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    • 94. 发明申请
    • CHROMOSOMAL LANDING PADS AND RELATED USES
    • 染色线条和相关用途
    • US20120258541A1
    • 2012-10-11
    • US13440661
    • 2012-04-05
    • Vincent P. MauroWei ZhouBruce CunninghamGerald M. Edelman
    • Vincent P. MauroWei ZhouBruce CunninghamGerald M. Edelman
    • C12N15/85C12N5/10C12N15/11
    • C12N15/907C12N2320/32C12N2800/107C12N2800/30
    • Provided herein are methods for stable integration and/or expression of one or more recombinant polynucleotides in a host cell. The recombinant polynucleotides are typically integrated into the host genome at some native chromosomal integration sites. The integration can be mediated by homologous recombination or by using a hybrid recombinase targeting the specific chromosomal locations. The native chromosomal integration sites in the host cells, which support stable integration and strong transcription activities of foreign genes, are present within or adjacent to specific genes in the CHO genome, ankyrin 2 gene (Ank2), cleavage and polyadenylation specific factor 4 gene (Cpsf4), C-Mos gene, and Nephrocystin-1/Mal gene. Also provided are methods and nucleic acid molecules for inserting site-specific recombination sequences (chromosomal landing pads) into these specific chromosomal locations, engineered host cells containing chromosomal landing pads, methods and compositions (e.g., kits) therefore.
    • 本文提供了用于在宿主细胞中稳定整合和/或表达一种或多种重组多核苷酸的方法。 重组多核苷酸通常在某些天然染色体整合位点整合到宿主基因组中。 整合可以通过同源重组或通过使用靶向特定染色体位置的杂交重组酶来介导。 宿主细胞中的天然染色体整合位点,其支持外源基因的稳定整合和强的转录活性,存在于CHO基因组,锚蛋白2基因(Ank2),切割和聚腺苷酸化特异性因子4基因(Ank2) Cpsf4),C-Mos基因和Nephrocystin-1 / Mal基因。 还提供了用于将位点特异性重组序列(染色体着陆垫)插入这些特定染色体位置的方法和核酸分子,因此含有染色体着陆垫的工程化宿主细胞,方法和组合物(例如,试剂盒)。
    • 98. 发明申请
    • SHARING FORM TRAINING RESULT UTILIZING A SOCIAL NETWORK
    • 使用社交网络的共享培训结果
    • US20110282941A1
    • 2011-11-17
    • US12779445
    • 2010-05-13
    • Yuk L. ChanTin Hang ToWei ZhouUman Chan
    • Yuk L. ChanTin Hang ToWei ZhouUman Chan
    • G06F15/16
    • G06Q10/06G06Q50/01
    • A mechanism is provided for sharing form data by users of a social network. A first value input for a first label by a first user is received. The first label is associated with a tag in a mapping table. The first value is associated with the tag in a first user table. The first value is recognized as input for a different label than the first label. The different label is associated with the tag in mapping table. A second value is received that is input for a second label by a second user. The second label is associated with the tag in the mapping table. The second value is associated with the tag in a second user table. The second value is recognized as input for a different label than the second label. The different label than the second label is associated with the tag in mapping table.
    • 提供了一种用于由社交网络的用户共享表单数据的机制。 接收第一用户对第一标签的第一值输入。 第一个标签与映射表中的标签相关联。 第一个值与第一个用户表中的标记相关联。 第一个值被识别为与第一个标签不同的标签的输入。 不同的标签与映射表中的标签相关联。 接收由第二用户输入第二标签的第二值。 第二个标签与映射表中的标签相关联。 第二个值与第二个用户表中的标签相关联。 第二个值被识别为与第二个标签不同的标签的输入。 与第二个标签不同的标签与映射表中的标签相关联。