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    • 9. 发明申请
    • METABOLICALLY ENHANCED CYANOBACTERIUM WITH SEQUENTIALLY INDUCIBLE PRODUCTION GENES FOR THE PRODUCTION OF A FIRST CHEMICAL COMPOUND
    • 具有生产第一化学化合物的具有连续可诱导生产基因的代谢增强的细胞因子
    • WO2013098265A1
    • 2013-07-04
    • PCT/EP2012/076786
    • 2012-12-21
    • ALGENOL BIOFUELS INC.DÜHRING, Ulf
    • DÜHRING, UlfFRIEDRICH, AlexandraBAIER, KerstinENKE, HeikeKRAMER, Dan
    • C12N15/74C12P7/06
    • C12P7/065C12N15/52C12N15/74C12N15/8243Y02E50/17
    • This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds. Further provisions of the present invention include, inter alia, a method for producing the metabolically enhanced cyanobacterium and a method for producing a first chemical compound by culturing the metabolically enhanced cyanobacterium.
    • 本发明提供了一种代谢增强的蓝细菌,用于生产感兴趣的化合物,其具有至少两个编码第一生物催化剂的第一生产基因用于生产第一化合物。 两个第一个生产基因之一是在第一个生产基因的第一个启动子的转录控制下,而两个第一个生产基因中的另一个是第一个生产基因的第二个启动子的转录控制。 第一启动子和第二启动子在不同条件下分别诱导,并且至少两个第一生物催化剂催化相同的化学反应。 根据本发明的代谢增强的蓝细菌允许长期生产第一化合物。 本发明的进一步规定尤其包括生产代谢增强的蓝细菌的方法和通过培养代谢增强的蓝细菌来生产第一化合物的方法。