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1. 发明申请

WO2009058285A2 PORCINE DC-SIGN, ICAM-3 AND LSECtin AND USES THEREOF 审中-公开
标题翻译： PORCINE DC-SIGN，ICAM-3和LSECtin及其用途
公开(公告)号：WO2009058285A2
公开(公告)日：2009-05-07
申请号：PCT/US2008/012251
申请日：2008-10-29
申请人： VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. , HUANG, Yaowei , MENG, Xiang-Jin
发明人： HUANG, Yaowei , MENG, Xiang-Jin
IPC分类号： C07K14/43
CPC分类号： C07K14/70525 , C07K14/7056
摘要： The present invention relates to the cloning, identification and characterization of the unique and entire genomic sequences encoding new porcine DC-SIGN and LSECtin proteins, including the novel nucleotide sequences of the full-length cDNA and genes of both pDC- SIGN gene and pLSECtin. Also provided are the nucleic acid molecules encoding newly discovered porcine ICAM-3 isoforms from porcine monocyte-derived dendritic cells and the use thereof. Specifically, the invention is drawn to an isolated nucleic acid molecule comprising a nucleotide sequence encoding one or more of porcine DC-SIGN, porcine ICAM- 3, porcine LSECtin, a complement of the nucleotide sequence or a functional, defined portion of the nucleotide sequence or a protein fusion product linked to a protein that may be of porcine or human origin. Methods for isolating and cloning the new porcine genes and for using the new nucleotide sequences in improved methods for propagating viruses, particularly enveloped viruses, are additionally described herein. The invention further includes new transfected cells or cell lines that can stably express the porcine proteins, new antibodies and the like.
摘要翻译：本发明涉及克隆，鉴定和表征编码新的猪DC-SIGN和LSECtin蛋白的独特和完整的基因组序列，包括全长cDNA的新型核苷酸序列和 pDC-SIGN基因和pLSECtin。还提供了编码来自猪单核细胞来源的树突细胞的新发现的猪ICAM-3同种型的核酸分子及其用途。具体而言，本发明涉及包含编码猪DC-SIGN，猪ICAM-3，猪LSECtin，核苷酸序列的互补序列或核苷酸序列的功能性限定部分中的一种或多种的核苷酸序列的分离的核酸分子或与可能是猪或人来源的蛋白质连接的蛋白质融合产物。本文额外描述了用于分离和克隆新猪基因的方法以及用于改进繁殖病毒特别是有包膜病毒的方法中的新核苷酸序列的方法。本发明还包括可稳定表达猪蛋白质的新转染细胞或细胞系，新抗体等。

2. 发明申请

WO2011031438A9 PORCINE TORQUE TENO VIRUS VACCINES AND DIAGNOSIS 审中-公开
标题翻译：猪瘟TENO病毒疫苗及其诊断
公开(公告)号：WO2011031438A9
公开(公告)日：2011-03-17
申请号：PCT/US2010/046330
申请日：2010-08-23
申请人： VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. , MENG, Xiang-Jin , HUANG, Yaowei
发明人： MENG, Xiang-Jin , HUANG, Yaowei
IPC分类号： C12N15/34 , C12N15/63 , A61K39/12 , A01K67/027 , C12Q1/68 , G01N33/569
摘要： The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1a-VA, PTTV1b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.
摘要翻译：本发明提供了含有编码猪扭矩型诺沃病毒（PTTV）基因型或亚型PTTV1a-VA，PTTV1b-VA，PTTV2b-VA和PTTV2c-VA的多核酸分子的四种纯化制剂。本发明还提供了感染性DNA克隆，生物功能性质粒或含有其的感染性核酸基因组分子的病毒载体。本发明进一步提供活的，减毒的，载体表达和纯化的重组衣壳亚单位或杀死的病毒疫苗以防止PTTV感染。本发明另外提供了包含PTTV特异性基因产物的亚单位疫苗，特别是用于防止PTTV感染的ORF1衣壳基因产物。此外，本发明提供了使用用于PTTV1，PTTV2和个体PTTV1基因型的特异性引物通过聚合酶链式反应（PCR）诊断PTTV感染的方法。最后，本发明提供了用于通过免疫学方法诊断PTTV感染的方法，例如酶联免疫吸附测定（ELISA）和使用PTTV特异性抗原的Western印迹来检测血清PTTV特异性抗体。

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