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1. 发明申请

WO1998010089A1 A METHOD OF IMPROVING THE PRODUCTION OF BIOMASS OR A DESIRED PRODUCT FROM A CELL 审中-公开
标题翻译：改善生物质生产或从细胞产生所需产品的方法
公开(公告)号：WO1998010089A1
公开(公告)日：1998-03-12
申请号：PCT/DK1997000373
申请日：1997-09-08
申请人： JENSEN, Peter, Ruhdal , SNOEP, Jacky, Leendert , WESTERHOFF, Hans, Victor
IPC分类号： C12P01/00
CPC分类号： C12N1/16 , C12N1/20 , C12N9/14 , C12P1/00
摘要： The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in said cell and incubating the cell with a suitable substrate to produce said biomass or product. This is conveniently done by expressing in said cell the soluble part (F1) of the membrane bound (F0F1 type) H -ATPase or a portion of F1 exhibiting ATPase activity. The organism from which the F1 ATPase or portions thereof is derived, or in which the F1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F1 or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F1 subunit beta or a portion thereof and various combinations of said gene or portion with the genes encoding the other F1 subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing a level of ATPase expression at which the conversion rate is optimized.
摘要翻译：通过诱导ATP向ADP的转化而不会对其它细胞代谢物或功能产生主要影响，可以改善生物量或细胞所需产物的产生，这通过在所述细胞中表达非偶联的ATP酶活性并将细胞与合适的底物以产生所述生物质或产品。通过在所述细胞中表达所结合的膜（F0F1型）H + - ATP酶的可溶性部分（F1）或显示出ATP酶活性的F1的一部分来方便地进行。 F1 ATP酶或其部分衍生自其中或其中表达F1 ATP酶或其部分的生物可以选自原核生物和真核生物。特别地，编码F1或其一部分的DNA可以衍生自细菌和真核微生物，例如高等生物的酵母，其他真菌和细胞系，并且可以选自编码F1亚单位β或其部分的基因和所述基因或部分与编码其它F1亚基或其部分的基因的各种组合。该方法可以使用i.a 通过在细胞中表达不同水平的解偶联的ATP酶活性来优化生物量或所需产物的形成，将细胞孵育在合适的底物上，测量底物转化成生物质或每种ATPase水平的所需产物的转化率表达，并选择转化率优化的ATPase表达水平。

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