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1. 发明申请

WO2010058418A3 NOVEL SYNTHETIC EXPRESSION VEHICLE 审中-公开
标题翻译：新型合成表情车
公开(公告)号：WO2010058418A3
公开(公告)日：2010-09-30
申请号：PCT/IN2009000610
申请日：2009-10-28
申请人： USV LTD , RAO LAXMI SRINIVAS , MISHRA SHRIKANT , NIPHADKAR MILIND , THAKUR PRITI , NAZARETH GENEVIEVE
发明人： RAO LAXMI SRINIVAS , MISHRA SHRIKANT , NIPHADKAR MILIND , THAKUR PRITI , NAZARETH GENEVIEVE
IPC分类号： C12N15/70 , C12R1/19
CPC分类号： C12N15/70
摘要： An expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 comprising of a synthetic hybrid promoter wherein the hybrid promoter comprises of an inducible arabinose promoter derived from E.coli and a synthetic stretch of 8-35 nucleotides from pHO regulon introduced in the region between -55 to -10 and ribosome binding site from the transcription initiation site.
摘要翻译：包含如Seq ID No.1所示的分离的核酸的表达载体，其包含合成的杂合启动子，其中所述杂合启动子包含来源于大肠杆菌的诱导型阿拉伯糖启动子和来自pHO调节子的8-35个核苷酸的合成片段在-55至-10之间的区域和与转录起始位点的核糖体结合位点。

2. 发明申请

WO2009005973A3 SYNTHETIC GENE FOR ENHANCED EXPRESSION IN E.COLI 审中-公开
标题翻译：在E.COLI中增强表达的合成基因
公开(公告)号：WO2009005973A3
公开(公告)日：2009-12-30
申请号：PCT/US2008066496
申请日：2008-06-11
申请人： PHARMACEUTICAL PATENT ATTORNEY , RAO LAXMI SRINIVAS , AHMED MONSUR BORBHUIYA , NIPHADKAR MILIND , PALIWAL DINESH , SHEKHAWAT RAKESH , KHARE ARUNA , SAMANT RADHIKA , THAKUR PRITI , CHUTKE ANJALI , SURLIKAR NILESH , ZAWAR SAGAR , MISHRA SHRIKANT , USV LTD
发明人： RAO LAXMI SRINIVAS , AHMED MONSUR BORBHUIYA , NIPHADKAR MILIND , PALIWAL DINESH , SHEKHAWAT RAKESH , KHARE ARUNA , SAMANT RADHIKA , THAKUR PRITI , CHUTKE ANJALI , SURLIKAR NILESH , ZAWAR SAGAR , MISHRA SHRIKANT
IPC分类号： C12P21/04
CPC分类号： C12P21/02 , C07K14/58
摘要： A novel nesiritide synthetic cDNA chimera encoding human b-type natriuretic peptide (hBNP) or nesiritide and a process for the preparation of the said novel chimera. Further, the inventors disclose the use of nesiritide synthetic cDNA chimera to obtain an expressible construct to produce mature nesiritide. Particularly, the inventors disclose an application of recombinant cloning method to prepare an ORF of a nesiritide chimeric construct, which is simultaneously codon optimized for E.coli and has optimal RNA stability. The inventors also provide a process for large scale purification of nesiritide by pH precipitation and chromatography.
摘要翻译：编码人b型利尿钠肽（hBNP）或奈西立肽的新型奈西立肽合成cDNA嵌合体以及制备所述新嵌合体的方法。此外，本发明人公开了使用奈西立肽合成cDNA嵌合体获得可表达的构建体以产生成熟的奈西立肽。具体而言，本发明人公开了应用重组克隆方法来制备奈西立肽嵌合构建体的ORF，其对大肠杆菌同时密码子优化并且具有最佳的RNA稳定性。本发明人还提供了通过pH沉淀和色谱法大规模纯化奈西立肽的方法。

3. 发明申请

WO2007096899A3 AFFINITY POLYPEPTIDE FOR PURIFICATION OF RECOMBINANT PROTEINS 审中-公开
标题翻译：用于纯化重组蛋白的亲和聚合物（AFFINITY POLYPEPTIDE FOR PURIFICATION OF RECOMBINANT PROTEINS）
公开(公告)号：WO2007096899A3
公开(公告)日：2008-03-13
申请号：PCT/IN2007000051
申请日：2007-02-07
申请人： USV LTD , RAO LAXMI S , MISHRA SHRIKANT , BORBHUIYA MONSUR AHMED , MHATRE DEEPA , THAKUR PRITI
发明人： RAO LAXMI S , MISHRA SHRIKANT , BORBHUIYA MONSUR AHMED , MHATRE DEEPA , THAKUR PRITI
IPC分类号： C12N15/62
CPC分类号： C12N15/62
摘要： The present disclosure provides an affinity polypeptide for the purification of a recombinant biologically active protein or polypeptide. Further, the present disclosure provides a fusion recombinant protein or polypeptide wherein the fusion recombinant protein comprises of at least two components, a biologically active polypeptide or protein or protein of interest and the affinity polypeptide. The biologically active polypeptide may be linked directly or indirectly to the affinity polypeptide by covalent binding. The present disclosure provides a recombinant expression vector for the producing said fusion recombinant protein in host cells. Further, the present disclosure provides an improved method of purification of recombinant protein from the host cells. Further, the disclosure provides a method of purification of the recombinant biologically active polypeptide or protein by immobilized metal ion chelating chromatography.
摘要翻译：本公开提供了用于纯化重组生物活性蛋白或多肽的亲和多肽。此外，本公开提供了融合重组蛋白或多肽，其中融合重组蛋白包含至少两种组分，生物活性多肽或感兴趣的蛋白质或亲和性多肽。生物活性多肽可以通过共价结合直接或间接地连接到亲和多肽上。本公开内容提供了用于在宿主细胞中产生所述融合重组蛋白的重组表达载体。此外，本公开提供了从宿主细胞纯化重组蛋白质的改进方法。此外，本公开提供了通过固定金属离子螯合色谱法纯化重组生物活性多肽或蛋白质的方法。

4. 发明申请

WO2005121164A9 PROCESS FOR THE PREPARATION OF PEPTIDES 审中-公开
标题翻译：制备肽的方法
公开(公告)号：WO2005121164A9
公开(公告)日：2006-03-02
申请号：PCT/IN2004000315
申请日：2004-10-10
申请人： USV LTD , SAKSENA DIVYA LAL , MISHRA SHRIKANT , MURALIDHARAN CHANDRAKESAN , THAKARE MILIND , PATIL NILESH , KHARE ARUNA
发明人： SAKSENA DIVYA LAL , MISHRA SHRIKANT , MURALIDHARAN CHANDRAKESAN , THAKARE MILIND , PATIL NILESH , KHARE ARUNA
IPC分类号： C07K1/00 , C07K1/06 , C07K7/06
CPC分类号： C07K7/06 , Y02P20/55
摘要： The present invention relates to an improved process for the preparation of N6-(aminoiminomethyl)-N2-(3-mercapto-1-oxopropyl-L-lysylglycyl-L- -aspartyl-L-tryptophyl-L-prolyl-L-cysteinamide, cyclic(1 6)-disulfide of formula (1), which involves assembling amino acid residues and a thioalkyl carboxylic acid with appropriate protecting groups on a solid phase resin, cleaving the peptide thus obtained from the resin with concomitant removal of side chain protecting groups except Acm protecting group of thiol moiety to obtain peptide amide of formula(3), converting lysine residue of peptide amide of formula(3) having protected thiol group to homoarginine residue by guanylation to obtain peptide amide of formula(4), preparation of silver peptide of formula(5), followed by simultaneous deprotection and oxidation of the said silver peptide to obtain crude peptide amide comprising compound of formula(1) and finally subjecting to chromatographic purification. The described process is simple, easy, environment friendly and cost effective.
摘要翻译：本发明涉及一种制备N6-（氨基亚氨基甲基）-N2-（3-巯基-1-氧代丙基-L-赖氨酰甘氨酰基-L-天冬酰基-L-色氨酰-L-脯氨酰基-L-半胱氨酰胺，式（1）的环状（16）二硫化物，其包括将固定相树脂上的氨基酸残基和硫代烷基羧酸与合适的保护基团组装，从树脂上切下由此获得的肽，同时除去侧链保护基除了巯基部分的Acm保护基以获得式（3）的肽酰胺，通过胍基将具有保护的硫醇基的式（3）的肽酰胺的赖氨酸残基转化成高精氨酸残基，得到式（4）的肽酰胺，制备银然后将所述银肽同时去保护和氧化，得到包含式（1）化合物的粗肽酰胺，最后进行色谱纯化，所述方法简单，容易环保，性价比高。

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