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1. 发明申请

WO2008148392A1 ENZYME ACTIVITY ASSAY USING ROLLING CIRCLE AMPLIFICATION 审中-公开
标题翻译：使用滚动圆放大器的酶活性测定
公开(公告)号：WO2008148392A1
公开(公告)日：2008-12-11
申请号：PCT/DK2008/050132
申请日：2008-06-03
申请人： IN SITU RCP A/S , LOHMANN, Jakob Schwalbe , STOUGAARD, Magnus , KOCH, Jørn Erland
发明人： LOHMANN, Jakob Schwalbe , STOUGAARD, Magnus , KOCH, Jørn Erland
IPC分类号： C12Q1/68 , C12Q1/25
CPC分类号： C12Q1/6844 , G01N33/57484 , G01N33/6896 , G01N2333/91245 , G01N2500/00 , C12Q2521/307 , C12Q2531/125 , C12Q2521/507 , C12Q2521/519
摘要： The present invention relates to an enzyme activity assay using rolling circle amplification for verifying that a sample contains the enzyme activity in question. Thus, the present invention pertains to a method for determining the presence or absence of one or more enzyme activities involved in circularising a non-circular oligonucleotide probe in a biological sample. Furthermore, the present invention concerns liquid compositions comprising one or more oligonucleotide probes. Within the scope of the present invention is also a composition comprising a liquid composition and a tissue sample, and solid support of one or more oligonucleotides of the present invention. Disclosed is also a microfluidic device with one or more compartments for performing rolling circle amplification events, and a method for correlating one or more rolling circle amplification events. Methods for testing the efficacy of a drug, for diagnosing or prognosing a disease, for treating a disease, or for treating prophylactically a disease is furthermore disclosed.
摘要翻译：本发明涉及使用滚环扩增用于验证样品含有所述酶活性的酶活性测定法。因此，本发明涉及用于确定参与生物样品中的非环状寡核苷酸探针的一个或多个酶活性的存在或不存在的方法。此外，本发明涉及包含一种或多种寡核苷酸探针的液体组合物。在本发明的范围内，也是包含液体组合物和组织样品以及本发明的一种或多种寡核苷酸的固体支持物的组合物。还公开了一种具有一个或多个用于执行滚环放大事件的隔间的微流体装置，以及用于使一个或多个滚动圆放大事件相关联的方法。还公开了用于测试药物的功效，用于诊断或预后疾病，用于治疗疾病或用于预防性治疗疾病的方法。

2. 发明申请

WO2009006907A1 PADLOCK PROBE AMPLIFICATION METHODS 审中-公开
标题翻译： PADLOCK探针放大方法
公开(公告)号：WO2009006907A1
公开(公告)日：2009-01-15
申请号：PCT/DK2008/050174
申请日：2008-07-09
申请人： In Situ RCP A/S , LOHMANN, Jakob Schwalbe , STOUGAARD, Magnus , KOCH, Jørgen Erland
发明人： LOHMANN, Jakob Schwalbe , STOUGAARD, Magnus , KOCH, Jørgen Erland
IPC分类号： C12Q1/68 , C12Q1/25
CPC分类号： G01N33/54366 , C12Q1/25 , C12Q1/6844 , C12Q2521/531 , C12Q2531/125
摘要： The present invention relates to an enzyme activity assay using rolling circle 5 amplification for verifying that a sample contains enzyme activity. The enzyme activity assayed is typically involved in processing of mismatched nucleotides and/or damaged nucleotides in a double stranded nucleic acid. The present invention relates to methods for determining the presence of enzyme activities involved in processing double stranded oligonucleotide. Methods are also directed against determining the presence 10 of nucleotide repair enzyme activities involved in the repair of a circular oligonucleotide. The present invention also relates to liquid compositions and solid support both comprising an oligonucleotide probe. Furthermore, the present invention relates to methods for testing the efficacy of a drug, for diagnosing, prognosing, treating a disease by determining the enzyme activity.
摘要翻译：本发明涉及使用滚圈5扩增用于验证样品含有酶活性的酶活性测定法。测定的酶活性通常参与双链核酸中错配核苷酸和/或受损核苷酸的加工。本发明涉及用于测定涉及加工双链寡核苷酸的酶活性的存在的方法。方法还针对确定涉及修饰圆形寡核苷酸的核苷酸修复酶活性的存在10。本发明还涉及包含寡核苷酸探针的液体组合物和固体支持物。此外，本发明涉及通过测定酶活性来测定药物的功效，用于诊断，预测，治疗疾病的方法。

3. 发明公开

EP2751277A2 ENZYME DETECTION BY MICROFLUIDICS 审中-公开
标题翻译： MIKROFLUIDSYSTEM ZUR ENZYMDETEKTION
公开(公告)号：EP2751277A2
公开(公告)日：2014-07-09
申请号：EP12758395.3
申请日：2012-08-31
申请人： Koch, Jørn, Erland , Stougaard, Magnus , Andersen, Birgitta Ruth , Juul, Sissel , Leong, Kam , Ho, Megan Yi-ping
发明人： ANDERSEN, Felicie F. , KOCH, Jørn Erland , STOUGAARD, Magnus , ANDERSEN, Birgitta Ruth , JUUL, Sissel , LEONG, Kam , HO, Megan Yi-Ping
IPC分类号： C12Q1/00
CPC分类号： C12Q1/6893 , C12Q1/00 , C12Q1/025 , C12Q1/04 , C12Q1/689 , G01N33/573 , Y02A50/58
摘要： Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.
摘要翻译：提供微流体实施的检测酶特别是DNA修饰酶的方法，以及检测细胞或表达所述酶的微生物的方法。通过提供由该酶特异性靶向的核酸底物来检测该酶。

4. 发明公开

EP2702174A2 SYSTEM FOR IDENTIFICATION OF MICROORGANISM AND DETECTION OF INFECTIOUS DISORDER 有权
标题翻译：系统，用于微生物鉴定和检测传染病
公开(公告)号：EP2702174A2
公开(公告)日：2014-03-05
申请号：EP12725607.1
申请日：2012-04-27
申请人： Koch, Jørn, Erland , Stougaard, Magnus , Knudsen, Birgitta, R.
发明人： ANDERSEN, Felicie, F. , KOCH, Jørn, Erland , STOUGAARD, Magnus , KNUDSEN, Birgitta, R.
IPC分类号： C12Q1/68
CPC分类号： G01N33/56905 , C12Q1/533 , C12Q1/689 , C12Q1/6893 , C12Q2600/136 , G01N33/5695 , G01N2333/35 , G01N2333/445 , Y02A50/58
摘要： Methods for the identification of microorganisms or infectious disorders are disclosed, comprising obtaining a suitable sample from sources such as persons, animals, plants, food, water or soil. The methods also comprise providing tailored nucleic acid substrate(s) designed to react with a type 1 topoisomerase from one or more microorganism(s) or infectious agent(s), and incubating said substrate with said sample, or extracts or preparations from the sample, so that the substrate is processed by said topoisomerase if said microorganism(s) or infectious agent(s) is present in the sample. Finally, processed substrates are identified and potentially quantified by one or more of a range of standard molecular biology methods and read-out systems. The identification and potential quantification of microorganisms and infectious agents, including but not limited to Plasmodium falciparum and Mycobacterium tuberculosis, in single or multiplexed formats enable diseases to be diagnosed, treated, or prevented through the identification of potentially harmful organisms. This disclosure includes also follow-up tests or continued monitoring to evaluate responses to treatment or eradication efforts. In a further aspect the technology enables the testing of medical or chemical treatments designed to cure or prevent diseases based upon drugs targeting type 1 topoisomerases. Finally, the reagents and platforms needed for said purposes can be compiled from loose parts or provided as user-friendly kits, potentially enabling home testing for a multitude of microorganisms or infectious agents.

5. 发明申请

WO2013029631A3 ENZYME DETECTION BY MICROFLUIDICS 审中-公开
公开(公告)号：WO2013029631A3
公开(公告)日：2013-03-07
申请号：PCT/DK2012/050327
申请日：2012-08-31
申请人： KOCH, Jørn Erland , STOUGAARD, Magnus , ANDERSEN, Birgitta Ruth , JUUL, Sissel , LEONG, Kam , HO, Megan Yi-Ping , ANDERSEN, Felicie F.
发明人： KOCH, Jørn Erland , STOUGAARD, Magnus , ANDERSEN, Birgitta Ruth , JUUL, Sissel , LEONG, Kam , HO, Megan Yi-Ping , ANDERSEN, Felicie F.
IPC分类号： C12Q1/00
摘要： Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.

6. 发明申请

WO2012149936A3 SYSTEM FOR IDENTIFICATION OF MICROORGANISM AND DETECTION OF INFECTIOUS DISORDER 审中-公开
公开(公告)号：WO2012149936A3
公开(公告)日：2012-11-08
申请号：PCT/DK2012/000056
申请日：2012-04-27
申请人： KOCH, Jørn, Erland , STOUGAARD, Magnus , KNUDSEN, Birgitta, R. , ANDERSEN, Felicie, F.
发明人： KOCH, Jørn, Erland , STOUGAARD, Magnus , KNUDSEN, Birgitta, R. , ANDERSEN, Felicie, F.
IPC分类号： C12Q1/68 , C12Q1/533 , G01N33/50
摘要： Methods for the identification of microorganisms or infectious disorders are disclosed, comprising obtaining a suitable sample from sources such as persons, animals, plants, food, water or soil. The methods also comprise providing tailored nucleic acid substrate(s) designed to react with a type 1 topoisomerase from one or more microorganism(s) or infectious agent(s), and incubating said substrate with said sample, or extracts or preparations from the sample, so that the substrate is processed by said topoisomerase if said microorganism(s) or infectious agent(s) is present in the sample. Finally, processed substrates are identified and potentially quantified by one or more of a range of standard molecular biology methods and read-out systems. The identification and potential quantification of microorganisms and infectious agents, including but not limited to Plasmodium falciparum and Mycobacterium tuberculosis, in single or multiplexed formats enable diseases to be diagnosed, treated, or prevented through the identification of potentially harmful organisms. This disclosure includes also follow-up tests or continued monitoring to evaluate responses to treatment or eradication efforts. In a further aspect the technology enables the testing of medical or chemical treatments designed to cure or prevent diseases based upon drugs targeting type 1 topoisomerases. Finally, the reagents and platforms needed for said purposes can be compiled from loose parts or provided as user-friendly kits, potentially enabling home testing for a multitude of microorganisms or infectious agents.

7. 发明申请

WO2006108423A2 METHODS FOR PRODUCTION OF OLIGONUCLEOTIDES 审中-公开
标题翻译：生产寡核苷酸的方法
公开(公告)号：WO2006108423A2
公开(公告)日：2006-10-19
申请号：PCT/DK2006/050012
申请日：2006-04-10
申请人： AARHUS UNIVERSITET , KOCH, Jørn , STOUGAARD, Magnus , LOHMANN, Jakob Schwalbe
发明人： KOCH, Jørn , STOUGAARD, Magnus , LOHMANN, Jakob Schwalbe
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C12N15/10 , C12Q1/6844 , C12Q2531/125 , C12Q2525/131 , C12Q2537/149 , C12Q2525/301 , C12Q2521/307
摘要： The present invention relates to production of oligonucleotides using rolling circle replication, wherein synthesised multimeric oligonucleotides are reduced to mononucleotides using a nicking cassette. Thus, the invention provides a method for the production of oligonucleotides, enabling efficient amplification of oligonucleotides at lengths up to at least 1000 nucleotides and in high amounts contained within a nicking cassette.
摘要翻译：本发明涉及使用滚环复制制备寡核苷酸，其中合成的多聚寡核苷酸使用切口盒还原成单核苷酸。因此，本发明提供了一种生产寡核苷酸的方法，能够有效扩增长度至多为1000个核苷酸的寡核苷酸，并以高含量包含在切口盒中。

8. 发明申请

WO2006108422A2 METHODS FOR PRODUCTION OF OLIGONUCLEOTIDES 审中-公开
标题翻译：生产寡核苷酸的方法
公开(公告)号：WO2006108422A2
公开(公告)日：2006-10-19
申请号：PCT/DK2006/050011
申请日：2006-04-10
申请人： AARHUS UNIVERSITET , KOCH, Jørn , STOUGAARD, Magnus , LOHMANN, Jakob Schwalbe
发明人： KOCH, Jørn , STOUGAARD, Magnus , LOHMANN, Jakob Schwalbe
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6816 , C12Q2531/125 , C12Q2521/101 , C12Q2521/325 , C12Q2525/307 , C12Q2525/301 , C12Q2521/337 , C12Q2525/155 , C12Q2521/501
摘要： The present invention provides oligonucleotides and methods for efficient detection of target nucleic acids using rolling circle replication. In one aspect, the oligonucleotides of the invention are characteristic in that they can be circularised without an external ligation template. In another aspect, the oligonucleotides of the invention are characteristic in that they can generate a free 3'end of the target nucleic acid necessary for rolling circle replication. The oligonucleotides and detection methods of the invention are useful e.g. as research tool and for diagnosis.
摘要翻译：本发明提供了使用滚动圆复制有效检测靶核酸的寡核苷酸和方法。在一个方面，本发明的寡核苷酸的特征在于它们可以在没有外部连接模板的情况下被循环。另一方面，本发明的寡核苷酸的特征在于它们可产生滚动圆复制所必需的靶核酸的游离的3'末端。本发明的寡核苷酸和检测方法是有用的。作为研究工具和诊断。

9. 发明公开

EP2176421A1 PADLOCK PROBE AMPLIFICATION METHODS 审中-公开
标题翻译：挂锁探空仪AMPLIFIKATIONSVERFAHREN
公开(公告)号：EP2176421A1
公开(公告)日：2010-04-21
申请号：EP08773310.1
申请日：2008-07-09
申请人： IN Situ RCP A/S
发明人： LOHMANN, Jakob Schwalbe , STOUGAARD, Magnus , KOCH, Jørgen Erland
IPC分类号： C12Q1/68 , C12Q1/25
CPC分类号： G01N33/54366 , C12Q1/25 , C12Q1/6844 , C12Q2521/531 , C12Q2531/125
摘要： The present invention relates to an enzyme activity assay using rolling circle 5 amplification for verifying that a sample contains enzyme activity. The enzyme activity assayed is typically involved in processing of mismatched nucleotides and/or damaged nucleotides in a double stranded nucleic acid. The present invention relates to methods for determining the presence of enzyme activities involved in processing double stranded oligonucleotide. Methods are also directed against determining the presence 10 of nucleotide repair enzyme activities involved in the repair of a circular oligonucleotide. The present invention also relates to liquid compositions and solid support both comprising an oligonucleotide probe. Furthermore, the present invention relates to methods for testing the efficacy of a drug, for diagnosing, prognosing, treating a disease by determining the enzyme activity.

10. 发明公开

EP2160472A1 ENZYME ACTIVITY ASSAY USING ROLLING CIRCLE AMPLIFICATION 审中-公开
标题翻译：酶活性试验使用滚环扩增
公开(公告)号：EP2160472A1
公开(公告)日：2010-03-10
申请号：EP08748831.8
申请日：2008-06-03
申请人： IN Situ RCP A/S
发明人： LOHMANN, Jakob Schwalbe , STOUGAARD, Magnus , KOCH, Jørn Erland
IPC分类号： C12Q1/68 , C12Q1/25
CPC分类号： C12Q1/6844 , G01N33/57484 , G01N33/6896 , G01N2333/91245 , G01N2500/00 , C12Q2521/307 , C12Q2531/125 , C12Q2521/507 , C12Q2521/519
摘要： The present invention relates to an enzyme activity assay using rolling circle amplification for verifying that a sample contains the enzyme activity in question. Thus, the present invention pertains to a method for determining the presence or absence of one or more enzyme activities involved in circularising a non-circular oligonucleotide probe in a biological sample. Furthermore, the present invention concerns liquid compositions comprising one or more oligonucleotide probes. Within the scope of the present invention is also a composition comprising a liquid composition and a tissue sample, and solid support of one or more oligonucleotides of the present invention. Disclosed is also a microfluidic device with one or more compartments for performing rolling circle amplification events, and a method for correlating one or more rolling circle amplification events. Methods for testing the efficacy of a drug, for diagnosing or prognosing a disease, for treating a disease, or for treating prophylactically a disease is furthermore disclosed.

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