基本信息:
- 专利标题： HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES
- 申请号：PCT/US2017/022232 申请日：2017-03-14
- 公开(公告)号：WO2017160788A3 公开(公告)日：2017-09-21
- 发明人： ZHENG, Yu , HONG, Manqing
- 申请人： RGENE, INC.
- 申请人地址： 953 Indiana Street San Francisco, California 94107 US
- 专利权人： RGENE, INC.
- 当前专利权人： RGENE, INC.
- 当前专利权人地址： 953 Indiana Street San Francisco, California 94107 US
- 代理机构： BOND, Jason R.
- 优先权： US62/307,658 20160314
- 主分类号： C12P19/30
- IPC分类号： C12P19/30 ; C12P19/34 ; C12Q1/68
摘要：
Provided herein are compositions, systems, and methods employing hyper-thermostable lysine-mutant ssDNA/RNA ligases that possesses both ssRNA ligase and ssDNA ligase activity. In certain embodiments, such hyper-thermostable lysine-mutant ssDNA/RNA ligases are used to ligate an first single stranded nucleic acid sequence with a 5' adenylated end to a second single stranded nucleic acid sequence (e.g., at a temperature of at least 75°C) to form a ligated nucleic acid sequence. In further embodiments, the ligated nucleic acid sequence is sequenced.
公开/授权文献:
- WO2017160788A2 HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES 公开/授权日：2017-09-21
IPC结构图谱:
| C | 化学；冶金 |
| --C12 | 生物化学；啤酒；烈性酒；果汁酒；醋；微生物学；酶学；突变或遗传工程 |
| ----C12P | 发酵或使用酶的方法合成目标化合物或组合物或从外消旋混合物中分离旋光异构体 |
| ------C12P19/00 | 含有糖残基的化合物的制备 |
| --------C12P19/26 | .含氮碳水化合物的制备 |
| ----------C12P19/28 | ..N-葡萄糖苷 |
| ------------C12P19/30 | ...核苷酸 |