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171. 发明授权

US08123925B2 Electrophoretic apparatus 有权
标题翻译：电泳仪
公开(公告)号：US08123925B2
公开(公告)日：2012-02-28
申请号：US11984719
申请日：2007-11-21
申请人： Tomohiro Shoji , Jin Matsumura , Hidenori Namba
发明人： Tomohiro Shoji , Jin Matsumura , Hidenori Namba
IPC分类号： G01N27/453
CPC分类号： G01N27/44782
摘要： An electrophoretic apparatus that allows bubbles to be readily removed out of an electrophoretic passage. The passage for electrophoretic medium, at a connecting section where capillaries filled with electrophoretic medium are connected with a pumping mechanism for filling the electrophoretic medium, is arranged such that the side of the pumping mechanism is disposed below the side of the capillaries, so that the electrophoretic medium flows from down to up at the connecting section when filling the electrophoretic medium into the capillaries. Preferably, the passage between the capillary array and the buffer solution is controlled by using a rotary-type valve having high withstand pressure to simplify the passage structure. The dead volume of the passage can be reduced and the valuable electrophoretic medium can be efficiently used. The amount of used electrophoretic medium required for the removal of the bubble can be also reduced.
摘要翻译：允许气泡容易地从电泳通道中除去的电泳装置。电泳介质的通道在填充有电泳介质的毛细管的连接部分处连接有用于填充电泳介质的泵送机构，使得泵送机构的一侧设置在毛细管侧面，使得当将电泳介质填充到毛细管中时，电泳介质在连接部分从下向上流动。优选地，通过使用具有高耐压的旋转式阀来控制毛细管阵列和缓冲溶液之间的通道，以简化通道结构。可以减少通道的死体积并且可以有效地使用有价值的电泳介质。除去气泡所需的电泳介质的用量也可以减少。

172. 发明申请

US20110253535A1 NON-GEL BASED TWO-DIMENSIONAL PROTEIN SEPARATION MULTI-CHANNEL DEVICES 有权
标题翻译：非凝胶二维蛋白分离多通道器件
公开(公告)号：US20110253535A1
公开(公告)日：2011-10-20
申请号：US12531281
申请日：2009-05-25
申请人： Myeong Hee Moon , Ki Hun Kim
发明人： Myeong Hee Moon , Ki Hun Kim
IPC分类号： C25B7/00 , G01N27/447
CPC分类号： G01N27/44773 , G01N27/44782 , G01N27/44795 , G01N30/0005 , G01N35/1097 , G01N2030/003
摘要： Provided is a multi-channel apparatus for non-gel based two-dimensional protein separation. One or more flat channels are arranged in parallel and have an isoelectric focusing section for primarily separating proteins from protein samples according to isoelectric point (pI) and a flow field-flow fractionation section for secondarily separating the primarily separated proteins according to molecular weight, thereby making it possible to simultaneously separate the proteins in multiple channels, to increase a protein separation speed, and to overcome limitation to sample injection due to an increase in channel volume. The apparatus can separate the proteins according to pI and molecular weight, be safe from denaturation of the protein in the process of protein separation, automatically remove an ampholyte used for pI-based separation, and separately detecting the separated proteins to identify the proteins using nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS).
摘要翻译：提供了一种用于非凝胶二维蛋白分离的多通道装置。一个或多个扁平通道平行布置并具有等电聚焦部分，用于根据等电点（pI）和流场分流分离部分主要从蛋白质样品分离蛋白质，以便根据分子量二次分离主要分离的蛋白质，从而使得可以同时分离多个通道中的蛋白质，以增加蛋白质分离速度，并且克服由于通道体积的增加而对样品注入的限制。该装置可以根据pI和分子量分离蛋白质，蛋白质分离过程中蛋白质的变性是安全的，自动去除用于pI的分离的两性电解质，并分别检测分离的蛋白质以使用nanoflow鉴定蛋白质液相色谱 - 电喷雾离子化 - 串联质谱（LC-ESI-MS-MS）。

173. 发明授权

US08003076B2 Methods for conducting metabolic analyses 失效
标题翻译：进行代谢分析的方法
公开(公告)号：US08003076B2
公开(公告)日：2011-08-23
申请号：US12146328
申请日：2008-06-25
申请人： Luke V. Schneider
发明人： Luke V. Schneider
IPC分类号： A61K51/00
CPC分类号： G01N33/6848 , B65G59/066 , G01N27/44717 , G01N27/44726 , G01N27/44773 , G01N27/44782 , G01N27/44795 , Y10S435/968 , Y10S435/973
摘要： The present invention provides methods and apparatus for purifying metabolites of interest and conducting metabolic analyses. The methods generally involve determining metabolic flux values for a plurality of target analytes by monitoring the relative isotope abundance of a stable isotope in a substrate labeled with the stable isotope and/or one or more target metabolites formed through metabolism of the labeled substrate. Certain methods utilize multiple electrophoretic methods to separate the target analytes from other components within the sample being analyzed. The methods can be used in a variety of applications including screens to identify metabolites that are correlated with certain diseases and diagnostic screens for identifying individuals having, or susceptible to, a disease.
摘要翻译：本发明提供了用于纯化目的代谢物和进行代谢分析的方法和装置。所述方法通常涉及通过监测用稳定同位素标记的底物和/或通过标记的底物代谢形成的一种或多种目标代谢物来监测稳定同位素的相对同位素丰度来确定多种目标分析物的代谢通量值。某些方法利用多种电泳方法将目标分析物与正在分析的样品中的其他组分分离。所述方法可用于各种应用，包括筛选鉴定与某些疾病相关的代谢物和用于识别具有或易感染疾病的个体的诊断筛选的筛选。

174. 发明授权

US07931789B2 Device for charging separation buffer liquid to microchip, and microchip processing device equipped with the charging device, electrophoresis method in capillary channel and its microchip processing device 有权
标题翻译：用于将分离缓冲液充入微芯片的装置，以及配备充电装置的微芯片处理装置，毛细通道中的电泳方法及其微芯片处理装置
公开(公告)号：US07931789B2
公开(公告)日：2011-04-26
申请号：US11493810
申请日：2006-07-27
申请人： Nobuhiro Hanafusa , Tomokazu Sudo , Katsuhiko Seki , Koichi Suzuki
发明人： Nobuhiro Hanafusa , Tomokazu Sudo , Katsuhiko Seki , Koichi Suzuki
IPC分类号： G01N27/447
CPC分类号： C07K1/26 , G01N27/44743 , G01N27/44756 , G01N27/44782 , G01N27/44791
摘要： In a separation buffer solution filling device, a microchip is arranged such reservoirs are opened on a surface on respective ends of channels including at least a main separation channel in which analysis is performed while a solution moves inside a plate-like member, and the reservoirs face upward. The filling device fills separation buffer solution into the channels by supplying air from an air supply port which is pushed while maintaining air-tightness onto a top of the reservoir filled with the separation buffer solution on either end of said channels. The air supply port is an opening on a front end of an air cylinder, and has a seal part on that opening. The filling device is pushed onto the reservoir while maintaining air-tightness by that seal part.
摘要翻译：在分离缓冲溶液填充装置中，布置微芯片，这些储存器在通道的相应端部的表面上开口，该通道包括至少一个主分离通道，其中在溶液移动到板状构件内部进行分析，并且储存器面朝上填充装置通过从保持气密性的空气供应口供给空气而将分离缓冲溶液填充到通道中，所述空气供应口在所述通道的任一端上填充有分离缓冲溶液的容器的顶部。空气供给口是气缸前端的开口，在该开口部具有密封部。将填充装置推压到储存器上，同时保持该密封部分的气密性。

175. 发明授权

US07856896B2 Analyzer 有权
标题翻译：分析仪
公开(公告)号：US07856896B2
公开(公告)日：2010-12-28
申请号：US11989649
申请日：2006-07-27
申请人： Daisuke Matsumoto
发明人： Daisuke Matsumoto
IPC分类号： G01N1/38 , G01N21/01 , G01N21/29
CPC分类号： G01N27/44782 , G01N35/08
摘要： The present invention relates to an analytical tool including a plurality of flow paths 21 each including a detection cell 26 for detecting a particular component contained in a sample and configured to move the sample by capillarity, and a common path 22 connecting the plurality of flow paths 21 to each other. Each of the flow paths 21 includes a water stopper 29 for preventing the sample from flowing into the common path 22. Preferably, the water stopper 29 suppresses the movement of the sample by differentiating a cross sectional area at a target portion in a direction perpendicular to the sample flow direction from a cross sectional area at a portion near the target portion.
摘要翻译：本发明涉及一种分析工具，其包括多个流路21，每个流路21包括用于检测样品中包含的特定组分的检测单元26，并且被配置为通过毛细管作用移动样品;以及公共路径22，连接多个流路 21对方每个流路21包括用于防止样品流入公共路径22的止水器29.优选地，止水器29通过在垂直于...的方向上区分目标部分处的横截面积来抑制样品的移动样品流动方向从目标部分附近的部分的横截面积。

176. 发明授权

US07854827B2 Comparative multidimensional gel electrophoresis 有权
标题翻译：比较多维凝胶电泳
公开(公告)号：US07854827B2
公开(公告)日：2010-12-21
申请号：US11642242
申请日：2006-12-20
申请人： Mario Curcio
发明人： Mario Curcio
IPC分类号： C25B7/00 , C07K1/26 , C07K1/28
CPC分类号： G01N27/44795 , B01L3/5027 , G01N27/44773 , G01N27/44782
摘要： A device providing an arrangement for the separation of a sample mixture for analytical reasons based on multidimensional gel electrophoresis and method thereof are disclosed. The separation involves a first separation in a first dimension of the device on the basis of isoelectric points and a second separation in a second dimension of the device on the basis of molecular size. At least two gel strips for the first dimension separation step and a corresponding gel for the second dimension separation step are provided. The two gel strips are arranged on a single carrier either on the same side or on opposite sides. By having at least two gel strips arranged in such a manner at least two analytical processes can be executed in parallel on the single carrier without the use of valves.
摘要翻译：公开了一种基于多维凝胶电泳提供用于分离试样混合物的装置的装置及其方法。分离包括基于等电点在装置的第一维度中的第一分离，并且基于分子尺寸在装置的第二维度中进行第二分离。提供用于第一维分离步骤的至少两个凝胶条和用于第二维分离步骤的相应凝胶。两个凝胶条布置在同一侧或相对侧上的单个载体上。通过使至少两个凝胶条以这种方式布置，可以在不使用阀的情况下在单个载体上并行地执行至少两个分析过程。

177. 发明申请

US20100116659A1 Multidimensional Separations Employing an Array of Electrophoresis Channels 审中-公开
标题翻译：使用电泳通道阵列的多维分离
公开(公告)号：US20100116659A1
公开(公告)日：2010-05-13
申请号：US12495513
申请日：2009-06-30
申请人： ChangSheng Liu , Kevin Le Van
发明人： ChangSheng Liu , Kevin Le Van
IPC分类号： B01D57/02 , G01N27/00
CPC分类号： G01N27/44782 , G01N27/44721 , G01N27/44743 , G01N27/44773
摘要： The present invention relates to a method for separating components of a sample. The method includes obtaining a first separation of the sample components along a first dimension wherein the sample components are at least partially resolved, wherein the first separation can be performed in the absence of an electric field applied to the first dimension. An electric field is used to obtain a second separation of the sample components along a second dimension comprising a plurality of substantially isolated volumes. An intensity-time data record is obtained from each of the isolated volumes, the intensity-time data records containing peaks, each peak being indicative of a migration time. The migration time of a first peak is normalized with respect to a migration time of at least a second peak to correct for migration time differences between the isolated volumes.
摘要翻译：本发明涉及一种分离样品组分的方法。该方法包括沿第一维度获得样品组分的第一分离，其中样品组分至少部分被分离，其中第一分离可以在没有施加到第一维度的电场的情况下进行。电场用于沿着包括多个基本上隔离的体积的第二维度获得样品组分的第二分离。从每个孤立的体积获得强度时间数据记录，强度 - 时间数据记录包含峰值，每个峰值表示迁移时间。相对于至少第二峰的迁移时间，第一峰的迁移时间被归一化以校正隔离体积之间的迁移时间差。

178. 发明授权

US07622083B2 Multi-capillary electrophoresis cartridge interface mechanism 有权
标题翻译：多毛细管电泳盒接口机理
公开(公告)号：US07622083B2
公开(公告)日：2009-11-24
申请号：US10823382
申请日：2004-04-12
申请人： Varouj Amirkhanian , Bob G. Heitel , Ming-Sun Liu , Paul Mooney
发明人： Varouj Amirkhanian , Bob G. Heitel , Ming-Sun Liu , Paul Mooney
IPC分类号： B01L11/00
CPC分类号： G01N27/44782 , G01N27/44721 , G01N2021/6463 , G01N2021/6484 , G01N2201/08
摘要： The present invention provides for an interface mechanism in a bio-separation instrument that makes interface connections to a multi-channel cartridge. The interface mechanism precisely positions the cartridge in relation to the support elements in the instrument (e.g., high-voltage, gas pressure, incident radiation and detector), and makes automated, reliable and secured alignments and connections between various components in the cartridge and the support elements in the supporting instrument. The interface mechanism comprises pneumatically or electromechanically driven actuators for engaging support elements in the instrument to components on the cartridge. After the cartridge has been securely received by the interface mechanism, the connection sequence is initiated. The interface provides separate high voltage and optical connections for each separation channel in the cartridge, thus providing channel-to-channel isolation from cross talk both electrically and optically.
摘要翻译：本发明提供了生物分离仪器中的接口机构，其使接口连接到多通道盒。接口机构将盒相对于仪器中的支撑元件（例如，高压，气体压力，入射辐射和检测器）精确定位，并且使得盒中的各种部件之间的自动化，可靠和可靠的对准和连接配套仪器中的支撑元件。接口机构包括气动或机电驱动的致动器，用于将仪器中的支撑元件接合到盒上的部件。在通过接口机构安全接收到盒式磁带后，启动连接顺序。该接口为盒中的每个分离通道提供单独的高电压和光学连接，从而提供电气和光学上的串扰通道间通道隔离。

179. 发明授权

US07531076B2 Capillary cassette and method of manufacturing the same 失效
标题翻译：毛细管盒及其制造方法
公开(公告)号：US07531076B2
公开(公告)日：2009-05-12
申请号：US11326368
申请日：2006-01-06
申请人： Yoshihide Hayashizaki , Rintaro Yamamoto
发明人： Yoshihide Hayashizaki , Rintaro Yamamoto
IPC分类号： G01N27/453
CPC分类号： G01N27/44782 , Y10S83/95 , Y10T29/5148 , Y10T29/53391 , Y10T83/0596
摘要： Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. it fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.
摘要翻译：毛细管柱（102）穿过并被插入橡胶板（14）中，橡胶板通过橡胶的弹力保持和固定，并且二维地布置在样品注射侧。它通过将保持板（6a）从下方夹持而在安装在平面上的毛细管柱（102）上固定，并在检测侧从橡胶板（16）上方固定。为了将毛细管柱（102）压靠在保持板6a上并将其固定在橡胶板（16）上，将橡胶板（16）固定在两侧的保持板（6a）上的保持板（6b）提供毛细管柱（102）的布置。

180. 发明申请

US20090071830A1 Systems and Methods for Isolating Nucleic Acids 失效
标题翻译：用于分离核酸的系统和方法
公开(公告)号：US20090071830A1
公开(公告)日：2009-03-19
申请号：US12179455
申请日：2008-07-24
申请人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
发明人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
IPC分类号： B01D57/02 , B01D61/42 , C25B9/08
CPC分类号： C12N15/101 , G01N27/44782
摘要： A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber. The system may further include a pair of electrodes configured to generate an electric field sufficient to cause target nucleic acids in the at least one sample chamber to migrate via electrophoresis from the at least one sample chamber through the filter into the at least one collection chamber, wherein the filter may be configured to permit passage of target nucleic acids and to block passage of material of a size larger than the target nucleic acids.
摘要翻译：用于从样品收集目标核酸的系统可以包括至少一个样品室，其被配置为接收含有靶核酸和其它材料的样品，至少一个收集室，其相对于至少一个样品室可拆卸地安装并且被配置成收集目标与其他材料分离的核酸，相对于至少一个样品室可拆卸地安装的过滤器，并且被配置为当所述至少一个收集室相对于所述至少一个收集室安装时，被设置在所述至少一个样品室和所述至少一个收集室之间所述至少一个样品室。该系统可以进一步包括一对电极，其被配置为产生足以使所述至少一个样品室中的靶核酸经由电泳从所述至少一个样品室通过所述过滤器迁移到所述至少一个收集室中的电场，其中所述过滤器可以被配置为允许靶核酸的通过并阻止尺寸大于所述靶核酸的材料的通过。

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