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1. 发明申请

WO2009128878A1 CHIMERIC PORCINE CIRCOVIRUS PCV2Gen-1Rep AND USES THEREOF 审中-公开
标题翻译： CHIMERIC PORCINE CIRCOVIRUS PCV2Gen-1Rep及其用途
公开(公告)号：WO2009128878A1
公开(公告)日：2009-10-22
申请号：PCT/US2009/002189
申请日：2009-04-08
申请人： VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. , JUHAN, Nicole, M. , MENG, Xiang-jin
发明人： JUHAN, Nicole, M. , MENG, Xiang-jin
IPC分类号： A61K39/12
CPC分类号： C12N7/00 , A61K2039/525 , C12N2750/10021
摘要： The present invention relates to a novel chimeric nucleic acid molecule of porcine circovirus (PCV2Gen-lRep) that embraces a nucleic acid molecule encoding porcine circovirus type 2 (PCV2) which contains a nucleic acid sequence encoding a Rep protein of porcine circovirus type 1 (PCVl), particularly wherein the nucleic acid sequence encoding the Rep protein of PCVl is an open reading frame (ORF) gene and, more particularly, wherein the ORF Rep gene is ORFl. A highly desirable chimeric nucleic acid molecule is constructed by replacing the ORFl Rep gene of PCV2 by the ORFl Rep gene of PCVl. The invention also encompasses the biologically functional plasmid or viral vector containing the unique chimeric nucleic acid molecules, suitable host cells transfected by the plasmid or vector, infectious chimeric porcine circoviruses that are produced by the suitable host cells, the process for the production of an immunogenic polypeptide product making use of the new chimera, viral vaccines that protect a pig against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2, methods of protecting a pig against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2, methods of preparing the unique chimera of PCV2Gen-lRep and the like. This invention further includes a new method for improving the replication and titer of PCV2 in a cell culture.
摘要翻译：本发明涉及一种包含编码猪圆环病毒2型（PCV2）的核酸分子的猪圆环病毒嵌合核酸分子（PCV2Gen-1Rep），其包含编码猪圆环病毒1型Rep蛋白（PCV1）的核酸序列），特别是其中编码PCV1的Rep蛋白的核酸序列是开放阅读框（ORF）基因，更具体地，其中ORF Rep基因是ORF1。通过用PCV1的ORF1 Rep基因替换PCV2的ORF1 Rep基因来构建非常需要的嵌合核酸分子。本发明还包括含有独特的嵌合核酸分子，由质粒或载体转染的合适的宿主细胞的生物功能质粒或病毒载体，由合适的宿主细胞产生的感染性嵌合猪圆环病毒，产生免疫原性的方法使用新的嵌合体的多肽产物，保护猪免受PCV2引起的病毒感染或断奶后多系统消耗综合征（PMWS）的病毒疫苗，保护猪免受病毒感染或由PCV2引起的断奶后多系统消耗综合征（PMWS）的方法，制备PCV2Gen-lRep等独特嵌合体的方法。本发明还包括用于改善细胞培养物中PCV2的复制和滴度的新方法。

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