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1. 发明申请

WO2010090857A2 METHODS FOR AMPLIFYING HEPATITIS C VIRUS NUCLEIC ACIDS 审中-公开
标题翻译：扩增丙型肝炎病毒核酸的方法
公开(公告)号：WO2010090857A2
公开(公告)日：2010-08-12
申请号：PCT/US2010/021589
申请日：2010-01-21
申请人： VERTEX PHARMACEUTICALS INCORPORATED , KWONG, Ann, D. , FRANTZ, James, Daniel , BARTELS, Douglas, J. , LIN, Chao , SHAMES, Benjamin , SEEPERSAUD, Sheila , LIPPKE, Judith, A. , KIEFFER, Tara, L. , ZHOU, Yi , ZHANG, Eileen, Z. , SULLIVAN, James, C.
发明人： KWONG, Ann, D. , FRANTZ, James, Daniel , BARTELS, Douglas, J. , LIN, Chao , SHAMES, Benjamin , SEEPERSAUD, Sheila , LIPPKE, Judith, A. , KIEFFER, Tara, L. , ZHOU, Yi , ZHANG, Eileen, Z. , SULLIVAN, James, C.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/707 , C12Q1/6883
摘要： A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO: 12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.
摘要翻译：扩增HCV感染样品中的HCV核酸的方法包括通过两阶段PCR扩增与来自样品的HCV RNA基因组互补的DNA模板片段，其中a 第一阶段PCR使用第一外部引物和第二外部引物，并且第二阶段PCR使用第一内部引物和第二内部引物。第一外部引物的核苷酸序列包含如SEQ ID NO：2所示的核苷酸序列; 或SEQ ID NO：9的核苷酸序列，其中任选地1,2或3个核苷酸是除SEQ ID NO：9的核苷酸以外的核苷酸。第二外部引物的核苷酸序列包含SEQ ID NO：3或4所示的核苷酸序列; 或如SEQ ID NO：10或11所示的核苷酸序列，其中任选地1,2或3个核苷酸是与SEQ ID NO：10和11的那些核苷酸不同的核苷酸。第一内部引物的核苷酸序列包含核苷酸序列如SEQ ID NO：5所示; 或SEQ ID NO：12，其中任选地1,2或3个核苷酸是不同于SEQ ID NO：12的核苷酸。第二内部引物的核苷酸序列包含如SEQ ID NO：6或7所示的核苷酸序列 ; 或如SEQ ID NO：13或14所示的核苷酸序列，其中任选地1,2或3个核苷酸是不同于SEQ ID NO：13和14的那些核苷酸。

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