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1. 发明授权

US06482596B1 Method for measuring antigen concentration 有权
标题翻译：抗原浓度测定方法
公开(公告)号：US06482596B1
公开(公告)日：2002-11-19
申请号：US09313350
申请日：1999-05-18
申请人： Hiroshi Ueda , Teruyuki Nagamune , Hajime Nishimura , Izumi Kumagai , Kouhei Tsumoto , Walter C. Mahoney , Greg Winter , Paula A. Schueler
发明人： Hiroshi Ueda , Teruyuki Nagamune , Hajime Nishimura , Izumi Kumagai , Kouhei Tsumoto , Walter C. Mahoney , Greg Winter , Paula A. Schueler
IPC分类号： G01N3353
CPC分类号： G01N33/6845 , C07K16/18 , G01N33/68 , G01N33/6857
摘要： This invention provides a method for measuring an antigen concentration in a sample, which comprises: preparing VH-domain polypeptide and VL-domain polypeptide of an antibody specific to the antigen; labeling one of the polypeptides with a reporter molecule to form labeled polypeptides, and immobilizing the other polypeptide onto solid-phase to form immobilized polypeptides; contacting the antigen-containing sample and the labeled polypeptides with the solid-phase; and measuring the reporter molecule of the labeled polypeptides bound to the immobilized polypeptides. The present invention permits simpler and quicker sandwich ELISA for measurements of an antigen concentration in high sensitivity.
摘要翻译：本发明提供了一种测定样品中抗原浓度的方法，包括：制备抗原特异性抗体的VH结构域多肽和VL结构域多肽; 用报告分子标记多肽之一以形成标记的多肽，并将另一多肽固定在固相上以形成固定的多肽; 使含抗原的样品和标记的多肽与固相接触; 并测量与固定化多肽结合的标记多肽的报道分子。本发明允许更快和更快速的夹心ELISA来测量高灵敏度的抗原浓度。

2. 发明授权

US5981199A Method for measuring antigen concentration 失效
标题翻译：抗原浓度测定方法
公开(公告)号：US5981199A
公开(公告)日：1999-11-09
申请号：US663922
申请日：1996-06-14
申请人： Hiroshi Ueda , Teruyuki Nagamune , Hajime Nishimura , Izumi Kumagai , Kouhei Tsumoto , Walter C. Mahoney , Greg Winter , Paula A. Schueler
发明人： Hiroshi Ueda , Teruyuki Nagamune , Hajime Nishimura , Izumi Kumagai , Kouhei Tsumoto , Walter C. Mahoney , Greg Winter , Paula A. Schueler
IPC分类号： C07K16/18 , G01N33/68 , G01N33/573 , G01N33/53 , G01N33/543
CPC分类号： G01N33/6845 , C07K16/18 , G01N33/68 , G01N33/6857
摘要： This invention provides a method for measuring an antigen concentration in a sample, which comprises: preparing VH-domain polypeptide and VL-domain polypeptide of an antibody specific to the antigen; labeling one of the polypeptides with a reporter molecule to form labeled polypeptides, and immobilizing the other polypeptides onto solid-phase to form immobilized polypeptides; contacting the antigen-containing sample and the labeled polypeptides with the solid-phase; and measuring the reporter molecule of the labeled polypeptides bound to the immobilized polypeptides. The present invention permits simpler and quicker sandwich ELISA for measurements of an antigen concentration in high sensitivity.
摘要翻译：本发明提供了一种测定样品中抗原浓度的方法，其包括：制备抗原特异性抗体的VH结构域多肽和VL结构域多肽; 用报告分子标记多肽之一以形成标记的多肽，并将其它多肽固定在固相上以形成固定的多肽; 使含抗原的样品和标记的多肽与固相接触; 并测量与固定化多肽结合的标记多肽的报道分子。本发明允许更快和更快速的夹心ELISA来测量高灵敏度的抗原浓度。

3. 发明授权

US07635475B2 Diabody-type bispecific antibody 失效
标题翻译：双抗体型双特异性抗体
公开(公告)号：US07635475B2
公开(公告)日：2009-12-22
申请号：US10642284
申请日：2003-08-18
申请人： Izumi Kumagai , Toshio Kudo , Kouhei Tsumoto , Ryutaro Asano
发明人： Izumi Kumagai , Toshio Kudo , Kouhei Tsumoto , Ryutaro Asano
IPC分类号： A61K39/395 , C12P21/08
CPC分类号： C07K16/2863 , A61K2039/505 , C07K16/2809 , C07K2317/24 , C07K2317/56 , C07K2317/626
摘要： The purpose of the present invention is to provide a diabody-type bispecific antibody, which is characterized by having low immunogenicity and high infiltrating activity into tumor tissues, and by being easily mass-produced at a low cost with use of microorganisms, and by being easily altered in function by means of genetic engineering. The diabody-type bispecific antibody shows a more remarkable effect than the conventional diabody-type bispecific antibodies and chemically synthesized bispecific antibodies even in a very low concentration and in the absence of the super antigen. The present invention is related to a diabody-type bispecific antibody, having a first specificity to a human epidermal growth factor (EGF) receptor and a second specificity to a surface antigen expressed by a cell having phagocytosis or cytotoxic activity, a single-chain polypeptide constituting the antibody or each region contained therein, a nucleic acid encoding the polypeptide, a replicable cloning vector or expression vector comprising the nucleic acid, a host cell transformed with the vector, and a pharmaceutical preparation comprising thereof.
摘要翻译：本发明的目的是提供一种双抗体型双特异性抗体，其特征在于对肿瘤组织具有低的免疫原性和高的渗透活性，并且通过使用微生物容易地以低成本批量生产，并且通过通过遗传工程容易地改变功能。双抗体双特异性抗体比常规的双抗体型双特异性抗体和化学合成的双特异性抗体甚至在非常低的浓度和不存在超抗原的情况下显示出更显着的效果。本发明涉及对人表皮生长因子（EGF）受体具有第一特异性的双抗体型双特异性抗体和由具有吞噬作用或细胞毒活性的细胞表达的表面抗原的第二特异性，单链多肽构成抗体或其中包含的每个区域，编码多肽的核酸，可复制的克隆载体或包含该核酸的表达载体，用该载体转化的宿主细胞及其包含的药物制剂。

4. 发明申请

US20060210564A1 Novel diabody-type bispecific antibody 失效
标题翻译：新型双抗体型双特异性抗体
公开(公告)号：US20060210564A1
公开(公告)日：2006-09-21
申请号：US10642284
申请日：2003-08-18
申请人： Izumi Kumagai , Toshio Kudo , Kouhei Tsumoto , Ryutaro Asano
发明人： Izumi Kumagai , Toshio Kudo , Kouhei Tsumoto , Ryutaro Asano
IPC分类号： A61K39/395 , C07H21/04 , C12P21/06 , C12N15/74 , C12N1/21 , C07K16/24
CPC分类号： C07K16/2863 , A61K2039/505 , C07K16/2809 , C07K2317/24 , C07K2317/56 , C07K2317/626
摘要： The purpose of the present invention is to provide a diabody-type bispecific antibody, which is characterized by having low immunogenicity and high infiltrating activity into tumor tissues, and by being easily mass-produced at a low cost with use of microorganisms, and by being easily altered in function by means of genetic engineering. The diabody-type bispecific antibody shows a more remarkable effect than the conventional diabody-type bispecific antibodies and chemically synthesized bispecific antibodies even in a very low concentration and in the absence of the super antigen. The present invention is related to a diabody-type bispecific antibody, having a first specificity to a human epidermal growth factor (EGF) receptor and a second specificity to a surface antigen expressed by a cell having phagocytosis or cytotoxic activity, a single-chain polypeptide constituting the antibody or each region contained therein, a nucleic acid encoding the polypeptide, a replicable cloning vector or expression vector comprising the nucleic acid, a host cell transformed with the vector, and a pharmaceutical preparation comprising thereof.
摘要翻译：本发明的目的是提供一种双抗体型双特异性抗体，其特征在于对肿瘤组织具有低的免疫原性和高的渗透活性，并且通过使用微生物容易地以低成本批量生产，并且通过通过遗传工程容易地改变功能。双抗体双特异性抗体比常规的双抗体型双特异性抗体和化学合成的双特异性抗体甚至在非常低的浓度和不存在超抗原的情况下显示出更显着的效果。本发明涉及对人表皮生长因子（EGF）受体具有第一特异性的双抗体型双特异性抗体和由具有吞噬作用或细胞毒活性的细胞表达的表面抗原的第二特异性，单链多肽构成抗体或其中包含的每个区域，编码多肽的核酸，可复制的克隆载体或包含该核酸的表达载体，用该载体转化的宿主细胞及其包含的药物制剂。

5. 发明申请

US20130274446A1 FRAGMENT OF HUMANIZED ANTI-EGFR ANTIBODY SUBSTITUTED-LYSINE VARIABLE FRAGMENT AND USE THEREOF 有权
公开(公告)号：US20130274446A1
公开(公告)日：2013-10-17
申请号：US13816420
申请日：2011-07-16
申请人： Izumi Kumagai , Takeshi Nakanishi , Hideaki Sanada , Ryutaro Asano , Mitsuo Umetsu
发明人： Izumi Kumagai , Takeshi Nakanishi , Hideaki Sanada , Ryutaro Asano , Mitsuo Umetsu
IPC分类号： C07K16/28
CPC分类号： C07K16/2863 , C07K2317/24 , C07K2317/56 , C07K2317/622 , C07K2317/626
摘要： The purpose of the present invention is to provide fragments of humanized anti-EGFR antibody substituted-lysine light-chain or heavy-chain variable regions, and single-chain antibodies, etc, comprising the same, having sufficient binding activity (affinity) with target cells, and having the ability to undergo various site-specific and uniform chemical modifications. The present invention pertains to a humanized variable region on the light-chain or heavy-chain of antibody 528 against human epidermal cell growth factor receptor 1 (Her-1), said variable region comprising the amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 4, wherein fragments of humanized anti-EGFR antibody lysine-substituted light-chain variable regions are formed by substituting a different amino acid for all of the lysine residues, or all of the lysine residues except for the lysine residue(s) of one specified moiety.

6. 发明授权

US07871614B2 Gold-binding protein and use thereof 有权
标题翻译：金结合蛋白及其用途
公开(公告)号：US07871614B2
公开(公告)日：2011-01-18
申请号：US12473199
申请日：2009-05-27
申请人： Hidenori Shiotsuka , Takeshi Imamura , Izumi Kumagai
发明人： Hidenori Shiotsuka , Takeshi Imamura , Izumi Kumagai
IPC分类号： G01N33/53 , A61K39/395
CPC分类号： G01N33/532 , C07K16/44 , C07K2317/21 , C07K2317/56 , C07K2317/622 , C07K2317/92
摘要： A protein utilizing an anti-gold antibody and a gold-binding side which is a part of the anti-gold antibody is constructed. This protein is capable of specifically binding to gold. This protein or a complex protein containing such a protein can be used for the detection of a target substance.
摘要翻译：构建了使用抗金抗体和作为抗金抗体的一部分的金结合侧的蛋白质。该蛋白质能够特异性结合金。该蛋白质或含有这种蛋白质的复合蛋白质可用于检测目标物质。

7. 发明申请

US20100173360A1 ZINC OXIDE-BINDING ANTIBODY AND USE THEREOF 审中-公开
标题翻译： ZINC氧化物结合抗体及其用途
公开(公告)号：US20100173360A1
公开(公告)日：2010-07-08
申请号：US12527129
申请日：2008-02-14
申请人： Mitsuo Umetsu , Izumi Kumagai , Ryutaro Asano , Takeshi Nakanishi
发明人： Mitsuo Umetsu , Izumi Kumagai , Ryutaro Asano , Takeshi Nakanishi
IPC分类号： C12P21/02 , C07K16/00 , C12N15/00 , B01J19/00
CPC分类号： C07K16/44 , C07K2317/22 , C07K2318/10 , G01N33/54366
摘要： The present invention relates to a zinc oxide-binding antibody having high stability and binding activity, and high-throughput sensing technology using the antibody, such as biosensors. Specifically, the present invention relates to a peptide-grafted antibody that contains a zinc oxide-recognizing peptide in the CDR H-1 region of a camel antibody, and a solid support (e.g., a biosensor and a protein chip) containing a zinc oxide layer on which the antibody has been immobilized.
摘要翻译：本发明涉及具有高稳定性和结合活性的氧化锌结合抗体以及使用该抗体如生物传感器的高通量感测技术。具体地，本发明涉及在骆驼抗体的CDR H-1区域含有氧化锌识别肽的固体支持物（例如，生物传感器和蛋白质芯片），其含有氧化锌已经固定有抗体的层。

8. 发明申请

US20060115861A1 Structure and method for releasing substance therefrom 失效
公开(公告)号：US20060115861A1
公开(公告)日：2006-06-01
申请号：US11094132
申请日：2005-03-31
申请人： Hidenori Shiotsuka , Takeshi Imamura , Izumi Kumagai
发明人： Hidenori Shiotsuka , Takeshi Imamura , Izumi Kumagai
IPC分类号： G01N33/542 , C12M1/34 , G01N33/53
CPC分类号： A61K9/0009 , A61K9/0097 , G01N33/54313 , Y10S977/70 , Y10S977/705 , Y10S977/721 , Y10S977/722 , Y10S977/83 , Y10S977/84 , Y10S977/904
摘要： A structure comprises at least a porous body holding a substance releasably, comprising a capping member for keeping the substance inside the pore and/or on at least a part of the entire surface of the porous body, and a connecting member for connecting the porous body and the capping member separably, the connecting member comprising a biopolymer compound. A method for releasing a substance from the structure set forth comprises the steps of applying stimulation from outside to the structure, and cleaving at least one of the bonding between the connecting member and the capping member and the bonding between the connecting member and the porous member to make the substance releasable from the structure.

9. 发明授权

US08921525B2 Highly-functional mutant of humanized anti-EGFR antibody variable region 有权
标题翻译：人源化抗EGFR抗体可变区的高功能突变体
公开(公告)号：US08921525B2
公开(公告)日：2014-12-30
申请号：US13510516
申请日：2010-11-11
申请人： Izumi Kumagai , Takeshi Nakanishi , Ryutaro Asano , Mitsuo Umetsu
发明人： Izumi Kumagai , Takeshi Nakanishi , Ryutaro Asano , Mitsuo Umetsu
IPC分类号： C07K16/22 , C07K16/30 , C07K16/28
CPC分类号： C07K16/22 , C07K16/2809 , C07K16/2863 , C07K2317/24 , C07K2317/31 , C07K2317/565 , C07K2317/567 , C07K2317/622 , C07K2317/626 , C07K2317/73 , C07K2317/92
摘要： [Problem]Disclosed is an antibody that exhibits excellent cytotoxicity and cell growth inhibition and that is based on an anti-human epithelial cell growth factor receptor (1) (Her1) antibody (528). Further disclosed is a method for producing same, and the like.[Solution]The mutant of an H chain humanized variable region (5H) or an L chain humanized variable region (5L) of the anti-human epithelial cell growth factor receptor (1) (Her1) antibody (528) is the aforementioned antibody characterized by having one to a plurality (for example: 1 to 5, or 1 to 3) of amino acid mutations within CDR2. Further disclosed are antibody molecules containing said region, a nucleic acid molecule coding for these polypeptides, a method for producing said antibody molecules, and the like.
摘要翻译： [问题]公开了一种表现出优异的细胞毒性和细胞生长抑制的抗体，其基于抗人上皮细胞生长因子受体（1）（Her1）抗体（528）。进一步公开了其制造方法等。 [解决方案]抗人上皮细胞生长因子受体（1）（Her1）抗体（528）的H链人源化可变区（5H）或L链人源化可变区（5L）的突变体是上述抗体通过在CDR2内具有1至多个（例如：1至5个，或1至3个）氨基酸突变。进一步公开的是含有所述区域的抗体分子，编码这些多肽的核酸分子，产生所述抗体分子的方法等。

10. 发明申请

US20090311806A1 GOLD-BINDING PROTEIN AND USE THEREOF 有权
公开(公告)号：US20090311806A1
公开(公告)日：2009-12-17
申请号：US12473203
申请日：2009-05-27
申请人： Hidenori Shiotsuka , Takeshi Imamura , Izumi Kumagai
发明人： Hidenori Shiotsuka , Takeshi Imamura , Izumi Kumagai
IPC分类号： G01N33/553
CPC分类号： G01N33/532 , C07K16/44 , C07K2317/21 , C07K2317/56 , C07K2317/622 , C07K2317/92
摘要： A protein utilizing an anti-gold antibody and a gold-binding side which is a part of the anti-gold antibody is constructed. This protein is capable of specifically binding to gold. This protein or a complex protein containing such a protein can be used for the detection of a target substance.

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