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1. 发明专利

JPH0542275B2 失效
公开(公告)号：JPH0542275B2
公开(公告)日：1993-06-28
申请号：JP2843884
申请日：1984-02-20
申请人： ORIENTAL YEAST CO LTD
发明人： FUJITA TAKESHI , YAMAGATA YOSHIKI , KOKAWARA ISAMU , FUJII KATSUMI
IPC分类号： G01N33/50 , C12Q1/34 , C12Q1/40

2. 发明专利

JPH0452117B2 失效
公开(公告)号：JPH0452117B2
公开(公告)日：1992-08-20
申请号：JP4143684
申请日：1984-03-06
申请人： ORIENTAL YEAST CO LTD
发明人： FUJITA TAKESHI , YAMAMOTO YOSHUKI , YAMAGATA YOSHIKI , SUZUKI YASUO , KOKAWARA ISAMU , FUJII KATSUMI
IPC分类号： C12P19/32

3. 发明专利

JPS643479B2 失效
公开(公告)号：JPS643479B2
公开(公告)日：1989-01-20
申请号：JP288979
申请日：1979-01-17
申请人： ORIENTAL YEAST CO LTD
发明人： MIZUNO DENICHI , MIKAWA USHIO , SATO SHUNJI , ABE SHIGERU , YAMAZAKI MASATOSHI , ECHIGO AKIRA , SAEKI TAKAMICHI , YAMAGATA YOSHIKI , OOHASHI MINORU
IPC分类号： C12P19/04 , A61K31/70 , A61P35/00 , C12R1/85

4. 发明专利

JPS60186297A REMOVAL OF NAD+ ACTIVATION INHIBITOR 失效
公开(公告)号：JPS60186297A
公开(公告)日：1985-09-21
申请号：JP4143684
申请日：1984-03-06
申请人： ORIENTAL YEAST CO LTD
发明人： FUJITA TAKESHI , YAMAMOTO YOSHIYUKI , YAMAGATA YOSHIKI , SUZUKI YASUO , KOUKAWARA ISAMU , FUJII KATSUMI
IPC分类号： C12P19/32
摘要： PURPOSE:NAD containing substance inhibiting NAD activity is brought into contact with NAD -dependent enzymes in a buffer solution to remove the substance, whereby high-purity and high-activity NAD is obtained which can be used in clinical assays. CONSTITUTION:In the preparation of nicotin amide-adenine dinucleotide (NAD ) the NAD powder containing NAD activity inhibitors is dissolved in a phosphate buffer to adjust its pH to 6.0. Then, an NAD -depending enzyme (lactic acid dehydrogenase) is added to effect reactions and the reaction mixture is subjected to ultrafiltration to remove the NAD -depending enzyme to give the objective high-activity NAD free from inhibitors.

5. 发明专利

JPS6043399A MEASUREMENT OF AMYLASE ACTIVITY 失效
公开(公告)号：JPS6043399A
公开(公告)日：1985-03-07
申请号：JP14964583
申请日：1983-08-18
申请人： ORIENTAL YEAST CO LTD , MEITO SANGYO KK
发明人： YAMAGATA YOSHIKI , KOBAYASHI YOSHINORI , FUJITA TAKESHI , AZUMA TOSHIHIKO , SUZUKI YASUO , MACHIDA HARUO , KOUKAWARA ISAMU , IWASAKI SHINJIROU
IPC分类号： G01N33/66 , C12Q1/32 , C12Q1/40
摘要： PURPOSE:To measure directly amylase activity from a test specimen containing amylase, by converting glucose and/or maltose to be an obstacle for measuring directly amylase activity into glucono-delta-lactone, etc. in the test specimen. CONSTITUTION:Maltose and/or glucose already existing in a test specimen is converted with an alkalophilic glucose dehydrogenase and/or maltose dehydrogenase, showing activity in a basic range, slightly showing activity approximately in a neutral range, into glucono-delta-lactone and/or glucose-glucono-delta-lactone in an alkalie range. The treated substance is then restored to a neutral pH, and amylase activity is directly measured by using an excess amount of one or more selected from starch, dextrin, etc. or its derivative as a substrate.

6. 发明专利

JPS5931697A Pretreatment of specimen 失效
标题翻译：样本预处理
公开(公告)号：JPS5931697A
公开(公告)日：1984-02-20
申请号：JP14050082
申请日：1982-08-14
申请人： Oriental Yeast Co Ltd
发明人： YAMAGATA YOSHIKI , FUJITA TAKESHI , SUZUKI YASUO , KOUKAWARA ISAMU , FUJII KATSUMI
IPC分类号： G01N33/50 , C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/58 , G01N33/48
摘要： PURPOSE: In heating the desired substance in a specimen (urine, blood, etc.) in an ammonia formation system at a constant temperature, to eliminate ammonia existing in the specimen and to enable determination with high accuracy easily, by pretreating the specimen using a specific enzymatic reaction system.
CONSTITUTION: Glutamic dehydrogenase (GlDH), 2-oxoglutaric acid (α-KG), reductive nicotinamide adenine dinucleotide (NADH), and an enzyme and substrate [e.g., isocitric dehydrogenase (iCDH) and isocitric acid] to reduce nicotinamide adenine dinucleotide (NAD
+ ) are added to a specimen, (i) NH
3 existing in a mixed solution is treated with GlDH, α-KG, and NADH, converted into glutamic acid and water, and eliminated, simultaneously, (ii) NAD
+ prepared in this reaction is treated with the enzyme to reduce NAD
+ and converted into NADH.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：在恒温下在氨形成系统中加热试样（尿液，血液等）中所需的物质，以消除样品中存在的氨，并能容易地以高精度进行测定，通过使用特异性酶反应体系。构成：谷氨酸脱氢酶（GlDH），2-氧戊二酸（α-KG），还原性烟酰胺腺嘌呤二核苷酸（NADH）以及酶和底物[例如异柠檬酸脱氢酶（iCDH）和异柠檬酸]以还原烟酰胺腺嘌呤二核苷酸（i）将混合溶液中存在的NH 3用GlDH，α-KG和NADH处理，转化为谷氨酸和水，同时除去（ii）NAD + 在该反应中制备的酶用酶处理以还原NAD +并转化为NADH。

7. 发明专利

JPS5931696A Determination of creatinine 失效
标题翻译： CREATININE的测定
公开(公告)号：JPS5931696A
公开(公告)日：1984-02-20
申请号：JP14050382
申请日：1982-08-14
申请人： Oriental Yeast Co Ltd
发明人： YAMAGATA YOSHIKI , FUJITA TAKESHI , SUZUKI YASUO , KOUKAWARA ISAMU , FUJII KATSUMI
IPC分类号： G01N33/70 , C12Q1/00 , C12Q1/32 , C12Q1/34 , G01N33/50
摘要： PURPOSE: To determine directly creatinine existing in a specimen containing ammonia, by using a combination of specific enzymatic reaction systems.
CONSTITUTION: (i) Glutamic dehydrogenase (GlDH), α-oxoglutaric acid (α-OG), reductive nicotinamide adenine dinucleotide phosphate (NADPH), and an enzyme and substrate [e.g., glucose-6-phosphate dehydrogenase (G-6-PDH) and glucose- 6-phosphate(G-6-P)] to reduce nicotinamide adenine dinucleotide phosphate (NADP
+ ) are added to a specimen, all NH
3 existing in a mixed solution is consumed, and, simultaneously, formed NADP
+ is converted into NADPH. (ii) Reductive nicotinamide adenine dinucleotide phosphate (NADH) and creatinindeiminase are added to the mixed solution to carry out the reaction, and the reduction in amount of NADH is measured to determine creatinine amount.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：通过使用特定酶反应体系的组合，直接测定含有氨的样本中的肌酸酐。构成：（i）谷氨酸脱氢酶（GlDH），α-氧戊二酸（α-OG），还原性烟酰胺腺嘌呤二核苷酸磷酸（NADPH）和酶和底物[例如葡萄糖-6-磷酸脱氢酶（G-6-PDH ）和葡萄糖-6-磷酸（G-6-P）]以将烟酰胺腺嘌呤二核苷酸磷酸酯（NADP +）还原成标本，所有存在于混合溶液中的NH 3被消耗，同时形成NADP < +>被转换成NADPH。（ii）将还原性烟酰胺腺嘌呤二核苷酸磷酸（NADH）和肌酸酐磷酸酶加入到混合溶液中进行反应，并测量NADH量的减少以测定肌酐量。

8. 发明专利

JP2001211814A AGENT FOR IMPROVING BREAD QUALITY 失效
公开(公告)号：JP2001211814A
公开(公告)日：2001-08-07
申请号：JP2000022628
申请日：2000-01-31
申请人： ORIENTAL YEAST CO LTD
发明人： YAMAGATA YOSHIKI , FUKUHARA HIDEYO , SATO NOBUYOSHI
IPC分类号： A21D8/04 , A21D2/24 , A21D2/26
摘要： PROBLEM TO BE SOLVED: To obtain an agent for improving bread quality which enables a bread having flavor and a palatability both inherent in bread without using an emulsifier and also being made of a frozen dough highly resistant to refrigeration to be produced. SOLUTION: This agent for improving bread quality comprises hemicellulase, glucose oxidase, catalase, mesophilic amylase, ascorbic acid and/or cystine, at least.

9. 发明专利

JPH0218077B2 失效
公开(公告)号：JPH0218077B2
公开(公告)日：1990-04-24
申请号：JP14050282
申请日：1982-08-14
申请人： ORIENTAL YEAST CO LTD
发明人： YAMAGATA YOSHIKI , FUJITA TAKESHI , SUZUKI YASUO , KOKAWARA ISAMU , FUJII KATSUMI
IPC分类号： G01N33/70 , C12Q1/32 , C12Q1/34

10. 发明专利

JPS63177785A PURIFICATION OF ISOCITRIC DEHYDROGENASE 失效
公开(公告)号：JPS63177785A
公开(公告)日：1988-07-21
申请号：JP818987
申请日：1987-01-19
申请人： ORIENTAL YEAST CO LTD
发明人： YAMAGATA YOSHIKI , KAWANISHI NOBUYUKI , KITAHARA TETSUO , KOKAWARA ISAMU
IPC分类号： C12N9/04
摘要： PURPOSE:To efficiently purify isocitric dehydrogenase (ICDH) at high recovery ratio in a short time, by subjecting the ICDH to ion exchange chromatography using a weak acidic cation exchange resin. CONSTITUTION:A solution containing isocitric dehydrogenase (ICDH) originated from an yeast, e.g. the genus Saccharomyces, Candida, etc., and requiring nicotinamide adenine dinucleotide phosphate (NADP) as a coenzyme is brought into contact with a weak acidic cation exchange resin having carboxylic acid groups, e.g., 'Amberlite(R)' IRC-50, 'Amberlite(R)' CG-50, 'Diaion(R)' WK10, WK11, WK20, etc., using a column, etc., to adsorb the above-mentioned enzyme, which is then eluted with a suitable eluent to isolate the aimed enzyme.

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