by a change in the growth conditions, to the promoters of the library. Further, they may have a sequence comprising one or more recognition sites for restriction endonucleases added to one of or both their ends. The selected organism or group or organisms may be selected from prokaryotes and from eukaryotes; and in prokaryotes the consensus sequences to be retained most often will comprise the -35 signal (-35 to -30): TTGACA and the -10 signal (-12 to -7): TATAAT or parts of both comprising at least 3 conserved nucleotides of each, while in eukaryotes said consensus sequences should comprise a TATA box and at least one upstream activation sequence (UAS). Such artificial promoter libraries can be used i.e. for optimizing the expression of specific genes in various selected organism.

12. 发明专利

DE69739188D1 未知
公开(公告)号：DE69739188D1
公开(公告)日：2009-02-12
申请号：DE69739188
申请日：1997-09-08
申请人： JENSEN PETER RUHDAL
发明人： JENSEN PETER RUHDAL , SNOEP JACKY LEENDERT , WESTERHOFF HANS VICTOR
IPC分类号： C12N15/09 , C12P1/00 , C12N1/16 , C12N1/20 , C12N1/21 , C12N9/14 , C12N15/67 , C12Q1/68
摘要： The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in the cell and incubating the cell with a suitable substrate to produce the biomass or product. This is conveniently done by expressing in the cell the soluble part (F 1 ) of the membrane bound (F 0 F 1 type) H + ATPase or a portion of F 1 exhibiting ATPase activity. The organism from which the F 1 ATPase or portions thereof is derived, or in which the F 1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F 1 or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F 1 subunit beta or a portion thereof and various combinations of the gene or portion with the genes encoding the other F 1 subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing level of ATPase expression at which the conversion rate is optimized.

13. 发明专利

AT419371T 未知
公开(公告)号：AT419371T
公开(公告)日：2009-01-15
申请号：AT97938813
申请日：1997-09-08
申请人： JENSEN PETER RUHDAL
发明人： JENSEN PETER , SNOEP JACKY , WESTERHOFF HANS
IPC分类号： C12N15/09 , C12P1/00 , C12N1/16 , C12N1/20 , C12N1/21 , C12N9/14 , C12N15/67 , C12Q1/68
摘要： The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in the cell and incubating the cell with a suitable substrate to produce the biomass or product. This is conveniently done by expressing in the cell the soluble part (F 1 ) of the membrane bound (F 0 F 1 type) H + ATPase or a portion of F 1 exhibiting ATPase activity. The organism from which the F 1 ATPase or portions thereof is derived, or in which the F 1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F 1 or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F 1 subunit beta or a portion thereof and various combinations of the gene or portion with the genes encoding the other F 1 subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing level of ATPase expression at which the conversion rate is optimized.

14. 发明申请

WO1998010089A1 A METHOD OF IMPROVING THE PRODUCTION OF BIOMASS OR A DESIRED PRODUCT FROM A CELL 审中-公开
标题翻译：改善生物质生产或从细胞产生所需产品的方法
公开(公告)号：WO1998010089A1
公开(公告)日：1998-03-12
申请号：PCT/DK1997000373
申请日：1997-09-08
申请人： JENSEN, Peter, Ruhdal , SNOEP, Jacky, Leendert , WESTERHOFF, Hans, Victor
IPC分类号： C12P01/00
CPC分类号： C12N1/16 , C12N1/20 , C12N9/14 , C12P1/00
摘要： The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in said cell and incubating the cell with a suitable substrate to produce said biomass or product. This is conveniently done by expressing in said cell the soluble part (F1) of the membrane bound (F0F1 type) H -ATPase or a portion of F1 exhibiting ATPase activity. The organism from which the F1 ATPase or portions thereof is derived, or in which the F1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F1 or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F1 subunit beta or a portion thereof and various combinations of said gene or portion with the genes encoding the other F1 subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing a level of ATPase expression at which the conversion rate is optimized.
摘要翻译：通过诱导ATP向ADP的转化而不会对其它细胞代谢物或功能产生主要影响，可以改善生物量或细胞所需产物的产生，这通过在所述细胞中表达非偶联的ATP酶活性并将细胞与合适的底物以产生所述生物质或产品。通过在所述细胞中表达所结合的膜（F0F1型）H + - ATP酶的可溶性部分（F1）或显示出ATP酶活性的F1的一部分来方便地进行。 F1 ATP酶或其部分衍生自其中或其中表达F1 ATP酶或其部分的生物可以选自原核生物和真核生物。特别地，编码F1或其一部分的DNA可以衍生自细菌和真核微生物，例如高等生物的酵母，其他真菌和细胞系，并且可以选自编码F1亚单位β或其部分的基因和所述基因或部分与编码其它F1亚基或其部分的基因的各种组合。该方法可以使用i.a 通过在细胞中表达不同水平的解偶联的ATP酶活性来优化生物量或所需产物的形成，将细胞孵育在合适的底物上，测量底物转化成生物质或每种ATPase水平的所需产物的转化率表达，并选择转化率优化的ATPase表达水平。

15. 发明申请

WO2007082541A2 METHOD FOR THE DETECTION OF VIRUS INFECTED CELLS 审中-公开
标题翻译：用于检测病毒感染细胞的方法
公开(公告)号：WO2007082541A2
公开(公告)日：2007-07-26
申请号：PCT/DK2007/050001
申请日：2007-01-16
申请人： DANMARKS TEKNISKE UNIVERSITET , JENSEN, Peter Ruhdal , MICHELSEN, Ole
发明人： JENSEN, Peter Ruhdal , MICHELSEN, Ole
IPC分类号： C12Q1/04 , G01N15/14 , G01N35/00
CPC分类号： C12Q1/04 , G01N33/56983 , G01N2333/335
摘要： The present invention relates to a method for determining one or more stressed cell(s) in a medium. The method comprises the steps of (i) providing a sample from the medium, and (ii) determining a change in the sample relative to a normal sample of non-stressed cell(s).
摘要翻译：本发明涉及用于确定介质中的一个或多个应激细胞的方法。该方法包括以下步骤：（i）从介质提供样品，和（ii）确定样品相对于非应激细胞的正常样品的变化。

16. 发明申请

WO0202747A3 METHOD OF IMPROVING BIOMASS YIELD OF LACTIC ACID BACTERIAL CULTURES 审中-公开
标题翻译：提高乳酸菌细菌培养的生物量产量的方法
公开(公告)号：WO0202747A3
公开(公告)日：2002-05-10
申请号：PCT/DK0100468
申请日：2001-07-05
申请人： UNIV DANMARKS TEKNISKE , JENSEN PETER RUHDAL , BLANK LARS , KOEBMANN BRIAN JENSEN
发明人： JENSEN PETER RUHDAL , BLANK LARS , KOEBMANN BRIAN JENSEN
IPC分类号： C12N1/20 , C12N1/04
CPC分类号： C12N1/20
摘要： A method of enhancing biomass yield of a lactic acid bacterial species cell culture, comprising cultivating the cells in a process comprising the steps of providing conditions that results in a reduced glycolytic flux and providing conditions that enable the cells to have, under aerobic conditions, a respiratory metabolism. The increased yield of biomass may be the result of an increased yield of ATP which can be obtained by activating the native ATP synthase activity of the H -ATPase complex by lowering the ATP/ADP ratio, e.g. by carbon source limitation, and/or by increasing the proton gradient (membrane potential) of the cells, e.g. by enhancing or establishing an electron transport chain which can be achieved by enhancing expression of dehydrogenases or electron transport chain components, by adding to the medium a quinone or porphyrin compound or by enhancing the expression of the H -ATPase activity.
摘要翻译：提高乳酸细菌物种细胞培养物的生物质产量的方法，包括在包括以下步骤的方法中培养细胞：提供导致糖酵解通量降低的条件，并提供使细胞在有氧条件下具有呼吸代谢。增加的生物量产量可能是ATP产量增加的结果，其可通过降低ATP / ADP比率（例如通过降低ATP / ADP比率）活化H + -ATP酶复合物的天然ATP合酶活性而获得。通过碳源限制，和/或通过增加细胞的质子梯度（膜电位）通过增强或建立可通过增强脱氢酶或电子传递链组分的表达，通过向培养基中加入醌或卟啉化合物或通过增强H + -ATP酶活性的表达来实现的电子传递链。

17. 发明申请

WO1998007846A1 ARTIFICIAL PROMOTER LIBRARIES FOR SELECTED ORGANISMS AND PROMOTERS DERIVED FROM SUCH LIBRARIES 审中-公开
标题翻译：从这些图书馆衍生的选定的有机体和促销者的人造促进图书馆
公开(公告)号：WO1998007846A1
公开(公告)日：1998-02-26
申请号：PCT/DK1997000342
申请日：1997-08-25
申请人： JENSEN, Peter, Ruhdal , HAMMER, Karin
IPC分类号： C12N15/11
CPC分类号： C12N15/63 , C12N15/1051
摘要： An artificial promoter library for a selected organism or group of organisms is constructed as a mixture of double stranded DNA fragments, the sense strands of which comprise at least two consensus sequences of efficient promoters from said organism or group of organisms, or parts thereof comprising at least half of each, and surrounding or intermediate nucleotide sequences (spacers) of variable length in which at least 7 nucleotides are selected randomly among the nucleobases A, T, C and G. The sense strands of the double stranded DNA fragments may also include a regulatory DNA sequence imparting a specific regulatory feature, such as activation by a change in the growth conditions, to the promoters of the library. Further, they may have a sequence comprising one or more recognition sites for restriction endonucleases added to one of or both their ends. The selected organism or group or organisms may be selected from prokaryotes and from eukaryotes; and in prokaryotes the consensus sequences to be retained most often will comprise the -35 signal (-35 to -30): TTGACA and the -10 signal (-12 to -7): TATAAT or parts of both comprising at least 3 conserved nucleotides of each, while in eukaryotes said consensus sequences should comprise a TATA box and at least one upstream activation sequence (UAS). Such artificial promoter libraries can be used i.a. for optimizing the expression of specific genes in various selected organisms.
摘要翻译：构建用于所选生物体或生物体组的人造启动子文库，其为双链DNA片段的混合物，其有义链包含来自所述生物体或生物体组或其部分的至少两个有效启动子的共有序列，其包含每个的至少一半，以及在核碱基A，T，C和G中随机选择至少7个核苷酸的可变长度的周围或中间核苷酸序列（间隔区）。双链DNA片段的有义链还可以包括调节性DNA序列赋予特异性调节特征，例如通过生长条件改变而激活的文库启动子。此外，它们可以具有包含一个或多个加入其末端中的一个或两个的限制性内切核酸酶的识别位点的序列。所选择的生物体或组或生物体可以选自原核生物和真核生物; 并且在原核生物中，最常保留的共有序列将包含-35信号（-35至-30）：TTGACA和-10信号（-12至-7）：TATAAT或两者的部分包含至少3个保守核苷酸而在真核生物中，共有序列应包含TATA盒和至少一个上游激活序列（UAS）。这样的人造启动子文库可以在其中使用用于优化各种选择的生物体中特定基因的表达。

18. 发明申请

WO2007082541A3 METHOD FOR THE DETECTION OF VIRUS INFECTED CELLS 审中-公开
标题翻译：用于检测病毒感染细胞的方法
公开(公告)号：WO2007082541A3
公开(公告)日：2008-02-21
申请号：PCT/DK2007050001
申请日：2007-01-16
申请人： UNIV DANMARKS TEKNISKE , JENSEN PETER RUHDAL , MICHELSEN OLE
发明人： JENSEN PETER RUHDAL , MICHELSEN OLE
IPC分类号： G01N33/50 , C12Q1/04
CPC分类号： C12Q1/04 , G01N33/56983 , G01N2333/335
摘要： The present invention relates to a method for determining one or more stressed cell(s) in a medium. The method comprises the steps of (i) providing a sample from the medium, and (ii) determining a change in the sample relative to a normal sample of non-stressed cell(s).
摘要翻译：本发明涉及用于确定介质中的一个或多个应激细胞的方法。该方法包括以下步骤：（i）从介质提供样品，和（ii）确定样品相对于非应激细胞的正常样品的变化。

19. 发明申请

WO2006097107A1 LACTOSE-POSITIVE RECOMBINANT LEUCONOSTOC STRA 审中-公开
标题翻译：乳酸杆菌阳性重组体LEUCONOSTOC STRA
公开(公告)号：WO2006097107A1
公开(公告)日：2006-09-21
申请号：PCT/DK2006/000150
申请日：2006-03-15
申请人： TECHNICAL UNIVERSITY OF DENMARK , JENSEN, Peter, Ruhdal , HELMARK, Søren
发明人： JENSEN, Peter, Ruhdal , HELMARK, Søren
IPC分类号： C12N1/21 , A23L3/3571
CPC分类号： A23C17/02 , A23C13/16 , A23C19/0323 , A23Y2260/15 , C07K14/315 , C12N9/2445 , C12N9/2471 , C12Y302/01021 , C12Y302/01023
摘要： The present invention provides a Leuconostoc bacterial strain for use in the diary industry to prevent the growth of pathogenic micro-organisms and thereby ensure the quality and safety of milk products produced by fermentation, including cheese, yoghurt, sour cream, buttermilk and kefir products. The Leuconostoc bacterial strain of the invention is a recombinant Leuconostoc carnosum strain, characterised by a lactose-positive phenotype with the ability to utilise lactose as sole carbon source. The invention provides a starter culture comprising the lactose-positive Leuconostoc carnosum of the invention for use in the manufacture of milk products, and milk products prepared with said Leuconostoc strain or said starter culture.
摘要翻译：本发明提供了一种用于日记工业中用于防止致病微生物生长并由此确保通过发酵产生的乳制品（包括奶酪，酸奶，酸奶油，酪乳和牛奶制品）的质量和安全性的明串珠菌菌株。本发明的明串珠菌属细菌菌株是重组明串珠菌属Carnosum菌株，其特征在于具有利用乳糖作为唯一碳源的能力的乳糖阳性表型。本发明提供了一种起始培养物，其包含用于制造乳制品的本发明的乳糖阳性的明目菌肠藻和由所述明串珠菌属菌株或所述起始培养物制备的乳制品。

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