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1. 发明申请

WO2013032828A2 METHOD FOR THE PREPARATION OF A HEAT STABLE OXYGEN CARRIER-CONTAINING COMPOSITION FACILATING BETA-BETA CROSS-LINKING 审中-公开
标题翻译：制备提供β-β交联的热稳定载体组合物的方法
公开(公告)号：WO2013032828A2
公开(公告)日：2013-03-07
申请号：PCT/US2012/051959
申请日：2012-08-23
申请人： WONG, Bing, Lou , KWOK, Sui, Yi , BUTT, Kowk, Chu
发明人： WONG, Bing, Lou , KWOK, Sui, Yi , BUTT, Kowk, Chu
IPC分类号： A61K38/42 , A61K35/14 , A61K35/12 , A61K9/08 , A61K47/48
CPC分类号： A61K38/42
摘要： Methods for preparation of a heat stable hemoglobin based oxygen-carrier-containing pharmaceutical composition such that beta-beta cross-linking is favored are provided. Using the methods of the present invention, the oxygen affinity of the resultant molecule can be controlled so that hemoglobin based oxygen carriers tailored for specific applications can be produced. Lower oxygen affinity crosslinked hemoglobin is useful for applications requiring rapid tissue oxygenation (e.g. hemorrhagic shock) while higher oxygen affinity cross-linked hemoglobin is useful for applications requiring a slower rate of oxygenation (e.g. cancer adjunct therapy). A highly purified and heat stable crosslinked non-polymeric tetrameric hemoglobin having beta- beta cross-linking of greater than 40-60% and suitable for use in mammals without causing renal injury and vasoconstriction is produced. A high temperature and short time (HTST) heat processing step is performed to effectively remove any undesired dimeric hemoglobin, non- crosslinked tetrameric hemoglobin, and plasma protein impurities.
摘要翻译：提供了制备热稳定血红蛋白基含氧载体药物组合物以使β-β交联更受欢迎的方法。使用本发明的方法，可以控制所得分子的氧亲和力，从而可以生产针对具体应用定制的血红蛋白基氧载体。较低氧亲和力交联血红蛋白可用于需要快速组织氧合（例如失血性休克）的应用，而较高氧亲和力交联血红蛋白可用于需要较慢氧合速率的应用（例如癌症辅助治疗）。产生具有大于40-60％的β-β交联并且适用于哺乳动物而不引起肾损伤和血管收缩的高度纯化和热稳定的交联非聚合物四聚体血红蛋白。进行高温短时间（HTST）热处理步骤以有效除去任何不需要的二聚血红蛋白，非交联四聚体血红蛋白和血浆蛋白杂质。

2. 发明申请

WO2011162863A1 A METHOD FOR THE PREPARATION OF A HEAT STABLE OXYGEN CARRIER-CONTAINING PHARMACEUTICAL COMPOSITION 审中-公开
标题翻译：一种制备含稳定含氧载体的药物组合物的方法
公开(公告)号：WO2011162863A1
公开(公告)日：2011-12-29
申请号：PCT/US2011/032595
申请日：2011-04-15
申请人： WONG, Bing, Lou , KWOK, Sui, Yi
发明人： WONG, Bing, Lou , KWOK, Sui, Yi
IPC分类号： A61K35/14
CPC分类号： A61K38/42
摘要： A highly purified and heat stable cross-linked nonpolymeric tetrameric hemoglobin suitable for use in mammals without causing renal injury and vasoconstriction is provided. A high temperature and short time (HTST) heat processing step is performed to remove undesired dimeric form of hemoglobin, uncross-linked tetrameric hemoglobin, and plasma protein impurities effectively. Addition of N-acetyl cysteine after heat treatment and optionally before heat treatment maintains a low level of met-hemoglobin. The heat stable cross-linked tetrameric hemoglobin can improve and prolong oxygenation in normal and hypoxic tissue. In another aspect, the product is used in the treatment of various types of cancer such as leukemia, colorectal cancer, lung cancer, breast cancer, liver cancer, nasopharyngeal carcinoma and esophageal cancer. The inventive tetrameric hemoglobin can also be used to prevent tumor metastasis and recurrence following surgical tumor excision. Further the inventive tetrameric hemoglobin can be administered to patients prior to chemotherapy and radiation treatment.
摘要翻译：提供了适用于哺乳动物而不引起肾损伤和血管收缩的高度纯化和热稳定的交联的非聚合四聚体血红蛋白。进行高温和短时间（HTST）热处理步骤以有效去除不期望的二聚体形式的血红蛋白，未交联的四聚体血红蛋白和血浆蛋白杂质。热处理后任选地加热N-乙酰半胱氨酸，维持低水平的血红蛋白。热稳定的交联四聚体血红蛋白可以改善并延长正常和缺氧组织的氧合作用。另一方面，该产品用于治疗各种类型的癌症如白血病，结肠直肠癌，肺癌，乳腺癌，肝癌，鼻咽癌和食管癌。本发明的四聚体血红蛋白也可用于预防手术切除肿瘤后的肿瘤转移和复发。此外，本发明的四聚体血红蛋白可以在化疗和放射治疗之前给予患者。

3. 发明申请

WO2011149602A1 HIGH-TEMPERATURE STABLE OXYGEN-CARRIER-CONTAINING PHARMACEUTICAL COMPOSITION 审中-公开
标题翻译：高温稳定的含氧载体的药物组合物
公开(公告)号：WO2011149602A1
公开(公告)日：2011-12-01
申请号：PCT/US2011/032594
申请日：2011-04-15
申请人： WONG, Bing, Lou , KWOK, Sui, Yi
发明人： WONG, Bing, Lou , KWOK, Sui, Yi
IPC分类号： A61K35/14
CPC分类号： C07K14/805
摘要： A high temperature-stable and highly purified cross-linked (optionally > 70% β-β linked) tetrameric hemoglobin with high efficiency of oxygen delivery suitable for use in mammals without causing renal injury and vasoconstriction is provided. The dimeric form of hemoglobin is degenerated and purification processes are performed on red blood cells from whole blood. Controlled hypotonic lysis in an instant cytolysis apparatus prevents lysis of white blood cells. Nucleic acids from white blood cells and phospholipids impurities are not detected. Blocking of reactive sulfhydryl groups by a sulfhydryl reagent is performed in an oxygenated environment. Flowthrough column chromatography removes different plasma protein impurities. N-acetyl cysteine is added to the cross-linked tetrameric hemoglobin to maintain a low level of met-hemoglobin. The stabilized hemoglobin is preserved in an infusion bag with aluminum overwrap to prevent formation of inactive met-hemoglobin from oxygen intrusion. The product finds use in tissue oxygenation and cancer treatment.
摘要翻译：提供了适用于哺乳动物而不引起肾损伤和血管收缩的高温稳定且高度纯化的交联（任选> 70％ß连接的）四聚体血红蛋白，其具有高效率的氧输送。血红蛋白的二聚体形式是退化的，并且从全血对红细胞进行纯化过程。瞬时细胞裂解装置中的受控低渗溶解可防止白细胞裂解。没有检测到白细胞和磷脂杂质的核酸。通过巯基试剂阻断反应性巯基在氧合环境中进行。流式柱色谱法除去不同的血浆蛋白质杂质。将N-乙酰半胱氨酸加入到交联的四聚体血红蛋白中以维持低水平的血红蛋白。将稳定的血红蛋白保存在具有铝外包装的输液袋中，以防止氧气侵入形成无活性的血红蛋白。该产品用于组织氧合和癌症治疗。

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