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1. 发明申请

WO2015083002A3 MULTIPLEX DETECTION OF NUCLEIC ACIDS 审中-公开
标题翻译：核酸的多重检测
公开(公告)号：WO2015083002A3
公开(公告)日：2015-11-26
申请号：PCT/IB2014003062
申请日：2014-11-26
申请人： VANADIS DIAGNOSTICS
发明人： DAHL CARL OSCAR FREDRIK , ERICSSON JOHN OLOF
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6816 , C12Q2521/501 , C12Q2525/161 , C12Q2525/307 , C12Q2531/125 , C12Q2533/107 , C12Q2537/143
摘要： Described herein is a new approach in which a nucleic acid species of interest (e.g. a chromosome) containing multiple unique target sequences is detected using multiple specific probes that are amplified by rolling circle amplification and detected. Multiple probes are used to provide a detectable signal, where the magnitude of the signal is proportional to the number of probes recognising their target sequences. Individual signals from the plurality of probes are converted into a single cumulative detectable signal, amplifying the individual signals through the multiplex probing. Ten or more probes produce a signal amplification of ten-fold or more. The generated signals depend on correctly reacted probes upon target recognition, using sequence specific hybridisation and enzymatic catalysis to generate specific products from which the signal is obtained.
摘要翻译：本文描述了一种新方法，其中使用通过滚环扩增和检测扩增的多个特异性探针检测含有多个独特靶序列的目标核酸种类（例如染色体）。使用多个探针来提供可检测信号，其中信号的大小与识别其靶序列的探针的数量成比例。来自多个探测器的各个信号被转换成单个累积可检测信号，通过多路探测放大各个信号。十个或更多个探针产生十倍或更多倍的信号放大。产生的信号取决于靶标识别时正确反应的探针，使用序列特异性杂交和酶促催化以生成从中获得信号的特定产物。

2. 发明申请

WO2015083001A3 NUCLEIC ACID PROBE AND METHOD OF DETECTING GENOMIC FRAGMENTS 审中-公开
标题翻译：核酸探针和检测基因组片段的方法
公开(公告)号：WO2015083001A3
公开(公告)日：2015-11-26
申请号：PCT/IB2014003061
申请日：2014-11-26
申请人： VANADIS DIAGNOSTICS
发明人： DAHL CARL OSCAR FREDRIK , ERICSSON JOHN OLOF
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q2521/501 , C12Q2525/161 , C12Q2525/307 , C12Q2531/125
摘要： Provided herein, among other things, is a method of processing a nucleic acid sample. In some embodiments, the method comprises a) hybridizing a sample comprising a target fragment to a nucleic acid probe comprising: i. a head sequence and a tail sequence, wherein the head and tail sequences are at the ends of a first oligonucleotide molecule; and ii. a splint sequence comprising, in order: an upstream flanking sequence that is complementary to the head sequence; a target complementary sequence that is complementary to the target fragment; and a downstream flanking sequence that is complementary to the tail sequence; thereby producing a hybridization product in which the ends of the target fragment are ligatably adjacent to the ends of the head and tail sequences in the first oligonucleotide molecule; and b) ligating the ends of the target fragment to the ends of the head and tail sequences of the first oligonucleotide molecule, thereby producing a cyclic product that comprises the target fragment and the head and tail sequences. Probes and kits for performing the method are also provided.
摘要翻译：本文提供的是一种处理核酸样品的方法。在一些实施方案中，所述方法包括：a）将包含靶片段的样品与核酸探针杂交，所述核酸探针包含：头序列和尾序列，其中头序列和尾序列位于第一寡核苷酸分子的末端; 和ii。夹板序列，其顺序包括：与头序列互补的上游侧翼序列; 与靶片段互补的靶互补序列; 和与尾序列互补的下游侧翼序列; 从而产生杂交产物，其中靶片段的末端可与第一寡核苷酸分子中的头尾序列的末端相连; 和b）将靶片段的末端连接到第一寡核苷酸分子的头尾序列的末端，从而产生包含靶片段和头尾序列的环状产物。还提供了用于执行该方法的探针和试剂盒。

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