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101. 发明专利

AU2005321344A1 Method for producing polyunsaturated long-chain fatty acids in transgenic organisms 未知
公开(公告)号：AU2005321344A1
公开(公告)日：2006-07-06
申请号：AU2005321344
申请日：2005-12-20
申请人： BASF PLANT SCIENCE GMBH
发明人： HEINZ ERNST , BAUER JORG , CIRPUS PETRA , KIRSCH JELENA , ABBADI AMINE
IPC分类号： C12N9/10 , A01K67/027 , C12N5/10 , C12N15/54

102. 发明专利

BRPI0409209A 未知
公开(公告)号：BRPI0409209A
公开(公告)日：2006-03-28
申请号：BRPI0409209
申请日：2004-04-06
申请人： BASF PLANT SCIENCE GMBH
发明人： CIRPUS PETRA , BAUER JOERG , MEYER ASTRID , HEINZ ERNST , ZOHRINGER ULRICH
IPC分类号： C12N9/02 , C12N15/82 , C12N15/00 , C12P7/64 , A01H1/00 , A01K67/00 , A23D9/00 , A23K1/16
摘要： The present invention relates to an improved process for the specific preparation of unsaturated omega-3 fatty acids, and to a process for preparing triglycerides having an increased content of unsaturated fatty acids, particularly of omega-3 fatty acids having more than three double bonds. The invention relates to the preparation of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, having increased content of fatty acids, oils or lipids having Delta-4 double bonds owing to the expression of a Delta-4-desaturase from Euglena gracilis. The invention additionally relates to expression cassettes comprising a nucleic acid sequence, a vector and organisms comprising at least one nucleic acid sequence or one expression cassette. The invention additionally relates to unsaturated fatty acids and to triglycerides having an increased content of unsaturated fatty acids and to the use thereof. Fatty acids and triglycerides have a large number of uses in the food industry, in animal nutrition, cosmetics and in the drugs sector. They are suitable for a wide variety of uses depending on whether they are free saturated or unsaturated fatty acids or triglycerides having an increased content of saturated or unsaturated fatty acids.

103. 发明专利

NO20181488A1 Fremgangsmåte for fremstilling av flerumettede fettsyrer i transgene organismer. 未知
公开(公告)号：NO20181488A1
公开(公告)日：2006-02-24
申请号：NO20181488
申请日：2018-11-21
申请人： BASF PLANT SCIENCE GMBH
发明人： HEINZ ERNST , SPERLING PETRA , CIRPUS PETRA , DOMERGUE FREDERIC , ZANK THORSTEN , BAUER JÖRG , QIU ZIAO , VRINTEN PATRICIA , MEYER ASTRID , KIRSCH JELENA , ABBADI AMINE
IPC分类号： A23D9/00 , A23D9/02 , A23L33/115 , C11B1/00 , C11B1/02 , C12N9/02 , C12N9/10 , C12N15/82 , C12P7/64
摘要： Foreliggende oppfinnelse angår en fremgangsmåte for produksjon av flerumettede fettsyrer i en organisme ved innføring i organismen, nukleinsyrer som koder for polypeptider med Δ5-elongase-aktivitet. Fordelaktig kan disse nukleinsyresekvenser uttrykkes i organismen, hvis det passer sammen med ytterligere nukleinsyresekvenser som koder for polypeptider av biosyntese av fettsyre- eller lipid-metabolisme. Spesielt fordelaktig er nukleinsyresekvenser som koder for en Δ6-desaturase-, Δ5-desaturase-, Δ4-desaturase- og/eller Δ6-elongase-aktivitet. Disse desaturaser og elongaser er fordelaktig avledet fra Thalassiosira, Euglena eller Ostreococcus. Oppfinnelsen angår videre en fremgangsmåte for fremstilling av oljer og/eller triacylglycerider med et forhøyet innhold av langkjedede flerumettede fettsyrer. I en foretrukket utførelsesform angår foreliggende oppfinnelse videre en metode for fremstilling av umettede ω3-fettsyrer og en metode for fremstilling av triglycerider med et forhøyet innhold av umettede fettsyrer, spesielt ω3-fettsyrer med mer enn tre dobbeltbindinger. Oppfinnelsen angår dannelse av en transgen organisme, fortrinnsvis en transgen plante eller en transgen mikroorganisme, med et forhøyet innhold av umettet ω3-fettsyrer, oljer eller lipider med ω3-dobbeltbindinger som resultat av ekspresjon av elongaser og desaturaser anvendt ved fremgangsmåten ifølge oppfinnelsen, fordelaktig sammen med ω3-desaturaser, for eksempel en ω3-desaturase fra sopper av familien Pythiaceae så som slekten Phytophthora, for eksempel slekten og arten Phytophthora infestans eller en ω3-desaturase fra alger så som familien Prasinophyceae, for eksempel slekten Ostreococcus, spesifikt slekten og arten Ostreococcus tauri eller diatoméer så som slekten Thalassiosira, spesifikt slekten og arten Thalassiosira pseudonana. Oppfinnelsen angår videre nukleinsyresekvenser, nukleinsyrekonstruksjoner, vektorer og organismer omfattende nukleinsyresekvensene ifølge oppfinnelsen, vektorer omfattende nukleinsyresekvensene og/eller nukleinsyrekontruksjonene og transgene organismer omfattende de ovennevnte nukleinsyresekvenser, nukleinsyrekonstruksjoner og/eller vektorer. En ytterligere del av oppfinnelsen angår oljer, lipider og/eller fettsyrer produsert ved fremgangsmåten ifølge oppfinnelsen og anvendelse av dem. Oppfinnelsen angår videre umettede fettsyrer og triglycerider med et forhøyet innhold av umettede fettsyrer og anvendelse av dem.

104. 发明专利

NO20054372L 未知
公开(公告)号：NO20054372L
公开(公告)日：2005-12-19
申请号：NO20054372
申请日：2005-09-21
申请人： BASF PLANT SCIENCE GMBH
发明人： HEINZ ERNST , ZAHRINGER ULRICH , CIRPUS PETRA , BAUER JORG , MEYER ASTRID
IPC分类号： C12N9/02 , A01H1/00 , A01K67/00 , A23D9/00 , A23K1/16 , C12N15/82 , C12P7/64
摘要： The present invention relates to an improved process for the specific preparation of unsaturated omega-3 fatty acids, and to a process for preparing triglycerides having an increased content of unsaturated fatty acids, particularly of omega-3 fatty acids having more than three double bonds. The invention relates to the preparation of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, having increased content of fatty acids, oils or lipids having Delta-4 double bonds owing to the expression of a Delta-4-desaturase from Euglena gracilis. The invention additionally relates to expression cassettes comprising a nucleic acid sequence, a vector and organisms comprising at least one nucleic acid sequence or one expression cassette. The invention additionally relates to unsaturated fatty acids and to triglycerides having an increased content of unsaturated fatty acids and to the use thereof. Fatty acids and triglycerides have a large number of uses in the food industry, in animal nutrition, cosmetics and in the drugs sector. They are suitable for a wide variety of uses depending on whether they are free saturated or unsaturated fatty acids or triglycerides having an increased content of saturated or unsaturated fatty acids.

105. 发明专利

NO20053149D0 未知
公开(公告)号：NO20053149D0
公开(公告)日：2005-06-28
申请号：NO20053149
申请日：2005-06-28
申请人： BASF PLANT SCIENCE GMBH
发明人： HEINZ ERNST , RENZ ANDREAS , DOMERGUE FREDERIC , ZANK THORSTEN , ABBADI AMINE
IPC分类号： C12N9/10 , C12N15/82 , C12P7/64 , C12N
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.

106. 发明专利

CA2998666A1 METHOD FOR THE PRODUCTION OF MULTIPLY-UNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 未知
公开(公告)号：CA2998666A1
公开(公告)日：2005-02-10
申请号：CA2998666
申请日：2004-07-16
申请人： BASF PLANT SCIENCE GMBH
发明人： ZANK THORSTEN , BAUER JORG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： C12N15/53 , A01H5/00 , A23D9/00 , A23K20/158 , A23L33/115 , A61K8/36 , A61K31/202 , C11B1/02 , C12N5/10 , C12N9/00 , C12N9/02 , C12N9/10 , C12N15/52 , C12N15/82 , C12P7/64
摘要： Producing polyunsaturated fatty acids (I) comprises introducing nucleic acid sequences coding for delta 9 elongase or delta 6 desaturase, delta 8 desaturase or delta 6 elongase, delta 5 desaturase, delta 5 elongase and delta 4 desaturase activity into an organism to produce a transgenic organism whose total lipids include at least 1 wt.% (I). Producing polyunsaturated fatty acids of formula (I) comprises introducing nucleic acid sequences coding for delta 9 elongase or delta 6 desaturase, delta 8 desaturase or delta 6 elongase, delta 5 desaturase, delta 5 elongase and delta 4 desaturase activity into an organism to produce a transgenic organism whose total lipids include at least 1 wt.% of formula (I). [Image] R 1>OH, coenzyme A (thioester), lysophosphatidyl choline, lysophosphatidyl ethanolamine, lysophosphatidyl glycerol, lysophosphatidyl serine, lysophosphatidyl inositol, sphingobase or OCH 2CH(OR 3>)CH 2OR 2>; R 2>H, lysophosphatidyl choline, lysophosphatidyl ethanolamine, lysophosphatidyl glycerol, lysophosphatidyl serine, lysophosphatidyl inositol, (un)saturated 2-24C alkylcarbonyl or CO(CH 2) n(CH=CHCH 2) m(CH 2) pCH 3; R 3>H, (un)saturated 2-24C alkylcarbonyl or CO(CH 2) n(CH=CHCH 2) m(CH 2) pCH 3; n : 2-9; m : 2-6; p : 0 or 3. Independent claims are also included for: (1) oils, lipids or fatty acids produced as above; (2) oil, lipid or fatty acid composition that comprises polyunsaturated fatty acids produced as above and is derived from transgenic plants; (3) isolated nucleic acid sequence coding for a delta 5 elongase polypeptide having one of 6 amino acid sequences given in the specification; (4) isolated nucleic acid sequence that codes for a delta 6 elongase polypeptide and has one of 4 nucleotide sequences given in the specification; (5) isolated nucleic acid sequence that codes for a omega 3 desaturase polypeptide and has one of 2 nucleotide sequences given in the specification; (6) isolated nucleic acid sequence that codes for a delta 6 desaturase polypeptide and has one of 2 nucleotide sequences given in the specification; (7) isolated nucleic acid sequence that codes for a delta 5 desaturase polypeptide and has one of 4 nucleotide sequences given in the specification; (8) isolated nucleic acid sequence that codes for a delta 4 desaturase polypeptide and has one of 2 nucleotide sequences given in the specification; (9) isolated nucleic acid sequence that codes for a delta 12 desaturase polypeptide and has one of 2 nucleotide sequences given in the specification; (10) amino acid sequence encoded by an isolated nucleic acid sequence as above; (11) genetic construct comprising an isolated nucleic acid as above linked to one or more regulatory signals; (12) vector containing a nucleic acid or construct as above; (13) transgenic non-human organism containing a nucleic acid, construct or vector as above.

107. 发明专利

CA2521378A1 .DELTA.-4 DESATURASES FROM EUGLENA GRACILIS, EXPRESSING PLANTS, AND OILS CONTAINING PUFA 未知
公开(公告)号：CA2521378A1
公开(公告)日：2004-10-21
申请号：CA2521378
申请日：2004-04-06
申请人： BASF PLANT SCIENCE GMBH
发明人： CIRPUS PETRA , MEYER ASTRID , BAUER JOERG , HEINZ ERNST , ZAEHRINGER ULRICH
IPC分类号： C12N9/02 , C12N15/82 , A23D9/00 , A01H1/00 , A01K67/00 , C12N15/00 , A23K1/16 , C12P7/64
摘要： The invention relates to an improved method for the specific production of unsaturated .omega.-3 fatty acids and a method for producing triglycerides containing an increased number of unsaturated fatty acids, especially .omega .- 3 fatty acids comprising more than three double bonds. Disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism containing an increased number of fatty acids, oils , or lipids comprising .DELTA.-4 double bonds as a result of a .DELTA.-4 desaturase being expressed from Euglena gracilis. The invention further relates to expression cassettes containing a nucleic acid sequence, a vector , and organisms containing at least one nucleic acid sequence or an expression cassette. Also disclosed are unsaturated fatty acids and triglycerides havin g an increased fatty acid content and the use thereof. Fatty acids and triglycerides are used in a plurality of application in the food industry, i n animal nutrition, cosmetics, and pharmaceuticals and are suitable for many different applications depending on whether free saturated or unsaturated fatty acids or triglycerides having an increased saturated or unsaturated fatty acid content are used.

108. 发明专利

AU2004215705A1 Method for the production of polyunsaturated fatty acids 未知
公开(公告)号：AU2004215705A1
公开(公告)日：2004-09-10
申请号：AU2004215705
申请日：2004-01-29
申请人： BASF PLANT SCIENCE GMBH
发明人： DOMERGUE FREDERIC , ZANK THORSTEN , RENZ ANDREAS , HEINZ ERNST , ABBADI AMINE
IPC分类号： C12N9/10 , C12N15/82 , C12P7/64
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.

109. 发明专利

NO20033268A 未知
公开(公告)号：NO20033268A
公开(公告)日：2003-09-17
申请号：NO20033268
申请日：2003-07-18
申请人： BASF PLANT SCIENCE GMBH
发明人： LERCHL JENS , DUWENIG ELKE , BISCHOFF FRIEDRICH , HEINZ ERNST , DREXLER HJOERDIS , SCHEFFLER JODI
IPC分类号： C12N9/02 , C12N9/10 , C12N15/29 , C12N15/53 , C12N15/54 , C12N15/82 , A01H5/00
CPC分类号： C12N9/0083 , C12N9/1029 , C12N15/8247

110. 发明专利

NO20033239L 未知
公开(公告)号：NO20033239L
公开(公告)日：2003-09-16
申请号：NO20033239
申请日：2003-07-17
申请人： BASF PLANT SCIENCE GMBH
发明人： LERCHL JENS , RENZ ANDREAS , HEINZ ERNST , DOMERGUE FREDERIC , ZAEHRINGER ULRICH
IPC分类号： A01H5/00 , A01K67/027 , A23D9/007 , A23L1/30 , A61K8/30 , A61K8/34 , A61K8/37 , A61K31/201 , A61K31/202 , A61K31/231 , A61K31/232 , A61K31/7088 , A61K36/00 , A61K39/395 , A61K45/00 , A61P3/06 , C07K16/40 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/02 , C12N15/09 , C12N15/53 , C12N15/82 , C12P7/64 , C12Q1/02 , C12Q1/26 , G01N33/15 , G01N33/50 , C12N15/29
摘要： The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, 3, 5, 9, and 11 encoding polypeptides having desaturase or elongase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.

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