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1. 发明申请

WO0192870A3 KIT AND METHOD FOR DETERMINING A PLURALITY OF ANALYTES 审中-公开
标题翻译： KIT AND METHOD FOR MULTIANALYTBESTIMMUNG
公开(公告)号：WO0192870A3
公开(公告)日：2002-10-03
申请号：PCT/EP0105995
申请日：2001-05-25
申请人： ZEPTOSENS AG , PAWLAK MICHAEL , SCHICK EGINHARD , ABEL ANDREAS P , DUVENECK GERT L , EHRAT MARKUS , KRESBACH GERHARD M , SCHUERMANN-MADER EVELINE , BOPP MARTIN A
发明人： PAWLAK MICHAEL , SCHICK EGINHARD , ABEL ANDREAS P , DUVENECK GERT L , EHRAT MARKUS , KRESBACH GERHARD M , SCHUERMANN-MADER EVELINE , BOPP MARTIN A
IPC分类号： B01L3/00 , G01N21/25 , G01N21/64 , G01N21/76 , G01N33/15 , G01N33/53 , G01N33/543 , G01N33/569 , G01N37/00
CPC分类号： G01N21/6452 , B01L3/5085 , G01N21/648 , G01N33/54373 , G01N2021/6441 , G01N2021/6482 , Y10S435/808 , Y10S435/975 , Y10S436/805 , Y10S436/808
摘要： The invention relates to various embodiments of a kit for simultaneous, qualitative and/or quantitative detection of a plurality of analytes, comprising a sensor platform consisting of a thin-layered optical wave guide with a layer (a) which is transparent at least at one excitation wavelength on a layer (b) which is also transparent at least at the same excitation wavelength, having a lower refraction index than layer (a), and at least one lattice structure (c) modulated in layer (a); in addition to at least one array of biochemical or synthetic detector elements immobilized on layer (a) and disposed in discrete measuring areas (d) either directly or by means of an adhesion promoting layer. Said detector elements are used for specific detection and/or bonding of said anayltes and/or specific interaction therewith. The inventive kit also contains means for local resolution referencing of the excitation light intensity present in the measuring areas in addition to, optionally, means for calibrating at least one luminescence produced as a result of bonding between one or several analytes or as a result of the specific interaction with one or several analytes in the near field of layer (a). A liquid sample in which the analytes are to be examined is brought into contact, either directly or after mixing it with other reagents, with the measuring areas on the sensor platform. The invention also relates to analytic systems based on the inventive kit, methods which are conducted in order to detect one or several analytes and the use thereof.
摘要翻译：本发明涉及用于多种分析物的同时定性和/或定量检测的试剂盒的各种实施例，包括：一个传感器平台至少在上一个同样透明的激发波长层（a）在至少这个激发波长层（b包括具有透明的光学薄膜波导）具有比层低的折射率调制（a）和在该层的至少一个：（a）光栅结构（c）中，用于耦合所述激发光在层（a）; 在直接或通过（a）中固定化的生物或生物化学的或合成的识别元件的特异性识别和/或结合层上的粘合剂层离散测量区（d）至少一个阵列，所述分析物和/或与所述分析物的特异性相互作用; 注意事项的空间分辨的参考光强度和任选的范围的可用激励; 由于结合的一种或多种分析物或作为与在层的近场中的一个或多个分析物的特异性相互作用的结果，安排所产生的一个或多种发光的校准（a）中，向所述被检液体样品的分析物直接地或与其它试剂混合后与所述上测量区，所述传感器平台接触。本发明还涉及新颖的基于试剂盒的分析系统，以及执行的方法，用于检测一种或多种分析物和它们的用途。

2. 发明申请

WO2004023143A3 ANALYTICAL PLATFORM AND IDENTIFICATION METHOD WITH ANALYTES, WHICH ARE TO BE IDENTIFIED IN A SAMPLE OPTIONALLY AFTER FRACTIONATION AND WHICH SERVE AS IMMOBILIZED SPECIFIC BINDING PARTNERS 审中-公开
标题翻译：分析平台及检测方法
公开(公告)号：WO2004023143A3
公开(公告)日：2004-04-29
申请号：PCT/EP0309562
申请日：2003-08-28
申请人： ZEPTOSENS AG , PAWLAK MICHAEL , SCHICK EGINHARD , OROSZLAN PETER
发明人： PAWLAK MICHAEL , SCHICK EGINHARD , OROSZLAN PETER
IPC分类号： G01N33/53 , C12M1/00 , C12M1/34 , C12Q1/02 , C12Q1/68 , G01N21/25 , G01N21/55 , G01N21/64 , G01N21/76 , G01N21/78 , G01N27/447 , G01N30/88 , G01N33/543 , G01N33/553 , G01N33/577 , G01N21/77
CPC分类号： G01N21/6452 , G01N21/553 , G01N21/648 , G01N33/54373 , G01N2021/6441
摘要： The invention relates to an analytical platform and to a method carried out therewith for examining a multitude of samples for the presence of compounds, which are denoted as analytes, contained in the samples, and are biologically relevant as participants in specific binding reactions. The invention is characterized in that said samples or fractions of these samples along with the analytes, which are to be identified and contained therein and which serve as a first multitude of specific binding partners, are placed in discrete measuring areas in at least one one-dimensional or two-dimensional array on an evanescent field sensor platform, which serves as a solid support, directly or after additional dilutions of the samples or of the fractions. Different samples or fractions or different dilutions of samples or fractions are arranged in different discrete measuring areas. One or more identifying substances, which serve as a second multitude of specific binding partners and which are provided for specifically identifying one or more analytes contained in the samples that are from said first multitude of specific binding partners, are, in a single or a number of steps of a specific binding reaction, brought into contact with the samples or their fractions or their dilutions placed in said discrete measuring areas. Changes in optoelectronic signals due to the binding of identifying substances to analytes contained in discrete measuring areas are measured in a locally resolved manner in the evanescent field of the evanescent field sensor platform, and the presence of the analytes to be specifically identified in the respective measuring areas is determined qualitatively and/or quantitatively on the basis of the relative magnitude of the changes in said optoelectronic signals from the respective measuring areas.
摘要翻译：本发明涉及的分析平台和用于分析多个样品到包含在样品，如在特定的结合反应的生物相关化合物作为分析物的参与者，其特征在于，如此实现的方法：所述与在其中样品含有的样品或分数待检测的分析物，如第一多个特异性结合伴侣的，一个直接或之后在至少一个或在离散测量区zweidimensisonalen阵列的渐逝场传感器平台上的样品或组分的附加的稀释液可以用作固体支持物，其特征在于，不同的样品或级分或不同稀释度的被设置的各种样品或馏分离散测量区中; 一种或多种检测试剂，作为第二多个特异性结合配偶的用于包含在从所述第一组多个特异性结合伴侣的，与所述沉积到的特异结合反应的一个或多个步骤的样品被分析物的一种或多种特异性检测所述离散测量区的样品或其馏分或其稀释液进行接触; 从变化的相对大小测量并定性地说，从各自的测量区域和/或光电信号作为分析物包含在离散测量区的结合的结果的量的变化的光电信号在渐逝场传感器平台以空间的渐逝场解析分析物的存在下待确定存在特定分析物。

3. 发明申请

WO2004023143A8 ANALYTICAL PLATFORM AND IDENTIFICATION METHOD 审中-公开
标题翻译：分析平台和证明方法
公开(公告)号：WO2004023143A8
公开(公告)日：2004-07-22
申请号：PCT/EP0309562
申请日：2003-08-28
申请人： ZEPTOSENS AG , PAWLAK MICHAEL , SCHICK EGINHARD , OROSZLAN PETER
发明人： PAWLAK MICHAEL , SCHICK EGINHARD , OROSZLAN PETER
IPC分类号： G01N33/53 , C12M1/00 , C12M1/34 , C12Q1/02 , C12Q1/68 , G01N21/25 , G01N21/55 , G01N21/64 , G01N21/76 , G01N21/78 , G01N27/447 , G01N30/88 , G01N33/543 , G01N33/553 , G01N33/577 , G01N21/77
CPC分类号： G01N21/6452 , G01N21/553 , G01N21/648 , G01N33/54373 , G01N2021/6441
摘要： The invention relates to an analytical platform and to a method carried out therewith for examining a multitude of samples for the presence of compounds, which are denoted as analytes, contained in the samples, and are biologically relevant as participants in specific binding reactions. The invention is characterized in that said samples or fractions of these samples along with the analytes, which are to be identified and contained therein and which serve as a first multitude of specific binding partners, are placed in discrete measuring areas in at least one one-dimensional or two-dimensional array on an evanescent field sensor platform, which serves as a solid support, directly or after additional dilutions of the samples or of the fractions. Different samples or fractions or different dilutions of samples or fractions are arranged in different discrete measuring areas. One or more identifying substances, which serve as a second multitude of specific binding partners and which are provided for specifically identifying one or more analytes contained in the samples that are from said first multitude of specific binding partners, are, in a single or a number of steps of a specific binding reaction, brought into contact with the samples or their fractions or their dilutions placed in said discrete measuring areas. Changes in optoelectronic signals due to the binding of identifying substances to analytes contained in discrete measuring areas are measured in a locally resolved manner in the evanescent field of the evanescent field sensor platform, and the presence of the analytes to be specifically identified in the respective measuring areas is determined qualitatively and/or quantitatively on the basis of the relative magnitude of the changes in said optoelectronic signals from the respective measuring areas.
摘要翻译：本发明涉及的分析平台和用于分析多个样品到包含在样品，如在特定的结合反应的生物相关化合物作为分析物的参与者，其特征在于，如此实现的方法：所述与在其中样品含有的样品或分数待检测的分析物，如第一多个特异性结合伴侣的，一个直接或之后在至少一个或在离散测量区zweidimensisonalen阵列的渐逝场传感器平台上的样品或组分的附加的稀释液可以用作固体支持物，其特征在于，不同的样品或级分或不同稀释度的排列不同离散测量范围内的样品或分数; 一种或多种检测试剂，作为第二多个特异性结合配偶的用于包含在从所述第一组多个特异性结合伴侣的，与所述沉积到的特异结合反应的一个或多个步骤的样品被分析物的一种或多种特异性检测所述离散测量区的样品或他们的分数或他们的稀释物接触; 从变化的相对大小测量并定性地说，从各自的测量区域和/或光电信号作为分析物包含在离散测量区的结合的结果的量的变化的光电信号在渐逝场传感器平台以空间的渐逝场解析分析物的存在下有待检测的特定分析物。

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