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1. 发明申请

WO1994010320A1 PLANTS WITH REDUCED SUSCEPTIBILITY TO PLANT-PARASITIC NEMATODES 审中-公开
标题翻译：具有减少对植物寄生虫的不利性的植物
公开(公告)号：WO1994010320A1
公开(公告)日：1994-05-11
申请号：PCT/EP1993003091
申请日：1993-11-02
申请人： MOGEN INTERNATIONAL N.V. , SIJMONS, Peter, Christiaan , GODDIJN, Oscar, Johannes, Maria , VAN DEN ELZEN, Petrus, Josephus, Maria , VAN DER LEE, Frederique, Marianne
发明人： MOGEN INTERNATIONAL N.V.
IPC分类号： C12N15/82
CPC分类号： C12Y106/02004 , A01H5/06 , C07K14/415 , C12N9/0008 , C12N9/0042 , C12N9/1029 , C12N9/14 , C12N15/8285 , C12Y102/01009 , Y02A40/164
摘要： The present invention provides recombinant DNA containing a plant expressible gene which comprises in sequence: a promoter that drives expression of a downstream gene specifically in an initital feeding cell and/or a nematode feeding structure, a gene encoding a product that is inhibitory to an endogenous gene encoding a protein or polypeptide selected from the group consisting of ATP synthase, adenine nucleotide translocator, tricarboxylate translocator, dicarboxylate translocator, 2-oxo-glutarate translocator, cytochrome C, pyruvate kinase, glyceraldehyde-3P-dehydrogenase, NADPH-cytochrome p450 reductase, fatty acid synthase complex, glycerol-3P-acyltransferase, hydroxymethyl-glutaryl CoA reductase, aminocyl transferase, a transcription initiation factor, and a transcription elongation factor, a transcription terminator and a polyadenylation signal sequence, and wherein the said gene is expressed in said initial feeding cell or nematode feeding structure upon infection by the said nematode. The invention further provides plasmids, bacterial cells, recombinant plant genomes, as well as plant cells, plants and parts thereof, still harbouring recombinant genomes. The invention further provides plants with reduced susceptibilitiy against plant parasitic nematodes, as well as methods for obtaining same. Growing plants according to the invention in the field reduces yield losses due to nematode attack and/or reduces nematode populations in the soil.
摘要翻译：本发明提供了含有植物可表达基因的重组DNA，其顺序包含：启动子，其特异性地驱动下游基因表达在初始进食细胞和/或线虫饲养结构中，编码抑制内源性产物的基因编码选自ATP合成酶，腺嘌呤核苷酸转运蛋白，三羧酸转运蛋白，二羧酸转运蛋白，2-氧代戊二酸转运蛋白，细胞色素C，丙酮酸激酶，甘油醛-3P脱氢酶，NADPH-细胞色素p450还原酶的蛋白质或多肽的基因，甘油-3P-酰基转移酶，羟甲基 - 戊二酰辅酶A还原酶，氨基转移酶，转录起始因子和转录延伸因子，转录终止子和聚腺苷酸化信号序列，其中所述基因在所述初始饲养细胞或线虫喂养结构在所述ne感染时 matode。本发明还提供了仍含有重组基因组的质粒，细菌细胞，重组植物基因组，以及植物细胞，植物及其部分。本发明还提供对植物寄生线虫具有降低的耐受性的植物，以及获得植物寄生线虫的方法。根据本发明的生长植物在现场减少由线虫攻击引起的产量损失和/或减少土壤中的线虫种群。

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