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1. 发明公开

EP0974665A4 RECOMBINANT YEAST PDI AND PROCESS FOR PREPARING THE SAME 失效
标题翻译：重组PDI从酵母和方法及其
公开(公告)号：EP0974665A4
公开(公告)日：2003-02-05
申请号：EP98901535
申请日：1998-02-06
申请人： ORIENTAL YEAST CO LTD
发明人： ISHII NOBUYOSHI , SUZUKI YASUO , UCHIDA KOHJI , MATUO YUSHI , TANAKA HIDEO
IPC分类号： C12N9/90 , C12N15/61 , C12N1/19 , C12N15/81
CPC分类号： C12N9/90 , Y10S435/942
摘要： A process for preparing a biologically active recombinant yeast protein disulfide isomerase (PDI), which comprises: a) modifying a gene encoding the yeast PDI in such a manner that the gene undergoes the deletion, substitution or addition of one or more bases in the region encoding an endoplasmic reticulum localization signal to thereby fail in encoding part or the whole of the localization signal, b) integrating the modified gene into an expression vector, c) transforming host cells with the resultant vector, and d) culturing the transformed host cells in a medium while keeping the pH of the medium near neutrality, thus secreting the PDI in an active state out of the host cells. This process makes it possible to secrete the recombinant yeast PDI in a biologically active state in a large amount in the medium and recover the PDI by a simple and convenient purification method.

2. 发明专利

DE2165406A1 Chromatographic isolation of enzymes - using water-insoluble, (in)org cpds contg orthophosphate gps 未知
公开(公告)号：DE2165406A1
公开(公告)日：1973-07-19
申请号：DE2165406
申请日：1971-12-29
申请人： ORIENTAL YEAST CO LTD
发明人： FUJII KATSUMI , TAKAGAHARA ISAMU , SUZUKI YASUO , HORIO TAKEKAZU
IPC分类号： C12N9/00 , C07G7/03
摘要： Crude enzyme soln. (pref. after removal of basic proteins) is contacted with a water-insoluble, (in)org. cpd contg. orthophosphate gps. (e.g. cellulose phosphate) whereby enzymes related to phosphates (i.e. assimilating or producing phosphates, or influenced in their activity by inhibiting or catalysing action of phosphates) are adsorbed thereon and can be selectively eluted with solns. contg. a competitive eluent, a coenzyme, an inhibitor and/or an activator specific for each enzyme. This method can be applied e...g. to isolation of glycerinaldehyde-3-phosphate-dehydrogenase, pyrophosphatase, glucose-6-phosphate-dehydrogenase, 6-phosphogluconate-dehydrogenase, ribulose-1,6-diphosphate-dehydrogenase, pyruvate-kinase, fumarase and urease.

3. 发明专利

JP2007075032A D-lactate dehydrogenase 有权
标题翻译： D-LACTATE DEHYDROGENASE
公开(公告)号：JP2007075032A
公开(公告)日：2007-03-29
申请号：JP2005268471
申请日：2005-09-15
申请人： Oriental Yeast Co Ltd , オリエンタル酵母工業株式会社
发明人： TAKIZAWA NAOKO , NAKAMURA ATSUO , YASUDA NAOTAKA , SUZUKI YASUO
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/04 , C12Q1/32
摘要： PROBLEM TO BE SOLVED: To provide a D-lactate dehydrogenase protein of a Lactobacillaceae bacterium, and to provide a nucleic acid encoding the protein. SOLUTION: The D-lactate dehydrogenase protein has a natural amino acid sequence whose one or more amino acid residues are replaced by other amino acid residues, has improved storage stability in a solution, and is encoded in a nucleic acid selected from the following group. (a) A nucleic acid encoding a protein containing an amino acid sequence having a specific sequence. (b) A nucleic acid having a specific base sequence. (c) A nucleic acid encoding a protein having a D-lactate dehydrogenase activity and capable of being hybridized with the nucleic acid (a) or (b) under stringent conditions. And (d) a nucleic acid encoding a protein having a D-lactate dehydrogenase activity and containing at least 80% homogenous amino acid sequence as an amino acid sequence having a specific sequence. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供乳酸杆菌科细菌的D-乳酸脱氢酶蛋白质，并提供编码该蛋白质的核酸。解决方案：D-乳酸脱氢酶蛋白具有其一个或多个氨基酸残基被其它氨基酸残基替代的天然氨基酸序列，具有改善的溶液中的储存稳定性，并编码在选自以下的核酸中：以下组。（a）编码含有具有特定序列的氨基酸序列的蛋白质的核酸。（b）具有特定碱基序列的核酸。（c）编码具有D-乳酸脱氢酶活性并能够在严格条件下能够与核酸（a）或（b）杂交的蛋白质的核酸。和（d）编码具有D-乳酸脱氢酶活性并含有至少80％均一的氨基酸序列的蛋白质的核酸作为具有特定序列的氨基酸序列。版权所有（C）2007，JPO＆INPIT

4. 发明专利

JP2004041044A Highly copper-containing yeast and method for producing the same and highly copper-containing yeast homogenization product, and food 有权
公开(公告)号：JP2004041044A
公开(公告)日：2004-02-12
申请号：JP2002201140
申请日：2002-07-10
申请人： Oriental Yeast Co Ltd , オリエンタル酵母工業株式会社
发明人： MASUDA YOSHIFUMI , SHIMADA SHOJI , SUZUKI YASUO
IPC分类号： A23L1/28 , A23L1/30 , A61K36/06 , A61P7/06 , A61P9/00 , C12N1/16
摘要： PROBLEM TO BE SOLVED: To provide a highly copper-containing yeast which contains the copper in a high concentration, is suitable as a food raw material and the like, and does not deteriorate the flavor and the like of foods, when added to the foods, to provide their homogenization product, to provide a method for producing the highly copper-containing yeast, by which the highly copper-containing yeast can be produced in a state that a copper utilization rate is compatible with a yield per sugar (growth rate), and to provide a food containing the highly copper-containing yeast. SOLUTION: This highly copper-containing yeast which has a copper content of ≥500 wt. ppm based on the dry weight of the cells is produced by washing the copper-containing yeast with 25°C ion-exchanged water, until the copper concentration of the washing liquid becomes ≤1 wt. ppm. The highly copper-containing yeast is characterized in that the copper content of a precipitated fraction obtained by homogenizing the copper-containing yeast, until the un-homogenized cells can not be confirmed under an observation with a microscope, obtaining the precipitated fraction, washing the fraction with the 25°C ion-exchanged water, until the copper concentration of the washing liquid becomes ≤1 wt. ppm, is ≥70 wt. ppm based on the dry weight of the cells. The method for producing the highly copper-containing yeast is characterized by subjecting the yeast to fluid culture so that the amount of the added copper is 50 to 450 wt. ppm based on the total amount of the culture liquid. COPYRIGHT: (C)2004,JPO

5. 发明专利

JP2002300861A FOOD CONTAINING YEAST 失效
公开(公告)号：JP2002300861A
公开(公告)日：2002-10-15
申请号：JP2001107174
申请日：2001-04-05
申请人： ORIENTAL YEAST CO LTD
发明人： SHIMADA SHOJI , MASUDA YOSHIFUMI , SUZUKI YASUO
IPC分类号： A23C9/13 , A23L1/28 , A23L1/30 , A23L2/38 , A23L2/52 , A61K36/06 , A61P3/02 , A61K35/72
摘要： PROBLEM TO BE SOLVED: To provide an oral and tubal nutrient composition without generating cohesion and precipitation even when including an yeast containing minerals with high contents to an oral intestinal nutrient or a thick liquid diet as a means for supplying minerals. SOLUTION: This oral and tubal nutrient composition is characterized by comprising a spray-dried product of the yeast containing minerals with high contents which is obtained by crushing the cell of the yeast as a material supplying the minerals so that the ratio of particle having =50%.

6. 发明专利

JP2000245438A NEW EXCELLENT BREAD YEAST 失效
公开(公告)号：JP2000245438A
公开(公告)日：2000-09-12
申请号：JP5613599
申请日：1999-03-03
申请人： NAT FOOD RES , ORIENTAL YEAST CO LTD
发明人： TAKANO HIROYUKI , NAKATOMI YASUO , NAKAJIMA RYOICHI , SUZUKI YASUO
IPC分类号： A21D2/26 , C12N1/16 , C12N1/18
摘要： PROBLEM TO BE SOLVED: To efficiently screen and breed the subject new yeast with high bread dough fermentative power, capable of producing bread with good flavor, in particular good smell by using acetoin as an indicator. SOLUTION: This new yeast having high bread dough fermentative power and/or being capable of producing bread with good flavor, in particular good smell is obtained by the following process: a strain capable of highly producing acetoin is screened from a cultured product afforded through pref. aerobic culture; in particular, such a strain as to be >=600 ppm (pref. >=800 ppm) in the acetoin content of the supernatant of a cultured product afforded by aerobic culture in a modified schultz-Atkin medium for 48 h is screened; preferably, diploid yeast capable of highly producing acetoin are crossbred to create excellent tetraploid bread yeast.

7. 发明专利

JPH0218074B2 失效
公开(公告)号：JPH0218074B2
公开(公告)日：1990-04-24
申请号：JP12888382
申请日：1982-07-26
申请人： ORIENTAL YEAST CO LTD
发明人： YAMAGATA YOSHIKI , FUJITA TAKESHI , SUZUKI YASUO , KOKAWARA ISAMU , FUJII KATSUMI
IPC分类号： C12Q1/32 , C12Q1/26 , C12Q1/34 , C12Q1/58

8. 发明专利

JPS6116022B2 JPS6116022B2 - 失效
公开(公告)号：JPS6116022B2
公开(公告)日：1986-04-26
申请号：JP14535278
申请日：1978-11-27
申请人： Oriental Yeast Co Ltd
发明人： SAITO HIROSHI , SUZUKI YASUO , FUJII KATSUMI , MYAZAKI KO , HORIO BUICHI
IPC分类号： G01N27/26 , G01N1/00 , G01N27/447 , G01N30/04 , G01N33/50 , G01N33/58 , G01N33/68

9. 发明专利

JPS615091A REMOVAL OF NADH INHIBITOR 失效
公开(公告)号：JPS615091A
公开(公告)日：1986-01-10
申请号：JP12362384
申请日：1984-06-18
申请人： ORIENTAL YEAST CO LTD
发明人： FUJITA TAKESHI , YAMAMOTO YOSHIYUKI , YAMAGATA YOSHIKI , SUZUKI YASUO , KOUKAWARA ISAMU , FUJII KATSUMI
IPC分类号： C07H19/207
摘要： PURPOSE:Nicotinamide adenine dinucleotide (NADH) containing its inhibitors is brought into contact with NAD -depending enzyme to effect high-efficiency removal of the inhibitors through simple operations. CONSTITUTION:NADH containing its inhibitors is brought into contact with at least one of NAD -depending enzymes such as lactic acid dehydrogenase (LDH), preferably in 50-200mM concentration buffer solution of 7.5-9.5pH such as phosphoric acid, tris-hydrochloric acid at 2-10 deg.C to effect the adsorption of the inhibitors to the enzyme to give NADH free from inhibitors. The NAD -depending enzyme is preferably immobilized on a support such as cellulose or porous glass and used in the form of a column filled therewith.

10. 发明专利

JPS5931700A Determination of organism substance producing ammonia as reaction product 失效
标题翻译：作为反应产物的生产氨基酸的有机物质的测定
公开(公告)号：JPS5931700A
公开(公告)日：1984-02-20
申请号：JP14050182
申请日：1982-08-14
申请人： Oriental Yeast Co Ltd
发明人： YAMAGATA YOSHIKI , FUJITA TAKESHI , SUZUKI YASUO , KOUKAWARA ISAMU , FUJII KATSUMI
IPC分类号： G01N33/50 , C12Q1/32 , C12Q1/34 , C12Q1/58
摘要： PURPOSE: To determine directly an organism substance producing ammonia as a reaction product, exisiting in a specimen containing ammonia, by using a combination of specific enzymatic reaction systems.
CONSTITUTION: (i) Glutamic dehydrogenase (GlDH), α-ketoglutaric acid (α- KG), reductive nicotinamide adenine dinucleotide (NADH), and an enzyme and substrate [e.g., isocitric dehydrogenase (iCDH), and isocitric acid] to reduce nicotinamide adenine dinuleotide (NAD
+ ) are added to a specimen, all NH
3 existing in a mixed solution is consumed, and simultaneously, formed NAD
+ is converted into NADH. (ii) Reductive nicotinamide adenine dinucletode phosphate (NADPH) and an enzyme (e.g., urease in case of urea determination) to form NH
3 as a reaction product are added to the mixed solution to carry out the reaction, and the reduction in amount of NADPH is measured to determine an organism substance (e.g., urea) producing NH
3 as a reaction product.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：通过使用特定的酶反应体系的组合，直接测定产生氨作为反应产物的生物物质，含有氨样品。构成：（i）谷氨酸脱氢酶（GlDH），α-酮戊二酸（alpha-KG），还原性烟酰胺腺嘌呤二核苷酸（NADH）以及酶和底物[例如异柠檬酸脱氢酶（iCDH）和异柠檬酸]以还原烟酰胺将腺嘌呤二核苷酸（NAD +）加入到样品中，所有存在于混合溶液中的NH 3被消耗，同时形成的NAD +被转化为NADH。（ii）在混合溶液中加入还原性烟酰胺腺嘌呤二核苷酸（NADPH）和酶（例如尿素测定的情况下的脲酶）形成NH 3作为反应产物进行反应，并且还原NADPH 测量产生NH 3作为反应产物的生物物质（例如尿素）。

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