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1. 发明申请

WO0153306A3 MANUFACTURE OF FIVE-CARBON SUGARS AND SUGAR ALCOHOLS 审中-公开
标题翻译：五碳糖和糖酒的制造
公开(公告)号：WO0153306A3
公开(公告)日：2002-04-18
申请号：PCT/FI0100051
申请日：2001-01-22
申请人： XYROFIN OY , MIASNIKOV ANDREI , OJAMO HEIKKI , POVELAINEN MIRA , GROS HAAKAN , TOIVARI MERVI , RICHARD PETER , RUOHONEN LAURA , KOIVURANTA KARI , LONDESBOROUGH JOHN , ARISTIDOU ARISTOS , PENTTILAE MERJA , PLAZANET MENUT CLAIRE , DEUTSCHER JOSEF
发明人： MIASNIKOV ANDREI , OJAMO HEIKKI , POVELAINEN MIRA , GROS HAAKAN , TOIVARI MERVI , RICHARD PETER , RUOHONEN LAURA , KOIVURANTA KARI , LONDESBOROUGH JOHN , ARISTIDOU ARISTOS , PENTTILAE MERJA , PLAZANET-MENUT CLAIRE , DEUTSCHER JOSEF
IPC分类号： C12N15/09 , C07H1/00 , C07H3/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/04 , C12P7/18 , C12N15/52
CPC分类号： C07H1/00 , C07H3/02
摘要： The invention relates to the methods of manufacturing five-carbon sugars and sugar alcohols as well as other compounds derived from pentose-phosphate pathway from readily available substrates such a hexoses using metabolically engineered microbial hosts.
摘要翻译：本发明涉及制备五碳糖和糖醇的方法以及衍生自可利用底物的戊糖磷酸途径的其它化合物，例如使用代谢工程的微生物宿主的己糖。

2. 发明申请

WO2006008653A9 LIPOLYTIC ENZYME USES THEREOF IN THE FOOD INDUSTRY 审中-公开
标题翻译：脂肪酶在食品工业中的应用
公开(公告)号：WO2006008653A9
公开(公告)日：2007-02-22
申请号：PCT/IB2005002602
申请日：2005-07-18
申请人： DANISCO , MIASNIKOV ANDREI , SOEE JOERN BORCH , MIKKELSEN JOERN DALGAARD , POVELAINEN MIRA , PITKANEN VIRVE
发明人： MIASNIKOV ANDREI , SOEE JOERN BORCH , MIKKELSEN JOERN DALGAARD , POVELAINEN MIRA , PITKANEN VIRVE
IPC分类号： C11B3/00 , A21D2/26 , A23C9/13 , A23L9/10 , A23L13/00 , A23L13/40 , A23L13/50 , A23L15/00 , A23L17/00 , A23L27/24 , A23L27/60 , A23L29/00 , C07F9/10 , C11C3/00 , C12N9/20 , C12N15/55 , C12P7/64
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention also encompases the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.
摘要翻译：本发明还包括在各种方法和用途中可使用从下列属之一获得的脂解酶：链霉菌属，棒状杆菌属和嗜热菌属，其中所述脂解酶能够水解糖脂或磷脂或从糖脂转移酰基或磷脂酰基受体。优选地，用于这些方法和用途的脂解酶包含如SEQ.ID.NO.1中任一所示的氨基酸序列。 ID。编号为4,5,7,8,12,14或16的氨基酸序列或与其具有至少70％同一性的氨基酸序列，或者包含如SEQ ID No.3,6,9,13,15或17所示的核苷酸序列或与其具有至少70％同一性的核苷酸序列。本发明还涉及能够水解至少半乳糖脂或能够将酰基从半乳糖脂转移到一个或多个酰基受体底物的脂肪分解酶，其中所述酶可以从链霉菌种获得并且包括含有氨基酸的脂解酶如SEQ ID No.4所示的氨基酸序列或与其具有至少60％同一性的氨基酸序列。该酶可以由选自以下的核酸编码：a）包含SEQ ID No.3所示的核苷酸序列的核酸; b）通过遗传密码的变性与核苷酸序列SEQ ID No.3相关的核酸; 和c）包含与SEQ ID No.3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

3. 发明申请

WO2006008653A3 LIPOLYTIC ENZYME USES THEREOF IN THE FOOD INDUSTRY 审中-公开
标题翻译：脂肪酶在食品工业中的应用
公开(公告)号：WO2006008653A3
公开(公告)日：2007-03-29
申请号：PCT/IB2005002602
申请日：2005-07-18
申请人： DANISCO , MIASNIKOV ANDREI , SOEE JOERN BORCH , MIKKELSEN JOERN DALGAARD , POVELAINEN MIRA , PITKANEN VIRVE
发明人： MIASNIKOV ANDREI , SOEE JOERN BORCH , MIKKELSEN JOERN DALGAARD , POVELAINEN MIRA , PITKANEN VIRVE
IPC分类号： C11B3/00 , A21D2/26 , A23C9/13 , A23L9/10 , A23L13/00 , A23L13/40 , A23L13/50 , A23L15/00 , A23L17/00 , A23L27/24 , A23L27/60 , A23L29/00 , C07F9/10 , C11C3/00 , C12N9/20 , C12N15/55 , C12P7/64
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention also encompases the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.
摘要翻译：本发明还包括使用可从各种方法获得的脂肪分解酶：链霉菌，棒状杆菌和热嗜热菌，其中所述脂肪分解酶能够水解糖脂或磷脂或从糖脂转移酰基或磷脂转化为酰基受体。优选地，用于这些方法和用途的脂肪分解酶包含SEQ ID NO：1中任一项所示的氨基酸序列。 ID。第4,5,7,8,12,14或16号氨基酸序列或与其具有至少70％同一性的氨基酸序列，或包含SEQ ID No.3,6,13,13,15或17所示的核苷酸序列或与其具有至少70％同一性的nucceotide seqonunce。本发明还涉及能够至少水解半乳糖脂或能够将酰基从半乳糖脂转移至一种或多种酰基受体底物的脂解酶，其中所述酶可从步态物种获得，并且包括脂肪分解酶，其包含氨基如SEQ ID No.4所示的酸序列或与其具有至少60％同一性的氨基酸序列。该酶可以由选自以下的核酸编码：a）包含SEQ ID No.3所示核苷酸序列的核酸; b）通过遗传密码的退化与SEQ ID No.3的核苷酸序列相关的核酸; 和c）包含与SEQ ID No.3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

4. 发明申请

WO2009132220A3 ISOPRENE SYNTHASE VARIANTS FOR IMPROVED MICROBIAL PRODUCTION OF ISOPRENE 审中-公开
标题翻译：异丙烯合成酶变异体用于改进微生物生产异戊二烯
公开(公告)号：WO2009132220A3
公开(公告)日：2010-01-21
申请号：PCT/US2009041581
申请日：2009-04-23
申请人： DANISCO US INC , GOODYEAR TIRE & RUBBER , CERVIN MARGUERITE A , WHITED GREGORY M , MIASNIKOV ANDREI , PERES CAROLINE M , WEYLER WALTER , WELLS DEREK H , BOTT RICHARD R
发明人： CERVIN MARGUERITE A , WHITED GREGORY M , MIASNIKOV ANDREI , PERES CAROLINE M , WEYLER WALTER , WELLS DEREK H , BOTT RICHARD R
IPC分类号： C12N9/88 , C12N5/10 , C12N15/52
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译：本发明提供了包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。具体而言，本发明提供了用于增加微生物宿主细胞中异戊二烯产生的变异植物异戊二烯合酶。本发明的生物合成产生的异戊二烯可用于制造橡胶和弹性体。

5. 发明申请

WO2008097619A3 VARIANT BUTTIAUXELLA SP. PHYTASES HAVING ALTERED PROPERTIES 审中-公开
标题翻译： VARIANT BUTTIAUXELLA SP。具有改性特性的植物
公开(公告)号：WO2008097619A3
公开(公告)日：2008-10-16
申请号：PCT/US2008001646
申请日：2008-02-06
申请人： DANISCO US INC GENENCOR DIV , CERVIN MARGUERITE A , KENSCH OLIVER , KETTLING ULRICH , KIM STEVE , LEUTHNER BIRGITTA , MIASNIKOV ANDREI , PELLENGAHR KLAUS , WARD MICHAEL
发明人： CERVIN MARGUERITE A , KENSCH OLIVER , KETTLING ULRICH , KIM STEVE , LEUTHNER BIRGITTA , MIASNIKOV ANDREI , PELLENGAHR KLAUS , WARD MICHAEL
IPC分类号： C12N15/55 , C12N9/16
CPC分类号： C12N9/16 , A23K20/189 , A23K50/10 , A23K50/30 , A23K50/75 , A23K50/80 , C12Y301/03008 , C12Y301/03026 , C13K1/06 , Y02A40/818 , Y02E50/17
摘要： The present invention relates to variant phytase enzymes having altered properties
摘要翻译：本发明涉及具有改变性质的变体植酸酶

6. 发明申请

WO2012054554A3 THERMOSTABLE TRICHODERMA CELLULASE 审中-公开
标题翻译：耐热TRICHODERMA纤维素酶
公开(公告)号：WO2012054554A3
公开(公告)日：2013-10-24
申请号：PCT/US2011056810
申请日：2011-10-19
申请人： DANISCO US INC , MIASNIKOV ANDREI , SCHELLE MICHAEL , WARD MICHAEL
发明人： MIASNIKOV ANDREI , SCHELLE MICHAEL , WARD MICHAEL
IPC分类号： C12P21/00 , C12N1/15
CPC分类号： C12N9/2437 , C07K14/37 , C12N15/80 , C12P21/02 , C12Y302/01004
摘要： Described are compositions and methods relating to the thermostable fungal cellulase enzyme, EGV, and Trichoderma host cells having a modification comprising or consisting essentially of disruption or deletion of nucleotide(s) for expression of this cellulose, whereby EGV expression is prevented.
摘要翻译：描述了与热稳定性真菌纤维素酶，EGV和木霉属宿主细胞相关的组合物和方法，其具有包含或基本上由此表达该纤维素的核苷酸的或由其组成的修饰，由此可以预防EGV表达。

7. 发明申请

WO2009132220A9 ISOPRENE SYNTHASE VARIANTS FOR IMPROVED MICROBIAL PRODUCTION OF ISOPRENE 审中-公开
标题翻译：用于改善微生物生产异烟肼的异丙肾上腺素变异体
公开(公告)号：WO2009132220A9
公开(公告)日：2010-05-06
申请号：PCT/US2009041581
申请日：2009-04-23
申请人： DANISCO US INC , GOODYEAR TIRE & RUBBER , CERVIN MARGUERITE A , WHITED GREGORY M , MIASNIKOV ANDREI , PERES CAROLINE M , WEYLER WALTER , WELLS DEREK H , BOTT RICHARD R , RIFE CHRISTOPHER LEE
发明人： CERVIN MARGUERITE A , WHITED GREGORY M , MIASNIKOV ANDREI , PERES CAROLINE M , WEYLER WALTER , WELLS DEREK H , BOTT RICHARD R , RIFE CHRISTOPHER LEE
IPC分类号： C12N9/88 , C12N5/10 , C12N15/52
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译：本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。

8. 发明申请

WO2009100183A2 VARIANT BUTTIAUXELA SP. PHYTASES HAVING ALTERED PROPERTIES 审中-公开
标题翻译： VARIANT BUTTIAUXELA SP。具有改性特性的植物
公开(公告)号：WO2009100183A2
公开(公告)日：2009-08-13
申请号：PCT/US2009033162
申请日：2009-02-05
申请人： DANISCO US INC GENENCOR DIV , CERVIN MARGUERITE A , KENSCH OLIVER , KETTLING ULRICH , KIM STEVEN S , LEUTHNER BIRGITTA , MIASNIKOV ANDREI , PELLENGAHR KLAUS , WARD MICHAEL
发明人： CERVIN MARGUERITE A , KENSCH OLIVER , KETTLING ULRICH , KIM STEVEN S , LEUTHNER BIRGITTA , MIASNIKOV ANDREI , PELLENGAHR KLAUS , WARD MICHAEL
IPC分类号： C12N9/16
CPC分类号： C12N9/16 , C12N9/242 , C12P7/06 , C12P19/14 , Y02E50/17
摘要： The present invention relates to variant phytase enzymes having altered properties.
摘要翻译：本发明涉及具有改变性质的变体植酸酶。

9. 发明申请

WO2010107560A3 FUNGAL CUTINASE FROM MAGNAPORTHE GRISEA 审中-公开
标题翻译：来自MAGNAPORTHE GRISEA的FUNGAL CUTINASE
公开(公告)号：WO2010107560A3
公开(公告)日：2010-12-16
申请号：PCT/US2010025254
申请日：2010-02-24
申请人： DANISCO US INC , ADAMS CHRISTIAN D , MIASNIKOV ANDREI
发明人： ADAMS CHRISTIAN D , MIASNIKOV ANDREI
IPC分类号： C07K14/37 , C11D3/00 , C12N9/10 , C12N9/14
CPC分类号： C12N9/18 , C11D3/38627 , C11D3/38681 , C12P7/6454 , C12Y301/01074
摘要： Described are compositions and methods relating to a fungal cutinase cloned from Magnaporthe grisea, polynucleotides encoding the cutinase, and methods of use thereof. The compositions and methods have particular application in detergent cleaning compositions and synthesis reactions.
摘要翻译：描述了与从Magnaporthe grisea克隆的真菌角质酶相关的组合物和方法，以及编码角质酶的多核苷酸及其使用方法。组合物和方法在洗涤剂清洁组合物和合成反应中具有特别的应用。

10. 发明申请

WO2010065455A3 ENZYMES WITH LIPASE ACTIVITY 审中-公开
标题翻译：具有脂肪酶活性的酶
公开(公告)号：WO2010065455A3
公开(公告)日：2010-07-29
申请号：PCT/US2009066106
申请日：2009-11-30
申请人： DANISCO US INC , ADAMS CHRISTIAN D , BAO KAI , COLLIER KATHERINE D , LEE EDWIN , MIASNIKOV ANDREI , QIAN ZHEN , SCHMIDT BRIAN F , SONG DANFENG
发明人： ADAMS CHRISTIAN D , BAO KAI , COLLIER KATHERINE D , LEE EDWIN , MIASNIKOV ANDREI , QIAN ZHEN , SCHMIDT BRIAN F , SONG DANFENG
IPC分类号： C11D3/386
CPC分类号： C11D3/38627
摘要： Described are detergent compositions comprising at least one lipase enzyme selected from SriII, ScoIIA, ScoIIB, CefII, and variants, thereof. The compositions are useful for removing oily stains from fabric.
摘要翻译：描述了包含至少一种选自SriII，ScoIIA，ScoIIB，CefII及其变体的脂肪酶的洗涤剂组合物。该组合物可用于从织物中除去油性污渍。

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