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1. 发明授权

US08568580B2 Systems and methods for isolating nucleic acids 失效
标题翻译：用于分离核酸的系统和方法
公开(公告)号：US08568580B2
公开(公告)日：2013-10-29
申请号：US12179455
申请日：2008-07-24
申请人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
发明人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
IPC分类号： G01N27/453 , B01D61/42
CPC分类号： C12N15/101 , G01N27/44782
摘要： A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber. The system may further include a pair of electrodes configured to generate an electric field sufficient to cause target nucleic acids in the at least one sample chamber to migrate via electrophoresis from the at least one sample chamber through the filter into the at least one collection chamber, wherein the filter may be configured to permit passage of target nucleic acids and to block passage of material of a size larger than the target nucleic acids.
摘要翻译：用于从样品收集目标核酸的系统可以包括至少一个样品室，其被配置为接收含有靶核酸和其它材料的样品，至少一个收集室，其相对于至少一个样品室可拆卸地安装并且被配置成收集目标与其他材料分离的核酸，相对于至少一个样品室可拆卸地安装的过滤器，并且被配置为当所述至少一个收集室相对于所述至少一个收集室安装时，被设置在所述至少一个样品室和所述至少一个收集室之间所述至少一个样品室。该系统可以进一步包括一对电极，其被配置为产生足以使所述至少一个样品室中的靶核酸经由电泳从所述至少一个样品室通过所述过滤器迁移到所述至少一个收集室中的电场，其中所述过滤器可以被配置为允许靶核酸的通过并阻止尺寸大于所述靶核酸的材料的通过。

2. 发明申请

US20090071830A1 Systems and Methods for Isolating Nucleic Acids 失效
标题翻译：用于分离核酸的系统和方法
公开(公告)号：US20090071830A1
公开(公告)日：2009-03-19
申请号：US12179455
申请日：2008-07-24
申请人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
发明人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
IPC分类号： B01D57/02 , B01D61/42 , C25B9/08
CPC分类号： C12N15/101 , G01N27/44782
摘要： A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber. The system may further include a pair of electrodes configured to generate an electric field sufficient to cause target nucleic acids in the at least one sample chamber to migrate via electrophoresis from the at least one sample chamber through the filter into the at least one collection chamber, wherein the filter may be configured to permit passage of target nucleic acids and to block passage of material of a size larger than the target nucleic acids.
摘要翻译：用于从样品收集目标核酸的系统可以包括至少一个样品室，其被配置为接收含有靶核酸和其它材料的样品，至少一个收集室，其相对于至少一个样品室可拆卸地安装并且被配置成收集目标与其他材料分离的核酸，相对于至少一个样品室可拆卸地安装的过滤器，并且被配置为当所述至少一个收集室相对于所述至少一个收集室安装时，被设置在所述至少一个样品室和所述至少一个收集室之间所述至少一个样品室。该系统可以进一步包括一对电极，其被配置为产生足以使所述至少一个样品室中的靶核酸经由电泳从所述至少一个样品室通过所述过滤器迁移到所述至少一个收集室中的电场，其中所述过滤器可以被配置为允许靶核酸的通过并阻止尺寸大于所述靶核酸的材料的通过。

3. 发明授权

US08535878B2 Methods, compositions, and kits for detecting protein aggregates 有权
标题翻译：用于检测蛋白质聚集体的方法，组合物和试剂盒
公开(公告)号：US08535878B2
公开(公告)日：2013-09-17
申请号：US11743517
申请日：2007-05-02
申请人： David W. Ruff , Mark E. Shannon , Kenneth J. Livak , Karl J. Guegler , Kevin M. Hennessy
发明人： David W. Ruff , Mark E. Shannon , Kenneth J. Livak , Karl J. Guegler , Kevin M. Hennessy
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C07K14/47 , C07K14/4711 , C12Q1/6816 , C12Q2533/107
摘要： The present teachings provide methods, compositions, and kits for detecting the presence of protein aggregates. In some embodiments, the protein aggregate is treated with a labeled precursor, and the labeled precursor is incorporated into the protein aggregate to form a labeled protein aggregate. The labeled protein aggregate is then measured, thus detecting the presence of the protein aggregate. In some embodiments, the labeled protein aggregate is detected by interaction of labeled precursors, for example by a proximity ligation assay.
摘要翻译：本教导提供了用于检测蛋白质聚集体的存在的方法，组合物和试剂盒。在一些实施方案中，用标记的前体处理蛋白质聚集体，并将标记的前体掺入蛋白质聚集体中以形成标记的蛋白质聚集体。然后测量标记的蛋白质聚集体，从而检测蛋白质聚集体的存在。在一些实施方案中，标记的蛋白质聚集体通过标记的前体的相互作用来检测，例如通过邻近连接测定。

4. 发明申请

US20100036616A1 Normalization of Gene Expression Data 审中-公开
标题翻译：基因表达数据的归一化
公开(公告)号：US20100036616A1
公开(公告)日：2010-02-11
申请号：US12558428
申请日：2009-09-11
申请人： Mark E. Shannon , Mark F. Oldham , David W. Ruff
发明人： Mark E. Shannon , Mark F. Oldham , David W. Ruff
IPC分类号： G06F19/00
CPC分类号： G06F19/20 , C12Q1/686 , C40B30/04
摘要： A method for determining bias across two domains comprising gene expression data. The method can comprise (a) providing a first domain and a second domain; (b) obtaining information indicative of a bias within the first domain; (c) obtaining information indicative of a bias within the second domain; and (d) using the information indicative of the bias within the first domain and the information indicative of the bias within the second domain to produce an indication of bias across the two domains.
摘要翻译：一种用于确定包含基因表达数据的两个结构域之间的偏差的方法。该方法可以包括（a）提供第一域和第二域; （b）获得指示第一域内偏倚的信息; （c）获得指示第二域内偏倚的信息; 以及（d）使用指示第一域内的偏差的信息和指示第二域内的偏差的信息来产生跨越两个域的偏差的指示。

5. 发明授权

US09026371B2 Method for cross-instrument comparison of gene expression data 有权
标题翻译：交叉仪器比较基因表达数据的方法
公开(公告)号：US09026371B2
公开(公告)日：2015-05-05
申请号：US12558428
申请日：2009-09-11
申请人： Mark E. Shannon , Mark F. Oldham , David W. Ruff
发明人： Mark E. Shannon , Mark F. Oldham , David W. Ruff
IPC分类号： G01N33/48 , C40B30/04 , G06F19/20 , C12Q1/68
CPC分类号： G06F19/20 , C12Q1/686 , C40B30/04
摘要： A method for determining bias across two domains comprising gene expression data. The method can comprise (a) providing a first domain and a second domain; (b) obtaining information indicative of a bias within the first domain; (c) obtaining information indicative of a bias within the second domain; and (d) using the information indicative of the bias within the first domain and the information indicative of the bias within the second domain to produce an indication of bias across the two domains.
摘要翻译：一种用于确定包含基因表达数据的两个结构域之间的偏差的方法。该方法可以包括（a）提供第一域和第二域; （b）获得指示第一域内偏倚的信息; （c）获得指示第二域内偏倚的信息; 以及（d）使用指示第一域内的偏差的信息和指示第二域内的偏差的信息来产生跨越两个域的偏差的指示。

6. 发明授权

US10208335B2 Methods for the analysis of proximity binding assay data 有权
公开(公告)号：US10208335B2
公开(公告)日：2019-02-19
申请号：US12851532
申请日：2010-08-05
申请人： Shiaw-Min Chen , David W. Ruff , Harrison M. Leong
发明人： Shiaw-Min Chen , David W. Ruff , Harrison M. Leong
IPC分类号： G06F19/24 , G06F19/00 , G06F17/00 , C12Q1/6844 , G06F19/20
摘要： Various embodiments of methods for analyzing proximity binding assay (PBA) data are disclosed. Proximity binding assays as a class of analyses offer the advantages of the sensitivity and specificity of biorecognition binding, along with the exponential signal amplification offered by a variety of oligonucleotide amplification reactions, such as the polymerase chain reaction (PCR). However, as various proximity binding assays have reaction kinetics governed by an additional step of the binding of a biorecognition probe (BRP) with a target molecule, there is a need for methods for the analysis of PBA data that are particularly suited to the unique characteristics of such data.

7. 发明授权

US08323897B2 Multiplex amplification of polynucleotides 有权
标题翻译：多核苷酸多重扩增
公开(公告)号：US08323897B2
公开(公告)日：2012-12-04
申请号：US10723520
申请日：2003-11-26
申请人： Mark R. Andersen , David W. Ruff
发明人： Mark R. Andersen , David W. Ruff
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , C12Q1/6806 , C12Q1/6827 , C12Q1/6851 , C12Q2531/113 , C12Q2537/143 , C12Q2561/113 , C12Q2521/107 , C12Q2525/301 , C12Q2561/101
摘要： The present invention provides methods, reagents and kits for carrying out a variety of assays suitable for analyzing polynucleotides or samples that include an amplification step performed in a multiplex fashion. Also provided are methods for analyzing and improving the efficiency of amplification and for carrying out gene expression analysis.
摘要翻译：本发明提供用于进行适于分析多核苷酸或样品的多种测定法的方法，试剂和试剂盒，所述测定包括以多种方式进行的扩增步骤。还提供了用于分析和提高扩增效率和进行基因表达分析的方法。

8. 发明申请

US20110287436A1 Detection Of Analytes And Nucleic Acids 审中-公开
标题翻译：分析物和核酸的检测
公开(公告)号：US20110287436A1
公开(公告)日：2011-11-24
申请号：US13194735
申请日：2011-07-29
申请人： Mark E. Shannon , David W. Ruff
发明人： Mark E. Shannon , David W. Ruff
IPC分类号： C12Q1/68 , C07C291/04 , C07C309/19 , C07C211/63 , C07C309/69 , C07D213/04 , C07J41/00
CPC分类号： C12Q1/6804 , C12Q2563/131 , C12Q2561/125
摘要： Methods of detecting at least one analyte and at least one nucleic acid in a sample are provided. Reagents for carrying out the methods are also provided.
摘要翻译：提供了检测样品中至少一种分析物和至少一种核酸的方法。还提供了用于执行方法的试剂。

9. 发明授权

US08012685B2 Detection of analytes and nucleic acids 有权
标题翻译：检测分析物和核酸
公开(公告)号：US08012685B2
公开(公告)日：2011-09-06
申请号：US11888656
申请日：2007-07-31
申请人： Mark E. Shannon , David W. Ruff
发明人： Mark E. Shannon , David W. Ruff
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6804 , C12Q2563/131 , C12Q2561/125
摘要： Methods of detecting at least one analyte and at least one nucleic acid in a sample are provided. Reagents for carrying out the methods are also provided.
摘要翻译：提供了检测样品中至少一种分析物和至少一种核酸的方法。还提供了用于执行方法的试剂。

10. 发明申请

US20110208441A1 Methods for the Analysis of Proximity Binding Assay Data 审中-公开
标题翻译：接近结合测定数据分析方法
公开(公告)号：US20110208441A1
公开(公告)日：2011-08-25
申请号：US12851532
申请日：2010-08-05
申请人： Shiaw-Min Chen , David W. Ruff , Harrison M. Leong
发明人： Shiaw-Min Chen , David W. Ruff , Harrison M. Leong
IPC分类号： G06F19/00
CPC分类号： C12Q1/6844 , G06F19/20 , G06F19/24 , C12Q2545/114
摘要： Various embodiments of methods for analyzing proximity binding assay (PBA) data are disclosed. Proximity binding assays as a class of analyses offer the advantages of the sensitivity and specificity of biorecognition binding, along with the exponential signal amplification offered by a variety of oligonucleotide amplification reactions, such as the polymerase chain reaction (PCR). However, as various proximity binding assays have reaction kinetics governed by an additional step of the binding of a biorecognition probe (BRP) with a target molecule, there is a need for methods for the analysis of PBA data that are particularly suited to the unique characteristics of such data.
摘要翻译：公开了用于分析邻近结合测定（PBA）数据的方法的各种实施方案。作为一类分析的接近结合测定提供了生物识别结合的灵敏度和特异性以及由多种寡核苷酸扩增反应（例如聚合酶链反应（PCR））提供的指数信号扩增的优点。然而，由于各种邻近结合测定具有由生物识别探针（BRP）与靶分子结合的附加步骤所决定的反应动力学，因此需要分析特别适合于独特特征的PBA数据的方法的数据。

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