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1. 发明申请

WO2007027764A3 NOVEL LINEAR ION TRAP FOR MASS SPECTROMETRY 审中-公开
标题翻译：新型线性离子阱用于质谱分析
公开(公告)号：WO2007027764A3
公开(公告)日：2007-12-13
申请号：PCT/US2006033843
申请日：2006-08-30
申请人： UNIV ROCKEFELLER , KRUTCHINSKY ANDREW N , COHEN HERBERT , CHAIT BRIAN T
发明人： KRUTCHINSKY ANDREW N , COHEN HERBERT , CHAIT BRIAN T
IPC分类号： B01D59/44
CPC分类号： H01J49/423
摘要： A method for manipulating ions in an ion trap includes storing ions, spatially compressing, and ejecting selected ions according to mass-to-charge ratio. An ion trap includes an injection port, an arm having a first and a second end for confining and spatially compressing the ions, and an ejection port for ejecting the ions from the second end. The arm includes two pairs of opposing electrodes, which provide a quadrupole electric field potential at any cross- section of the ion trap. The distance between opposing electrodes and the cross-sectional area of the electrodes increases from the first to second end. The electrodes may be tapered cylindrical rods or of hyperbolic cross-section. Ions selected for ejection are spatially compressed into a region at the second (wider) end. The ion trap may include one arm, with either orthogonal or axial ejection, or two arms with a central insert for orthogonal ejection.
摘要翻译：用于操纵离子阱中的离子的方法包括根据质荷比存储离子，空间压缩和喷射选定的离子。离子阱包括注入口，具有用于限制和空间压缩离子的第一和第二端的臂以及用于从第二端喷射离子的喷射口。该臂包括两对相对的电极，其在离子阱的任何横截面处提供四极电场电势。相对电极之间的距离和电极的横截面积从第一端到第二端增加。电极可以是锥形圆柱形棒或双曲线横截面。选择用于喷射的离子在空间上压缩成第二（较宽）端的区域。离子阱可以包括一个臂，具有正交或轴向喷射，或者两个臂具有用于正交喷射的中心插入物。

2. 发明申请

WO2007022543A3 METHODS AND KITS FOR DISTINGUISHING BETWEEN SPECIFIC AND NON-SPECIFIC PROTEIN ASSOCIATIONS 审中-公开
公开(公告)号：WO2007022543A3
公开(公告)日：2007-02-22
申请号：PCT/US2006/032967
申请日：2006-08-21
申请人： ROCKEFELLER UNIVERSITY , CHAIT, Brian, T. , TACKETT, Alan, J. , DEGRASSE, Jeffrey, A. , OEFFINGER, Marlene , ROUT, Michael, P. , SEKEDAT, Matthew, D.
发明人： CHAIT, Brian, T. , TACKETT, Alan, J. , DEGRASSE, Jeffrey, A. , OEFFINGER, Marlene , ROUT, Michael, P. , SEKEDAT, Matthew, D.
IPC分类号： A61B5/055
摘要： The present invention is method of determining whether or not associations between a given protein and other proteins in a cell are specific. The method comprises (a) providing a first sample of the cells in which the given protein contains a tag, (b) providing a second sample of the same cells, wherein the given protein and the other proteins are metabolically labeled, and wherein neither the given protein nor the other proteins are tagged, (c) mixing and lysing the first cell sample and the second cell sample to provide a mixture of proteins, (d) binding the tag of the given protein to a substrate, (e) isolating proteins associated with the tagged given protein bound to the substrate, whereby the associated proteins comprise: (i) proteins specifically associated with the tagged given protein, (H) proteins non-specifically associated with the tagged given protein, or (Hi) a combination thereof, (f) determining whether each associated protein is unlabeled or a mixture of labeled and unlabeled proteins, wherein if the associated protein is not labeled, then that protein was specifically associated in the cell with the tagged given protein.

3. 发明申请

WO2007022543A2 METHODS AND KITS FOR DISTINGUISHING BETWEEN SPECIFIC AND NON-SPECIFIC PROTEIN ASSOCIATIONS 审中-公开
标题翻译：用于特异性和非特异性蛋白质结合之间鉴别的方法和工具
公开(公告)号：WO2007022543A2
公开(公告)日：2007-02-22
申请号：PCT/US2006032967
申请日：2006-08-21
申请人： UNIV ROCKEFELLER , CHAIT BRIAN T , TACKETT ALAN J , DEGRASSE JEFFREY A , OEFFINGER MARLENE , ROUT MICHAEL P , SEKEDAT MATTHEW D
发明人： CHAIT BRIAN T , TACKETT ALAN J , DEGRASSE JEFFREY A , OEFFINGER MARLENE , ROUT MICHAEL P , SEKEDAT MATTHEW D
IPC分类号： G01N33/567 , G01N33/53 , G01N33/554 , G01N33/569 , G01N33/574
CPC分类号： G01N33/5023 , G01N33/5032 , G01N33/60 , G01N33/6845 , G01N33/6848
摘要： The present invention is method of determining whether or not associations between a given protein and other proteins in a cell are specific. The method comprises (a) providing a first sample of the cells in which the given protein contains a tag, (b) providing a second sample of the same cells, wherein the given protein and the other proteins are metabolically labeled, and wherein neither the given protein nor the other proteins are tagged, (c) mixing and lysing the first cell sample and the second cell sample to provide a mixture of proteins, (d) binding the tag of the given protein to a substrate, (e) isolating proteins associated with the tagged given protein bound to the substrate, whereby the associated proteins comprise: (i) proteins specifically associated with the tagged given protein, (H) proteins non-specifically associated with the tagged given protein, or (Hi) a combination thereof, (f) determining whether each associated protein is unlabeled or a mixture of labeled and unlabeled proteins, wherein if the associated protein is not labeled, then that protein was specifically associated in the cell with the tagged given protein.
摘要翻译：本发明是确定细胞中给定蛋白质和其它蛋白质之间的缔合是否是特异性的方法。该方法包括（a）提供其中给定蛋白质含有标签的细胞的第一样品，（b）提供相同细胞的第二样品，其中所述给定蛋白质和其它蛋白质经代谢标记，并且其中（c）混合和裂解第一细胞样品和第二细胞样品以提供蛋白质的混合物，（d）将给定蛋白质的标签与底物结合，（e）分离蛋白质与标记的给定蛋白质结合至底物，其中相关蛋白质包含：（i）与标记的给定蛋白质特异性相关的蛋白质，（H）与标记的给定蛋白质非特异性相关的蛋白质，或（嗨）其组合，（f）确定每个相关蛋白是否未标记，或标记蛋白和未标记蛋白质的混合物，其中如果相关蛋白未被标记，那么该蛋白质特异性地与具有标记的蛋白质相关联给予蛋白质。

4. 发明申请

WO2007027764A2 NOVEL LINEAR ION TRAP FOR MASS SPECTROMETRY 审中-公开
标题翻译：用于质谱的新型线性离子阱
公开(公告)号：WO2007027764A2
公开(公告)日：2007-03-08
申请号：PCT/US2006/033843
申请日：2006-08-30
申请人： ROCKEFELLER UNIVERSITY , KRUTCHINSKY, Andrew, N. , COHEN, Herbert , CHAIT, Brian, T.
发明人： KRUTCHINSKY, Andrew, N. , COHEN, Herbert , CHAIT, Brian, T.
IPC分类号： B01D59/44
CPC分类号： H01J49/423
摘要： A method for manipulating ions in an ion trap includes storing ions, spatially compressing, and ejecting selected ions according to mass-to-charge ratio. An ion trap includes an injection port, an arm having a first and a second end for confining and spatially compressing the ions, and an ejection port for ejecting the ions from the second end. The arm includes two pairs of opposing electrodes, which provide a quadrupole electric field potential at any cross- section of the ion trap. The distance between opposing electrodes and the cross-sectional area of the electrodes increases from the first to second end. The electrodes may be tapered cylindrical rods or of hyperbolic cross-section. Ions selected for ejection are spatially compressed into a region at the second (wider) end. The ion trap may include one arm, with either orthogonal or axial ejection, or two arms with a central insert for orthogonal ejection.
摘要翻译：用于操作离子阱中的离子的方法包括按照质荷比来存储离子，空间压缩和排出选定的离子。离子阱包括注入口，具有用于限制和空间压缩离子的第一和第二端的臂以及用于从第二端喷射离子的排出口。臂包括两对相对的电极，其在离子阱的任何横截面处提供四极电场电位。相对电极之间的距离和电极的横截面面积从第一到第二端增加。电极可以是锥形圆柱形杆或双曲线截面。选择用于喷射的离子被空间压缩到第二（较宽）端的区域。离子阱可以包括具有正交或轴向排出的一个臂或具有用于正交喷射的中心插入件的两个臂。

5. 发明申请

WO0077712A9 METHOD FOR ASSESSING SIGNIFICANCE OF PROTEIN IDENTIFICATION 审中-公开
标题翻译：评估蛋白质鉴定意义的方法
公开(公告)号：WO0077712A9
公开(公告)日：2002-07-11
申请号：PCT/US0016638
申请日：2000-06-15
申请人： UNIV ROCKEFELLER , ERIKSSON JAN , FENYOE DAVID , CHAIT BRIAN T
发明人： ERIKSSON JAN , FENYOE DAVID , CHAIT BRIAN T
IPC分类号： C07K1/00 , C07K1/04 , G01N33/68 , G06F19/18 , G06F19/00
CPC分类号： G01N33/6848 , C07K1/00 , C07K1/047 , G06F19/18
摘要： A method of generating a frequency distribution of scores for a particular experimental condition, wherein the scores relate to random identifications of biological molecules, the method comprising: a) generating mass data for the particular experimental condition for known biological molecules in a biological molecule database; b) generating mass data of a hypothetical biological molecule for the experimental condition; c) comparing the data generated in step (b) with the data generated for each known biological molecule in step (a); d) calculating a score for each comparison in step (c), wherein the score is a function of similarity between the data generated in step (a), which corresponds to a particular known biological molecule, and the data generated in step (b); e) selecting a score from the scores calculated in step (d), wherein the selected score corresponds to the comparison which denotes a high degree of similarity between the data generated in step (a) and the data generated in step (b); f) repeating steps (b) through (e) with different hypothetical biological molecules until a sufficient quantity of scores are selected; and g) determining the frequency of selecting each score and generating therefrom a frequency distribution of scores.
摘要翻译：一种生成特定实验条件的分数的频率分布的方法，其中所述分数涉及生物分子的随机标识，所述方法包括：a）产生生物分子数据库中已知生物分子的特定实验条件的质量数据; b）为实验条件产生假设生物分子的质量数据; c）将步骤（b）中产生的数据与在步骤（a）中为每个已知生物分子生成的数据进行比较; d）计算步骤（c）中每个比较的得分，其中所述得分是步骤（a）中生成的数据（其对应于特定已知生物分子）与步骤（b）中生成的数据之间的相似性的函数， ; e）从步骤（d）中计算的分数中选择分数，其中所选分数对应于表示步骤（a）中生成的数据与步骤（b）中生成的数据之间的高度相似度的比较; f）用不同的假设生物分子重复步骤（b）至（e），直到选择足够数量的分数; 以及g）确定选择每个分数的频率并由此产生分数的频率分布。

6. 发明申请

WO1993024834A1 METHOD AND PRODUCT FOR THE SEQUENCE DETERMINATION OF PEPTIDES USING A MASS SPECTROMETER 审中-公开
标题翻译：使用质谱仪序列测定肽的方法和产品
公开(公告)号：WO1993024834A1
公开(公告)日：1993-12-09
申请号：PCT/US1993005070
申请日：1993-05-27
申请人： THE ROCKEFELLER UNIVERSITY , CHAIT, Brian, T. , BEAVIS, Ronald , WANG, Rong , KENT, Stephen, B., H.
发明人： THE ROCKEFELLER UNIVERSITY
IPC分类号： G01N33/44
CPC分类号： G01N33/6842 , C07K1/107 , C07K1/128 , G01N33/6824 , Y10T436/24 , Y10T436/25 , Y10T436/255
摘要： Method is described for sequencing polypeptides by forming peptide ladders comprising a series of polypeptides in which adjacent members of the series vary by one amino acid residue and determining the identity and position of each amino acid in the polypeptide by mass spectroscopy.
摘要翻译：描述了通过形成包含一系列多肽的肽梯级来测序多肽的方法，其中该序列的相邻成员变化一个氨基酸残基，并通过质谱确定多肽中每个氨基酸的同一性和位置。

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