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1. 发明授权

US5010004A Method for continuous reaction with fluidized immobilized lipase 失效
标题翻译：与流化固定化脂肪酶连续反应的方法
公开(公告)号：US5010004A
公开(公告)日：1991-04-23
申请号：US255599
申请日：1988-10-11
申请人： Yoshitsugu Kosugi , Hideoki Tanaka , Hideo Suzuki , Masaru Shiraki
发明人： Yoshitsugu Kosugi , Hideoki Tanaka , Hideo Suzuki , Masaru Shiraki
IPC分类号： C12P7/64 , C12M1/40
CPC分类号： C12M23/34 , C12M21/18 , C12M23/02 , C12M25/20 , C12M27/00 , C12M47/10 , Y10S435/813
摘要： A water-soluble substrate and an oily substrate are continuously reacted with immobilized lipase in a reaction vessel having vertically maintained apart upper and lower conically-shaped regions, respectively, for separation of a water-soluble product and an oily product, a plurality of lipase reaction zones each containing immobilized lipase capable of being fluidized and an agitating means, and a plurality of intermediate separation zones for separation of an oily substance and a water-soluble substance. The lipase reaction zones and the intermediate separation zones are disposed alternately between the upper and lower conically-shaped separation regions. Boundaries between the lipase reaction zones and intermediate separation zones are pervious to liquid but impervious to the immobilized lipase. The water-soluble substrate and oily substrate are passed in counterflow contact through the lipase reaction zones and intermediate separation zones and mutually contact the immobilized lipase which has been fluidized. An oily product is recovered from the upper conically-shaped separation region and a water-soluble product is recovered from the lower conically-shaped separation region.
摘要翻译：水溶性底物和油性底物分别在具有垂直保持分开的上下圆锥形区域的反应容器中与固定化脂肪酶连续反应，用于分离水溶性产物和油性产物，多种脂肪酶每个含有能够流化的固定化脂肪酶的反应区和搅拌装置，以及用于分离油性物质和水溶性物质的多个中间分离区。脂肪酶反应区和中间分离区交替设置在上下圆锥形分离区之间。脂肪酶反应区和中间分离区之间的边界可以渗透液体，但对固定的脂肪酶是不透水的。水溶性底物和油性底物通过反向流动接触通过脂肪酶反应区和中间分离区，并与已流化的固定化脂肪酶相互接触。从上部圆锥形分离区域回收油状产物，并从下锥形分离区域回收水溶性产物。

2. 发明授权

US5292649A Method for reaction of lipase upon fatty acid 失效
标题翻译：脂肪酸反应脂肪酸的方法
公开(公告)号：US5292649A
公开(公告)日：1994-03-08
申请号：US912655
申请日：1992-07-09
申请人： Yoshitsugu Kosugi , Hideo Suzuki , Akio Sato
发明人： Yoshitsugu Kosugi , Hideo Suzuki , Akio Sato
IPC分类号： C12N11/08 , C12N11/12 , C12N11/14 , C12P7/64 , C12P7/40
CPC分类号： C12P7/6418 , C12N11/08 , C12N11/12 , C12N11/14 , C12P7/6445
摘要： The reaction of a lipase upon a fatty acid is effected by an improved method which comprises bringing the lipase bonded at multiple points to an anion-exchange residue or a carrier and a carrier having a free anion-exchange group admixed therewith into contact with a reaction mixture containing an oily substance including the fatty acid and a water-soluble substance thereby forming an oily product and a water-soluble product in the reaction mixture and subsequently separating the two reaction products.
摘要翻译：脂肪酸对脂肪酸的反应通过一种改进的方法进行，该方法包括使多个点连接的阴离子交换残基或载体的脂肪酶和与其混合的游离阴离子交换基团的载体与反应物接触含有包含脂肪酸的油性物质和水溶性物质的混合物，从而在反应混合物中形成油性产物和水溶性产物，随后分离出两种反应产物。

3. 发明授权

US4013512A Method for adsorption and elution of lipid hydrolyzing enzymes 失效
标题翻译：脂质水解酶的吸附和洗脱方法
公开(公告)号：US4013512A
公开(公告)日：1977-03-22
申请号：US507050
申请日：1974-09-18
申请人： Yoshitsugu Kosugi , Hideo Suzuki , Akira Kamibayashi
发明人： Yoshitsugu Kosugi , Hideo Suzuki , Akira Kamibayashi
IPC分类号： C12N9/20 , C12N11/06 , C12N11/10 , C07G7/02
CPC分类号： C12N9/20 , C12N11/06 , C12N11/10 , Y10S435/815 , Y10S435/874 , Y10S435/921
摘要： A lipid hydrolyzing enzyme-containing substance is brought into contact with an insoluble carrier having a hydrophobic organic compound attached thereto in the form of a hydrophobic residue so that the lipid hydrolyzing enzyme is adsorbed by the hydrophobic residue attached to the insoluble carrier. Then, the lipid hydrolyzing enzyme adsorbed on the carrier is isolated by elution.
摘要翻译：使含脂肪水解酶的物质与具有疏水性残基附着的疏水性有机化合物的不溶性载体接触，使得脂质水解酶被附着于不溶性载体的疏水性残余物吸附。然后，通过洗脱分离吸附在载体上的脂质水解酶。

4. 发明授权

US07401604B2 Engine control device and control method thereof 有权
标题翻译：发动机控制装置及其控制方法
公开(公告)号：US07401604B2
公开(公告)日：2008-07-22
申请号：US11676887
申请日：2007-02-20
申请人： Harumi Furukawa , Yoshitsugu Kosugi
发明人： Harumi Furukawa , Yoshitsugu Kosugi
IPC分类号： F02D41/14
CPC分类号： F02D41/149 , F02D41/1454
摘要： An engine control device and method that prevents fuel injection quantity from becoming unstable due to a rapid change of a correction quantity even in a predetermined operation state such as acceleration time and thus stabilizes an air-fuel ratio. Under a predetermined operating state, even when feedback control of oxygen in exhaust gas is stopped, a correction quantity immediately before the predetermined operating state is held by a correction quantity storage part and the correction of the fuel injection time is performed based on the correction quantity.
摘要翻译：一种发动机控制装置和方法，即使在诸如加速时间的预定操作状态下也能够由于校正量的快速变化而防止燃料喷射量变得不稳定，从而使空燃比稳定。在预定的运行状态下，即使停止排气中的氧的反馈控制，通过校正量存储部保持在预定运行状态之前的修正量，并且基于校正量进行燃料喷射时间的校正。

5. 发明申请

US20070129589A1 Metathesis catalyst and process for producing an olefin using the same 审中-公开
标题翻译：复分解催化剂及使用其制备烯烃的方法
公开(公告)号：US20070129589A1
公开(公告)日：2007-06-07
申请号：US10570317
申请日：2003-09-03
申请人： Masakazu Iwamoto , Yoshitsugu Kosugi
发明人： Masakazu Iwamoto , Yoshitsugu Kosugi
IPC分类号： C07C6/00 , B01J23/00
CPC分类号： B01J29/0341 , B01J23/34 , B01J23/8892 , B01J29/0333 , B01J2229/18 , C07C6/04 , Y02P20/52 , C07C11/107
摘要： A novel olefin production process for forming at least one olefin from two or more olefins in the presence of a catalyst containing at least one selected from the group consisting of nickel, aluminum, manganese, iron and copper which is supported by a regular mesoporous material. In the above olefin production process, propylene can be formed from ethylene and butene. In the above olefin production process, water vapor can be contained in a reaction gas in a molar ratio based on the total of the raw material olefins of 0.001 to 1.
摘要翻译：一种新型烯烃生产方法，用于在含有选自镍，铝，锰，铁和铜中的至少一种的催化剂存在下，由两种或更多种烯烃形成烯烃，该催化剂由常规介孔材料负载。在上述烯烃生产方法中，可以由乙烯和丁烯形成丙烯。在上述烯烃制造方法中，反应气体中可以含有摩尔比为基准的原料烯烃总量为0.001〜1的水蒸气。

6. 发明授权

US6001626A Thermophilic phospholipases and method for production thereof 失效
标题翻译：嗜热磷脂酶及其生产方法
公开(公告)号：US6001626A
公开(公告)日：1999-12-14
申请号：US982312
申请日：1997-12-01
申请人： Yoshitsugu Kosugi , Katsuhiko Higuchi , Kazuhiko Ishikawa , Ikuo Matsui , Joh Yong-Goe
发明人： Yoshitsugu Kosugi , Katsuhiko Higuchi , Kazuhiko Ishikawa , Ikuo Matsui , Joh Yong-Goe
IPC分类号： C12N9/18
CPC分类号： C12Y301/01032 , C12N9/18 , C12Y301/01004
摘要： Disclosed are a thermophilic phospholipase having an optimum temperature range of 95 to 105.degree. C. which is useful in high-temperature degumming processes in oil refining process and high-temperature processing of phospholipids; and a method for producing a thermophilic phospholipase comprising culturing a microorganism capable of producing the phospholipase in a culture medium and collecting the phospholipase from the resultant culture.
摘要翻译：公开了具有95至105℃的最佳温度范围的嗜热磷脂酶，其可用于炼油过程中的高温脱胶过程和磷脂的高温加工; 以及生产嗜热磷脂酶的方法，包括在培养基中培养能够产生磷脂酶的微生物并从所得培养物中收集磷脂酶。

7. 发明授权

US06960454B2 Methods for making and using a thermophilic enzyme as a β-glycosidase 失效
标题翻译：制造和使用嗜热酶作为β-糖苷酶的方法
公开(公告)号：US06960454B2
公开(公告)日：2005-11-01
申请号：US09369735
申请日：1999-08-06
申请人： Ikuo Matsui , Kazuhiko Ishikawa , Hiroyasu Ishida , Yoshitsugu Kosugi
发明人： Ikuo Matsui , Kazuhiko Ishikawa , Hiroyasu Ishida , Yoshitsugu Kosugi
IPC分类号： C12N15/09 , C12N1/21 , C12N9/24 , C12N9/42 , C12N15/00 , C12R1/01 , C12R1/19 , C12P19/04 , C07K1/00 , C12N9/26 , C12P19/02
CPC分类号： C12Y302/01021 , C12N9/2402
摘要： The invention relates to a thermophilic enzyme having β-glycosidase activity which comprises the amino acid sequence of SEQ ID NO: 2 in which one or a plurality of amino acid residues may be deleted, replaced or added.
摘要翻译：本发明涉及具有β-糖苷酶活性的嗜热酶，其包含SEQ ID NO：2的氨基酸序列，其中可以缺失，替换或添加一个或多个氨基酸残基。

8. 发明授权

US06391604B1 Thermostable enzyme having phosphatidylethanolamine N-methyltransferase activity 失效
标题翻译：具有磷脂酰乙醇胺N-甲基转移酶活性的热稳定酶
公开(公告)号：US06391604B1
公开(公告)日：2002-05-21
申请号：US09519729
申请日：2000-03-03
申请人： Ikuo Matsui , Kazuhiko Ishikawa , Hiroyasu Ishida , Yoshitsugu Kosugi , Yasutaka Tahara
发明人： Ikuo Matsui , Kazuhiko Ishikawa , Hiroyasu Ishida , Yoshitsugu Kosugi , Yasutaka Tahara
IPC分类号： C12N910
CPC分类号： C12N9/1007 , C12Y201/01017 , Y10S435/822
摘要： A thermostable phosphatidylethanolamine N-methyltransferase and a process for producing the enzyme are provided. A host cell is transformed with an expression vector containing DNA encoding a thermostable enzyme derived from hyper-thermophilic archaea such as Pyrococcus, the enzyme having phosphatidylethanolamine N-methyltransferase activity and an optimum temperature of 90° C. or higher, and the transformed host cell is cultured.
摘要翻译：提供了热稳定性磷脂酰乙醇胺N-甲基转移酶及其制备方法。宿主细胞用含有编码来源于嗜热古细菌的热稳定酶的DNA的表达载体转化，如Pyrococcus，具有磷脂酰乙醇胺N-甲基转移酶活性的酶，最适温度为90℃或更高，转化的宿主细胞被培养

9. 发明授权

US6133012A Thermostable acyl peptide hydrolase and gene encoding the same 失效
标题翻译：热稳定性酰基肽水解酶和编码基因的基因
公开(公告)号：US6133012A
公开(公告)日：2000-10-17
申请号：US16080
申请日：1998-01-30
申请人： Kazuhiko Ishikawa , Ikuo Matsui , Hiroyasu Ishida , Yoshitsugu Kosugi , Katsuhiko Higuchi
发明人： Kazuhiko Ishikawa , Ikuo Matsui , Hiroyasu Ishida , Yoshitsugu Kosugi , Katsuhiko Higuchi
IPC分类号： C12N15/09 , C07H21/04 , C12N9/48 , C12N9/78 , C12N15/00 , C12Q1/37 , C12R1/01 , C12R1/19 , C12N9/52
CPC分类号： C12N9/48
摘要： An acyl peptide hydrolase having an optimum temperature range of 90-95.degree. C. and a gene encoding the same are disclosed. With the above enzyme, it becomes possible to conduct amino terminal analysis of acylated proteins and peptides at high temperatures.
摘要翻译：公开了具有最佳温度范围为90-95℃的酰基肽水解酶和编码该酰基肽水解酶的基因。利用上述酶，可以在高温下进行酰化蛋白质和肽的氨基末端分析。

10. 发明授权

US06255081B1 Thermostable flap endonuclease derived from hyperthermophile bacterium belonging to the genus pyrococcus 失效
标题翻译：衍生自属于高球菌属的嗜热菌细菌的热稳定性瓣内切酶
公开(公告)号：US06255081B1
公开(公告)日：2001-07-03
申请号：US09175973
申请日：1998-10-21
申请人： Ikuo Matsui , Kazuhiko Ishikawa , Yoshitsugu Kosugi , Eriko Matsui , Satoko Kawasaki
发明人： Ikuo Matsui , Kazuhiko Ishikawa , Yoshitsugu Kosugi , Eriko Matsui , Satoko Kawasaki
IPC分类号： C12P1934
CPC分类号： C12N9/22
摘要： The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.
摘要翻译：本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质的DNA，或（b）具有瓣内切核酸酶活性的蛋白质由氨基酸序列（a），一个或多个氨基酸缺失，取代或添加的氨基酸序列组成。根据本发明，提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。此外，该酶是热稳定的，因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术

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