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1. 发明申请

WO1993010251A1 A METHOD FOR OBTAINING PLANTS WITH REDUCED SUSCEPTIBILITY TO PLANT-PARASITIC NEMATODES 审中-公开
标题翻译：一种减少对植物寄生虫的可怕性的植物的方法
公开(公告)号：WO1993010251A1
公开(公告)日：1993-05-27
申请号：PCT/EP1992002559
申请日：1992-11-02
申请人： MOGEN INTERNATIONAL N.V. , SIJMONS, Peter, Christiaan , GODDIJN, Oscar, Johannes, Maria , VAN DEN ELZEN, Peter, J., M. , VAN DER LEE, Frédérique, Marianne
发明人： MOGEN INTERNATIONAL N.V.
IPC分类号： C12N15/82
CPC分类号： C12N9/1029 , C07K14/415 , C12N9/14 , C12N15/8285 , Y02A40/164
摘要： The present invention provides plants which as a result of the expression of a recombinant DNA have reduced susceptibility to a plant-parasitic nematode. According to a preferred embodiment said recombinant DNA causes disruption of a nematode feeding structure. In another preferred embodiment expression of said recombinant DNA is at least retarding formation of a nematode feeding structure. According to a more preferred embodiment said recombinant DNA comprises: 1) a gene-A which upon expression effects disruption of a nematode feeding structure, said gene-A being placed under the control of a promoter-A that drives expression at least in the nematode feeding structure, and 2) a gene-B which upon expression neutralizes the disruptive effect of gene-A, said gene-B being placed under the control of a promoter-B that drives expression in substantially all of the plant's cells wherein gene-A is expressed, with the proviso that said promoter does not effectively drive expression of gene-B in the nematode feeding structure. A preferred gene-A encodes barnase, while gene-B encodes barstar.
摘要翻译：本发明提供作为重组DNA表达的结果的植物对植物寄生线虫的易感性降低。根据优选的实施方案，所述重组DNA导致线虫饲养结构的破坏。在另一个优选的实施方案中，所述重组DNA的表达至少阻止了线虫饲养结构的形成。根据更优选的实施方案，所述重组DNA包含：1）基因-A，其在表达时对线虫饲养结构产生破坏，所述基因-A置于至少在线虫中驱动表达的启动子-A的控制下进食结构，以及2）基因B，其表达中和基因-A的破坏作用，所述基因B被置于启动子-B的控制下，其驱动基本上所有植物细胞中的基因表达，其中基因A 表达，条件是所述启动子不能有效地驱动线虫喂养结构中基因B的表达。优选的基因-A编码芽孢杆菌RNA酶，而基因B编码barstar。

2. 发明申请

WO1992005259A1 TRANSGENIC PLANTS HAVING A MODIFIED CARBOHYDRATE CONTENT 审中-公开
标题翻译：具有改性碳水化合物含量的转基因植物
公开(公告)号：WO1992005259A1
公开(公告)日：1992-04-02
申请号：PCT/NL1991000171
申请日：1991-09-13
申请人： GIST-BROCADES N.V. , MOGEN INTERNATIONAL N.V. , VAN DEN ELZEN, Peter, J., M. , PEN, Jan , HOEKEMA, Andreas , SIJMONS, Peter, Christiaan , VAN OOYEN, Albert, J., J. , RIETVELD, Krijn , QUAX, Wilhelmus, Johannes
发明人： GIST-BROCADES N.V. , MOGEN INTERNATIONAL N.V.
IPC分类号： C12N15/56
CPC分类号： C12N9/2411 , A01H1/00 , C12N9/2408 , C12N9/2414 , C12N9/2417 , C12N9/242 , C12N9/2428 , C12N15/8245
摘要： The present invention provides plants with a modified taste, solids content and/or texture. The invention also provides methods of obtaining such plants via transformation with DNA constructs containing genes encoding enzymes capable of degrading plant polysaccharides and optionally additional genes encoding enzymes which are capable of further modifying the degradation products resulting from the first degradation step.

3. 发明申请

WO1994010320A1 PLANTS WITH REDUCED SUSCEPTIBILITY TO PLANT-PARASITIC NEMATODES 审中-公开
标题翻译：具有减少对植物寄生虫的不利性的植物
公开(公告)号：WO1994010320A1
公开(公告)日：1994-05-11
申请号：PCT/EP1993003091
申请日：1993-11-02
申请人： MOGEN INTERNATIONAL N.V. , SIJMONS, Peter, Christiaan , GODDIJN, Oscar, Johannes, Maria , VAN DEN ELZEN, Petrus, Josephus, Maria , VAN DER LEE, Frederique, Marianne
发明人： MOGEN INTERNATIONAL N.V.
IPC分类号： C12N15/82
CPC分类号： C12Y106/02004 , A01H5/06 , C07K14/415 , C12N9/0008 , C12N9/0042 , C12N9/1029 , C12N9/14 , C12N15/8285 , C12Y102/01009 , Y02A40/164
摘要： The present invention provides recombinant DNA containing a plant expressible gene which comprises in sequence: a promoter that drives expression of a downstream gene specifically in an initital feeding cell and/or a nematode feeding structure, a gene encoding a product that is inhibitory to an endogenous gene encoding a protein or polypeptide selected from the group consisting of ATP synthase, adenine nucleotide translocator, tricarboxylate translocator, dicarboxylate translocator, 2-oxo-glutarate translocator, cytochrome C, pyruvate kinase, glyceraldehyde-3P-dehydrogenase, NADPH-cytochrome p450 reductase, fatty acid synthase complex, glycerol-3P-acyltransferase, hydroxymethyl-glutaryl CoA reductase, aminocyl transferase, a transcription initiation factor, and a transcription elongation factor, a transcription terminator and a polyadenylation signal sequence, and wherein the said gene is expressed in said initial feeding cell or nematode feeding structure upon infection by the said nematode. The invention further provides plasmids, bacterial cells, recombinant plant genomes, as well as plant cells, plants and parts thereof, still harbouring recombinant genomes. The invention further provides plants with reduced susceptibilitiy against plant parasitic nematodes, as well as methods for obtaining same. Growing plants according to the invention in the field reduces yield losses due to nematode attack and/or reduces nematode populations in the soil.
摘要翻译：本发明提供了含有植物可表达基因的重组DNA，其顺序包含：启动子，其特异性地驱动下游基因表达在初始进食细胞和/或线虫饲养结构中，编码抑制内源性产物的基因编码选自ATP合成酶，腺嘌呤核苷酸转运蛋白，三羧酸转运蛋白，二羧酸转运蛋白，2-氧代戊二酸转运蛋白，细胞色素C，丙酮酸激酶，甘油醛-3P脱氢酶，NADPH-细胞色素p450还原酶的蛋白质或多肽的基因，甘油-3P-酰基转移酶，羟甲基 - 戊二酰辅酶A还原酶，氨基转移酶，转录起始因子和转录延伸因子，转录终止子和聚腺苷酸化信号序列，其中所述基因在所述初始饲养细胞或线虫喂养结构在所述ne感染时 matode。本发明还提供了仍含有重组基因组的质粒，细菌细胞，重组植物基因组，以及植物细胞，植物及其部分。本发明还提供对植物寄生线虫具有降低的耐受性的植物，以及获得植物寄生线虫的方法。根据本发明的生长植物在现场减少由线虫攻击引起的产量损失和/或减少土壤中的线虫种群。

4. 发明申请

WO1992017054A1 METHOD FOR THE ISOLATION AND/OR TESTING OF GENES AND PROMOTERS INVOLVED IN PLANT-NEMATODE INTERACTIONS USING PLANTS OF THE GENUS ARABIDOPSIS 审中-公开
标题翻译：在使用植物抗虫药物的植物 - 哺乳动物相互作用中分离和/或测试基因和促进因子的方法
公开(公告)号：WO1992017054A1
公开(公告)日：1992-10-15
申请号：PCT/EP1992000662
申请日：1992-03-24
申请人： MOGEN INTERNATIONAL N.V. , SIJMONS, Peter, Christiaan
发明人： MOGEN INTERNATIONAL N.V.
IPC分类号： A01H01/02
CPC分类号： A01H1/02 , A01H4/00 , C12N15/8237 , C12N15/8239 , C12N15/8285 , Y02A40/164
摘要： The invention provides a method for infecting a root of a plant of the genus Arabidopsis with a plant parasitic nematode under monoxenic conditions, comprising the steps of: (a) contacting the root with a plant parasitic nematode in the presence of a gelling matrix dissolved in a nutrient medium that is substantially free of nematode inhibiting substances, and (b) allowing the nematode to infect the root by forming a feeding structure.
摘要翻译：本发明提供了一种用于在一氧化条件下用植物寄生线虫感染拟南芥属植物的根的方法，包括以下步骤：（a）在溶解于胶体中的胶凝基质存在下使根与植物寄生线虫接触基本上不含线虫抑制物质的营养培养基，和（b）通过形成饲养结构使线虫感染根部。

5. 发明申请

WO2003050283A3 A STRUCTURE FOR PRESENTING DESIRED PEPTIDE SEQUENCES 审中-公开
公开(公告)号：WO2003050283A3
公开(公告)日：2003-06-19
申请号：PCT/NL2002/000810
申请日：2002-12-10
申请人： CATCHMABS B.V. , HOUTZAGER, Erwin , VIJN, Irma, Maria, Caecilia , SIJMONS, Peter, Christiaan
发明人： HOUTZAGER, Erwin , VIJN, Irma, Maria, Caecilia , SIJMONS, Peter, Christiaan
IPC分类号： C12N15/12
摘要： Provided are means, methods for generating binding peptide associated with a suitable core region, the resulting proteinaceous molecule and uses thereof. The invention provides a solution to the problems associated with the use of binding molecules over their entire range of use. Binding molecules can be designed to accommodate extreme conditions of use such as extreme temperatures or pH. Alternatively, binding molecules can be designed to respond to very subtle changes in the environment.

6. 发明申请

WO03050283A2 A STRUCTURE FOR PRESENTING DESIRED PEPTIDE SEQUENCES 审中-公开
标题翻译：用于提供所需的肽序列的结构
公开(公告)号：WO03050283A2
公开(公告)日：2003-06-19
申请号：PCT/NL0200810
申请日：2002-12-10
申请人： CATCHMABS B V , HOUTZAGER ERWIN , VIJN IRMA MARIA CAECILIA , SIJMONS PETER CHRISTIAAN
发明人： HOUTZAGER ERWIN , VIJN IRMA MARIA CAECILIA , SIJMONS PETER CHRISTIAAN
IPC分类号： C12N15/09 , A61K39/395 , A61K47/48 , C07K1/14 , C07K1/22 , C07K16/00 , C07K16/18 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/10 , C12N15/12 , C12P21/02 , G01N33/50
CPC分类号： C07K16/00 , C07K2317/22
摘要： Provided are means, methods for generating binding peptide associated with a suitable core region, the resulting proteinaceous molecule and uses thereof. The invention provides a solution to the problems associated with the use of binding molecules over their entire range of use. Binding molecules can be designed to accommodate extreme conditions of use such as extreme temperatures or pH. Alternatively, binding molecules can be designed to respond to very subtle changes in the environment.
摘要翻译：提供了用于产生与合适的核心区域相关联的结合肽的方法，所得到的蛋白质分子及其用途。本发明提供了在整个使用范围内与使用结合分子相关的问题的解决方案。结合分子可以设计成适应极端的使用条件，如极端的温度或pH值。或者，可以设计结合分子以响应环境中非常微妙的变化。

7. 发明申请

WO2004069211A3 AFFINITY PROTEINS FOR CONTROLLED APPLICATION OF COSMETIC SUBSTANCES 审中-公开
标题翻译：用于控制化妆品应用的亲和性蛋白质
公开(公告)号：WO2004069211A3
公开(公告)日：2004-12-02
申请号：PCT/NL0300876
申请日：2003-12-10
申请人： MABS B V L , HOUTZAGER ERWIN , VIJN IRMA MARIA CAECILIA , SIJMONS PETER CHRISTIAAN , VALINOTTI TONY , MUDGE GRANT , FADEL ADDI
发明人： HOUTZAGER ERWIN , VIJN IRMA MARIA CAECILIA , SIJMONS PETER CHRISTIAAN , VALINOTTI TONY , MUDGE GRANT , FADEL ADDI
IPC分类号： A61K8/64 , A61K8/65 , A61K47/48 , A61Q1/02 , A61Q1/06 , A61Q5/10 , A61Q11/00 , A61Q13/00 , A61Q15/00 , A61Q19/00 , C07K16/18
CPC分类号： A61Q19/00 , A61K8/64 , A61K8/65 , A61K47/6435 , A61K47/6803 , A61K2800/57 , A61Q1/02 , A61Q1/06 , A61Q5/10 , A61Q11/00 , A61Q13/00 , A61Q15/00
摘要： Provided are means and methods for applying cosmetic substance to a desired target. The method comprising providing a conjugate of a proteinaceous substance having a specific affinity for said target molecule linked to a cosmetic substance, whereby the resulting connection between cosmetic substance and target molecule can be disrupted upon the presence of a chemical and/or physical signal.
摘要翻译：提供了将化妆品物质应用于所需目标的方法和方法。所述方法包括提供与化妆品物质连接的所述靶分子具有特异性亲和性的蛋白质物质的缀合物，由此在化学和/或物理信号的存在下，化妆品物质和靶分子之间的所得连接可能被破坏。

8. 发明申请

WO2004069211A2 AFFINITY PROTEINS FOR CONTROLLED APPLICATION OF COSMETIC SUBSTANCES 审中-公开
标题翻译：用于控制化妆品应用的亲和蛋白
公开(公告)号：WO2004069211A2
公开(公告)日：2004-08-19
申请号：PCT/NL2003/000876
申请日：2003-12-10
申请人： L-MABS B.V. , HOUTZAGER, Erwin , VIJN, Irma, Maria, Caecilia , SIJMONS, Peter, Christiaan , VALINOTTI, Tony , MUDGE, Grant , FADEL, Addi
发明人： HOUTZAGER, Erwin , VIJN, Irma, Maria, Caecilia , SIJMONS, Peter, Christiaan , VALINOTTI, Tony , MUDGE, Grant , FADEL, Addi
IPC分类号： A61K7/00
CPC分类号： A61Q19/00 , A61K8/64 , A61K8/65 , A61K47/6435 , A61K47/6803 , A61K2800/57 , A61Q1/02 , A61Q1/06 , A61Q5/10 , A61Q11/00 , A61Q13/00 , A61Q15/00
摘要： Provided are means and methods for applying cosmetic substance to a desired target. The method comprising providing a conjugate of a proteinaceous substance having a specific affinity for said target molecule linked to a cosmetic substance, whereby the resulting connection between cosmetic substance and target molecule can be disrupted upon the presence of a chemical and/or physical signal.
摘要翻译：提供用于将化妆品物质施加到期望的目标的手段和方法。所述方法包括提供对与化妆品物质连接的所述目标分子具有特异性亲和力的蛋白质物质的偶联物，由此在化学物质和/或物理信号的存在下可以破坏化妆品物质与目标分子之间的所得连接。 / p>

9. 发明申请

WO1998022599A1 NEMATODE-INDUCIBLE REGULATORY DNA SEQUENCES 审中-公开
公开(公告)号：WO1998022599A1
公开(公告)日：1998-05-28
申请号：PCT/EP1997006472
申请日：1997-11-18
申请人： MOGEN INTERNATIONAL N.V. , OHL, Stephan, Andreas , VAN DER LEE, Frédérique, Marianne , GODDIJN, Oscar, Johannes, Maria , KLAP, Joke , SIJMONS, Peter, Christiaan
发明人： MOGEN INTERNATIONAL N.V.
IPC分类号： C12N15/82
CPC分类号： A01N65/38 , A01N65/00 , A01N65/08 , C12N15/8239 , C12N15/8285 , Y02A40/164 , A01N2300/00
摘要： The invention provides a DNA fragment obtainable from Arabidopsis thaliana that is capable of promoting root knot and cyst nematode-inducible transcription of an associated DNA sequence when re-introduced into a plant, and the use of said DNA fragment.
摘要翻译：本发明提供从拟南芥获得的DNA片段，其能够在重新引入植物中时促进相关DNA序列的根结和囊状线虫诱导型转录，以及使用所述DNA片段。

10. 发明申请

WO1997046692A1 NEMATODE-INDUCIBLE PLANT GENE PROMOTER 审中-公开
标题翻译： NEMATODE-INDUCIBLE植物基因启动子
公开(公告)号：WO1997046692A1
公开(公告)日：1997-12-11
申请号：PCT/EP1996002437
申请日：1996-06-04
申请人： MOGEN INTERNATIONAL N.V. , OHL, Stephan, Andreas , SIJMONS, Peter, Christiaan , VAN DER LEE, Frédérique, Marianne , GODDIJN, Oscar, Johannes, Maria , KLAP, Joke
发明人： MOGEN INTERNATIONAL N.V.
IPC分类号： C12N15/82
CPC分类号： C12N15/8285 , C07K14/415 , C12N15/8239 , Y02A40/164
摘要： The invention provides DNA fragments obtainable from Arabidopsis thaliana that is capable of promoting root knot and cyst nematode-inducible transcription of an associated DNA sequence when re-introduced into a plant, and the use of said DNA fragments.
摘要翻译：本发明提供了可从拟南芥获得的DNA片段，其能够在重新引入植物中时促进相关DNA序列的根结和囊状线虫诱导型转录，以及所述DNA片段的使用。

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