基本信息:
- 专利标题： MOLECULAR WEIGHT INTERNAL STANDARD AND APPLICATIONS THEREOF
- 申请号：EP16838600.1 申请日：2016-08-26
- 公开(公告)号：EP3348642A1 公开(公告)日：2018-07-18
- 发明人： BI, Wanli , LU, Weihua
- 申请人： Nuhigh Biotechnologies Co., Ltd
- 申请人地址： Suite 301 C8 218 Xinghu Road Suzhou Industrial Park Suzhou, Jiangsu 215123 CN
- 专利权人： Nuhigh Biotechnologies Co., Ltd
- 当前专利权人： Nuhigh Biotechnologies Co., Ltd
- 当前专利权人地址： Suite 301 C8 218 Xinghu Road Suzhou Industrial Park Suzhou, Jiangsu 215123 CN
- 代理机构： V.O.
- 优先权： CN201510535029 20150827
- 国际公布： WO2017032342 20170302
- 主分类号： C12N15/11
- IPC分类号： C12N15/11 ; C12Q1/68
摘要：
Provided is a molecular weight internal standard, consisting of n nucleotide fragments that have different molecular weights and identifiable markers, the n nucleotide fragments comprising at least p nucleotide fragment pairs differing by one base, and one fragment of the nucleotide fragment pair differing by one base having m bases, and the other fragment having m+1 or m-1 bases; p is 1 to n/2 when n is an even number and p is 1 to (n-1)/2 when n is an odd number. Compared with current commercial molecular weight internal standards, the molecular weight internal standard can be used to identify a single base and differentiate PCR product fragments differing by one base, and is applicable to STR analysis, sequencing, large fragment analysis, and genome analysis.