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    • 8. 发明申请
    • IMPROVED SMB PROCESS
    • 改进SMB过程
    • WO2013005052A1
    • 2013-01-10
    • PCT/GB2012/051597
    • 2012-07-06
    • BASF PHARMA (CALLANISH) LIMITEDKELLIHER, AdamMORRISON, AngusOROSKAR, AnilNAIR REMA, Rakesh VikramanAGARWAL, Abhilesh
    • KELLIHER, AdamMORRISON, AngusOROSKAR, AnilNAIR REMA, Rakesh VikramanAGARWAL, Abhilesh
    • B01D15/18C11B3/10C11C1/00C11C1/08
    • C11C1/02B01D15/185B01D15/1892C11B7/0008C11B7/0025C11C1/005C11C1/007
    • The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture, which process comprises the steps of: (i) purifying the feed mixture in a first separation step in a simulated or actual moving bed chromatography apparatus having a plurality of linked chromatography columns containing, as eluent, an aqueous organic solvent, to obtain an intermediate product; and (ii) purifying the intermediate product obtained in (i) in a second separation step using a simulated or actual moving bed chromatography apparatus having a plurality of linked chromatography columns containing, as eluent, an aqueous organic solvent, to obtain the PUFA product; wherein (a) the first and second separation steps are carried out sequentially on the same chromatography apparatus, the intermediate product being recovered between the first and second separation steps and the process conditions in the chromatography apparatus being adjusted between the first and second separation steps such that the PUFA product is separated from different components of the feed mixture in each separation step; or (b) the first and second separation steps are carried out on separate first and second chromatography apparatuses respectively, the intermediate product obtained from the first separation step being introduced into the second chromatography apparatus, and the PUFA product being separated from different components of the feed mixture in each separation step.
    • 本发明提供了用于从进料混合物中回收多不饱和脂肪酸(PUFA)产物的色谱分离方法,该方法包括以下步骤:(i)在模拟或实际移动床层析中在第一分离步骤中纯化进料混合物 具有多个连接的色谱柱的装置,其包含含水有机溶剂作为洗脱剂,以获得中间产物; 和(ii)使用具有多个相连的色谱柱的模拟或实际移动床层析装置,在第二分离步骤中纯化在(i)中获得的中间产物,所述色谱柱含有水性有机溶剂作为洗脱液,得到PUFA产物; 其中(a)第一和第二分离步骤在相同的色谱装置上依次进行,中间产物在第一和第二分离步骤之间回收,色谱装置中的工艺条件在第一和第二分离步骤之间进行调整, 在每个分离步骤中将PUFA产物与进料混合物的不同组分分离; 或(b)第一和第二分离步骤分别在分离的第一和第二色谱装置上进行,将从第一分离步骤获得的中间产物引入第二色谱装置中,并将PUFA产物从 进料混合物。