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1. 发明申请

WO0058503A3 IMPROVED METHOD FOR DETECTING MICRO-ORGANISMS 审中-公开
标题翻译：用于检测微量有机物的改进方法
公开(公告)号：WO0058503A3
公开(公告)日：2001-02-01
申请号：PCT/GB0001224
申请日：2000-03-30
申请人： ZETATRONICS LTD , GRANT KATHLEEN ANN , HARBRON STUART , WILLIAMS DAVID ROSS , YOUNG GORDON CHALMERS MELLIS
发明人： GRANT KATHLEEN ANN , HARBRON STUART , WILLIAMS DAVID ROSS , YOUNG GORDON CHALMERS MELLIS
IPC分类号： C12Q1/04 , C12Q1/18 , G01N27/447 , G01N33/487
CPC分类号： C12Q1/18 , C12Q1/04 , C12Q2304/80
摘要： A method of identifying one or more micro-organisms in a fluid comprising the steps of: (i) taking a sample of the fluid containing a representative sample of any micro-organism(s) present; (ii) optionally culturing the sample if necessary to increase the number of micro-organisms to a pre-determined range; (iii) suspending aliquots of the fluid containing the micro-organism(s) in a plurality of different solutions, said solutions representing different experimental conditions differing in one or more of the parameters selected from the group of parameters consisting of pH, ionic strength, concentration of surface-active agent, concentration of heavy metal ions, concentration of chelating or complexing agent, concentration of organic solvent, concentration of dye or stain, concentration of polyol, or concentration of polyether; (iv) applying an electric field across a portion of said fluid; (v) measuring the velocity, displacement, zeta potential or electrophoretic mobility of any micro-organisms present following the application of said electric field; (vi) comparing said measured velocities, displacements, zeta potentials or electrophoretic mobilities with tables of velocities, displacements, zeta potentials or electrophoretic mobilities of known micro-organisms measured under substantially identical experimental conditions to determine which, if any, micro-organisms are present.
摘要翻译：一种识别流体中的一种或多种微生物的方法，包括以下步骤：（i）取含含有存在的任何微生物的代表性样品的流体样品; （ii）如果需要可选地培养样品以将微生物数量增加到预定范围; （iii）将含有微生物的流体的等分试样悬浮在多种不同的溶液中，所述溶液代表不同的实验条件，其中一个或多个参数选自由pH，离子强度，表面活性剂浓度，重金属离子浓度，螯合剂或络合剂浓度，有机溶剂浓度，染料或染色浓度，多元醇浓度或聚醚浓度; （iv）在所述流体的一部分上施加电场; （v）测量施加所述电场后存在的任何微生物的速度，位移，ζ电位或电泳迁移率; （vi）将所测量的速度，位移，ζ电位或电泳迁移率与基本上相同的实验条件下测量的已知微生物的速度，位移，ζ电位或电泳迁移率进行比较，以确定存在微生物（如果有的话）。

2. 发明申请

WO02065124A3 IMPROVED METHOD FOR DETECTING CELLS 审中-公开
标题翻译：改进的检测细胞的方法
公开(公告)号：WO02065124A3
公开(公告)日：2003-10-16
申请号：PCT/GB0200642
申请日：2002-02-14
申请人： ZETATRONICS LTD , HARBRON STUART , WHITEHOUSE DAVID BERTRAM , WILLIAMS DAVID ROSS , GRANT KATHLEEN ANN
发明人： HARBRON STUART , WHITEHOUSE DAVID BERTRAM , WILLIAMS DAVID ROSS , GRANT KATHLEEN ANN
IPC分类号： G01N33/569 , G01N33/53 , G01N33/58
CPC分类号： G01N33/56905
摘要： A method for detecting the presence of cells or other target biological entities in a fluid sample, which method comprises: a) contacting the sample with a specific binding partner having (i) an electrophoretic, or zeta potential, label or (ii) a fluorescence label; b) allowing the specific binding partner to bind to any said cell or other target biological entity present in the fluid sample; c) in an electric field measuring the value of the velocity, displacement, zeta potential or electrophoretic mobility of any said cell or other target biological entity present in the fluid sample and that is bound to the specific binding partner; and d) observing the value obtained in step c) as indicative of the presence of a said cell or other target biological entity in the fluid samples.
摘要翻译：一种用于检测流体样品中细胞或其他靶生物实体的存在的方法，所述方法包括：a）使样品与具有（i）电泳或ζ电位标记的特异性结合配偶体接触，或（ii）荧光标签; b）允许特异性结合配偶体结合存在于流体样品中的任何所述细胞或其它目标生物实体; c）在电场中测量流体样品中存在并且结合于特异性结合配偶体的任何所述细胞或其它目标生物实体的速度，位移，ζ电位或电泳迁移率的值; 以及d）观察在步骤c）中获得的值作为流体样品中存在所述细胞或其它目标生物实体的指示。

3. 发明申请

WO0161029A3 IDENTIFICATION METHOD 审中-公开
标题翻译：识别方法
公开(公告)号：WO0161029A3
公开(公告)日：2002-04-04
申请号：PCT/GB0100658
申请日：2001-02-16
申请人： ZETATRONICS LTD , GRANT KATHLEEN ANN , HARBRON STUART , WILLIAMS DAVID ROSS
发明人： GRANT KATHLEEN ANN , HARBRON STUART , WILLIAMS DAVID ROSS
IPC分类号： C12Q1/04 , G01N27/447 , C12Q1/34
CPC分类号： G01N27/447 , C12Q1/04 , C12Q2304/80
摘要： A method of identifying one or more micro-organisms in a fluid sample, said method comprising the steps of: (i) optionally culturing the sample if necessary to increase the number of micro-organisms to a pre-determined range; (ii) applying an electric field across a portion of said fluid; (iii) measuring the velocity, displacement, zeta potential or electrophoretic mobility of any micro-organisms present following the application of said electric field; (iv) re-measuring the velocity, displacement, zeta potential or electrophoretic mobility of the fluid sample after incubation in the presence of a bioactive peptide; (v) comparing said measured velocities, displacements, zeta potentials or electrophoretic mobilities with tables of velocities, displacements, zeta potentials or electrophoretic mobilities of known micro-organisms measured under substantially identical experimental conditions to determine which, if any, micro-organisms are present.
摘要翻译：一种识别流体样品中的一种或多种微生物的方法，所述方法包括以下步骤：（i）如果需要，任选地培养样品以将微生物数量增加到预定范围; （ii）在所述流体的一部分上施加电场; （iii）测量施加所述电场后存在的任何微生物的速度，位移，ζ电位或电泳迁移率; （iv）在生物活性肽存在下重新测量流体样品在温育后的速度，位移，ζ电位或电泳迁移率; （v）将所测量的速度，位移，ζ电位或电泳迁移率与基本上相同的实验条件下测量的已知微生物的速度，位移，ζ电位或电泳迁移率进行比较，以确定存在微生物（如果有的话）。

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