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    • 10. 发明专利
    • HARVEST OF FISSION YEAST POLYPLOID STRAIN
    • JPH0919284A
    • 1997-01-21
    • JP18974895
    • 1995-07-04
    • ORIENTAL YEAST CO LTD
    • HAMADA KAZUHIRONAKATOMI YASUOSHIMADA SHOJI
    • C12N15/00C12N1/16C12N15/01C12R1/645
    • PROBLEM TO BE SOLVED: To obtain a fission yeast polyploid strain simply, accurately and in high frequency by treating a fission yeast such as Schizosaccharomyces pombe under pressurization, culturing the fission yeast on an agar medium containing a pigment and selecting polyploid colonies based on a color change. SOLUTION: This harvest of a fission yeast polyploid strain comprises inoculating the fission yeast such as Schizosaccharomyces pombe JY1 strain to a medium, shaking the medium at 30 deg.C for 30 hours, centrifuging the culture solution to collect the yeast, washing the yeast with 0.86% NaCl aqueous solution, suspending the yeast in 0.86% NaCl aqueous solution, enclosing the yeast suspension in a polyethylene container, treating the yeast under 100-250Mpa hydrostatic pressure at 25 deg.C for 10 minutes using a pressure treating device, culturing the fission yeast after the heat treatment on an agar medium containing a pigment and selecting the polyploid colonies based on a color change. Thus, a screening can be carried out simply and accurately and the polyploid strain of the objective fission yeast can be effectively obtained in an extremely high frequency.