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1. 发明申请

WO2006097107A1 LACTOSE-POSITIVE RECOMBINANT LEUCONOSTOC STRA 审中-公开
标题翻译：乳酸杆菌阳性重组体LEUCONOSTOC STRA
公开(公告)号：WO2006097107A1
公开(公告)日：2006-09-21
申请号：PCT/DK2006/000150
申请日：2006-03-15
申请人： TECHNICAL UNIVERSITY OF DENMARK , JENSEN, Peter, Ruhdal , HELMARK, Søren
发明人： JENSEN, Peter, Ruhdal , HELMARK, Søren
IPC分类号： C12N1/21 , A23L3/3571
CPC分类号： A23C17/02 , A23C13/16 , A23C19/0323 , A23Y2260/15 , C07K14/315 , C12N9/2445 , C12N9/2471 , C12Y302/01021 , C12Y302/01023
摘要： The present invention provides a Leuconostoc bacterial strain for use in the diary industry to prevent the growth of pathogenic micro-organisms and thereby ensure the quality and safety of milk products produced by fermentation, including cheese, yoghurt, sour cream, buttermilk and kefir products. The Leuconostoc bacterial strain of the invention is a recombinant Leuconostoc carnosum strain, characterised by a lactose-positive phenotype with the ability to utilise lactose as sole carbon source. The invention provides a starter culture comprising the lactose-positive Leuconostoc carnosum of the invention for use in the manufacture of milk products, and milk products prepared with said Leuconostoc strain or said starter culture.
摘要翻译：本发明提供了一种用于日记工业中用于防止致病微生物生长并由此确保通过发酵产生的乳制品（包括奶酪，酸奶，酸奶油，酪乳和牛奶制品）的质量和安全性的明串珠菌菌株。本发明的明串珠菌属细菌菌株是重组明串珠菌属Carnosum菌株，其特征在于具有利用乳糖作为唯一碳源的能力的乳糖阳性表型。本发明提供了一种起始培养物，其包含用于制造乳制品的本发明的乳糖阳性的明目菌肠藻和由所述明串珠菌属菌株或所述起始培养物制备的乳制品。

2. 发明公开

EP0939830A1 A METHOD OF IMPROVING THE PRODUCTION OF BIOMASS OR A DESIRED PRODUCT FROM A CELL 失效
标题翻译：方法提高所需产物的制备从细胞的
公开(公告)号：EP0939830A1
公开(公告)日：1999-09-08
申请号：EP97938813.0
申请日：1997-09-08
申请人： Jensen, Peter Ruhdal
发明人： JENSEN, Peter, Ruhdal , SNOEP, Jacky, Leendert , WESTERHOFF, Hans, Victor
IPC分类号： C12N15 , C12N1 , C12N9 , C12P1 , C12Q1
CPC分类号： C12N1/16 , C12N1/20 , C12N9/14 , C12P1/00
摘要： The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in said cell and incubating the cell with a suitable substrate to produce said biomass or product. This is conveniently done by expressing in said cell the soluble part (F1) of the membrane bound (F0F1 type) H+-ATPase or a portion of F¿1? exhibiting ATPase activity. The organism from which the F1 ATPase or portions thereof is derived, or in which the F1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F1 or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F1 subunit β or a portion thereof and various combinations of said gene or portion with the genes encoding the other F1 subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing a level of ATPase expression at which the conversion rate is optimized.

3. 发明授权

EP0939830B1 A METHOD OF IMPROVING THE PRODUCTION OF A DESIRED PRODUCT FROM A CELL 失效
标题翻译：方法提高所需产物的制备从细胞的
公开(公告)号：EP0939830B1
公开(公告)日：2008-12-31
申请号：EP97938813.9
申请日：1997-09-08
申请人： Jensen, Peter Ruhdal
发明人： JENSEN, Peter, Ruhdal , SNOEP, Jacky, Leendert , WESTERHOFF, Hans, Victor
IPC分类号： C12P1/00 , C12N15/67
CPC分类号： C12N1/16 , C12N1/20 , C12N9/14 , C12P1/00
摘要： The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in said cell and incubating the cell with a suitable substrate to produce said biomass or product. This is conveniently done by expressing in said cell the soluble part (F1) of the membrane bound (F0F1 type) H -ATPase or a portion of F1 exhibiting ATPase activity. The organism from which the F1 ATPase or portions thereof is derived, or in which the F1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F1 or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F1 subunit beta or a portion thereof and various combinations of said gene or portion with the genes encoding the other F1 subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing a level of ATPase expression at which the conversion rate is optimized.

4. 发明授权

EP1863902B1 LACTOSE-POSITIVE RECOMBINANT LEUCONOSTOC STRA 有权
标题翻译： LACTOSEPOSITIVER重组明串珠菌TRIBE
公开(公告)号：EP1863902B1
公开(公告)日：2011-05-18
申请号：EP06706121.8
申请日：2006-03-15
申请人： The Technical University of Denmark
发明人： JENSEN, Peter, Ruhdal , HELMARK, Søren
IPC分类号： C12N1/21 , A23L3/3571
CPC分类号： A23C17/02 , A23C13/16 , A23C19/0323 , A23Y2260/15 , C07K14/315 , C12N9/2445 , C12N9/2471 , C12Y302/01021 , C12Y302/01023
摘要： The present invention provides a Leuconostoc bacterial strain for use in the diary industry to prevent the growth of pathogenic micro-organisms and thereby ensure the quality and safety of milk products produced by fermentation, including cheese, yoghurt, sour cream, buttermilk and kefir products. The Leuconostoc bacterial strain of the invention is a recombinant Leuconostoc carnosum strain, characterised by a lactose-positive phenotype with the ability to utilise lactose as sole carbon source. The invention provides a starter culture comprising the lactose-positive Leuconostoc carnosum of the invention for use in the manufacture of milk products, and milk products prepared with said Leuconostoc strain or said starter culture.

5. 发明公开

EP1863902A1 LACTOSE-POSITIVE RECOMBINANT LEUCONOSTOC STRA 有权
标题翻译： LactosepOSITIVER REKOMBINANTER LEUCONOSTOC-STAMM
公开(公告)号：EP1863902A1
公开(公告)日：2007-12-12
申请号：EP06706121.8
申请日：2006-03-15
申请人： The Technical University of Denmark
发明人： JENSEN, Peter, Ruhdal , HELMARK, Søren
IPC分类号： C12N1/21 , A23L3/3571
CPC分类号： A23C17/02 , A23C13/16 , A23C19/0323 , A23Y2260/15 , C07K14/315 , C12N9/2445 , C12N9/2471 , C12Y302/01021 , C12Y302/01023
摘要： The present invention provides a Leuconostoc bacterial strain for use in the diary industry to prevent the growth of pathogenic micro-organisms and thereby ensure the quality and safety of milk products produced by fermentation, including cheese, yoghurt, sour cream, buttermilk and kefir products. The Leuconostoc bacterial strain of the invention is a recombinant Leuconostoc carnosum strain, characterised by a lactose-positive phenotype with the ability to utilise lactose as sole carbon source. The invention provides a starter culture comprising the lactose-positive Leuconostoc carnosum of the invention for use in the manufacture of milk products, and milk products prepared with said Leuconostoc strain or said starter culture.
摘要翻译：本发明提供了一种用于日记工业中用于防止病原微生物生长并由此确保通过发酵产生的乳制品（包括奶酪，酸奶，酸奶油，酪乳和牛奶制品）的质量和安全性的明串珠菌菌株。本发明的明串珠菌属细菌菌株是一种重组明串珠菌属Carnosum菌株，其特征在于具有利用乳糖作为唯一碳源的能力的乳糖阳性表型。本发明提供一种起始培养物，其包含用于制造乳制品的本发明的乳糖阳性明目片藻属，以及由所述明串珠菌属菌株或所述起始培养物制备的乳制品。

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