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2. 发明申请

WO2011011660A2 DRAIN DOWN AND RE-FEED OF MICROCARRIER BIOREACTOR 审中-公开
标题翻译：微生物生物反应器的排水和再输送
公开(公告)号：WO2011011660A2
公开(公告)日：2011-01-27
申请号：PCT/US2010043013
申请日：2010-07-23
申请人： XCELLEREX INC , GUERTIN PATRICK M , CROWELL JOSEPH D
发明人： GUERTIN PATRICK M , CROWELL JOSEPH D
IPC分类号： C12N7/01
CPC分类号： C12N7/00 , C12M25/14 , C12M47/10 , C12N2770/24151
摘要： The present invention provides a method of increasing product yield per culture in a population of product-secreting cells bound to a scaffold, for example, a microcarrier, at least partially immersed in a culture medium in a bioreactor, the method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration from the bioreactor while leaving the scaffold with the bound population of product-secreting cells in the bioreactor; re-feeding the bound population of product-secreting cells by adding to the bioreactor an amount of a fresh culture medium sufficient to increase the volume of the culture medium in the bioreactor to approximately the original volume of the culture medium; agitating the culture medium under sufficient conditions and for a sufficient time period to allow the bound population of product-secreting cells to grow and to release a second-secreted product concentration into the culture medium; and harvesting product by removing the culture medium with the second- secreted product concentration from the bioreactor while leaving the scaffold with the bound population of product-secreting cells in the bioreactor. Also disclosed is a method of increasing virus yield per culture in a population of virus-infected cells bound to a microcarrier suspended in a culture medium in a bioreactor.
摘要翻译：本发明提供了一种在与生物反应器中至少部分浸入培养基中的支架例如微载体结合的产物分泌细胞群体中提高每种培养物产物产量的方法，所述方法包括：半收获通过从生物反应器中以一分泌产物浓度去除一定体积的培养基，同时在生物反应器中离开具有结合产物分泌细胞群的支架; 通过向生物反应器中加入一定量的足以将生物反应器中的培养基的体积增加到大约原始体积的培养基的新鲜培养基来再喂养结合的产物分泌细胞群; 在足够的条件下搅拌培养基足够的时间以允许结合的产物分泌细胞群体生长并将第二分泌的产物浓度释放到培养基中; 并通过从生物反应器中除去具有第二分泌产物浓度的培养基并在生物反应器中留下具有产物分泌细胞的结合群体的同时离开支架来收获产物。还公开了一种在生物反应器中悬浮于培养基中的微载体结合的病毒感染细胞群体中增加病毒产量的方法。

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