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1. 发明申请

WO2013068572A2 BLOOD SAMPLE ASSAY METHOD 审中-公开
标题翻译：血液样品测定方法
公开(公告)号：WO2013068572A2
公开(公告)日：2013-05-16
申请号：PCT/EP2012072336
申请日：2012-11-09
申请人： AXIS SHIELD ASA
发明人： FAAREN ARNE LUDVIG , FRANTZEN FRANK , NORDHEI ARNE KRISTIAN , SUNDREHAGEN ERLING , OERNING LARS
IPC分类号： G01N33/92 , C12Q1/60 , C12Q1/61
CPC分类号： C12Q1/60 , C12Q1/61 , G01N33/92
摘要： The invention prov ides an enzymatic method for measuring the concentration of one or more analytes in the plasma portion of a blood derived sample, containing a first and a second component, where said second component interferes with the measurement of said first component. The method includes: i) diluting the sample with a reagent mixture; ii) substantially removing blood cells; iii) using a reagent which serves to temporarily prevent reaction of the second component, to generate a blocked second component; iv) causing the selective reaction of a constituent of each analyte to directly or indirectly generate detectable reaction products, where one of the analytes is the first component; v) monitoring the detectable reaction product or products; vi) relating an amount of the detectable product or products and/or a rate of formation of the detectable product or products to the concentration of each analyte, where the concentration of at least the first component is related to a corresponding detectable reaction product by means of estimating an un-measurable (fictive) endpoint. Step iii) may be carried out at any stage up to and including step iv) but before steps v) or vi). The reagent of step iii) may be applied to the sample separately or may be included in a reagent mixture during steps i) or iv). A corresponding kit is also provided.
摘要翻译：本发明提供了一种酶法，用于测量含有第一和第二组分的血液衍生样品的血浆部分中一种或多种分析物的浓度，其中所述第二组分干扰所述第一组分的测量。该方法包括：i）用试剂混合物稀释样品; ii）基本上除血细胞; iii）使用用于暂时防止第二组分反应的试剂，产生封闭的第二组分; iv）引起每种分析物的组分的选择性反应直接或间接产生可检测的反应产物，其中一种分析物是第一组分; v）监测可检测的反应产物或产物; vi）将可检测产物或产物的量和/或可检测产物或产物的形成速率与每种分析物的浓度相关联，其中至少第一组分的浓度与相应的可检测反应产物相关估计一个不可测量（虚构的）端点。步骤iii）可以在直到并包括步骤iv），但在步骤v）或vi）之前的任何阶段进行。步骤iii）的试剂可以单独施用于样品，或者可以在步骤i）或iv）期间包含在试剂混合物中。还提供了相应的套件。

2. 发明申请

WO2008152380A2 LIPOPROTEIN SURFACTANT 审中-公开
标题翻译： LIPOPROTEIN表面活性剂
公开(公告)号：WO2008152380A2
公开(公告)日：2008-12-18
申请号：PCT/GB2008/001996
申请日：2008-06-11
申请人： OXFORD BIOSENSORS LIMITED , MURPHY, Lindy, Jane
发明人： MURPHY, Lindy, Jane
IPC分类号： C12Q1/60 , C12Q1/61 , C12Q1/00
CPC分类号： C12Q1/004 , C12Q1/60 , C12Q1/61
摘要： A sensor for determining the amount of triglyceride and/or cholesterol in a sample, the sensor comprising: (a) a surfactant of formula (1) wherein each of R a , R b , R c , R d and R e is independently -OH, C 1 -C 4 alkoxy or a group of formula -OCONH(CH 2 ) m -CH 3 , -OCO(CH 2 ) m CH 3 , O(CH 2 ) m -CH 3 , -S(CH 2 ) m -CH 3 , -O(CH 2 ) n -A, -S(CH 2 ) n -A, -OCO(CH 2 ) m -CH 3 or -NHCO(CH 2 ) m -CH 3 , wherein m is from 4 to 20, m' is from 4 to 20, m" is from 4 to 6 or from 8 to 20, n is from 0 to 10 and A is a C 3 -C 8 cycloalkyl group or a phenyl group, with the proviso that at least one of the groups R a , R b , R c , R d and R e is not -OH or C 1 -C 4 alkoxy; and (b) an enzyme reagent for measuring triglycerides and/or an enzyme reagent for measuring cholesterol.
摘要翻译：用于测定样品中甘油三酯和/或胆固醇量的传感器，所述传感器包括：（a）式（1）的表面活性剂，其中Ra，Rb，Rc，Rd和Re各自独立地是-OH，C1-C4 烷氧基或式-OCONH（CH 2）m -CH 3，-OCO（CH 2）m CH 3，O（CH 2）m -CH 3，-S（CH 2）m -CH 3，-O（CH 2）n A，-S（ CH2）nA，-OCO（CH2）m-CH3或-NHCO（CH2）m-CH3，其中m为4至20，m'为4至20，m“为4至6或8至20 n为0至10，A为C 3 -C 8环烷基或苯基，条件是Ra，Rb，Rc，Rd和Re中的至少一个不是-OH或C 1 -C 4烷氧基; 和（b）用于测量甘油三酯的酶试剂和/或用于测量胆固醇的酶试剂。

3. 发明申请

WO2006111238A1 METHOD FOR SCREENING MTP-INHIBITING COMPOUNDS 审中-公开
标题翻译：筛选MTP抑制化合物的方法
公开(公告)号：WO2006111238A1
公开(公告)日：2006-10-26
申请号：PCT/EP2006/002647
申请日：2006-04-12
申请人： MERCK PATENT GMBH , CHEVREUIL, Olivier , DUPONT, Hervé , GUERRIER, Daniel
发明人： CHEVREUIL, Olivier , DUPONT, Hervé , GUERRIER, Daniel
IPC分类号： C12Q1/60 , C12Q1/61
CPC分类号： C12Q1/60 , C12Q1/61 , G01N2500/04 , G01N2500/10
摘要： The present invention relates to a screening method for selecting active materials that inhibit microsomal triglyceride transfer protein (MTP) and to a screening kit using the said method.
摘要翻译：本发明涉及选择抑制微粒体甘油三酯转运蛋白（MTP）的活性物质和使用所述方法的筛选试剂盒的筛选方法。

4. 发明申请

WO02006832A1 MEANS OF EXAMINING NEPHROPATHY 审中-公开
标题翻译：检查NEPHROPATHY的手段
公开(公告)号：WO02006832A1
公开(公告)日：2002-01-24
申请号：PCT/JP2001/006006
申请日：2001-07-11
IPC分类号： C12Q1/25 , C12Q1/26 , C12Q1/32 , C12Q1/44 , C12Q1/48 , C12Q1/60 , C12Q1/61 , G01N33/53 , G01N33/68 , G01N33/92
CPC分类号： G01N33/53 , C12Q1/25 , C12Q1/26 , C12Q1/32 , C12Q1/44 , C12Q1/485 , C12Q1/60 , C12Q1/61 , G01N33/6896 , G01N33/92 , G01N2333/775
摘要： A convenient means of examining nephropathy is provided. Paying attention to fat granules contained in urinary lipomicrons and oval fat which are observed in association with nephropathy, it is found out that the contents of lipoproteins or analogs thereof or constituents of the same contained in these substances significantly indicate relevance to nephropathy, thereby completing the above-described examination means.
摘要翻译：提供了检查肾病的便利手段。注意与肾病相关的尿液脂质体和椭圆形脂肪中含有的脂肪颗粒，发现这些物质中含有的脂蛋白或其类似物或其组分的含量显着表示与肾病的相关性，从而完成上述检查手段。

5. 发明申请

WO0109610A8 METHOD TO MEASURE ACYL-COENZYME A ESTERS 审中-公开
标题翻译：测量酰基 - 辅酶A酯的方法
公开(公告)号：WO0109610A8
公开(公告)日：2001-07-19
申请号：PCT/GB0002061
申请日：2000-05-30
申请人： UNIV GLASGOW , GRAHAM IAN ALEXANDER , LARSON TONY ROBERT
发明人： GRAHAM IAN ALEXANDER , LARSON TONY ROBERT
IPC分类号： C12Q1/00 , C12Q1/61 , G01N33/573 , G01N33/92
CPC分类号： G01N33/92 , C12Q1/00 , C12Q1/61 , G01N33/573 , Y10S435/968 , Y10S435/975
摘要： The invention provides a fluorescent enzymatic method for measuring a acyl coenzyme A (acyl CoA) ester or esters in a sample, comprising eight steps including the use of a derivatizing agent. Also disclosed is a kit for use with the assay of the present invention.
摘要翻译：本发明提供了用于测量样品中的酰基辅酶A（酰基CoA）酯或酯的荧光酶法，其包括八个步骤，包括使用衍生剂。还公开了与本发明的测定法一起使用的试剂盒。

6. 发明申请

WO1997038317A1 METHOD OF MEASURING BILIRUBIN 审中-公开
标题翻译：测量胆红素的方法
公开(公告)号：WO1997038317A1
公开(公告)日：1997-10-16
申请号：PCT/US1997005642
申请日：1997-04-04
申请人： BECKMAN INSTRUMENTS, INC.
发明人： BECKMAN INSTRUMENTS, INC. , SCHULTZ, Cecilia, Z. , YEIN, Fredrick, S.
IPC分类号： G01N33/72
CPC分类号： C12Q1/54 , C12Q1/26 , C12Q1/60 , C12Q1/61 , C12Q1/62 , G01N33/728 , Y10S435/81
摘要： An assay for detecting in a test sample, an analyte that undergoes auto-oxidation is disclosed. The assay comprises the steps of forming a reaction mixture by combining in an aqueous medium (i) a sample containing the analyte and (ii) a stabilizer that reduces the rate of radical mediated auto-oxidation of the analyte. The reaction mixture is incubated for a period of time and the rate of auto-oxidation is detected. When the detected rate of auto-oxidation is substantially zero, a sufficient quantity of an enzyme is added, that catalyzes the oxidation of the analyte. In the presence of the stabilizer, the analyte can be oxidized to a product species. The product species, the analyte, or both can be detected.
摘要翻译：公开了一种用于在测试样品中检测经自动氧化的分析物的测定法。该测定包括以下步骤：通过在水性介质（i）含有分析物的样品和（ii）降低分析物自由基介导的自动氧化速率的稳定剂中混合形成反应混合物。将反应混合物温育一段时间，并检测自身氧化速率。当检测到的自氧化速率基本上为零时，加入足量的酶，催化分析物的氧化。在稳定剂存在下，分析物可被氧化成产物。可以检测产物种类，分析物或二者。

7. 发明申请

WO1994014977A1 HUMAN CYCLOOXYGENASE-2cDNA AND ASSAY FOR EVALUATING INHIBITION OF CYCLOOXYGENASE-2 审中-公开
标题翻译：人CYCLOOXYGENASE-2cDNA和测定CYCLOOXYGENASE-2的抑制作用
公开(公告)号：WO1994014977A1
公开(公告)日：1994-07-07
申请号：PCT/CA1993000547
申请日：1993-12-17
申请人： MERCK FROSST CANADA INC. , CROMLISH, Wanda, A. , KENNEDY, Brian, P. , O'NEILL, Gary , VICKERS, Philip, J. , WONG, Elizabeth , MANCINI, Joseph, A.
发明人： MERCK FROSST CANADA INC.
IPC分类号： C12Q01/00
CPC分类号： C12N9/0083 , C12Q1/48 , C12Q1/61 , C12Y114/99001 , G01N33/5005 , G01N33/88 , G01N2333/90241
摘要： The invention discloses an assay for measuring inhibition of cyclooxygenase-2 in comparison with cyclooxygenase-1. The invention also comprises a human cyclooxygenase-2 cDNA and a human cyclooxygenase-2.
摘要翻译：本发明公开了与环加氧酶-1相比测定环加氧酶-2的抑制作用。本发明还包括人环加氧酶-2 cDNA和人环加氧酶-2。

8. 发明申请

WO2007113336A3 FUNCTIONAL EXPRESSION OF TRIACYLGLYCEROL LIPASES 审中-公开
标题翻译：三酰甘油脂肪酶功能表达
公开(公告)号：WO2007113336A3
公开(公告)日：2008-05-02
申请号：PCT/EP2007053416
申请日：2007-04-05
申请人： BASF SE , HAUER BERNHARD , HAERING DIETMAR , BRANNEBY CECILIA , RUSNAK MONIKA , LIU DANNI , SCHMID ROLF
发明人： HAUER BERNHARD , HAERING DIETMAR , BRANNEBY CECILIA , RUSNAK MONIKA , LIU DANNI , SCHMID ROLF
IPC分类号： C12N9/20 , C12N5/10 , C12Q1/44 , C12Q1/61
CPC分类号： C12N9/20 , C12Q1/44 , C12Q1/61 , G01N2333/918
摘要： The present invention relates to nucleic acids encoding triacylglycerol lipases, to vectors comprising such nucleic acids, to host cells comprising such nucleic acids or vectors, to methods of expressing triacylglycerol lipases in prokaryotes, to methods of detecting and producing triacylglycerol lipases, to triacylglycerol lipases which can be obtained in this manner, and to the use of triacylglycerol lipase-encoding nucleic acids, vectors and recombinant host cells for the abovementioned methods.
摘要翻译：本发明涉及的核酸编码的三酰基甘油脂肪酶，例如核酸，其包含载体，宿主细胞包含此类核酸或载体，方法为在原核生物中的三酰基甘油脂肪酶，用于检测方法的表达和生产的三酰基甘油脂肪酶的可获得从而甘油三酯脂肪酶，以及使用的编码脂酶的三酰甘油，核酸，载体和重组宿主细胞对于所提到的方法。

9. 发明申请

WO2005113797A3 METHODS FOR DETECTING Lp-PLA2 ACTIVITY AND INHIBITION OF Lp-PLA2 ACTIVITY 审中-公开
标题翻译：检测Lp-PLA2活性和抑制Lp-PLA2活性的方法
公开(公告)号：WO2005113797A3
公开(公告)日：2006-06-22
申请号：PCT/US2005012948
申请日：2005-04-14
申请人： GLAXO GROUP LTD , SHOU YAPING , WALKER GEORGE T , SIU YIN-FAI
发明人： SHOU YAPING , WALKER GEORGE T , SIU YIN-FAI
IPC分类号： C12Q1/44 , C12Q1/34 , C12Q1/61
CPC分类号： C12Q1/61 , C12Q1/34 , C12Q1/44 , C12Q2334/10 , G01N2333/918
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
摘要翻译：本发明涉及用于测定来自动物的至少一种样品中Lp-PLA2的活性的方法。本发明还涉及用于测定来自施用Lp-PLA2抑制剂的动物的样品中Lp-PLA2活性的抑制的方法。

10. 发明申请

WO03054516A2 DEVICES AND METHODS FOR ISOLATING AND DETECTING SPECIFIC SUBSTANCES IN COMPLEX MATRICES 审中-公开
标题翻译：用于分离和检测复合矩阵中特定物质的装置和方法
公开(公告)号：WO03054516A2
公开(公告)日：2003-07-03
申请号：PCT/US0240991
申请日：2002-12-20
申请人： SAFETEST INC
发明人： GORDON VIRGINIA C
IPC分类号： G01N33/53 , B01L3/00 , C12N15/09 , C12Q1/02 , C12Q1/44 , C12Q1/61 , C12Q1/68 , G01N1/34 , G01N30/00 , G01N33/02 , G01N33/543 , G01N
CPC分类号： G01N33/543 , B01L3/50255 , C12Q1/61 , C12Q1/6806 , G01N1/34 , G01N33/02 , G01N2001/4016 , C12Q2565/137
摘要： Methods and systems for determining the presence of one or more analytes in various types of matricies,such as foods, oils, biological fluids, etc. Membranes are used to remove extraneous matter (e.g., solid particles) and/or to bind specific interferents or analytes. Specific assays are provided for determining citric acid, total fat (or % fat), free fatty acids, protein content and specific nucleic acid content indicative of the presence of DNA and/or RNA of certain microbes (e.g., pathogens) or genetically altered substances (e.g., genetically engineered grain).
摘要翻译：用于确定各种类型的成分如食物，油，生物液体等中一种或多种分析物的存在的方法和系统。膜用于除去外来物质（例如固体颗粒）和/或结合特定的干扰物或分析物。提供特异性测定法用于测定柠檬酸，总脂肪（或脂肪），游离脂肪酸，蛋白质含量和指示某些微生物（例如病原体）或遗传改变物质的DNA和/或RNA的存在的特定核酸含量（例如，转基因粮食）。

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