专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2017160788A3 HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES 审中-公开
公开(公告)号：WO2017160788A3
公开(公告)日：2017-09-21
申请号：PCT/US2017/022232
申请日：2017-03-14
申请人： RGENE, INC.
发明人： ZHENG, Yu , HONG, Manqing
IPC分类号： C12P19/30 , C12P19/34 , C12Q1/68
摘要： Provided herein are compositions, systems, and methods employing hyper-thermostable lysine-mutant ssDNA/RNA ligases that possesses both ssRNA ligase and ssDNA ligase activity. In certain embodiments, such hyper-thermostable lysine-mutant ssDNA/RNA ligases are used to ligate an first single stranded nucleic acid sequence with a 5' adenylated end to a second single stranded nucleic acid sequence (e.g., at a temperature of at least 75°C) to form a ligated nucleic acid sequence. In further embodiments, the ligated nucleic acid sequence is sequenced.

2. 发明申请

WO2017139260A1 MULTIPLE LIGASE COMPOSITIONS, SYSTEMS, AND METHODS 审中-公开
标题翻译：多重凝固酶组合物，系统和方法
公开(公告)号：WO2017139260A1
公开(公告)日：2017-08-17
申请号：PCT/US2017/016812
申请日：2017-02-07
申请人： RGENE, INC.
发明人： ZHENG, Yu , HONG, Manqing
IPC分类号： C12N9/00 , C12N15/10 , C07K14/01 , C07H21/04 , C12P19/34
CPC分类号： C12N9/93 , C12Y605/01001 , C12Y605/01002
摘要： Provided herein are compositions, systems, and methods using multiple ligases, wherein at least one of the ligases is an adenylation-deficient ATP-dependent ligase or an un-adenylated ATP-dependent ligase (e.g., present in an ATP free mixture). In certain embodiments, multiple ligases are used to ligate a pre-adenylated double stranded sequence to a non-adenylated double stranded sequence (e.g., the adenylation-deficient ATP-dependent ligase or un-adenylated ATP-dependent ligase ligates the first strand, and a second ligase ligates the second strand). In other embodiments, provided herein are mutant T4 ligases (e.g., K159S mutant or K159C mutant).
摘要翻译：本文提供了使用多连接酶的组合物，系统和方法，其中至少一个连接酶是腺苷酸化缺陷的ATP依赖性连接酶或非腺苷酸化的ATP依赖性连接酶（例如存在在无ATP的混合物中）。在某些实施方案中，多个连接酶用于将前腺苷酸化双链序列连接至非腺苷酸化双链序列（例如腺苷酸缺陷型ATP依赖性连接酶或非腺苷酸化ATP依赖型连接酶连接第一链，并且第二连接酶连接第二链）。在其他实施方案中，本文提供突变体T4连接酶（例如，K159S突变体或K159C突变体）。

3. 发明申请

WO2017160788A2 HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES 审中-公开
标题翻译：超热稳定赖氨酸突变体ssDNA / RNA LIGASES
公开(公告)号：WO2017160788A2
公开(公告)日：2017-09-21
申请号：PCT/US2017/022232
申请日：2017-03-14
申请人： RGENE, INC.
发明人： ZHENG, Yu , HONG, Manqing
IPC分类号： C12Q1/68 , C07H21/04
摘要： Provided herein are compositions, systems, and methods employing hyper-thermostable lysine-mutant ssDNA/RNA ligases that possesses both ssRNA ligase and ssDNA ligase activity. In certain embodiments, such hyper-thermostable lysine-mutant ssDNA/RNA ligases are used to ligate an first single stranded nucleic acid sequence with a 5' adenylated end to a second single stranded nucleic acid sequence (e.g., at a temperature of at least 75°C) to form a ligated nucleic acid sequence. In further embodiments, the ligated nucleic acid sequence is sequenced.
摘要翻译：本文提供了使用具有ssRNA连接酶和ssDNA连接酶活性的超耐热赖氨酸突变型ssDNA / RNA连接酶的组合物，系统和方法。在某些实施方案中，这样的高热稳定性赖氨酸突变体ssDNA / RNA连接酶用于将具有5'腺苷酸化末端的第一单链核酸序列与第二单链核酸序列连接（例如，在至少75℃ ; C）以形成连接的核酸序列。在进一步的实施方案中，对连接的核酸序列进行测序。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式