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1. 发明专利

JPH06153905A CELL CULTURE BASE AND METHOD FOR CELL CULTURE 失效
公开(公告)号：JPH06153905A
公开(公告)日：1994-06-03
申请号：JP33816392
申请日：1992-11-24
申请人： SANYO CHEMICAL IND LTD
发明人： SATO YASUO
IPC分类号： A61K35/407 , C12M1/00 , C12N5/07 , C12N5/071 , C12N5/06 , C12N5/08
摘要： PURPOSE:To obtain a cell culture base which forms a cell aggregate not to cause dedifferentiation for a long period of time, is capable of eliminating floating cell aggregate, prevents necrosis of internal cells caused by aggregation of mutual floating cell aggregates, is capable of making cells cultured in vitro to develop functions of cells in vivo, maintaining the functions for a long period of time, is readily and inexpensively prepared and has high stability and to culture cells. CONSTITUTION:In a method of cell culture, a cell culture base having the surface of the base composed of a vinyl-based copolymer containing 10-90mol% 2-hydroxyethyl methacrylate is used, cell aggregate is formed and cell aggregate is bonded to the surface of the base and maintained to give a cell culture base. Cells are cultured by using the cell culture base.

2. 发明专利

JPH06284883A HEPATOCYTE CELL CULTURE 失效
公开(公告)号：JPH06284883A
公开(公告)日：1994-10-11
申请号：JP20709492
申请日：1992-07-10
申请人： SANYO CHEMICAL IND LTD
发明人： SATO YASUO
IPC分类号： C12M3/00 , C12N5/07 , C12N5/071 , C12N5/06 , C12N5/08
摘要： PURPOSE:To provide a hepatocyte culturing process which can maintain the functions of hepatocytes in an increased level for a prolonged period of time and is suitable for high cell density and large volume culture. CONSTITUTION:This hepatocyte cell culture process comprises that hepatocytes are cultured together with at least one of skin-originating fibroblasts, lung- originating fibroblasts, biliary epithelial cells, sinusoid endothelial cells and vascular endothelial cells in a porous structure having connecting pores where the pore diameter of the porous structure is 1.5 to 100-fold the average diameter of the hepatocytes and the cell culture base material in its inner surfaces of the pores is treated with a substance selected from the group consisting of saccharides, proteins, lipids, synthetic high polymers and their composite materials.

3. 发明专利

JPH07258311A CURABLE COMPOSITION 失效
公开(公告)号：JPH07258311A
公开(公告)日：1995-10-09
申请号：JP7805994
申请日：1994-03-23
申请人： SANYO CHEMICAL IND LTD
发明人： NAKAYAMA HIROMITSU , KATSUKAWA MAKOTO , SATO YASUO , FUJIOKA KOJI
IPC分类号： C08F2/44 , C08F2/48 , C08F2/50 , C08F20/10 , C08F20/28 , C08K3/10
摘要： PURPOSE:To obtain a curable compsn. which has excellent storage stability, causes no discoloration during storage for a long period of time, and is free from the generation of heat and the danger of explosion attributable to the runaway of radical polymerization by incorporating a radical polymerizable compd. and a particular compd. CONSTITUTION:A radical polymerizable compd., pref. an alpha,beta-unsatd. carboxylic acid ester (e.g. trimethylolpropane ethylene oxide (3mol added) triacrylate) and a copper compd. (e.g. copper hydroxide), pref. one or more of org. acid salt, inorg. acid salt aryd hydroxide of copper, are incorporated. Pref., the copper compd. is incorporated in an amount of 10ppb to 100ppm in terms of copper.

4. 发明专利

JPH03236773A SUBSTRATE FOR CULTURING CELL AND METHOD FOR CULTURING CELL 失效
公开(公告)号：JPH03236773A
公开(公告)日：1991-10-22
申请号：JP3295790
申请日：1990-02-14
申请人： SANYO CHEMICAL IND LTD
发明人： SATO YASUO , ANDO SHINYA
IPC分类号： C12M3/00 , C12N5/07 , C12N5/071 , C12N5/10
摘要： PURPOSE:To obtain a substrate which is a cellular structure, having the surface composed of a carbohydrate, protein, lipid, synthetic polymer or complex compounds thereof and capable of culture with maintained highly functional expression of cultured cells for a long period by specifying pore diameter thereof. CONSTITUTION:A substrate which is a cellular structure having the surface composed of any one or more of a carbohydrate, protein, lipid, synthetic polymer and complex compounds thereof and its pore diameter of 1.5-10 times based on that of cells having the largest average diameter in two or more kinds of cells subjected to simultaneous culturing and used for cell culture. The formation of the aforementioned substrate surface can be carried out by a method for dipping the cellular structure in a solution of the above-mentioned substances an then drying the structure or coating the cellular structure with the solution and drying the resultant substrate. In order to provide the substances for forming the substrate surface with stability, the cellular structure can be subjected to treatment with radiation, ozone, ions, chemicals, physical treatment with laser, etc., then dipped or coated or, as necessary, chemically bound to the surface by an active ester, carbodiimide, isocyanate, etc.

5. 发明专利

JPH01259084A HEAT-RESPONDING MATERIAL 失效
公开(公告)号：JPH01259084A
公开(公告)日：1989-10-16
申请号：JP8778588
申请日：1988-04-08
申请人： SANYO CHEMICAL IND LTD
发明人： TANAKA KEIJI , AKAMATSU TAKASHI , JO KIYOKAZU , SATO YASUO
IPC分类号： C08B37/08 , B01J20/26 , C09K5/00
摘要： PURPOSE:To provide the subject material containing an oxyalkylene ether of a polysaccharide as a constituent component, insoluble and non-swellable in water, having water absorption reversibly varying with temperature, contracting with heat to release absorbed water even in the presence of large excess water and useful as a temperature sensor, etc. CONSTITUTION:The objective material is composed of a crosslinked polymer or graft crosslinked polymer of an oxyalkylene ether of a polysaccharide (preferably chitosan) and preferably a vinyl monomer and is useful as a concentrating and separating agent for water-soluble organic substance, a mechanochemical element material, a moisture-controlling agent for toy, an anti-fogging agent, etc.

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