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    • 116. 发明申请
    • SYSTEM AND METHOD FOR DETERMINING THE SIZES AND QUANTITY OF POLYNUCLEOTIDES WITH CAPILLARY ARRAY ELECTROPHORESIS
    • 用毛细管阵列电泳法测定多晶硅的尺寸和数量的系统和方法
    • WO2004033621A2
    • 2004-04-22
    • PCT/US2003/014389
    • 2003-05-01
    • SPECTRUMEDIX LLC
    • LIU, ZhaoweiGUO, ZhiyongLI, QingboKANE, Thomas
    • C12N
    • G01N27/44782C12Q1/6816C12Q2565/125C12Q2545/113C12Q2545/101
    • An electrophoretic separation system configured to determine a size of each of a plurality of sample polynucleotides includes a plurality of sample separation lanes, such as capillaries. Each separation lane is configured to subject a number plurality of respective sample polynucleotides and a respective internal standard polynucleotide (ISP) to electrophoresis. The system also includes a ladder separation lane for subjecting at least two members of polynucleotide ladder to electrophoresis. A processor of the system is configured to determine migration coordinates of (1) the ISP and sample polynucleotides subjected to electrophoresis within each separation lane and (2) at least two of the PLMs. The processor also transforms the migration coordinates of the sample polynucleotides of each separation lane from a migration dimension of their respective separation lane to a migration dimension of the polynucleotide ladder. The sizes of the sample polynucleotides are determined based on (1) the respective transformed migration coordinates thereof and (2) migration coordinates of at least two PLMs.
    • 配置成确定多个样品多核苷酸中的每一个的大小的电泳分离系统包括多个样品分离通道,例如毛细管。 每个分离通道被配置为使多个相应的样品多核苷酸和相应的内标多核苷酸(ISP)经受电泳。 该系统还包括用于使多核苷酸梯度的至少两个成员进行电泳的梯子分离通道。 系统的处理器被配置为确定(1)在每个分离通道内经受电泳的ISP和样品多核苷酸的迁移坐标和(2)至少两个PLM。 处理器还将每个分离通道的样品多核苷酸的迁移坐标从它们各自的分离通道的迁移维度转变为多核苷酸梯度的迁移维度。 基于(1)各自的变换迁移坐标和(2)至少两个PLM的迁移坐标来确定样品多核苷酸的大小。
    • 118. 发明申请
    • MICROFLUIDIC ANALYTICAL APPARATUS
    • 微流感分析仪
    • WO2003052821A1
    • 2003-06-26
    • PCT/US2002/041651
    • 2002-12-06
    • ACLARA BIOSICENCES, INC.BJORNSON, TorleifMAHER, Kevin
    • BJORNSON, TorleifMAHER, Kevin
    • H01L23/48
    • B01L9/527B01J2219/00783B01J2219/00804B01J2219/0081B01J2219/0095B01L2200/02B01L2200/025B01L2300/0816B01L2400/0415G01N27/44704G01N27/44782G01N27/44791H01L2924/0002H05K3/222H01L2924/00
    • A microfluidic analytical apparatus (10) comprising a microfluidic card pairing (12) with a corresponding circuit card (14) and the circuit card pairing (12) with corresponding conductive fingers (16). The microfluidic card (12) has a plurality of channels (32) and ports (54) on a top surface of the card in a desired configuration, the ports (54) in fluid communication with the channels (32). The circuit card (14) has a plurality of conductive pins (60) projecting from a bottom surface of the card and having a configuration that corresponds to the particular configuration of the ports (54) of the microfluidic card (12) that it is paired with. The circuit card (14) is received within a holder (20) that provides that provides multiple functions. Conductive pads (18) are disposed on a top surface of the circuit card (14), the pads (18) in electrical communication with the conductive pins (60) and corresponding to a configuration of conductive fingers (16) connected to a power source to provide voltage to the microfluidic card (12) for analytical operations. Additionally, detection difficulties associates with non-uniformities present on the card surface are overcome using a detection system incorporating a compliantly mounted microscopic head.
    • 一种微流体分析装置(10),包括具有对应的电路卡(14)的微流控卡配对(12)和具有对应导电指状物(16)的电路卡配对(12)。 微流控卡(12)具有在卡的顶表面上具有所需配置的多个通道(32)和端口(54),所述端口(54)与通道(32)流体连通。 电路卡(14)具有从卡的底表面突出的多个导电引脚(60),并且具有与其配对的微流控卡(12)的端口(54)的特定配置相对应的配置 用。 电路卡(14)被接收在提供多个功能的保持器(20)内。 导电焊盘(18)设置在电路卡(14)的顶表面上,焊盘(18)与导电引脚(60)电连通并对应于连接到电源的导电指状物(16)的结构 以向微流控卡(12)提供电压用于分析操作。 此外,使用包含柔性安装的微观头的检测系统来克服与卡表面上存在的非均匀性相关联的检测困难。