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1. 发明申请

WO2007096899A2 AFFINITY POLYPEPTIDE FOR PURIFICATION OF RECOMBINANT PROTEINS 审中-公开
标题翻译：用于纯化重组蛋白的亲和聚合物（AFFINITY POLYPEPTIDE FOR PURIFICATION OF RECOMBINANT PROTEINS）
公开(公告)号：WO2007096899A2
公开(公告)日：2007-08-30
申请号：PCT/IN2007/000051
申请日：2007-02-07
申请人： USV LIMITED , RAO, Laxmi, S. , MISHRA, Shrikant , BORBHUIYA, Monsur, Ahmed , MHATRE, Deepa , THAKUR, Priti
发明人： RAO, Laxmi, S. , MISHRA, Shrikant , BORBHUIYA, Monsur, Ahmed , MHATRE, Deepa , THAKUR, Priti
IPC分类号： C07K14/43
CPC分类号： C12N15/62
摘要： The present disclosure provides an affinity polypeptide for the purification of a recombinant biologically active protein or polypeptide. Further, the present disclosure provides a fusion recombinant protein or polypeptide wherein the fusion recombinant protein comprises of at least two components, a biologically active polypeptide or protein or protein of interest and the affinity polypeptide. The biologically active polypeptide may be linked directly or indirectly to the affinity polypeptide by covalent binding. The present disclosure provides a recombinant expression vector for the producing said fusion recombinant protein in host cells. Further, the present disclosure provides an improved method of purification of recombinant protein from the host cells. Further, the disclosure provides a method of purification of the recombinant biologically active polypeptide or protein by immobilized metal ion chelating chromatography.
摘要翻译：本公开提供了用于纯化重组生物活性蛋白或多肽的亲和多肽。此外，本公开提供了融合重组蛋白或多肽，其中融合重组蛋白包含至少两种组分，生物活性多肽或感兴趣的蛋白质或亲和性多肽。生物活性多肽可以通过共价结合直接或间接地连接到亲和多肽上。本公开内容提供了用于在宿主细胞中产生所述融合重组蛋白的重组表达载体。此外，本公开提供了从宿主细胞纯化重组蛋白质的改进方法。此外，本公开提供了通过固定金属离子螯合色谱法纯化重组生物活性多肽或蛋白质的方法。

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